P32948 (LIP4_CANRU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipase 4
EC=3.1.1.3

Gene names

Name:

LIP4

Organism

Candida rugosa (Yeast) (Candida cylindracea)

Taxonomic identifier

5481 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Chain	16 – 549	534	Lipase 4		PRO_0000008622
Sites
	Active site	224	1	Acyl-ester intermediate By similarity
	Active site	356	1	Charge relay system By similarity
	Active site	464	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Disulfide bond	75 ↔ 112		By similarity
	Disulfide bond	283 ↔ 292		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32948 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 39C7160852F7E655	FASTA	549	58,571
	10 20 30 40 50 60 MKLALVLSLI VSVAAAPTAT LANGDTITGL NAIINEAFLG IPFAQPPVGN LRFKPPVPYS 70 80 90 100 110 120 ASLNGQKFTS YGPSCMQMNP LGNWDSSLPK AAINSLMQSK LFQAVLPNGE DCLTINVVRP 130 140 150 160 170 180 SGTKPGANLP VMVWIFGGGF EVGGSSLFPP AQMITASVLM GKPIIHVSMN YRVASWGFLA 190 200 210 220 230 240 GPDIKAEGSG NAGLHDQRLG LQWVADNIAG FGGDPSKVTI FGESAGSMSV MCQLLWNDGD 250 260 270 280 290 300 NTYNGKPLFR AAIMQSGAMV PSDPVDGPYG TQIYDQVVAS AGCGSASDKL ACLRSISNDK 310 320 330 340 350 360 LFQATSDTPG ALAYPSLRLS FLPRPDGTFI TDDMFKLVRD GKCANVPVII GDQNDEGTVF 370 380 390 400 410 420 ALSSLNVTTD AQARQYFKES FIHASDAEID TLMAAYPSDI TQGSPFDTGI FNAITPQFKR 430 440 450 460 470 480 IAAVLGDLAF TLPRRYFLNH FQGGTKYSFL SKQLSGLPVI GTHHANDIVW QDFLVSHSSA 490 500 510 520 530 540 VYNNAFIAFA NDLDPNKAGL LVNWPKYTSS SQSGNNLLQI NALGLYTGKD NFRTAGYDAL FTNPSSFFV

References

[1]	"Cloning and analysis of Candida cylindracea lipase sequences." Lotti M., Grandori R., Fusetti F., Longhi S., Brocca S., Tramontano A., Alberghina L. Gene 124:45-55(1993) [PubMed: 8440480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14830 / CBS 6330 / DSM 2031 / MS-5 / NRRL Y-17506.
[2]	"Candida rugosa lipases: molecular biology and versatility in biotechnology." Benjamin S., Pandey A. Yeast 14:1069-1087(1998) [PubMed: 9778794] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66007 Genomic DNA. Translation: CAA46806.1.
PIR	JN0552.
3D structure databases
ProteinModelPortal	P32948.
SMR	P32948. Positions 16-549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP4_CANRU

Accession

Primary (citable) accession number: P32948

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 31, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program