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P32961

- NRL1_ARATH

UniProt

P32961 - NRL1_ARATH

Protein

Nitrilase 1

Gene

NIT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.

    Catalytic activityi

    A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Proton acceptorPROSITE-ProRule annotation
    Active sitei152 – 1521Proton donorPROSITE-ProRule annotation
    Active sitei186 – 1861NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. indole-3-acetonitrile nitrilase activity Source: TAIR
    2. indole-3-acetonitrile nitrile hydratase activity Source: TAIR
    3. nitrilase activity Source: TAIR

    GO - Biological processi

    1. indoleacetic acid biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT3G44310-MONOMER.
    ARA:GQT-941-MONOMER.
    ARA:GQT-942-MONOMER.
    MetaCyc:AT3G44310-MONOMER.
    BRENDAi3.5.5.1. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrilase 1 (EC:3.5.5.1)
    Gene namesi
    Name:NIT1
    Ordered Locus Names:At3g44310
    ORF Names:T10D17_100
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G44310.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. cytosol Source: TAIR
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 346345Nitrilase 1PRO_0000204036Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PaxDbiP32961.
    PRIDEiP32961.

    Expressioni

    Tissue specificityi

    Expressed in cotyledons, hypocotyls, leaves, roots, stems, flowers and siliques.1 Publication

    Developmental stagei

    Expressed throughout development, but at a very low level during the fruiting stage.

    Gene expression databases

    GenevestigatoriP32961.

    Interactioni

    Protein-protein interaction databases

    BioGridi8876. 2 interactions.
    IntActiP32961. 1 interaction.
    STRINGi3702.AT3G44310.3-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP32961.
    SMRiP32961. Positions 25-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 320296CN hydrolasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0388.
    HOGENOMiHOG000256365.
    InParanoidiP32961.
    KOiK01501.
    OMAiEGREWIN.
    PhylomeDBiP32961.

    Family and domain databases

    Gene3Di3.60.110.10. 1 hit.
    InterProiIPR003010. C-N_Hydrolase.
    IPR000132. Nitrilase/CN_hydratase_CS.
    [Graphical view]
    PfamiPF00795. CN_hydrolase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56317. SSF56317. 1 hit.
    PROSITEiPS50263. CN_HYDROLASE. 1 hit.
    PS00920. NITRIL_CHT_1. 1 hit.
    PS00921. NITRIL_CHT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P32961-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSTKDMSTV QNATPFNGVA PSTTVRVTIV QSSTVYNDTP ATIDKAEKYI    50
    VEAASKGAEL VLFPEGFIGG YPRGFRFGLA VGVHNEEGRD EFRKYHASAI 100
    HVPGPEVARL ADVARKNHVY LVMGAIEKEG YTLYCTVLFF SPQGQFLGKH 150
    RKLMPTSLER CIWGQGDGST IPVYDTPIGK LGAAICWENR MPLYRTALYA 200
    KGIELYCAPT ADGSKEWQSS MLHIAIEGGC FVLSACQFCQ RKHFPDHPDY 250
    LFTDWYDDKE HDSIVSQGGS VIISPLGQVL AGPNFESEGL VTADIDLGDI 300
    ARAKLYFDSV GHYSRPDVLH LTVNEHPRKS VTFVTKVEKA EDDSNK 346
    Length:346
    Mass (Da):38,152
    Last modified:February 5, 2008 - v2
    Checksum:i8D4F93661CAE3C1F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti312 – 3121H → Y in CAA45041. (PubMed:1555601)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63445 mRNA. Translation: CAA45041.1.
    U38845 Genomic DNA. Translation: AAB05221.1.
    Y07648 Genomic DNA. Translation: CAA68935.2.
    AL353865 Genomic DNA. Translation: CAB88999.1.
    CP002686 Genomic DNA. Translation: AEE77887.1.
    CP002686 Genomic DNA. Translation: AEE77889.1.
    AY042847 mRNA. Translation: AAK68787.1.
    BT000040 mRNA. Translation: AAN15359.1.
    DQ446730 mRNA. Translation: ABE65989.1.
    PIRiS22398.
    T49147.
    RefSeqiNP_001078234.1. NM_001084765.1. [P32961-1]
    NP_851011.1. NM_180680.2. [P32961-1]
    UniGeneiAt.23699.
    At.23715.
    At.5403.
    At.5404.

