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Protein

Glutamine synthetase

Gene

glnA

Organism
Microchaete diplosiphon (Fremyella diplosiphon)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Magnesium 1By similarity1
Metal bindingi133Magnesium 2By similarity1
Binding sitei208ATPBy similarity1
Metal bindingi213Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Binding sitei266L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi270Magnesium 1; via pros nitrogenBy similarity1
Binding sitei274ATPBy similarity1
Binding sitei322L-glutamateBy similarity1
Binding sitei328L-glutamateBy similarity1
Binding sitei340ATPBy similarity1
Binding sitei340L-glutamateBy similarity1
Binding sitei345ATPBy similarity1
Binding sitei354ATPBy similarity1
Metal bindingi359Magnesium 1By similarity1
Binding sitei361L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 274ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiMicrochaete diplosiphon (Fremyella diplosiphon)
Taxonomic identifieri1197 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesRivulariaceaeMicrochaete

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532162 – 471Glutamine synthetaseAdd BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei399O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Structurei

3D structure databases

ProteinModelPortaliP33035.
SMRiP33035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 266L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P33035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQEVLKL IQDQKIQMID LKFIDTPGTW QHLTVYYNQI DESSFTDGVP
60 70 80 90 100
FDGSSIRGWK GIEESDMTMV LDPNTAWIDP FMKEPTLSII CSIKEPRTGE
110 120 130 140 150
WYNRCPRVIA QKAIDYLVST GLGDTAFFGP EAEFFIFDDA RFDQTANSGY
160 170 180 190 200
YYVDSVEGRW NSGKDEGPNL AYKPRFKEGY FPVAPTDTFQ DMRTEMLLTM
210 220 230 240 250
AACGVPIEKQ HHEVATGGQC ELGFRFGKLI EAADWLMTYK YVIKNVAKKY
260 270 280 290 300
GRTVTFMPKP IFGDNGSGMH CHQSIWKDGK PLFGGDKYAG LSDMALYYIG
310 320 330 340 350
GILKHAPALL GITNPTTNSY KRLVPGYEAP VNLAYSQGNR SASVRIPLSG
360 370 380 390 400
TNPKAKRLEF RCPDATSNPY LAFAAMLCAG IDGIKNKIHP GEPLDRNIYE
410 420 430 440 450
LSPEELAKVP STPGSLELAL EALENDHAFL TESGVFTEDF IQNWIEYKLV
460 470
NEVKQLQLRP HPYEFYLYYD C
Length:471
Mass (Da):53,050
Last modified:January 23, 2007 - v2
Checksum:i4216D115B6EC7310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05609 Genomic DNA. Translation: AAA23288.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1B_MICDP
AccessioniPrimary (citable) accession number: P33035
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: April 12, 2017
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families