Reviewed, UniProtKB/Swiss-Prot P32989 (TOP1_VARV)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:	TOP1
ORF Names:	H6R, I6R

Organism

Variola virus

Taxonomic identifier

10255 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Developmental stage	Late protein
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 314

314

DNA topoisomerase 1

PRO_0000145217

Sites

Active site

274

1

O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Secondary structure

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314

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Beta strand

10 – 14

5

Helix

27 – 33

7

Beta strand

41 – 48

8

Helix

50 – 53

4

Beta strand

56 – 62

7

Beta strand

68 – 72

5

Helix

74 – 105

32

Helix

113 – 127

15

Helix

134 – 140

7

Helix

150 – 152

3

Beta strand

153 – 156

4

Beta strand

159 – 165

7

Helix

167 – 169

3

Beta strand

171 – 177

7

Helix

183 – 189

7

Beta strand

197 – 200

4

Helix

205 – 212

8

Helix

213 – 215

3

Helix

219 – 241

23

Helix

248 – 263

16

Turn

267 – 269

3

Helix

270 – 273

4

Helix

276 – 282

7

Helix

288 – 293

6

Helix

297 – 311

15

Sequences

	Sequence		Length	Mass (Da)
	P32989-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: AE9C7DC5FA363BEE	FASTA	314	36,592
	10 20 30 40 50 60 MRALFYKDGK LFTDNNFLNP VSDNNPAYEV LQHVKIPTHL TDVVVYGQTW EEALTRLIFV 70 80 90 100 110 120 GSDSKGRRQY FYGKMHVQNR NAKRDRIFVR VYNVMKRINC FINKNIKKSS TDSNYQLAVF 130 140 150 160 170 180 MLMETMFFIR FGKMKYLKEN ETVGLLTLKN KHIEISPDKI VIKFVGKDKV SHEFVVHKSN 190 200 210 220 230 240 RLYKPLLKLT DDSSPEEFLF NKLSERKVYE CIKQFGIRIK DLRTYGVNYT FLYNFWTNVK 250 260 270 280 290 300 SISPLPSPKK LIALTIKQTA EVVGHTPSIS KRAYMATTIL EMVKDKNFLD VVSKTTFDEF 310 LSIVVDHVKS STDG

References

[1]	"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments." Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S. Virus Res. 27:25-35(1993) [PubMed: 8383392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed: 8384129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X67119 Genomic DNA. Translation: CAA47588.1.
S55844 Genomic DNA. Translation: AAB24685.1.
X69198 Genomic DNA. Translation: CAA49030.1.

PIR

E36846.

RefSeq

NP_042133.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H7F	X-ray	2.70	X	1-314	[»]
2H7G	X-ray	1.90	X	1-314	[»]

ModBase

Search...

Genome annotation databases

GeneID

1486444.

Enzyme and pathway databases

BRENDA

5.99.1.2. 288297.

Family and domain databases

InterPro

IPR018521. TopoI_AS.
IPR001631. TopoI_C.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]

Gene3D

G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit.

Pfam

PF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]

PRINTS

PR00416. EUTPISMRASEI.

PROSITE

PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TOP1_VARV

Accession

Primary (citable) accession number: P32989

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)