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Protein

Nitrilase 2

Gene

NIT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.2 Publications

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei58Proton acceptorPROSITE-ProRule annotation1
Active sitei145Proton donorPROSITE-ProRule annotation1
Active sitei179NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • indole-3-acetonitrile nitrilase activity Source: TAIR
  • indole-3-acetonitrile nitrile hydratase activity Source: TAIR
  • nitrilase activity Source: TAIR

GO - Biological processi

  • indoleacetic acid biosynthetic process Source: TAIR
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciARA:AT3G44300-MONOMER.
MetaCyc:AT3G44300-MONOMER.
BRENDAi3.5.5.1. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase 2 (EC:3.5.5.1)
Gene namesi
Name:NIT2
Ordered Locus Names:At3g44300
ORF Names:T10D17_90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G44300.
TAIRilocus:2095735. AT3G44300.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002040372 – 339Nitrilase 2Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP32962.
PRIDEiP32962.

PTM databases

iPTMnetiP32962.

Expressioni

Developmental stagei

Barely detectable in young rosettes, but is strongly expressed during bolting, flowering, and especially fruit development.

Inductioni

By indole-3-acetonitrile, abscisic acid (ABA), salicylic acid (SA), sodium nitroprusside (SNP), salt stress and dehydration stress.1 Publication

Gene expression databases

ExpressionAtlasiP32962. baseline and differential.
GenevisibleiP32962. AT.

Interactioni

Protein-protein interaction databases

BioGridi8875. 1 interactor.
STRINGi3702.AT3G44300.1.

Structurei

3D structure databases

ProteinModelPortaliP32962.
SMRiP32962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 290CN hydrolasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
HOGENOMiHOG000256365.
InParanoidiP32962.
KOiK01501.
OMAiNVIGFPE.
OrthoDBiEOG09360CLO.
PhylomeDBiP32962.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSENTPFN GVASSTIVRA TIVQASTVYN DTPATLEKAN KFIVEAASKG
60 70 80 90 100
SELVVFPEAF IGGYPRGFRF GLGVGVHNEE GRDEFRKYHA SAIKVPGPEV
110 120 130 140 150
EKLAELAGKN NVYLVMGAIE KDGYTLYCTA LFFSPQGQFL GKHRKLMPTS
160 170 180 190 200
LERCIWGQGD GSTIPVYDTP IGKLGAAICW ENRMPLYRTA LYAKGIELYC
210 220 230 240 250
APTADGSKEW QSSMLHIAIE GGCFVLSACQ FCLRKDFPDH PDYLFTDWYD
260 270 280 290 300
DKEPDSIVSQ GGSVIISPLG QVLAGPNFES EGLITADLDL GDVARAKLYF
310 320 330
DSVGHYSRPD VLHLTVNEHP KKPVTFISKV EKAEDDSNK
Length:339
Mass (Da):37,153
Last modified:October 1, 1993 - v1
Checksum:i06CDE76D2FDC24A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37E → G in AAB05220 (Ref. 3) Curated1
Sequence conflicti48S → T in AAB05220 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68305 mRNA. Translation: CAA48377.1.
U09958 mRNA. Translation: AAB60275.1.
U38845 Genomic DNA. Translation: AAB05220.1.
AL353865 Genomic DNA. Translation: CAB88998.1.
CP002686 Genomic DNA. Translation: AEE77886.1.
AY088028 mRNA. Translation: AAM65574.1.
PIRiS31969.
T52262.
RefSeqiNP_190016.1. NM_114298.3.
UniGeneiAt.5404.
At.67732.

Genome annotation databases

EnsemblPlantsiAT3G44300.1; AT3G44300.1; AT3G44300.
GeneIDi823555.
GrameneiAT3G44300.1; AT3G44300.1; AT3G44300.
KEGGiath:AT3G44300.

Similar proteinsi

Entry informationi

Entry nameiNRL2_ARATH
AccessioniPrimary (citable) accession number: P32962
Secondary accession number(s): Q96505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 25, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families