Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA gyrase inhibitor

Gene

sbmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the supercoiling activity of DNA gyrase. Acts by inhibiting DNA gyrase at an early step, prior to (or at the step of) binding of DNA by the gyrase. It protects cells against toxins that target DNA gyrase, by inhibiting activity of these toxins and reducing the formation of lethal double-strand breaks in the cell. Protects cells against the natural plasmid-encoded toxins microcin B17 (MccB17) and CcdB, and synthetic quinolones. Can also protect cells against alkylating agents that act independently of DNA gyrase, suggesting a more general role in protecting cells against DNA damage.4 Publications

GO - Molecular functioni

  • DNA topoisomerase (ATP-hydrolyzing) inhibitor activity Source: CACAO
  • enzyme binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • negative regulation of DNA-dependent DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG11892-MONOMER.
ECOL316407:JW1991-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase inhibitor
Gene namesi
Name:sbmC
Synonyms:gyrI, yeeB
Ordered Locus Names:b2009, JW1991
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11892. sbmC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157DNA gyrase inhibitorPRO_0000083882Add
BLAST

Proteomic databases

EPDiP33012.
PaxDbiP33012.
PRIDEiP33012.

Expressioni

Inductioni

By DNA-damaging agents and by the entry of cells into the stationary growth phase.1 Publication

Interactioni

Subunit structurei

Interacts with DNA gyrase. Interacts preferentially with the holoenzyme composed of GyrA and GyrB, but can also weakly interact with the individual GyrA and GyrB subunits.1 Publication

GO - Molecular functioni

  • enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260412. 37 interactions.
DIPiDIP-9862N.
IntActiP33012. 1 interaction.
MINTiMINT-226165.
STRINGi511145.b2009.

Structurei

Secondary structure

1
157
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Beta strandi11 – 2010Combined sources
Helixi22 – 3918Combined sources
Beta strandi45 – 517Combined sources
Turni55 – 573Combined sources
Helixi60 – 623Combined sources
Beta strandi64 – 718Combined sources
Beta strandi85 – 895Combined sources
Beta strandi92 – 10110Combined sources
Helixi107 – 11711Combined sources
Beta strandi121 – 1255Combined sources
Beta strandi130 – 1345Combined sources
Helixi138 – 1414Combined sources
Beta strandi142 – 15413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JYHX-ray1.80A1-157[»]
ProteinModelPortaliP33012.
SMRiP33012. Positions 1-155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33012.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 968Required for interaction with DNA gyrase and inhibition of its activity

Sequence similaritiesi

Belongs to the DNA gyrase inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG4108S5C. Bacteria.
COG3449. LUCA.
HOGENOMiHOG000121896.
InParanoidiP33012.
KOiK07470.
OMAiTPWYQFF.
OrthoDBiEOG6Z3KJW.

Family and domain databases

Gene3Di3.20.80.10. 1 hit.
HAMAPiMF_01896. DNA_gyrase_inhibitor.
InterProiIPR010499. AraC_E-bd.
IPR024911. DNA_gyrase_inhibitor_GyrI.
IPR029442. GyrI-like.
IPR011256. Reg_factor_effector_dom.
[Graphical view]
PfamiPF06445. GyrI-like. 1 hit.
[Graphical view]
SMARTiSM00871. AraC_E_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55136. SSF55136. 1 hit.

Sequencei

Sequence statusi: Complete.

P33012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYEIKQEEK RTVAGFHLVG PWEQTVKKGF EQLMMWVDSK NIVPKEWVAV
60 70 80 90 100
YYDNPDETPA EKLRCDTVVT VPGYFTLPEN SEGVILTEIT GGQYAVAVAR
110 120 130 140 150
VVGDDFAKPW YQFFNSLLQD SAYEMLPKPC FEVYLNNGAE DGYWDIEMYV

