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Protein

Lipase 3

Gene

LIP3

Organism
Candida rugosa (Yeast) (Candida cylindracea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei224Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei356Charge relay systemBy similarity1
Active sitei464Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

BRENDAi3.1.1.3. 1139.

Protein family/group databases

ESTHERicanru-3lipa. Fungal_carboxylesterase_lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 3 (EC:3.1.1.3)
Alternative name(s):
Cholesterol esterase
Gene namesi
Name:LIP3
OrganismiCandida rugosa (Yeast) (Candida cylindracea)
Taxonomic identifieri5481 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetales incertae sedisDiutina

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000862116 – 549Lipase 3Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 112By similarity
Disulfide bondi283 ↔ 292By similarity
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Glycosylationi366N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

Structurei

Secondary structure

1549
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 20Combined sources3
Beta strandi26 – 28Combined sources3
Beta strandi36 – 42Combined sources7
Helixi49 – 51Combined sources3
Helixi88 – 98Combined sources11
Helixi100 – 105Combined sources6
Beta strandi114 – 119Combined sources6
Beta strandi129 – 135Combined sources7
Turni139 – 141Combined sources3
Helixi145 – 147Combined sources3
Helixi151 – 159Combined sources9
Beta strandi165 – 169Combined sources5
Helixi174 – 178Combined sources5
Helixi182 – 187Combined sources6
Helixi192 – 207Combined sources16
Helixi208 – 211Combined sources4
Beta strandi213 – 223Combined sources11
Helixi225 – 235Combined sources11
Helixi236 – 239Combined sources4
Beta strandi246 – 248Combined sources3
Beta strandi250 – 256Combined sources7
Helixi268 – 281Combined sources14
Helixi289 – 294Combined sources6
Helixi298 – 305Combined sources8
Turni314 – 317Combined sources4
Beta strandi327 – 330Combined sources4
Helixi334 – 339Combined sources6
Beta strandi348 – 353Combined sources6
Helixi356 – 358Combined sources3
Helixi360 – 364Combined sources5
Helixi370 – 380Combined sources11
Helixi386 – 395Combined sources10
Turni400 – 402Combined sources3
Beta strandi403 – 405Combined sources3
Turni409 – 412Combined sources4
Beta strandi413 – 417Combined sources5
Helixi418 – 429Combined sources12
Helixi431 – 440Combined sources10
Beta strandi446 – 451Combined sources6
Turni453 – 456Combined sources4
Turni458 – 460Combined sources3
Beta strandi461 – 463Combined sources3
Helixi466 – 472Combined sources7
Helixi479 – 482Combined sources4
Helixi484 – 492Combined sources9
Helixi495 – 498Combined sources4
Beta strandi517 – 520Combined sources4
Beta strandi525 – 528Combined sources4
Helixi534 – 541Combined sources8
Helixi544 – 547Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLEX-ray2.00A/B16-549[»]
1LLFX-ray1.40A/B16-549[»]
ProteinModelPortaliP32947.
SMRiP32947.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32947.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLALALSLI ASVAAAPTAK LANGDTITGL NAIINEAFLG IPFAEPPVGN
60 70 80 90 100
LRFKDPVPYS GSLNGQKFTS YGPSCMQQNP EGTFEENLGK TALDLVMQSK
110 120 130 140 150
VFQAVLPQSE DCLTINVVRP PGTKAGANLP VMLWIFGGGF EIGSPTIFPP
160 170 180 190 200
AQMVTKSVLM GKPIIHVAVN YRVASWGFLA GDDIKAEGSG NAGLKDQRLG
210 220 230 240 250
MQWVADNIAG FGGDPSKVTI FGESAGSMSV LCHLIWNDGD NTYKGKPLFR
260 270 280 290 300
AGIMQSGAMV PSDPVDGTYG NEIYDLFVSS AGCGSASDKL ACLRSASSDT
310 320 330 340 350
LLDATNNTPG FLAYSSLRLS YLPRPDGKNI TDDMYKLVRD GKYASVPVII
360 370 380 390 400
GDQNDEGTIF GLSSLNVTTN AQARAYFKQS FIHASDAEID TLMAAYPQDI
410 420 430 440 450
TQGSPFDTGI FNAITPQFKR ISAVLGDLAF IHARRYFLNH FQGGTKYSFL
460 470 480 490 500
SKQLSGLPIM GTFHANDIVW QDYLLGSGSV IYNNAFIAFA TDLDPNTAGL
510 520 530 540
LVNWPKYTSS SQSGNNLMMI NALGLYTGKD NFRTAGYDAL MTNPSSFFV
Length:549
Mass (Da):58,755
Last modified:October 1, 1993 - v1
Checksum:iC5F95C31422975DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66006 Genomic DNA. Translation: CAA46805.1.
PIRiJN0551.
S41735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66006 Genomic DNA. Translation: CAA46805.1.
PIRiJN0551.
S41735.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLEX-ray2.00A/B16-549[»]
1LLFX-ray1.40A/B16-549[»]
ProteinModelPortaliP32947.
SMRiP32947.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERicanru-3lipa. Fungal_carboxylesterase_lipase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.1.3. 1139.

Miscellaneous databases

EvolutionaryTraceiP32947.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIP3_CANRU
AccessioniPrimary (citable) accession number: P32947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.