Reviewed, UniProtKB/Swiss-Prot P32942 (ICAM3_HUMAN)

Names and origin

Protein names

Recommended name:
    Intercellular adhesion molecule 3
      Short name=ICAM-3
Alternative name(s):
    ICAM-R
    CDw50
    CD_antigen=CD50

Gene names

Name:

ICAM3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. Ref.8 Ref.9 Ref.11 Ref.12 Ref.13
Sequence similarities	Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Ref.3 Traceable author statement. Source: ProtInc
Molecular function	integrin binding Ref.1 Ref.2 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ITGAL	P20701	1	EBI-725421,EBI-961214

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Potential

Chain

30 – 547

518

Intercellular adhesion molecule 3

PRO_0000014794

Regions

Topological domain

30 – 485

456

Extracellular Potential

Transmembrane

486 – 510

25

Potential

Topological domain

511 – 547

37

Cytoplasmic Potential

Domain

46 – 103

58

Ig-like C2-type 1

Domain

132 – 197

66

Ig-like C2-type 2

Domain

234 – 301

68

Ig-like C2-type 3

Domain

329 – 382

54

Ig-like C2-type 4

Domain

416 – 469

54

Ig-like C2-type 5

Amino acid modifications

Glycosylation

52

1

N-linked (GlcNAc...) Ref.13

Glycosylation

84

1

N-linked (GlcNAc...) Ref.12

Glycosylation

87

1

N-linked (GlcNAc...) Ref.12

Glycosylation

91

1

N-linked (GlcNAc...) Ref.12

Glycosylation

101

1

N-linked (GlcNAc...) Potential

Glycosylation

110

1

N-linked (GlcNAc...) Ref.13

Glycosylation

134

1

N-linked (GlcNAc...) Ref.12

Glycosylation

206

1

N-linked (GlcNAc...) Ref.9 Ref.11 Ref.12

Glycosylation

264

1

N-linked (GlcNAc...) Potential

Glycosylation

295

1

N-linked (GlcNAc...) Ref.12

Glycosylation

308

1

N-linked (GlcNAc...) Potential

Glycosylation

320

1

N-linked (GlcNAc...) Potential

Glycosylation

363

1

N-linked (GlcNAc...) Ref.9 Ref.11 Ref.12

Glycosylation

389

1

N-linked (GlcNAc...) Potential

Glycosylation

453

1

N-linked (GlcNAc...) Potential

Glycosylation

457

1

N-linked (GlcNAc...) Potential

Disulfide bond

53 ↔ 96

Ref.13

Disulfide bond

139 ↔ 190

Potential

Disulfide bond

241 ↔ 294

Potential

Disulfide bond

336 ↔ 375

Potential

Disulfide bond

423 ↔ 462

Potential

Natural variations

Natural variant

63

1

I → V: dbSNP rs17697947.

VAR_046547

Natural variant

115

1

R → G: dbSNP rs7258015. Ref.1 Ref.2 Ref.3

VAR_059394

Natural variant

143

1

D → G: dbSNP rs2304237. Ref.1 Ref.2 Ref.3

VAR_046548

Natural variant

525

1

S → T: dbSNP rs2230399.

VAR_024498

Experimental info

Sequence conflict

60

1

S → F in AAB24331. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

547

Helix Strand Turn

Details...

Beta strand

34 – 38

5

Beta strand

48 – 55

8

Beta strand

61 – 66

6

Beta strand

68 – 77

10

Beta strand

80 – 86

7

Beta strand

92 – 100

9

Beta strand

103 – 113

11

Sequences

	Sequence		Length	Mass (Da)
	P32942-1 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 4B7BDC02F24F3031	FASTA	547	59,541
	10 20 30 40 50 60 MATMVPSVLW PRACWTLLVC CLLTPGVQGQ EFLLRVEPQN PVLSAGGSLF VNCSTDCPSS 70 80 90 100 110 120 EKIALETSLS KELVASGMGW AAFNLSNVTG NSRILCSVYC NGSQITGSSN ITVYRLPERV 130 140 150 160 170 180 ELAPLPPWQP VGQNFTLRCQ VEDGSPRTSL TVVLLRWEEE LSRQPAVEEP AEVTATVLAS 190 200 210 220 230 240 RDDHGAPFSC RTELDMQPQG LGLFVNTSAP RQLRTFVLPV TPPRLVAPRF LEVETSWPVD 250 260 270 280 290 300 CTLDGLFPAS EAQVYLALGD QMLNATVMNH GDTLTATATA TARADQEGAR EIVCNVTLGG 310 320 330 340 350 360 ERREARENLT VFSFLGPIVN LSEPTAHEGS TVTVSCMAGA RVQVTLDGVP AAAPGQPAQL 370 380 390 400 410 420 QLNATESDDG RSFFCSATLE VDGEFLHRNS SVQLRVLYGP KIDRATCPQH LKWKDKTRHV 430 440 450 460 470 480 LQCQARGNPY PELRCLKEGS SREVPVGIPF FVNVTHNGTY QCQASSSRGK YTLVVVMDIE 490 500 510 520 530 540 AGSSHFVPVF VAVLLTLGVV TIVLALMYVF REHQRSGSYH VREESTYLPL TSMQPTEAMG EEPSRAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes." Fawcett J., Holness C.L., Needham L.A., Turley H., Gatter K.C., Mason D.Y., Simmons D.L. Nature 360:481-484(1992) [PubMed: 1448173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS AND GLY-115 GLY-143.
[2]	"Cloning and characterization of a new intercellular adhesion molecule ICAM-R." Vazeux R., Hoffman P.A., Tomita J.K., Dickinson E.S., Jasman R.L., St John T., Gallatin W.M. Nature 360:485-488(1992) [PubMed: 1448174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-115 AND GLY-143.
[3]	"Cloning and expression of intercellular adhesion molecule 3 reveals strong homology to other immunoglobulin family counter-receptors for lymphocyte function-associated antigen 1." de Fougerolles A.R., Kilckstein L.B., Springer T.A. J. Exp. Med. 177:1187-1192(1993) [PubMed: 8459213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-115 AND GLY-143.
[4]	"Genetic variation in immune response genes." Tan J., Ong R., Hibberd M.L., Seielstad M. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"CDw50 and ICAM-3: two names for the same molecule." Juan M., Vilella R., Mila J., Yague J., Miralles A., Campbell K.S., Friedrich R.J., Cambier J., Vives J., de Fougerolles A.R., Springer T.A. Eur. J. Immunol. 23:1508-1512(1993) [PubMed: 8325327] [Abstract] Cited for: PROTEIN SEQUENCE OF 197-217; 269-293 AND 338-365.
[8]	"Structural study of N-linked oligosaccharides of human intercellular adhesion molecule-3 (CD50)." Funatsu O., Sato T., Kotovuori P., Gahmberg C.G., Ikekita M., Furukawa K. Eur. J. Biochem. 268:1020-1029(2001) [PubMed: 11179968] [Abstract] Cited for: STRUCTURE OF N-LINKED CARBOHYDRATES.
[9]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206 AND ASN-363, MASS SPECTROMETRY. Tissue: Plasma.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206 AND ASN-363, MASS SPECTROMETRY. Tissue: Liver.
[12]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-84; ASN-87; ASN-91; ASN-134; ASN-206; ASN-295 AND ASN-363, MASS SPECTROMETRY.
[13]	"An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2." Song G., Yang Y., Liu J.-H., Casasnovas J.M., Shimaoka M., Springer T.A., Wang J.-H. Proc. Natl. Acad. Sci. U.S.A. 102:3366-3371(2005) [PubMed: 15728350] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 30-115 IN COMPLEX WITH INTEGRIN ALPHALBETA2, DISULFIDE BOND, GLYCOSYLATION AT ASN-52 AND ASN-110.
+	Additional computationally mapped references.

Web resources

R&D Systems' cytokine source book: ICAM-3

Functional Glycomics Gateway - Glycan Binding

ICAM-3

Cross-references

Sequence databases

EMBLGenBankDDBJ

S50015 mRNA. Translation: AAB24331.2. Sequence problems.
X69711 mRNA. Translation: CAA49369.1.
X69819 mRNA. Translation: CAA49473.1.
DQ217937 Genomic DNA. Translation: ABB01007.1.
CH471106 Genomic DNA. Translation: EAW84092.1.
BC058903 mRNA. Translation: AAH58903.1.

IPI

IPI00031620.

PIR

S28904.

RefSeq

NP_002153.2.

UniGene

Hs.654563

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T0P	X-ray	1.66	B	30-114	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P32942. 2 interactions.

STRING

P32942.

PTM databases

GlycoSuiteDB

P32942.

PhosphoSite

P32942.

Proteomic databases

PRIDE

P32942.

Genome annotation databases

Ensembl

ENST00000160262; ENSP00000160262; ENSG00000076662; Homo sapiens. [Genome view]

GeneID

3385.

KEGG

hsa:3385.

UCSC

uc002mob.2. human.

Organism-specific databases

CTD

3385.

GeneCards

GC19M010305.

H-InvDB

HIX0039997.

HGNC

HGNC:5346. ICAM3.

HPA

CAB002498.

MIM

146631. gene.

PharmGKB

PA29594.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P32942.

HOVERGEN

P32942.

OMA

YGPKIDR.

Enzyme and pathway databases

Reactome

REACT_13552. Integrin cell surface interactions.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpress

P32942.

Bgee

P32942.

CleanEx

HS_ICAM3.

Genevestigator

P32942.

GermOnline

ENSG00000076662. Homo sapiens.

Family and domain databases

InterPro

IPR003988. ICAM.
IPR013768. ICAM_N.
IPR003987. ICAM_VCAM_N.
IPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 3 hits.

Pfam

PF03921. ICAM_N. 1 hit.
PF00047. ig. 1 hit.
[Graphical view]

PRINTS

PR01473. ICAM.
PR01472. ICAMVCAM1.

SMART

SM00409. IG. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

ICAM3_HUMAN

Accession

Primary (citable) accession number: P32942
Secondary accession number(s): Q6PD68

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.