    Genome annotation databases

    EnsemblPlantsiAT3G44310.1; AT3G44310.1; AT3G44310. [P32961-1]
    AT3G44310.3; AT3G44310.3; AT3G44310. [P32961-1]
    GeneIDi823556.
    KEGGiath:AT3G44310.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63445 mRNA. Translation: CAA45041.1 .
    U38845 Genomic DNA. Translation: AAB05221.1 .
    Y07648 Genomic DNA. Translation: CAA68935.2 .
    AL353865 Genomic DNA. Translation: CAB88999.1 .
    CP002686 Genomic DNA. Translation: AEE77887.1 .
    CP002686 Genomic DNA. Translation: AEE77889.1 .
    AY042847 mRNA. Translation: AAK68787.1 .
    BT000040 mRNA. Translation: AAN15359.1 .
    DQ446730 mRNA. Translation: ABE65989.1 .
    PIRi S22398.
    T49147.
    RefSeqi NP_001078234.1. NM_001084765.1. [P32961-1 ]
    NP_851011.1. NM_180680.2. [P32961-1 ]
    UniGenei At.23699.
    At.23715.
    At.5403.
    At.5404.

    3D structure databases

    ProteinModelPortali P32961.
    SMRi P32961. Positions 25-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 8876. 2 interactions.
    IntActi P32961. 1 interaction.
    STRINGi 3702.AT3G44310.3-P.

    Proteomic databases

    PaxDbi P32961.
    PRIDEi P32961.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G44310.1 ; AT3G44310.1 ; AT3G44310 . [P32961-1 ]
    AT3G44310.3 ; AT3G44310.3 ; AT3G44310 . [P32961-1 ]
    GeneIDi 823556.
    KEGGi ath:AT3G44310.

    Organism-specific databases

    TAIRi AT3G44310.

    Phylogenomic databases

    eggNOGi COG0388.
    HOGENOMi HOG000256365.
    InParanoidi P32961.
    KOi K01501.
    OMAi EGREWIN.
    PhylomeDBi P32961.

    Enzyme and pathway databases

    BioCyci ARA:AT3G44310-MONOMER.
    ARA:GQT-941-MONOMER.
    ARA:GQT-942-MONOMER.
    MetaCyc:AT3G44310-MONOMER.
    BRENDAi 3.5.5.1. 399.

    Gene expression databases

    Genevestigatori P32961.

    Family and domain databases

    Gene3Di 3.60.110.10. 1 hit.
    InterProi IPR003010. C-N_Hydrolase.
    IPR000132. Nitrilase/CN_hydratase_CS.
    [Graphical view ]
    Pfami PF00795. CN_hydrolase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56317. SSF56317. 1 hit.
    PROSITEi PS50263. CN_HYDROLASE. 1 hit.
    PS00920. NITRIL_CHT_1. 1 hit.
    PS00921. NITRIL_CHT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of an Arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone, indole-3-acetic acid."
      Bartling D., Seedorf M., Mithoefer A., Weiler E.W.
      Eur. J. Biochem. 205:417-424(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: cv. Landsberg erecta.
      Tissue: Leaf.
    2. "Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana."
      Bartel B., Fink G.R.
      Proc. Natl. Acad. Sci. U.S.A. 91:6649-6653(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    3. "Nucleotide sequence of the Arabidopsis thaliana nitrilase 1 gene."
      Zhou L., Bartel B., Thornburg R.W.
      Plant Gene Register PGR95-130
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    4. "Structural analysis of the nit2/nit1/nit3 gene cluster encoding nitrilases, enzymes catalyzing the terminal activation step in indole-acetic acid biosynthesis in Arabidopsis thaliana."
      Hillebrand H., Bartling D., Weiler E.W.
      Plant Mol. Biol. 36:89-99(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    6. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    8. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
      Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
      Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    9. "Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: key enzymes in biosynthesis of the plant hormone indole-3-acetic acid."
      Bartling D., Seedorf M., Schmidt R.C., Weiler E.W.
      Proc. Natl. Acad. Sci. U.S.A. 91:6021-6025(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: cv. Landsberg erecta.
    10. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNRL1_ARATH
    AccessioniPrimary (citable) accession number: P32961
    Secondary accession number(s): O04908
    , Q42543, Q53YI1, Q94B53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3