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P32984

- RIR1_VAR67

UniProt

P32984 - RIR1_VAR67

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

I4L

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei202 – 2021SubstrateBy similarity
Sitei218 – 2181Important for hydrogen atom transferBy similarity
Sitei226 – 2261Allosteric effector bindingBy similarity
Binding sitei247 – 2471Substrate; via amide nitrogenBy similarity
Sitei256 – 2561Allosteric effector bindingBy similarity
Active sitei427 – 4271Proton acceptorBy similarity
Active sitei429 – 4291Cysteine radical intermediateBy similarity
Active sitei431 – 4311Proton acceptorBy similarity
Sitei444 – 4441Important for hydrogen atom transferBy similarity
Sitei735 – 7351Important for electron transferBy similarity
Sitei736 – 7361Important for electron transferBy similarity
Sitei766 – 7661Interacts with thioredoxin/glutaredoxinBy similarity
Sitei769 – 7691Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:I4L, K4L, ORF3L
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 771771Ribonucleoside-diphosphate reductase large subunitPRO_0000187233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi218 ↔ 444Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP32984.

Expressioni

Inductioni

Expressed early in the viral replicative cycle.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP32984.
SMRiP32984. Positions 15-752.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9292ATP-conePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni217 – 2182Substrate bindingBy similarity
Regioni427 – 4315Substrate bindingBy similarity
Regioni602 – 6065Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32984-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFVIKRNGYK ENVMFDKITS RIRKLCYGLN TDHIDPIKIA MKVIQGIYNG
60 70 80 90 100
VTTVELDTLT AEIAATCTTQ HPDYAILAAR IAVSNLHKET KKLFSEVMKD
110 120 130 140 150
LFNYVNPKNG KHSPIISSIT MDVVNKYKDK LNSVIIYERD FSYNYFGFKT
160 170 180 190 200
LEKSYLLKIN NKIVERPQHM LMRVAVGIHQ WDIDSAIETY NLLSEKWFTH
210 220 230 240 250
ASPTLFNAGT SRHQMSSCFL LNMMDDSIEG IYDTLKRCAL ISKMAGGIGL
260 270 280 290 300
SISNIRASGS YISGTNGASN GIIPMLRVYN NTARYIDQGG NKRPGVMTIY
310 320 330 340 350
LEPWHSDIMA FLDLKKNTGN EEHRTRDLFI ALWIPDLFMK RVKDDGEWSL
360 370 380 390 400
MCPDECPGLD NVWGDEFERL YTLYEREKRY KSIIKARVVW KAIIESQIET
410 420 430 440 450
GTPFILYKDA CNKKSNQQNL GTIKCSNLCT EIIQYADANE VAVCNLASIA
460 470 480 490 500
LNMFVIDGQF DFLKLKDVVK VIVRNLNKII DINYYPIPEA EISNKRHRPI
510 520 530 540 550
GIGVQGLADA FILLNYPFDS LEAQDLNKKI FETIYYGALE ASCELAEKEG
560 570 580 590 600
PYDTYVGSYA SNGILQYDLW NVVPSDLWNW EPLKDKIRTY GLRNSLLVAP
610 620 630 640 650
MPTASTAQIL GNNESVEPYT SNIYTRRVLS GEFQVVNPHL LRVLTERKLW
660 670 680 690 700
NDEIKNRIMV DGGSIQNTNL PEDIKRVYKT IWEIPQKTII KMAADRGAFI
710 720 730 740 750
DQSQSMNIHI ADPSYSKLTS MHFYGWSLGL KTGMYYLRTK PASAPIQFTL
760 770
DKDKIKPLVV CDSEICTSCS G
Length:771
Mass (Da):87,761
Last modified:October 1, 1993 - v1
Checksum:iE628AAA9AD95A8CD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti509 – 5091D → Y in strain: Garcia-1966.
Natural varianti764 – 7641E → K in strain: Garcia-1966.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67119 Genomic DNA. Translation: CAA47558.1.
X69198 Genomic DNA. Translation: CAA48999.1.
L22579 Genomic DNA. Translation: AAA60806.1.
X76263 Genomic DNA. Translation: CAA53832.1.
PIRiB36843.
T28496.
RefSeqiNP_042102.1. NC_001611.1.

Genome annotation databases

GeneIDi1486459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67119 Genomic DNA. Translation: CAA47558.1 .
X69198 Genomic DNA. Translation: CAA48999.1 .
L22579 Genomic DNA. Translation: AAA60806.1 .
X76263 Genomic DNA. Translation: CAA53832.1 .
PIRi B36843.
T28496.
RefSeqi NP_042102.1. NC_001611.1.

3D structure databases

ProteinModelPortali P32984.
SMRi P32984. Positions 15-752.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P32984.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1486459.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
    Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
    Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  3. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.
  5. Shchelkunov S.N., Chizhikov V.E., Totmenin A.V., Resenchuk S.M., Blinov V.M., Sandakhchiev L.S.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Garcia-1966.

Entry informationi

Entry nameiRIR1_VAR67
AccessioniPrimary (citable) accession number: P32984
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3