AVQPKHH
Length:157
Mass (Da):18,081
Last modified:February 1, 1994 - v1
Checksum:iC85BCD23F28C274E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84885 Genomic DNA. Translation: CAA59312.1.
U00009 Genomic DNA. Translation: AAA16415.1.
U00096 Genomic DNA. Translation: AAC75070.1.
AP009048 Genomic DNA. Translation: BAA15830.1.
PIRiH64965.
RefSeqiNP_416513.1. NC_000913.3.
WP_001105415.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75070; AAC75070; b2009.
BAA15830; BAA15830; BAA15830.
GeneIDi946546.
KEGGiecj:JW1991.
eco:b2009.
PATRICi32119353. VBIEscCol129921_2086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84885 Genomic DNA. Translation: CAA59312.1.
U00009 Genomic DNA. Translation: AAA16415.1.
U00096 Genomic DNA. Translation: AAC75070.1.
AP009048 Genomic DNA. Translation: BAA15830.1.
PIRiH64965.
RefSeqiNP_416513.1. NC_000913.3.
WP_001105415.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JYHX-ray1.80A1-157[»]
ProteinModelPortaliP33012.
SMRiP33012. Positions 1-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260412. 37 interactions.
DIPiDIP-9862N.
IntActiP33012. 1 interaction.
MINTiMINT-226165.
STRINGi511145.b2009.

Proteomic databases

EPDiP33012.
PaxDbiP33012.
PRIDEiP33012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75070; AAC75070; b2009.
BAA15830; BAA15830; BAA15830.
GeneIDi946546.
KEGGiecj:JW1991.
eco:b2009.
PATRICi32119353. VBIEscCol129921_2086.

Organism-specific databases

EchoBASEiEB1838.
EcoGeneiEG11892. sbmC.

Phylogenomic databases

eggNOGiENOG4108S5C. Bacteria.
COG3449. LUCA.
HOGENOMiHOG000121896.
InParanoidiP33012.
KOiK07470.
OMAiTPWYQFF.
OrthoDBiEOG6Z3KJW.

Enzyme and pathway databases

BioCyciEcoCyc:EG11892-MONOMER.
ECOL316407:JW1991-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP33012.
PROiP33012.

Family and domain databases

Gene3Di3.20.80.10. 1 hit.
HAMAPiMF_01896. DNA_gyrase_inhibitor.
InterProiIPR010499. AraC_E-bd.
IPR024911. DNA_gyrase_inhibitor_GyrI.
IPR029442. GyrI-like.
IPR011256. Reg_factor_effector_dom.
[Graphical view]
PfamiPF06445. GyrI-like. 1 hit.
[Graphical view]
SMARTiSM00871. AraC_E_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55136. SSF55136. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "sbmC, a stationary-phase induced SOS Escherichia coli gene, whose product protects cells from the DNA replication inhibitor microcin B17."
    Baquero M.-R., Bouzon M., Varea J., Moreno F.
    Mol. Microbiol. 18:301-311(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
    Strain: RYC1000.
  2. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Identification of DNA gyrase inhibitor (GyrI) in Escherichia coli."
    Nakanishi A., Oshida T., Matsushita T., Imajoh-Ohmi S., Ohnuki T.
    J. Biol. Chem. 273:1933-1938(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-16, FUNCTION, CHARACTERIZATION.
  7. "GyrI: a counter-defensive strategy against proteinaceous inhibitors of DNA gyrase."
    Chatterji M., Nagaraja V.
    EMBO Rep. 3:261-267(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Characterization of the interaction between DNA gyrase inhibitor and DNA gyrase of Escherichia coli."
    Nakanishi A., Imajoh-Ohmi S., Hanaoka F.
    J. Biol. Chem. 277:8949-8954(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DNA GYRASE.
  9. "Chromosomally encoded gyrase inhibitor GyrI protects Escherichia coli against DNA-damaging agents."
    Chatterji M., Sengupta S., Nagaraja V.
    Arch. Microbiol. 180:339-346(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Crystal structure of the Escherichia coli SbmC protein that protects cells from the DNA replication inhibitor microcin B17."
    Romanowski M.J., Gibney S.A., Burley S.K.
    Proteins 47:403-407(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiSBMC_ECOLI
AccessioniPrimary (citable) accession number: P33012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.