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Protein

GTP-binding protein YPT7

Gene

YPT7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • endocytosis Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • protein localization to vacuole Source: SGD
  • protein transport Source: UniProtKB-KW
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • retrograde transport, endosome to Golgi Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
  • vacuole inheritance Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT7
Gene namesi
Name:YPT7
Synonyms:VAM4
Ordered Locus Names:YML001W
ORF Names:YM8270.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML001w.
EuPathDBiFungiDB:YML001W.
SGDiS000004460. YPT7.

Subcellular locationi

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208GTP-binding protein YPT7PRO_0000121320Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi206 – 2061S-geranylgeranyl cysteineBy similarity
Modified residuei208 – 2081Cysteine methyl esterBy similarity
Lipidationi208 – 2081S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP32939.
PaxDbiP32939.
PeptideAtlasiP32939.
PRIDEiP32939.

Interactioni

Subunit structurei

Interacts with IVY1, YIF1, YIP4 and YIP5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IVY1Q049343EBI-29509,EBI-35255
YIF1P538452EBI-29509,EBI-28230
YIP4P530932EBI-29509,EBI-24124

Protein-protein interaction databases

BioGridi35170. 200 interactions.
DIPiDIP-1735N.
IntActiP32939. 17 interactions.
MINTiMINT-385354.
STRINGi4932.YML001W.

Structurei

Secondary structure

1
208
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147Combined sources
Helixi21 – 3010Combined sources
Beta strandi46 – 505Combined sources
Beta strandi53 – 553Combined sources
Beta strandi58 – 636Combined sources
Helixi69 – 713Combined sources
Turni73 – 764Combined sources
Helixi77 – 793Combined sources
Beta strandi84 – 907Combined sources
Helixi94 – 985Combined sources
Helixi100 – 11112Combined sources
Turni116 – 1183Combined sources
Beta strandi121 – 1266Combined sources
Helixi132 – 1343Combined sources
Helixi139 – 14810Combined sources
Beta strandi154 – 1585Combined sources
Turni159 – 1624Combined sources
Helixi165 – 18016Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939.
SMRiP32939. Positions 3-182.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32939.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
InParanoidiP32939.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG7KSXMF.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV
60 70 80 90 100
TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI
110 120 130 140 150
KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG
160 170 180 190 200
DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD

GENNSCSC
Length:208
Mass (Da):23,043
Last modified:October 1, 1993 - v1
Checksum:i0E2B72BDA7F7CEE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRiA44334.
RefSeqiNP_013713.1. NM_001182356.1.

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W.
GeneIDi855012.
KEGGisce:YML001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRiA44334.
RefSeqiNP_013713.1. NM_001182356.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939.
SMRiP32939. Positions 3-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35170. 200 interactions.
DIPiDIP-1735N.
IntActiP32939. 17 interactions.
MINTiMINT-385354.
STRINGi4932.YML001W.

Proteomic databases

MaxQBiP32939.
PaxDbiP32939.
PeptideAtlasiP32939.
PRIDEiP32939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W.
GeneIDi855012.
KEGGisce:YML001W.

Organism-specific databases

CYGDiYML001w.
EuPathDBiFungiDB:YML001W.
SGDiS000004460. YPT7.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
InParanoidiP32939.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG7KSXMF.

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32939.
NextBioi978183.
PROiP32939.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)."
    Wichmann H., Hengst L., Gallwitz D.
    Cell 71:1131-1142(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Wada Y., Ohsumi M., Kawai A.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance."
    Haas A., Schegelmann D., Lazar T., Gallwitz D., Wickner W.
    EMBO J. 14:5258-5270(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
    Lazar T., Scheglmann D., Gallwitz D.
    Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IVY1.
  7. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYPT7_YEAST
AccessioniPrimary (citable) accession number: P32939
Secondary accession number(s): D6VZH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 24, 2015
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5530 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.