P32972 (TNFL8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor ligand superfamily member 8

Alternative name(s):
CD30 ligand
Short name=CD30-L
CD_antigen=CD153

Gene names

Name:	Tnfsf8
Synonyms:	Cd30l, Cd30lg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Subunit structure	Homotrimer Probable.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	immune response Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW tumor necrosis factor receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Tumor necrosis factor ligand superfamily member 8		PRO_0000185500
Regions
	Topological domain	1 – 43	43	Cytoplasmic Potential
	Transmembrane	44 – 67	24	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	68 – 239	172	Extracellular Potential
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Disulfide bond	156 ↔ 182		Potential

Sequences

	Sequence		Length	Mass (Da)
	P32972 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 29003157DD425159	FASTA	239	26,519
	10 20 30 40 50 60 MEPGLQQAGS CGAPSPDPAM QVQPGSVASP WRSTRPWRST SRSYFYLSTT ALVCLVVAVA 70 80 90 100 110 120 IILVLVVQKK DSTPNTTEKA PLKGGNCSED LFCTLKSTPS KKSWAYLQVS KHLNNTKLSW 130 140 150 160 170 180 NEDGTIHGLI YQDGNLIVQF PGLYFIVCQL QFLVQCSNHS VDLTLQLLIN SKIKKQTLVT 190 200 210 220 230 VCESGVQSKN IYQNLSQFLL HYLQVNSTIS VRVDNFQYVD TNTFPLDNVL SVFLYSSSD

References

[1]

"CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF." Smith C.A., Gruess H.-J., Davis T., Anderson D., Farrah T., Baker E., Sutherland G.R., Brannan C.I., Copeland N.G., Jenkins N.A., Grabstein K.H., Gliniak B., McAlister I.B., Fanslow W., Alderson M., Falk B., Gimpsel S., Gillis S. , Din W.S., Goodwin R.G., Armitage R.J. Cell 73:1349-1360(1993) [PubMed: 8391931] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09754 mRNA. Translation: AAA74595.1.
IPI	IPI00113840.
PIR	B40710.
RefSeq	NP_033429.1. NM_009403.3.
UniGene	Mm.4664.
3D structure databases
ProteinModelPortal	P32972.
ModBase	Search...
Protein-protein interaction databases
STRING	P32972.
Proteomic databases
PRIDE	P32972.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030047; ENSMUSP00000030047; ENSMUSG00000028362.
GeneID	21949.
KEGG	mmu:21949.
Organism-specific databases
CTD	944.
MGI	MGI:88328. Tnfsf8.
Phylogenomic databases
eggNOG	roNOG10304.
HOGENOM	HBG278304.
HOVERGEN	HBG055267.
InParanoid	P32972.
OMA	WAYLQVS.
OrthoDB	EOG42RD8M.
PhylomeDB	P32972.
Gene expression databases
ArrayExpress	P32972.
Bgee	P32972.
Genevestigator	P32972.
GermOnline	ENSMUSG00000028362. Mus musculus.
Family and domain databases
InterPro	IPR006052. TNF. IPR021185. TNF_CD30_ligand_type. IPR021184. TNF_CS. IPR008983. Tumour_necrosis_fac-like. [Graphical view]
Gene3D	G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.
KO	K05471.
Pfam	PF00229. TNF. 1 hit. [Graphical view]
PIRSF	PIRSF019548. TNF_CD30_ligand_type. 1 hit.
SMART	SM00207. TNF. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS00251. TNF_1. 1 hit. PS50049. TNF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	301608.
SOURCE	Search...

Entry information

Entry name

TNFL8_MOUSE

Accession

Primary (citable) accession number: P32972

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program