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P32945

- PPQ1_YEAST

UniProt

P32945 - PPQ1_YEAST

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Protein

Serine/threonine-protein phosphatase PPQ

Gene

PPQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphatase involved in the regulation of protein synthesis. Affects translational accuracy.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi301 – 3011Manganese 1By similarity
Metal bindingi303 – 3031Manganese 1By similarity
Metal bindingi329 – 3291Manganese 1By similarity
Metal bindingi329 – 3291Manganese 2By similarity
Metal bindingi361 – 3611Manganese 2By similarity
Active sitei362 – 3621Proton donorBy similarity
Metal bindingi410 – 4101Manganese 2By similarity
Metal bindingi485 – 4851Manganese 2By similarity

GO - Molecular functioni

  1. cofactor binding Source: InterPro
  2. magnesium-dependent protein serine/threonine phosphatase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. phosphoprotein phosphatase activity Source: SGD
  5. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  2. protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34074-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PPQ (EC:3.1.3.16)
Gene namesi
Name:PPQ1
Synonyms:SAL6
Ordered Locus Names:YPL179W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL179w.
SGDiS000006100. PPQ1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Serine/threonine-protein phosphatase PPQPRO_0000058893Add
BLAST

Proteomic databases

MaxQBiP32945.
PaxDbiP32945.

Expressioni

Gene expression databases

GenevestigatoriP32945.

Interactioni

Protein-protein interaction databases

BioGridi36003. 48 interactions.
DIPiDIP-5504N.
IntActiP32945. 3 interactions.
MINTiMINT-564004.
STRINGi4932.YPL179W.

Structurei

3D structure databases

ProteinModelPortaliP32945.
SMRiP32945. Positions 238-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi113 – 219107Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00710000107291.
HOGENOMiHOG000172697.
InParanoidiP32945.
KOiK06269.
OrthoDBiEOG7M3J8G.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32945-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRSPSRSNN NFAVPNCSTN SNSSQQQLTT PSDDLNSNEP NDPDDSRSLP
60 70 80 90 100
TIKKFNNKHS INNYNTLASA GKNNNNKRAS NDNLLIPGEN AHKQKIYTKD
110 120 130 140 150
ENLKSLYLDI DVSVAKALSS SATAPKLINT ARTSSTTTAT TSNNILTSPS
160 170 180 190 200
YRESNYSSPS SYSFSSYYSS ATSASSSTSS FLKSSGLSSR VKSPSSSVKA
210 220 230 240 250
GSFGAPSSPT SGIPNPKSSK KPIFLRRYSH DTSSNEGLDI DVAIEKLLQV
260 270 280 290 300
GESREITKTS KKKNFPFHSW EIQLICYHAR EIFLNQPTLL RLQAPIKVVG
310 320 330 340 350
DVHGQFNDLL RILKLSGVPS DTNYLFLGDY VDRGKNSLET ILLLLCYKIK
360 370 380 390 400
YKDNFFMLRG NHESANVTKM YGFYDECKRR LSSKVWKMFV DVFNTLPLAA
410 420 430 440 450
IIQDKIFCVH GGISPDLHDM KQIEKVARPT DIPESGLVTD LLWSDPDPQV
460 470 480 490 500
TDWSENDRGV SYTFSKRNVL DFCAKFKFDL ILRGHMVVED GYEFFARKKF
510 520 530 540
VTIFSAPNYC GEFHNWGAVM SVTTGMMCSF ELLKPRALKN KKKLYKTKV
Length:549
Mass (Da):61,420
Last modified:October 1, 1993 - v1
Checksum:i1E26D05E2865A8C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75485 Genomic DNA. Translation: CAA53214.1.
U00795 Genomic DNA. Translation: AAC48924.1.
Z73535 Genomic DNA. Translation: CAA97886.1.
S39958 Genomic DNA. Translation: AAB22461.1.
BK006949 Genomic DNA. Translation: DAA11255.1.
PIRiS39533.
RefSeqiNP_015146.1. NM_001183993.1.

Genome annotation databases

EnsemblFungiiYPL179W; YPL179W; YPL179W.
GeneIDi855923.
KEGGisce:YPL179W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75485 Genomic DNA. Translation: CAA53214.1 .
U00795 Genomic DNA. Translation: AAC48924.1 .
Z73535 Genomic DNA. Translation: CAA97886.1 .
S39958 Genomic DNA. Translation: AAB22461.1 .
BK006949 Genomic DNA. Translation: DAA11255.1 .
PIRi S39533.
RefSeqi NP_015146.1. NM_001183993.1.

3D structure databases

ProteinModelPortali P32945.
SMRi P32945. Positions 238-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36003. 48 interactions.
DIPi DIP-5504N.
IntActi P32945. 3 interactions.
MINTi MINT-564004.
STRINGi 4932.YPL179W.

Proteomic databases

MaxQBi P32945.
PaxDbi P32945.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL179W ; YPL179W ; YPL179W .
GeneIDi 855923.
KEGGi sce:YPL179W.

Organism-specific databases

CYGDi YPL179w.
SGDi S000006100. PPQ1.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00710000107291.
HOGENOMi HOG000172697.
InParanoidi P32945.
KOi K06269.
OrthoDBi EOG7M3J8G.

Enzyme and pathway databases

BioCyci YEAST:G3O-34074-MONOMER.

Miscellaneous databases

NextBioi 980649.

Gene expression databases

Genevestigatori P32945.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PPQ, a novel protein phosphatase containing a Ser + Asn-rich amino-terminal domain, is involved in the regulation of protein synthesis."
    Chen M.X., Chen Y.H., Cohen P.T.W.
    Eur. J. Biochem. 218:689-699(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Erratum
    Chen M.X., Chen Y.H., Cohen P.T.W.
    Eur. J. Biochem. 221:1133-1133(1994)
  3. "The yeast translational allosuppressor, SAL6: a new member of the PP1-like phosphatase family with a long serine-rich N-terminal extension."
    Vincent A., Newnam G.P., Liebman S.W.
    Genetics 138:597-607(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatases."
    Chen M.X., Chen Y.H., Cohen P.T.W.
    FEBS Lett. 306:54-58(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-328.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPQ1_YEAST
AccessioniPrimary (citable) accession number: P32945
Secondary accession number(s): D6W3I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3