P33034 (TOG3A_AGEAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Omega-agatoxin-Aa3a Short name=Omega-AGTX-Aa3a Alternative name(s): Omega-agatoxin IIIA Short name=Omega-Aga-IIIA Omega-agatoxin-3A
Organism	Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifier	6908 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › RTA clade › Agelenidae › Agelenopsis

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Omega-agatoxin are antagonist of voltage-gated calcium channels. They block insect neuromuscular transmission presynaptically. Potent blocker of N- (Cav2.2/CACNA1B) and L-type (Cav1/CACNA1) calcium channels.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin Probable.
Sequence similarities	Belongs to the omega-agatoxin superfamily. Type II/III omega-agatoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Calcium channel inhibitor Ion channel impairing toxin Neurotoxin Presynaptic neurotoxin Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	other organism presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	Omega-agatoxin-Aa3a		PRO_0000087606
Amino acid modifications
	Disulfide bond	2 ↔ 19		Probable
	Disulfide bond	9 ↔ 25		Probable
	Disulfide bond	16 ↔ 52		Probable
	Disulfide bond	18 ↔ 40		Probable
	Disulfide bond	27 ↔ 38		Probable
	Disulfide bond	59 ↔ 67		Probable
Natural variations
	Natural variant	58	1	Q → T.

Sequences

	Sequence		Length	Mass (Da)
	P33034 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 6A715CAC9591888B	FASTA	76	8,518
	10 20 30 40 50 60 SCIDIGGDCD GEKDDCQCCR RNGYCSCYSL FGYLKSGCKC VVGTSAEFQG ICRRKARQCY 70 NSDPDKCESH NKPKRR

References

[1]	"Antagonism of synaptosomal calcium channels by subtypes of omega-agatoxins." Venema V.J., Swiderek K.M., Lee T.D., Hathaway G.M., Adams M.E. J. Biol. Chem. 267:2610-2615(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	A42335. A54252.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ArachnoServer	AS000178. omega-agatoxin-Aa3a.
Family and domain databases
InterPro	IPR005853. Omega-agatoxin_II/III_CS. IPR013605. Toxin_34. [Graphical view]
Pfam	PF08396. Toxin_34. 1 hit. [Graphical view]
PROSITE	PS60023. OMEGA_AGA_II_III. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOG3A_AGEAP

Accession

Primary (citable) accession number: P33034

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program