Reviewed, UniProtKB/Swiss-Prot P32949 (LIP5_CANRU)

Names and origin

Protein names

Recommended name:
    Lipase 5
    EC=3.1.1.3

Gene names

Name:

LIP5

Organism

Candida rugosa (Yeast) (Candida cylindracea)

Taxonomic identifier

5481 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Chain	16 – 549	534	Lipase 5		PRO_0000008623
Sites
	Active site	224	1	Acyl-ester intermediate By similarity
	Active site	356	1	Charge relay system By similarity
	Active site	464	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	329	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Disulfide bond	75 ↔ 112		By similarity
	Disulfide bond	283 ↔ 292		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32949-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 0F3D04C9716F6F22	FASTA	549	58,420
	10 20 30 40 50 60 MKLALALSLI ASVAAAPTAT LANGDTITGL NAIINEAFLG IPFAEPPVGN LRFKDPVPYR 70 80 90 100 110 120 GSLNGQSFTA YGPSCMQQNP EGTYEENLPK VALDLVMQSK VFQAVLPNSE DCLTINVVRP 130 140 150 160 170 180 PGTKAGANLP VMLWIFGGGF EIGSPTIFPP AQMVSKSVLM GKPIIHVAVN YRLASFGFLA 190 200 210 220 230 240 GPDIKAEGSS NAGLKDQRLG MQWVADNIAG FGGDPSKVTI FGESAGSMSV LCHLLWNGGD 250 260 270 280 290 300 NTYKGKPLFR AGIMQSGAMV PSDPVDGTYG TQIYDTLVAS TGCSSASNKL ACLRGLSTQA 310 320 330 340 350 360 LLDATNDTPG FLSYTSLRLS YLPRPDGANI TDDMYKLVRD GKYASVPVII GDQNDEGFLF 370 380 390 400 410 420 GLSSLNTTTE ADAEAYLRKS FIHATDADIT ALKAAYPSDV TQGSPFDTGI LNALTPQLKR 430 440 450 460 470 480 INAVLGDLTF TLSRRYFLNH YTGGPKYSFL SKQLSGLPIL GTFHANDIVW QHFLLGSGSV 490 500 510 520 530 540 IYNNAFIAFA TDLDPNTAGL SVQWPKSTSS SQAGDNLMQI SALGLYTGKD NFRTAGYNAL FADPSHFFV

References

[1]	"Cloning and analysis of Candida cylindracea lipase sequences." Lotti M., Grandori R., Fusetti F., Longhi S., Brocca S., Tramontano A., Alberghina L. Gene 124:45-55(1993) [PubMed: 8440480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14830 / CBS 6330 / DSM 2031 / MS-5 / NRRL Y-17506.
[2]	"Candida rugosa lipases: molecular biology and versatility in biotechnology." Benjamin S., Pandey A. Yeast 14:1069-1087(1998) [PubMed: 9778794] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66008 Genomic DNA. Translation: CAA46807.1.
PIR	JN0553.
3D structure databases
HSSP	HSSP built from PDB template 1LLF based on UniProtKB P32947.
SMR	P32949. Positions 16-549.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.3. 3604.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. [Graphical view]
PANTHER	PTHR11559. CarbesteraseB. 1 hit.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP5_CANRU

Accession

Primary (citable) accession number: P32949

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)