ID   TNFL8_HUMAN             Reviewed;         234 AA.
AC   P32971; O43404;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   27-MAR-2024, entry version 165.
DE   RecName: Full=Tumor necrosis factor ligand superfamily member 8;
DE   AltName: Full=CD30 ligand;
DE            Short=CD30-L;
DE   AltName: CD_antigen=CD153;
GN   Name=TNFSF8; Synonyms=CD30L, CD30LG;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8391931; DOI=10.1016/0092-8674(93)90361-s;
RA   Smith C.A., Gruess H.-J., Davis T., Anderson D., Farrah T., Baker E.,
RA   Sutherland G.R., Brannan C.I., Copeland N.G., Jenkins N.A., Grabstein K.H.,
RA   Gliniak B., McAlister I.B., Fanslow W., Alderson M., Falk B., Gimpsel S.,
RA   Gillis S., Din W.S., Goodwin R.G., Armitage R.J.;
RT   "CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand
RT   defines an emerging family of cytokines with homology to TNF.";
RL   Cell 73:1349-1360(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9349718; DOI=10.1016/s0167-4781(97)00108-5;
RA   Croager E.J., Abraham L.J.;
RT   "Characterisation of the human CD30 ligand gene structure.";
RL   Biochim. Biophys. Acta 1353:231-235(1997).
CC   -!- FUNCTION: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of
CC       T-cells.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000305}.
CC   -!- INTERACTION:
CC       P32971; O95393: BMP10; NbExp=3; IntAct=EBI-13076860, EBI-3922513;
CC       P32971; Q07325: CXCL9; NbExp=3; IntAct=EBI-13076860, EBI-3911467;
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
CC   -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L09753; AAA74594.1; -; mRNA.
DR   EMBL; AF006384; AAB97877.1; -; Genomic_DNA.
DR   EMBL; AF006381; AAB97877.1; JOINED; Genomic_DNA.
DR   EMBL; AF006382; AAB97877.1; JOINED; Genomic_DNA.
DR   EMBL; AF006383; AAB97877.1; JOINED; Genomic_DNA.
DR   CCDS; CCDS6810.1; -.
DR   PIR; A40710; A40710.
DR   RefSeq; NP_001235.1; NM_001244.3.
DR   AlphaFoldDB; P32971; -.
DR   SMR; P32971; -.
DR   BioGRID; 107382; 170.
DR   DIP; DIP-2929N; -.
DR   IntAct; P32971; 38.
DR   STRING; 9606.ENSP00000223795; -.
DR   GlyCosmos; P32971; 5 sites, No reported glycans.
DR   GlyGen; P32971; 5 sites.
DR   iPTMnet; P32971; -.
DR   PhosphoSitePlus; P32971; -.
DR   BioMuta; TNFSF8; -.
DR   DMDM; 416777; -.
DR   MassIVE; P32971; -.
DR   PaxDb; 9606-ENSP00000223795; -.
DR   PeptideAtlas; P32971; -.
DR   ProteomicsDB; 54895; -.
DR   Antibodypedia; 15592; 514 antibodies from 31 providers.
DR   DNASU; 944; -.
DR   Ensembl; ENST00000223795.3; ENSP00000223795.2; ENSG00000106952.8.
DR   GeneID; 944; -.
DR   KEGG; hsa:944; -.
DR   MANE-Select; ENST00000223795.3; ENSP00000223795.2; NM_001244.4; NP_001235.1.
DR   AGR; HGNC:11938; -.
DR   CTD; 944; -.
DR   DisGeNET; 944; -.
DR   GeneCards; TNFSF8; -.
DR   HGNC; HGNC:11938; TNFSF8.
DR   HPA; ENSG00000106952; Tissue enhanced (bone marrow, lymphoid tissue).
DR   MIM; 603875; gene.
DR   neXtProt; NX_P32971; -.
DR   OpenTargets; ENSG00000106952; -.
DR   PharmGKB; PA36628; -.
DR   VEuPathDB; HostDB:ENSG00000106952; -.
DR   eggNOG; ENOG502S54A; Eukaryota.
DR   GeneTree; ENSGT00390000011719; -.
DR   HOGENOM; CLU_102229_0_0_1; -.
DR   InParanoid; P32971; -.
DR   OMA; VNKFQYV; -.
DR   OrthoDB; 4584718at2759; -.
DR   PhylomeDB; P32971; -.
DR   TreeFam; TF335780; -.
DR   PathwayCommons; P32971; -.
DR   Reactome; R-HSA-5669034; TNFs bind their physiological receptors.
DR   SignaLink; P32971; -.
DR   BioGRID-ORCS; 944; 6 hits in 1144 CRISPR screens.
DR   ChiTaRS; TNFSF8; human.
DR   GenomeRNAi; 944; -.
DR   Pharos; P32971; Tbio.
DR   PRO; PR:P32971; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   RNAct; P32971; Protein.
DR   Bgee; ENSG00000106952; Expressed in monocyte and 112 other cell types or tissues.
DR   ExpressionAtlas; P32971; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005102; F:signaling receptor binding; TAS:ProtInc.
DR   GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR   GO; GO:0043374; P:CD8-positive, alpha-beta T cell differentiation; IBA:GO_Central.
DR   GO; GO:0007267; P:cell-cell signaling; TAS:ProtInc.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:Ensembl.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0042129; P:regulation of T cell proliferation; TAS:GO_Central.
DR   CDD; cd00184; TNF; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR021185; TNF_CD30_ligand_type.
DR   InterPro; IPR021184; TNF_CS.
DR   InterPro; IPR006052; TNF_dom.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   PANTHER; PTHR32163; TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 8; 1.
DR   PANTHER; PTHR32163:SF1; TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 8; 1.
DR   Pfam; PF00229; TNF; 1.
DR   PIRSF; PIRSF019548; TNF_CD30_ligand_type; 1.
DR   SMART; SM00207; TNF; 1.
DR   SUPFAM; SSF49842; TNF-like; 1.
DR   PROSITE; PS00251; TNF_1; 1.
DR   PROSITE; PS50049; TNF_2; 1.
DR   Genevisible; P32971; HS.
PE   1: Evidence at protein level;
KW   Cytokine; Disulfide bond; Glycoprotein; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..234
FT                   /note="Tumor necrosis factor ligand superfamily member 8"
FT                   /id="PRO_0000185499"
FT   TOPO_DOM        1..37
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..62
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..234
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        81
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        189
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        201
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        151..177
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   234 AA;  26017 MW;  C653615682305B1B CRC64;
     MDPGLQQALN GMAPPGDTAM HVPAGSVASH LGTTSRSYFY LTTATLALCL VFTVATIMVL
     VVQRTDSIPN SPDNVPLKGG NCSEDLLCIL KRAPFKKSWA YLQVAKHLNK TKLSWNKDGI
     LHGVRYQDGN LVIQFPGLYF IICQLQFLVQ CPNNSVDLKL ELLINKHIKK QALVTVCESG
     MQTKHVYQNL SQFLLDYLQV NTTISVNVDT FQYIDTSTFP LENVLSIFLY SNSD
//