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Protein

Zinc-binding GTPase YeiR

Gene

yeiR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in metal homeostasis. Has GTPase activity. Binds several Zn2+ ions in vitro.1 Publication

Enzyme regulationi

GTPase activity is enhanced by Zn2+ binding.1 Publication

Kineticsi

kcat is 0.19 min(-1). kcat is 0.33 min(-1) in the presence of Ni2+. kcat is 0.32 min(-1) in the presence of Zn2+.1 Publication

Manual assertion based on experiment ini

  1. KM=220 µM for GTP (in the absence of metal ions)1 Publication
  2. KM=177 µM for GTP (in the presence of Ni2+)1 Publication
  3. KM=49 µM for GTP (in the presence of Zn2+)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei155GTPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi9 – 17GTPBy similarity9

    GO - Molecular functioni

    • GTPase activity Source: EcoCyc
    • GTP binding Source: UniProtKB-KW
    • zinc ion binding Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12104-MONOMER.
    ECOL316407:JW2161-MONOMER.
    MetaCyc:EG12104-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc-binding GTPase YeiRCurated (EC:3.6.-.-)
    Gene namesi
    Name:yeiR
    Ordered Locus Names:b2173, JW2161
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12104. yeiR.

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of the gene increases the sensitivity of E.coli to the metal chelator EDTA and sensitivity to cadmium.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001691511 – 328Zinc-binding GTPase YeiRAdd BLAST328

    Proteomic databases

    PaxDbiP33030.
    PRIDEiP33030.

    Interactioni

    Subunit structurei

    Oligomerizes in the presence of Zn2+.1 Publication

    Protein-protein interaction databases

    BioGridi4260468. 311 interactors.
    DIPiDIP-11929N.
    IntActiP33030. 14 interactors.
    MINTiMINT-1231253.
    STRINGi511145.b2173.

    Structurei

    3D structure databases

    ProteinModelPortaliP33030.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini241 – 321CobW C-terminalSequence analysisAdd BLAST81

    Sequence similaritiesi

    Contains 1 cobW C-terminal domain.Sequence analysis

    Phylogenomic databases

    eggNOGiENOG4105CKI. Bacteria.
    COG0523. LUCA.
    HOGENOMiHOG000294929.
    InParanoidiP33030.
    OMAiCICCTAN.
    PhylomeDBiP33030.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR011629. Cbl_biosynth_CobW-like_C.
    IPR003495. CobW/HypB/UreG_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF02492. cobW. 1 hit.
    PF07683. CobW_C. 1 hit.
    [Graphical view]
    SMARTiSM00833. CobW_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P33030-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTRTNLITGF LGSGKTTSIL HLLAHKDPNE KWAVLVNEFG EVGIDGALLA
    60 70 80 90 100
    DSGALLKEIP GGCMCCVNGL PMQVGLNTLL RQGKPDRLLI EPTGLGHPKQ
    110 120 130 140 150
    ILDLLTAPVY EPWIDLRATL CILDPRLLLD EKSASNENFR DQLAAADIIV
    160 170 180 190 200
    ANKSDRTTPE SEQALQRWWQ QNGGDRQLIH SEHGKVDGHL LDLPRRNLAE
    210 220 230 240 250
    LPASAAHSHQ HVVKKGLAAL SLPEHQRWRR SLNSGQGYQA CGWIFDADTV
    260 270 280 290 300
    FDTIGILEWA RLAPVERVKG VLRIPEGLVR INRQGDDLHI ETQNVAPPDS
    310 320
    RIELISSSEA DWNALQSALL KLRLATTA
    Length:328
    Mass (Da):36,113
    Last modified:November 1, 1997 - v2
    Checksum:i83C542DE4E0B4DA6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti103 – 105DLL → LSF in AAA60521 (Ref. 4) Curated3
    Sequence conflicti285 – 328GDDLH…LATTA → AMTCTLKRKTLRHRTAVLS (PubMed:9097040).CuratedAdd BLAST44

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75234.1.
    AP009048 Genomic DNA. Translation: BAA15982.2.
    U00007 Genomic DNA. Translation: AAA60521.2.
    PIRiD64986.
    RefSeqiNP_416678.1. NC_000913.3.
    WP_000198828.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75234; AAC75234; b2173.
    BAA15982; BAA15982; BAA15982.
    GeneIDi946701.
    KEGGiecj:JW2161.
    eco:b2173.
    PATRICi32119701. VBIEscCol129921_2261.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75234.1.
    AP009048 Genomic DNA. Translation: BAA15982.2.
    U00007 Genomic DNA. Translation: AAA60521.2.
    PIRiD64986.
    RefSeqiNP_416678.1. NC_000913.3.
    WP_000198828.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP33030.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260468. 311 interactors.
    DIPiDIP-11929N.
    IntActiP33030. 14 interactors.
    MINTiMINT-1231253.
    STRINGi511145.b2173.

    Proteomic databases

    PaxDbiP33030.
    PRIDEiP33030.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75234; AAC75234; b2173.
    BAA15982; BAA15982; BAA15982.
    GeneIDi946701.
    KEGGiecj:JW2161.
    eco:b2173.
    PATRICi32119701. VBIEscCol129921_2261.

    Organism-specific databases

    EchoBASEiEB2028.
    EcoGeneiEG12104. yeiR.

    Phylogenomic databases

    eggNOGiENOG4105CKI. Bacteria.
    COG0523. LUCA.
    HOGENOMiHOG000294929.
    InParanoidiP33030.
    OMAiCICCTAN.
    PhylomeDBiP33030.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12104-MONOMER.
    ECOL316407:JW2161-MONOMER.
    MetaCyc:EG12104-MONOMER.

    Miscellaneous databases

    PROiP33030.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR011629. Cbl_biosynth_CobW-like_C.
    IPR003495. CobW/HypB/UreG_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF02492. cobW. 1 hit.
    PF07683. CobW_C. 1 hit.
    [Graphical view]
    SMARTiSM00833. CobW_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYEIR_ECOLI
    AccessioniPrimary (citable) accession number: P33030
    Secondary accession number(s): P76444, P94761
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: November 1, 1997
    Last modified: November 2, 2016
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.