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Protein

GTP-binding protein YPT7

Gene

YPT7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

Miscellaneous

Present with 5530 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi64 – 68GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • endocytosis Source: SGD
  • macroautophagy Source: SGD
  • piecemeal microautophagy of the nucleus Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein localization to vacuole Source: SGD
  • protein transport Source: UniProtKB-KW
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • retrograde transport, endosome to Golgi Source: SGD
  • vacuole inheritance Source: SGD
  • vesicle-mediated transport Source: SGD

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-8854214 TBC/RABGAPs
R-SCE-8873719 RAB geranylgeranylation

Protein family/group databases

TCDBi9.A.63.1.1 the retromer-dependent vacuolar protein sorting (r-vps) family

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT7
Gene namesi
Name:YPT7
Synonyms:VAM4
Ordered Locus Names:YML001W
ORF Names:YM8270.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML001W
SGDiS000004460 YPT7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001213201 – 208GTP-binding protein YPT7Add BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Lipidationi206S-geranylgeranyl cysteineBy similarity1
Modified residuei208Cysteine methyl esterBy similarity1
Lipidationi208S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Methylation, Prenylation, Ubl conjugation

Proteomic databases

MaxQBiP32939
PaxDbiP32939
PRIDEiP32939
TopDownProteomicsiP32939

PTM databases

iPTMnetiP32939

Interactioni

Subunit structurei

Interacts with IVY1, YIF1, YIP4 and YIP5.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35170, 453 interactors
DIPiDIP-1735N
IntActiP32939, 23 interactors
MINTiP32939
STRINGi4932.YML001W

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi21 – 30Combined sources10
Beta strandi46 – 50Combined sources5
Beta strandi53 – 55Combined sources3
Beta strandi58 – 63Combined sources6
Helixi69 – 71Combined sources3
Turni73 – 76Combined sources4
Helixi77 – 79Combined sources3
Beta strandi84 – 90Combined sources7
Helixi94 – 98Combined sources5
Helixi100 – 111Combined sources12
Turni116 – 118Combined sources3
Beta strandi121 – 126Combined sources6
Helixi132 – 134Combined sources3
Helixi139 – 148Combined sources10
Beta strandi154 – 158Combined sources5
Turni159 – 162Combined sources4
Helixi165 – 180Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939
SMRiP32939
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32939

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119125
HOGENOMiHOG000233968
InParanoidiP32939
KOiK07897
OMAiLMQQFVN
OrthoDBiEOG092C5MDH

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

P32939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV
60 70 80 90 100
TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI
110 120 130 140 150
KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG
160 170 180 190 200
DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD

GENNSCSC
Length:208
Mass (Da):23,043
Last modified:October 1, 1993 - v1
Checksum:i0E2B72BDA7F7CEE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68144 Genomic DNA Translation: CAA48244.1
D64114 Genomic DNA Translation: BAA10973.1
Z48613 Genomic DNA Translation: CAA88515.1
BK006946 Genomic DNA Translation: DAA09898.1
PIRiA44334
RefSeqiNP_013713.1, NM_001182356.1

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W
GeneIDi855012
KEGGisce:YML001W

Similar proteinsi

Entry informationi

Entry nameiYPT7_YEAST
AccessioniPrimary (citable) accession number: P32939
Secondary accession number(s): D6VZH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health