Reviewed, UniProtKB/Swiss-Prot P32945 (PPQ1_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase PPQ
    EC=3.1.3.16

Gene names

Name:	PPQ1
Synonyms:	SAL6
Ordered Locus Names:	YPL179W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphatase involved in the regulation of protein synthesis. Affects translational accuracy.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Miscellaneous	Present with 319 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the PPP phosphatase family. PP-Z subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of translation Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-dependent protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SDS22	P36047	1	EBI-13787,EBI-16783

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Serine/threonine-protein phosphatase PPQ		PRO_0000058893
Regions
	Compositional bias	113 – 219	107	Ser-rich
Sites
	Active site	362	1	Proton donor By similarity
	Metal binding	301	1	Iron By similarity
	Metal binding	303	1	Iron By similarity
	Metal binding	329	1	Iron By similarity
	Metal binding	329	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	410	1	Manganese By similarity
	Metal binding	485	1	Manganese By similarity
Amino acid modifications
	Modified residue	80	1	Phosphoserine Ref.8
	Modified residue	208	1	Phosphoserine Ref.8 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P32945-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1E26D05E2865A8C1	FASTA	549	61,420
	10 20 30 40 50 60 MRRSPSRSNN NFAVPNCSTN SNSSQQQLTT PSDDLNSNEP NDPDDSRSLP TIKKFNNKHS 70 80 90 100 110 120 INNYNTLASA GKNNNNKRAS NDNLLIPGEN AHKQKIYTKD ENLKSLYLDI DVSVAKALSS 130 140 150 160 170 180 SATAPKLINT ARTSSTTTAT TSNNILTSPS YRESNYSSPS SYSFSSYYSS ATSASSSTSS 190 200 210 220 230 240 FLKSSGLSSR VKSPSSSVKA GSFGAPSSPT SGIPNPKSSK KPIFLRRYSH DTSSNEGLDI 250 260 270 280 290 300 DVAIEKLLQV GESREITKTS KKKNFPFHSW EIQLICYHAR EIFLNQPTLL RLQAPIKVVG 310 320 330 340 350 360 DVHGQFNDLL RILKLSGVPS DTNYLFLGDY VDRGKNSLET ILLLLCYKIK YKDNFFMLRG 370 380 390 400 410 420 NHESANVTKM YGFYDECKRR LSSKVWKMFV DVFNTLPLAA IIQDKIFCVH GGISPDLHDM 430 440 450 460 470 480 KQIEKVARPT DIPESGLVTD LLWSDPDPQV TDWSENDRGV SYTFSKRNVL DFCAKFKFDL 490 500 510 520 530 540 ILRGHMVVED GYEFFARKKF VTIFSAPNYC GEFHNWGAVM SVTTGMMCSF ELLKPRALKN KKKLYKTKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PPQ, a novel protein phosphatase containing a Ser + Asn-rich amino-terminal domain, is involved in the regulation of protein synthesis." Chen M.X., Chen Y.H., Cohen P.T.W. Eur. J. Biochem. 218:689-699(1993) [PubMed: 8269960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Erratum Chen M.X., Chen Y.H., Cohen P.T.W. Eur. J. Biochem. 221:1133-1133(1994)
[3]	"The yeast translational allosuppressor, SAL6: a new member of the PP1-like phosphatase family with a long serine-rich N-terminal extension." Vincent A., Newnam G.P., Liebman S.W. Genetics 138:597-607(1994) [PubMed: 7851758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatases." Chen M.X., Chen Y.H., Cohen P.T.W. FEBS Lett. 306:54-58(1992) [PubMed: 1321058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-328.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-208, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75485 Genomic DNA. Translation: CAA53214.1. U00795 Genomic DNA. Translation: AAC48924.1. Z73535 Genomic DNA. Translation: CAA97886.1. S39958 Genomic DNA. Translation: AAB22461.1.
PIR	S39533.
RefSeq	NP_015146.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5504N.
IntAct	P32945. 4 interactions.
STRING	P32945.
Genome annotation databases
Ensembl	YPL179W; YPL179W; YPL179W; Saccharomyces cerevisiae. [Genome view]
GeneID	855923.
KEGG	sce:YPL179W.
NMPDR	fig|4932.3.peg.6275.
Organism-specific databases
CYGD	YPL179w.
SGD	S000006100. PPQ1.
Phylogenomic databases
HOGENOM	P32945.
OrthoDB	EOG9XD582
Enzyme and pathway databases
BRENDA	3.1.3.16. 250.
Gene expression databases
ArrayExpress	P32945.
Genevestigator	P32945.
GermOnline	YPL179W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR011159. PPPtase_PPZ. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
PANTHER	PTHR11668. T_phtase_apaH. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000909. PPPtase_PPZ. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980649.

Entry information

Entry name

PPQ1_YEAST

Accession

Primary (citable) accession number: P32945

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)