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P32984 (RIR1_VAR67) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase large subunit

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene names
ORF Names:I4L, K4L, ORF3L
OrganismVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]
Taxonomic identifier587200 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length771 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulation

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Induction

Expressed early in the viral replicative cycle.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Contains 1 ATP-cone domain.

Ontologies

Keywords
   Biological processDNA replication
   Developmental stageEarly protein
   LigandATP-binding
Nucleotide-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentribonucleoside-diphosphate reductase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 771771Ribonucleoside-diphosphate reductase large subunit
PRO_0000187233

Regions

Domain1 – 9292ATP-cone
Region217 – 2182Substrate binding By similarity
Region427 – 4315Substrate binding By similarity
Region602 – 6065Substrate binding By similarity

Sites

Active site4271Proton acceptor By similarity
Active site4291Cysteine radical intermediate By similarity
Active site4311Proton acceptor By similarity
Binding site2021Substrate By similarity
Binding site2471Substrate; via amide nitrogen By similarity
Site2181Important for hydrogen atom transfer By similarity
Site2261Allosteric effector binding By similarity
Site2561Allosteric effector binding By similarity
Site4441Important for hydrogen atom transfer By similarity
Site7351Important for electron transfer By similarity
Site7361Important for electron transfer By similarity
Site7661Interacts with thioredoxin/glutaredoxin By similarity
Site7691Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond218 ↔ 444Redox-active By similarity

Natural variations

Natural variant5091D → Y in strain: Garcia-1966.
Natural variant7641E → K in strain: Garcia-1966.

Sequences

Sequence LengthMass (Da)Tools
P32984 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E628AAA9AD95A8CD

FASTA77187,761
        10         20         30         40         50         60 
MFVIKRNGYK ENVMFDKITS RIRKLCYGLN TDHIDPIKIA MKVIQGIYNG VTTVELDTLT 

        70         80         90        100        110        120 
AEIAATCTTQ HPDYAILAAR IAVSNLHKET KKLFSEVMKD LFNYVNPKNG KHSPIISSIT 

       130        140        150        160        170        180 
MDVVNKYKDK LNSVIIYERD FSYNYFGFKT LEKSYLLKIN NKIVERPQHM LMRVAVGIHQ 

       190        200        210        220        230        240 
WDIDSAIETY NLLSEKWFTH ASPTLFNAGT SRHQMSSCFL LNMMDDSIEG IYDTLKRCAL 

       250        260        270        280        290        300 
ISKMAGGIGL SISNIRASGS YISGTNGASN GIIPMLRVYN NTARYIDQGG NKRPGVMTIY 

       310        320        330        340        350        360 
LEPWHSDIMA FLDLKKNTGN EEHRTRDLFI ALWIPDLFMK RVKDDGEWSL MCPDECPGLD 

       370        380        390        400        410        420 
NVWGDEFERL YTLYEREKRY KSIIKARVVW KAIIESQIET GTPFILYKDA CNKKSNQQNL 

       430        440        450        460        470        480 
GTIKCSNLCT EIIQYADANE VAVCNLASIA LNMFVIDGQF DFLKLKDVVK VIVRNLNKII 

       490        500        510        520        530        540 
DINYYPIPEA EISNKRHRPI GIGVQGLADA FILLNYPFDS LEAQDLNKKI FETIYYGALE 

       550        560        570        580        590        600 
ASCELAEKEG PYDTYVGSYA SNGILQYDLW NVVPSDLWNW EPLKDKIRTY GLRNSLLVAP 

       610        620        630        640        650        660 
MPTASTAQIL GNNESVEPYT SNIYTRRVLS GEFQVVNPHL LRVLTERKLW NDEIKNRIMV 

       670        680        690        700        710        720 
DGGSIQNTNL PEDIKRVYKT IWEIPQKTII KMAADRGAFI DQSQSMNIHI ADPSYSKLTS 

       730        740        750        760        770 
MHFYGWSLGL KTGMYYLRTK PASAPIQFTL DKDKIKPLVV CDSEICTSCS G 

« Hide

References

[1]"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[2]"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments."
Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S.
Virus Res. 27:25-35(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[3]"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[4]"Potential virulence determinants in terminal regions of variola smallpox virus genome."
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.
Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.
[5]Shchelkunov S.N., Chizhikov V.E., Totmenin A.V., Resenchuk S.M., Blinov V.M., Sandakhchiev L.S.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Garcia-1966.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67119 Genomic DNA. Translation: CAA47558.1.
X69198 Genomic DNA. Translation: CAA48999.1.
L22579 Genomic DNA. Translation: AAA60806.1.
X76263 Genomic DNA. Translation: CAA53832.1.
PIRB36843.
T28496.
RefSeqNP_042102.1. NC_001611.1.

3D structure databases

ProteinModelPortalP32984.
SMRP32984. Positions 15-752.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP32984.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1486459.

Phylogenomic databases

ProtClustDBCLSP2509774.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

InterProIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
SUPFAMSSF48168. SSF48168. 1 hit.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
PROSITEPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR1_VAR67
AccessionPrimary (citable) accession number: P32984
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 16, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways