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P32942

- ICAM3_HUMAN

UniProt

P32942 - ICAM3_HUMAN

Protein

Intercellular adhesion molecule 3

Gene

ICAM3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 2 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

    GO - Molecular functioni

    1. integrin binding Source: BHF-UCL
    2. protein binding Source: BHF-UCL
    3. receptor binding Source: ProtInc

    GO - Biological processi

    1. extracellular matrix organization Source: Reactome
    2. regulation of immune response Source: Reactome
    3. single organismal cell-cell adhesion Source: InterPro

    Keywords - Biological processi

    Cell adhesion

    Enzyme and pathway databases

    ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_13552. Integrin cell surface interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intercellular adhesion molecule 3
    Short name:
    ICAM-3
    Alternative name(s):
    CDw50
    ICAM-R
    CD_antigen: CD50
    Gene namesi
    Name:ICAM3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:5346. ICAM3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29594.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 547518Intercellular adhesion molecule 3PRO_0000014794Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi52 – 521N-linked (GlcNAc...)1 Publication
    Disulfide bondi53 ↔ 961 PublicationPROSITE-ProRule annotation
    Glycosylationi84 – 841N-linked (GlcNAc...)1 Publication
    Glycosylationi87 – 871N-linked (GlcNAc...)1 Publication
    Glycosylationi91 – 911N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...)1 Publication
    Glycosylationi134 – 1341N-linked (GlcNAc...)1 Publication
    Disulfide bondi139 ↔ 190PROSITE-ProRule annotation
    Glycosylationi206 – 2061N-linked (GlcNAc...)3 Publications
    Disulfide bondi241 ↔ 294PROSITE-ProRule annotation
    Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi295 – 2951N-linked (GlcNAc...)1 Publication
    Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi336 ↔ 375PROSITE-ProRule annotation
    Glycosylationi363 – 3631N-linked (GlcNAc...)3 Publications
    Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi423 ↔ 462PROSITE-ProRule annotation
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.4 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP32942.
    PaxDbiP32942.
    PRIDEiP32942.

    PTM databases

    PhosphoSiteiP32942.
    UniCarbKBiP32942.

    Expressioni

    Gene expression databases

    ArrayExpressiP32942.
    BgeeiP32942.
    CleanExiHS_ICAM3.
    GenevestigatoriP32942.

    Organism-specific databases

    HPAiCAB002498.

    Interactioni

    Protein-protein interaction databases

    BioGridi109612. 5 interactions.
    IntActiP32942. 3 interactions.
    MINTiMINT-1391707.
    STRINGi9606.ENSP00000160262.

    Structurei

    Secondary structure

    1
    547
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 385
    Beta strandi48 – 558
    Beta strandi61 – 666
    Beta strandi68 – 7710
    Beta strandi80 – 867
    Beta strandi92 – 1009
    Beta strandi103 – 11311

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T0PX-ray1.66B30-114[»]
    ProteinModelPortaliP32942.
    SMRiP32942. Positions 30-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32942.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 485456ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini511 – 54737CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei486 – 51025HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 10358Ig-like C2-type 1Add
    BLAST
    Domaini132 – 19766Ig-like C2-type 2Add
    BLAST
    Domaini234 – 30168Ig-like C2-type 3Add
    BLAST
    Domaini329 – 38254Ig-like C2-type 4Add
    BLAST
    Domaini416 – 46954Ig-like C2-type 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the immunoglobulin superfamily. ICAM family.Curated

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG146347.
    HOGENOMiHOG000059554.
    HOVERGENiHBG052074.
    InParanoidiP32942.
    KOiK06486.
    OMAiYGPKIDR.
    OrthoDBiEOG7QG43X.
    PhylomeDBiP32942.
    TreeFamiTF333745.

    Family and domain databases

    Gene3Di2.60.40.10. 5 hits.
    InterProiIPR003988. ICAM.
    IPR013768. ICAM_N.
    IPR003987. ICAM_VCAM_N.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    [Graphical view]
    PfamiPF03921. ICAM_N. 1 hit.
    [Graphical view]
    PRINTSiPR01473. ICAM.
    PR01472. ICAMVCAM1.
    SMARTiSM00409. IG. 2 hits.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32942-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATMVPSVLW PRACWTLLVC CLLTPGVQGQ EFLLRVEPQN PVLSAGGSLF    50
    VNCSTDCPSS EKIALETSLS KELVASGMGW AAFNLSNVTG NSRILCSVYC 100
    NGSQITGSSN ITVYRLPERV ELAPLPPWQP VGQNFTLRCQ VEDGSPRTSL 150
    TVVLLRWEEE LSRQPAVEEP AEVTATVLAS RDDHGAPFSC RTELDMQPQG 200
    LGLFVNTSAP RQLRTFVLPV TPPRLVAPRF LEVETSWPVD CTLDGLFPAS 250
    EAQVYLALGD QMLNATVMNH GDTLTATATA TARADQEGAR EIVCNVTLGG 300
    ERREARENLT VFSFLGPIVN LSEPTAHEGS TVTVSCMAGA RVQVTLDGVP 350
    AAAPGQPAQL QLNATESDDG RSFFCSATLE VDGEFLHRNS SVQLRVLYGP 400
    KIDRATCPQH LKWKDKTRHV LQCQARGNPY PELRCLKEGS SREVPVGIPF 450
    FVNVTHNGTY QCQASSSRGK YTLVVVMDIE AGSSHFVPVF VAVLLTLGVV 500
    TIVLALMYVF REHQRSGSYH VREESTYLPL TSMQPTEAMG EEPSRAE 547
    Length:547
    Mass (Da):59,541
    Last modified:September 23, 2008 - v2
    Checksum:i4B7BDC02F24F3031
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601S → F in AAB24331. (PubMed:1448173)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti63 – 631I → V.
    Corresponds to variant rs17697947 [ dbSNP | Ensembl ].
    VAR_046547
    Natural varianti115 – 1151R → G.2 Publications
    Corresponds to variant rs7258015 [ dbSNP | Ensembl ].
    VAR_059394
    Natural varianti143 – 1431D → G.3 Publications
    Corresponds to variant rs2304237 [ dbSNP | Ensembl ].
    VAR_046548
    Natural varianti525 – 5251S → T.
    Corresponds to variant rs2230399 [ dbSNP | Ensembl ].
    VAR_024498

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S50015 mRNA. Translation: AAB24331.2. Sequence problems.
    X69711 mRNA. Translation: CAA49369.1.
    X69819 mRNA. Translation: CAA49473.1.
    DQ217937 Genomic DNA. Translation: ABB01007.1.
    CH471106 Genomic DNA. Translation: EAW84092.1.
    BC058903 mRNA. Translation: AAH58903.1.
    CCDSiCCDS12235.1.
    PIRiS28904.
    RefSeqiNP_002153.2. NM_002162.3.
    UniGeneiHs.654563.

    Genome annotation databases

    EnsembliENST00000160262; ENSP00000160262; ENSG00000076662.
    GeneIDi3385.
    KEGGihsa:3385.
    UCSCiuc002mob.2. human.

    Polymorphism databases

    DMDMi206729872.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    R&D Systems' cytokine source book: ICAM-3
    Functional Glycomics Gateway - Glycan Binding

    ICAM-3

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S50015 mRNA. Translation: AAB24331.2 . Sequence problems.
    X69711 mRNA. Translation: CAA49369.1 .
    X69819 mRNA. Translation: CAA49473.1 .
    DQ217937 Genomic DNA. Translation: ABB01007.1 .
    CH471106 Genomic DNA. Translation: EAW84092.1 .
    BC058903 mRNA. Translation: AAH58903.1 .
    CCDSi CCDS12235.1.
    PIRi S28904.
    RefSeqi NP_002153.2. NM_002162.3.
    UniGenei Hs.654563.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1T0P X-ray 1.66 B 30-114 [» ]
    ProteinModelPortali P32942.
    SMRi P32942. Positions 30-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109612. 5 interactions.
    IntActi P32942. 3 interactions.
    MINTi MINT-1391707.
    STRINGi 9606.ENSP00000160262.

    PTM databases

    PhosphoSitei P32942.
    UniCarbKBi P32942.

    Polymorphism databases

    DMDMi 206729872.

    Proteomic databases

    MaxQBi P32942.
    PaxDbi P32942.
    PRIDEi P32942.

    Protocols and materials databases

    DNASUi 3385.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000160262 ; ENSP00000160262 ; ENSG00000076662 .
    GeneIDi 3385.
    KEGGi hsa:3385.
    UCSCi uc002mob.2. human.

    Organism-specific databases

    CTDi 3385.
    GeneCardsi GC19M010444.
    H-InvDB HIX0014743.
    HGNCi HGNC:5346. ICAM3.
    HPAi CAB002498.
    MIMi 146631. gene.
    neXtProti NX_P32942.
    PharmGKBi PA29594.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG146347.
    HOGENOMi HOG000059554.
    HOVERGENi HBG052074.
    InParanoidi P32942.
    KOi K06486.
    OMAi YGPKIDR.
    OrthoDBi EOG7QG43X.
    PhylomeDBi P32942.
    TreeFami TF333745.

    Enzyme and pathway databases

    Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_13552. Integrin cell surface interactions.

    Miscellaneous databases

    EvolutionaryTracei P32942.
    GeneWikii ICAM3.
    GenomeRNAii 3385.
    NextBioi 13392.
    PROi P32942.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P32942.
    Bgeei P32942.
    CleanExi HS_ICAM3.
    Genevestigatori P32942.

    Family and domain databases

    Gene3Di 2.60.40.10. 5 hits.
    InterProi IPR003988. ICAM.
    IPR013768. ICAM_N.
    IPR003987. ICAM_VCAM_N.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    [Graphical view ]
    Pfami PF03921. ICAM_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01473. ICAM.
    PR01472. ICAMVCAM1.
    SMARTi SM00409. IG. 2 hits.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes."
      Fawcett J., Holness C.L., Needham L.A., Turley H., Gatter K.C., Mason D.Y., Simmons D.L.
      Nature 360:481-484(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS AND GLY-115 GLY-143.
    2. "Cloning and characterization of a new intercellular adhesion molecule ICAM-R."
      Vazeux R., Hoffman P.A., Tomita J.K., Dickinson E.S., Jasman R.L., St John T., Gallatin W.M.
      Nature 360:485-488(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-115 AND GLY-143.
    3. "Cloning and expression of intercellular adhesion molecule 3 reveals strong homology to other immunoglobulin family counter-receptors for lymphocyte function-associated antigen 1."
      de Fougerolles A.R., Kilckstein L.B., Springer T.A.
      J. Exp. Med. 177:1187-1192(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-115 AND GLY-143.
    4. "Genetic variation in immune response genes."
      Tan J., Ong R., Hibberd M.L., Seielstad M.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    7. Cited for: PROTEIN SEQUENCE OF 197-217; 269-293 AND 338-365.
    8. "Structural study of N-linked oligosaccharides of human intercellular adhesion molecule-3 (CD50)."
      Funatsu O., Sato T., Kotovuori P., Gahmberg C.G., Ikekita M., Furukawa K.
      Eur. J. Biochem. 268:1020-1029(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF N-LINKED CARBOHYDRATES.
    9. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206 AND ASN-363.
      Tissue: Plasma.
    10. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206 AND ASN-363.
      Tissue: Liver.
    11. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-84; ASN-87; ASN-91; ASN-134; ASN-206; ASN-295 AND ASN-363.
      Tissue: Leukemic T-cell.
    12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2."
      Song G., Yang Y., Liu J.-H., Casasnovas J.M., Shimaoka M., Springer T.A., Wang J.-H.
      Proc. Natl. Acad. Sci. U.S.A. 102:3366-3371(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 30-115 IN COMPLEX WITH INTEGRIN ALPHALBETA2, DISULFIDE BOND, GLYCOSYLATION AT ASN-52 AND ASN-110.

    Entry informationi

    Entry nameiICAM3_HUMAN
    AccessioniPrimary (citable) accession number: P32942
    Secondary accession number(s): Q6PD68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 155 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3