GTP-binding protein YPT7 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32939 (YPT7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein YPT7

Gene names

Name:	YPT7
Synonyms:	VAM4
Ordered Locus Names:	YML001W
ORF Names:	YM8270.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.
Subunit structure	Interacts with IVY1, YIF1, YIP4 and YIP5. Ref.6 Ref.7
Subcellular location	Vacuole.
Miscellaneous	Present with 5530 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Vacuole
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Traceable author statement. Source: SGD endocytosis Inferred from mutant phenotype Ref.1. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of vacuole fusion, non-autophagic Inferred from mutant phenotype Ref.5. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vacuole inheritance Inferred from direct assay Ref.5. Source: SGD
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.7 Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GDI1	P39958	4	EBI-29509,EBI-7517
IVY1	Q04934	3	EBI-29509,EBI-35255
YIF1	P53845	2	EBI-29509,EBI-28230
YIP4	P53093	2	EBI-29509,EBI-24124

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

GTP-binding protein YPT7

PRO_0000121320

Regions

Nucleotide binding

15 – 22

GTP By similarity

Nucleotide binding

64 – 68

GTP By similarity

Nucleotide binding

126 – 129

GTP By similarity

Motif

37 – 45

Effector region Probable

Amino acid modifications

Modified residue

208

Cysteine methyl ester By similarity

Lipidation

206

S-geranylgeranyl cysteine By similarity

Lipidation

208

S-geranylgeranyl cysteine By similarity

Secondary structure

208

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32939 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 0E2B72BDA7F7CEE5
Show »

FASTA

208

23,043

        10         20         30         40         50         60 
MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV TVDGDKVATM 

        70         80         90        100        110        120 
QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI KSWRDEFLVH ANVNSPETFP 

       130        140        150        160        170        180 
FVILGNKIDA EESKKIVSEK SAQELAKSLG DIPLFLTSAK NAINVDTAFE EIARSALQQN 

       190        200 
QADTEAFEDD YNDAINIRLD GENNSCSC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)." Wichmann H., Hengst L., Gallwitz D. Cell 71:1131-1142(1992) [PubMed: 1473149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Wada Y., Ohsumi M., Kawai A. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26786 / X2180-1A.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance." Haas A., Schegelmann D., Lazar T., Gallwitz D., Wickner W. EMBO J. 14:5258-5270(1995) [PubMed: 7489715] [Abstract] Cited for: CHARACTERIZATION.
[6]	"A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p." Lazar T., Scheglmann D., Gallwitz D. Eur. J. Cell Biol. 81:635-646(2002) [PubMed: 12553664] [Abstract] Cited for: INTERACTION WITH IVY1.
[7]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed: 11943201] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.

PIR

A44334.

RefSeq

NP_013713.1. NM_001182356.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KY2	X-ray	1.60	A	1-182	[»]
1KY3	X-ray	1.35	A	1-182	[»]

ProteinModelPortal

P32939.

SMR

P32939. Positions 3-182.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1735N.

IntAct

P32939. 19 interactions.

MINT

MINT-385354.

STRING

P32939.

Proteomic databases

PeptideAtlas

P32939.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YML001W; YML001W; YML001W.

GeneID

855012.

KEGG

sce:YML001W.

NMPDR

fig|4932.3.peg.4756.

Organism-specific databases

CYGD

YML001w.

SGD

S000004460. YPT7.

Phylogenomic databases

eggNOG

fuNOG06661.

GeneTree

EFGT00050000002458.

HOGENOM

HBG745225.

OMA

SPRDPEH.

OrthoDB

EOG48WGB7.

Gene expression databases

ArrayExpress

P32939.

Genevestigator

P32939.

GermOnline

YML001W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

K07897.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

978183.

Entry information

Entry name

YPT7_YEAST

Accession

Primary (citable) accession number: P32939
Secondary accession number(s): D6VZH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents