Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipase 3

Gene

LIP3

Organism
Candida rugosa (Yeast) (Candida cylindracea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei224 – 2241Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei356 – 3561Charge relay systemBy similarity
Active sitei464 – 4641Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

BRENDAi3.1.1.3. 1139.

Protein family/group databases

ESTHERicanru-3lipa. Fungal_carboxylesterase_lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 3 (EC:3.1.1.3)
Alternative name(s):
Cholesterol esterase
Gene namesi
Name:LIP3
OrganismiCandida rugosa (Yeast) (Candida cylindracea)
Taxonomic identifieri5481 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15151 PublicationAdd
BLAST
Chaini16 – 549534Lipase 3PRO_0000008621Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 112By similarity
Disulfide bondi283 ↔ 292By similarity
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence analysis
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

Structurei

Secondary structure

1
549
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 203Combined sources
Beta strandi26 – 283Combined sources
Beta strandi36 – 427Combined sources
Helixi49 – 513Combined sources
Helixi88 – 9811Combined sources
Helixi100 – 1056Combined sources
Beta strandi114 – 1196Combined sources
Beta strandi129 – 1357Combined sources
Turni139 – 1413Combined sources
Helixi145 – 1473Combined sources
Helixi151 – 1599Combined sources
Beta strandi165 – 1695Combined sources
Helixi174 – 1785Combined sources
Helixi182 – 1876Combined sources
Helixi192 – 20716Combined sources
Helixi208 – 2114Combined sources
Beta strandi213 – 22311Combined sources
Helixi225 – 23511Combined sources
Helixi236 – 2394Combined sources
Beta strandi246 – 2483Combined sources
Beta strandi250 – 2567Combined sources
Helixi268 – 28114Combined sources
Helixi289 – 2946Combined sources
Helixi298 – 3058Combined sources
Turni314 – 3174Combined sources
Beta strandi327 – 3304Combined sources
Helixi334 – 3396Combined sources
Beta strandi348 – 3536Combined sources
Helixi356 – 3583Combined sources
Helixi360 – 3645Combined sources
Helixi370 – 38011Combined sources
Helixi386 – 39510Combined sources
Turni400 – 4023Combined sources
Beta strandi403 – 4053Combined sources
Turni409 – 4124Combined sources
Beta strandi413 – 4175Combined sources
Helixi418 – 42912Combined sources
Helixi431 – 44010Combined sources
Beta strandi446 – 4516Combined sources
Turni453 – 4564Combined sources
Turni458 – 4603Combined sources
Beta strandi461 – 4633Combined sources
Helixi466 – 4727Combined sources
Helixi479 – 4824Combined sources
Helixi484 – 4929Combined sources
Helixi495 – 4984Combined sources
Beta strandi517 – 5204Combined sources
Beta strandi525 – 5284Combined sources
Helixi534 – 5418Combined sources
Helixi544 – 5474Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CLEX-ray2.00A/B16-549[»]
1LLFX-ray1.40A/B16-549[»]
ProteinModelPortaliP32947.
SMRiP32947. Positions 16-549.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32947.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLALALSLI ASVAAAPTAK LANGDTITGL NAIINEAFLG IPFAEPPVGN
60 70 80 90 100
LRFKDPVPYS GSLNGQKFTS YGPSCMQQNP EGTFEENLGK TALDLVMQSK
110 120 130 140 150
VFQAVLPQSE DCLTINVVRP PGTKAGANLP VMLWIFGGGF EIGSPTIFPP
160 170 180 190 200
AQMVTKSVLM GKPIIHVAVN YRVASWGFLA GDDIKAEGSG NAGLKDQRLG
210 220 230 240 250
MQWVADNIAG FGGDPSKVTI FGESAGSMSV LCHLIWNDGD NTYKGKPLFR
260 270 280 290 300
AGIMQSGAMV PSDPVDGTYG NEIYDLFVSS AGCGSASDKL ACLRSASSDT
310 320 330 340 350
LLDATNNTPG FLAYSSLRLS YLPRPDGKNI TDDMYKLVRD GKYASVPVII
360 370 380 390 400
GDQNDEGTIF GLSSLNVTTN AQARAYFKQS FIHASDAEID TLMAAYPQDI
410 420 430 440 450
TQGSPFDTGI FNAITPQFKR ISAVLGDLAF IHARRYFLNH FQGGTKYSFL
460 470 480 490 500
SKQLSGLPIM GTFHANDIVW QDYLLGSGSV IYNNAFIAFA TDLDPNTAGL
510 520 530 540
LVNWPKYTSS SQSGNNLMMI NALGLYTGKD NFRTAGYDAL MTNPSSFFV
Length:549
Mass (Da):58,755
Last modified:October 1, 1993 - v1
Checksum:iC5F95C31422975DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66006 Genomic DNA. Translation: CAA46805.1.
PIRiJN0551.
S41735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66006 Genomic DNA. Translation: CAA46805.1.
PIRiJN0551.
S41735.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CLEX-ray2.00A/B16-549[»]
1LLFX-ray1.40A/B16-549[»]
ProteinModelPortaliP32947.
SMRiP32947. Positions 16-549.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERicanru-3lipa. Fungal_carboxylesterase_lipase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.1.3. 1139.

Miscellaneous databases

EvolutionaryTraceiP32947.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIP3_CANRU
AccessioniPrimary (citable) accession number: P32947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 14, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.