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P32961 (NRL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrilase 1

EC=3.5.5.1
Gene names
Name:NIT1
Ordered Locus Names:At3g44310
ORF Names:T10D17_100
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.

Catalytic activity

A nitrile + 2 H2O = a carboxylate + NH3.

Tissue specificity

Expressed in cotyledons, hypocotyls, leaves, roots, stems, flowers and siliques. Ref.2

Developmental stage

Expressed throughout development, but at a very low level during the fruiting stage.

Post-translational modification

Ubiquitinated.

Sequence similarities

Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.

Contains 1 CN hydrolase domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P32961-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 346345Nitrilase 1
PRO_0000204036

Regions

Domain25 – 320296CN hydrolase

Sites

Active site651Proton acceptor By similarity
Active site1521Proton donor By similarity
Active site1861Nucleophile By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity

Experimental info

Sequence conflict3121H → Y in CAA45041. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 8D4F93661CAE3C1F

FASTA34638,152
        10         20         30         40         50         60 
MSSTKDMSTV QNATPFNGVA PSTTVRVTIV QSSTVYNDTP ATIDKAEKYI VEAASKGAEL 

        70         80         90        100        110        120 
VLFPEGFIGG YPRGFRFGLA VGVHNEEGRD EFRKYHASAI HVPGPEVARL ADVARKNHVY 

       130        140        150        160        170        180 
LVMGAIEKEG YTLYCTVLFF SPQGQFLGKH RKLMPTSLER CIWGQGDGST IPVYDTPIGK 

       190        200        210        220        230        240 
LGAAICWENR MPLYRTALYA KGIELYCAPT ADGSKEWQSS MLHIAIEGGC FVLSACQFCQ 

       250        260        270        280        290        300 
RKHFPDHPDY LFTDWYDDKE HDSIVSQGGS VIISPLGQVL AGPNFESEGL VTADIDLGDI 

       310        320        330        340 
ARAKLYFDSV GHYSRPDVLH LTVNEHPRKS VTFVTKVEKA EDDSNK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of an Arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone, indole-3-acetic acid."
Bartling D., Seedorf M., Mithoefer A., Weiler E.W.
Eur. J. Biochem. 205:417-424(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: cv. Landsberg erecta.
Tissue: Leaf.
[2]"Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana."
Bartel B., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 91:6649-6653(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[3]"Nucleotide sequence of the Arabidopsis thaliana nitrilase 1 gene."
Zhou L., Bartel B., Thornburg R.W.
Plant Gene Register PGR95-130
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[4]"Structural analysis of the nit2/nit1/nit3 gene cluster encoding nitrilases, enzymes catalyzing the terminal activation step in indole-acetic acid biosynthesis in Arabidopsis thaliana."
Hillebrand H., Bartling D., Weiler E.W.
Plant Mol. Biol. 36:89-99(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[9]"Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: key enzymes in biosynthesis of the plant hormone indole-3-acetic acid."
Bartling D., Seedorf M., Schmidt R.C., Weiler E.W.
Proc. Natl. Acad. Sci. U.S.A. 91:6021-6025(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: cv. Landsberg erecta.
[10]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63445 mRNA. Translation: CAA45041.1.
U38845 Genomic DNA. Translation: AAB05221.1.
Y07648 Genomic DNA. Translation: CAA68935.2.
AL353865 Genomic DNA. Translation: CAB88999.1.
CP002686 Genomic DNA. Translation: AEE77887.1.
CP002686 Genomic DNA. Translation: AEE77889.1.
AY042847 mRNA. Translation: AAK68787.1.
BT000040 mRNA. Translation: AAN15359.1.
DQ446730 mRNA. Translation: ABE65989.1.
PIRS22398.
T49147.
RefSeqNP_001078234.1. NM_001084765.1. [P32961-1]
NP_851011.1. NM_180680.2. [P32961-1]
UniGeneAt.23699.
At.23715.
At.5403.
At.5404.

3D structure databases

ProteinModelPortalP32961.
SMRP32961. Positions 25-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid8876. 2 interactions.
IntActP32961. 1 interaction.
STRING3702.AT3G44310.3-P.

Proteomic databases

PaxDbP32961.
PRIDEP32961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G44310.1; AT3G44310.1; AT3G44310. [P32961-1]
AT3G44310.3; AT3G44310.3; AT3G44310. [P32961-1]
GeneID823556.
KEGGath:AT3G44310.

Organism-specific databases

TAIRAT3G44310.

Phylogenomic databases

eggNOGCOG0388.
HOGENOMHOG000256365.
InParanoidP32961.
KOK01501.
OMAEGREWIN.
PhylomeDBP32961.

Enzyme and pathway databases

BioCycARA:AT3G44310-MONOMER.
ARA:GQT-941-MONOMER.
ARA:GQT-942-MONOMER.
MetaCyc:AT3G44310-MONOMER.
BRENDA3.5.5.1. 399.

Gene expression databases

GenevestigatorP32961.

Family and domain databases

Gene3D3.60.110.10. 1 hit.
InterProIPR003010. C-N_Hydrolase.
IPR000132. Nitrilase/CN_hydratase_CS.
[Graphical view]
PfamPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMSSF56317. SSF56317. 1 hit.
PROSITEPS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNRL1_ARATH
AccessionPrimary (citable) accession number: P32961
Secondary accession number(s): O04908 expand/collapse secondary AC list , Q42543, Q53YI1, Q94B53
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 5, 2008
Last modified: June 11, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names