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P32945

- PPQ1_YEAST

UniProt

P32945 - PPQ1_YEAST

Protein

Serine/threonine-protein phosphatase PPQ

Gene

PPQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Phosphatase involved in the regulation of protein synthesis. Affects translational accuracy.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi301 – 3011Manganese 1By similarity
    Metal bindingi303 – 3031Manganese 1By similarity
    Metal bindingi329 – 3291Manganese 1By similarity
    Metal bindingi329 – 3291Manganese 2By similarity
    Metal bindingi361 – 3611Manganese 2By similarity
    Active sitei362 – 3621Proton donorBy similarity
    Metal bindingi410 – 4101Manganese 2By similarity
    Metal bindingi485 – 4851Manganese 2By similarity

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. magnesium-dependent protein serine/threonine phosphatase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. phosphoprotein phosphatase activity Source: SGD
    5. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    2. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34074-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PPQ (EC:3.1.3.16)
    Gene namesi
    Name:PPQ1
    Synonyms:SAL6
    Ordered Locus Names:YPL179W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL179w.
    SGDiS000006100. PPQ1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Serine/threonine-protein phosphatase PPQPRO_0000058893Add
    BLAST

    Proteomic databases

    MaxQBiP32945.
    PaxDbiP32945.

    Expressioni

    Gene expression databases

    GenevestigatoriP32945.

    Interactioni

    Protein-protein interaction databases

    BioGridi36003. 47 interactions.
    DIPiDIP-5504N.
    IntActiP32945. 3 interactions.
    MINTiMINT-564004.
    STRINGi4932.YPL179W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32945.
    SMRiP32945. Positions 238-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi113 – 219107Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00710000107291.
    HOGENOMiHOG000172697.
    KOiK06269.
    OrthoDBiEOG7M3J8G.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32945-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRSPSRSNN NFAVPNCSTN SNSSQQQLTT PSDDLNSNEP NDPDDSRSLP    50
    TIKKFNNKHS INNYNTLASA GKNNNNKRAS NDNLLIPGEN AHKQKIYTKD 100
    ENLKSLYLDI DVSVAKALSS SATAPKLINT ARTSSTTTAT TSNNILTSPS 150
    YRESNYSSPS SYSFSSYYSS ATSASSSTSS FLKSSGLSSR VKSPSSSVKA 200
    GSFGAPSSPT SGIPNPKSSK KPIFLRRYSH DTSSNEGLDI DVAIEKLLQV 250
    GESREITKTS KKKNFPFHSW EIQLICYHAR EIFLNQPTLL RLQAPIKVVG 300
    DVHGQFNDLL RILKLSGVPS DTNYLFLGDY VDRGKNSLET ILLLLCYKIK 350
    YKDNFFMLRG NHESANVTKM YGFYDECKRR LSSKVWKMFV DVFNTLPLAA 400
    IIQDKIFCVH GGISPDLHDM KQIEKVARPT DIPESGLVTD LLWSDPDPQV 450
    TDWSENDRGV SYTFSKRNVL DFCAKFKFDL ILRGHMVVED GYEFFARKKF 500
    VTIFSAPNYC GEFHNWGAVM SVTTGMMCSF ELLKPRALKN KKKLYKTKV 549
    Length:549
    Mass (Da):61,420
    Last modified:October 1, 1993 - v1
    Checksum:i1E26D05E2865A8C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75485 Genomic DNA. Translation: CAA53214.1.
    U00795 Genomic DNA. Translation: AAC48924.1.
    Z73535 Genomic DNA. Translation: CAA97886.1.
    S39958 Genomic DNA. Translation: AAB22461.1.
    BK006949 Genomic DNA. Translation: DAA11255.1.
    PIRiS39533.
    RefSeqiNP_015146.1. NM_001183993.1.

    Genome annotation databases

    EnsemblFungiiYPL179W; YPL179W; YPL179W.
    GeneIDi855923.
    KEGGisce:YPL179W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75485 Genomic DNA. Translation: CAA53214.1 .
    U00795 Genomic DNA. Translation: AAC48924.1 .
    Z73535 Genomic DNA. Translation: CAA97886.1 .
    S39958 Genomic DNA. Translation: AAB22461.1 .
    BK006949 Genomic DNA. Translation: DAA11255.1 .
    PIRi S39533.
    RefSeqi NP_015146.1. NM_001183993.1.

    3D structure databases

    ProteinModelPortali P32945.
    SMRi P32945. Positions 238-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36003. 47 interactions.
    DIPi DIP-5504N.
    IntActi P32945. 3 interactions.
    MINTi MINT-564004.
    STRINGi 4932.YPL179W.

    Proteomic databases

    MaxQBi P32945.
    PaxDbi P32945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL179W ; YPL179W ; YPL179W .
    GeneIDi 855923.
    KEGGi sce:YPL179W.

    Organism-specific databases

    CYGDi YPL179w.
    SGDi S000006100. PPQ1.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00710000107291.
    HOGENOMi HOG000172697.
    KOi K06269.
    OrthoDBi EOG7M3J8G.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34074-MONOMER.

    Miscellaneous databases

    NextBioi 980649.

    Gene expression databases

    Genevestigatori P32945.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PPQ, a novel protein phosphatase containing a Ser + Asn-rich amino-terminal domain, is involved in the regulation of protein synthesis."
      Chen M.X., Chen Y.H., Cohen P.T.W.
      Eur. J. Biochem. 218:689-699(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Erratum
      Chen M.X., Chen Y.H., Cohen P.T.W.
      Eur. J. Biochem. 221:1133-1133(1994)
    3. "The yeast translational allosuppressor, SAL6: a new member of the PP1-like phosphatase family with a long serine-rich N-terminal extension."
      Vincent A., Newnam G.P., Liebman S.W.
      Genetics 138:597-607(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatases."
      Chen M.X., Chen Y.H., Cohen P.T.W.
      FEBS Lett. 306:54-58(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-328.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPQ1_YEAST
    AccessioniPrimary (citable) accession number: P32945
    Secondary accession number(s): D6W3I9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 319 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3