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P32939 (YPT7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein YPT7
Gene names
Name:YPT7
Synonyms:VAM4
Ordered Locus Names:YML001W
ORF Names:YM8270.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

Subunit structure

Interacts with IVY1, YIF1, YIP4 and YIP5. Ref.6 Ref.7

Subcellular location

Vacuole.

Miscellaneous

Present with 5530 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentVacuole
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Prenylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay PubMed 11210571. Source: GOC

Golgi to vacuole transport

Traceable author statement PubMed 9714809. Source: SGD

endocytosis

Inferred from mutant phenotype Ref.1. Source: SGD

piecemeal microautophagy of nucleus

Inferred from mutant phenotype PubMed 18701704. Source: SGD

protein localization to vacuole

Inferred from mutant phenotype PubMed 22593205. Source: SGD

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of vacuole fusion, non-autophagic

Inferred from mutant phenotype PubMed 11598008Ref.5. Source: SGD

retrograde transport, endosome to Golgi

Inferred from physical interaction PubMed 22593205. Source: SGD

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

vacuole inheritance

Inferred from direct assay Ref.5. Source: SGD

vesicle-mediated transport

Inferred from direct assay PubMed 9015302. Source: SGD

   Cellular_componentfungal-type vacuole

Inferred from direct assay PubMed 11853670. Source: SGD

fungal-type vacuole membrane

Inferred from direct assay PubMed 22593205. Source: SGD

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from direct assay PubMed 11210571. Source: SGD

protein binding

Inferred from physical interaction Ref.7Ref.6. Source: IntAct

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 208208GTP-binding protein YPT7
PRO_0000121320

Regions

Nucleotide binding15 – 228GTP By similarity
Nucleotide binding64 – 685GTP By similarity
Nucleotide binding126 – 1294GTP By similarity
Motif37 – 459Effector region Probable

Amino acid modifications

Modified residue2081Cysteine methyl ester By similarity
Lipidation2061S-geranylgeranyl cysteine By similarity
Lipidation2081S-geranylgeranyl cysteine By similarity

Secondary structure

................................... 208
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32939 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 0E2B72BDA7F7CEE5

FASTA20823,043
        10         20         30         40         50         60 
MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV TVDGDKVATM 

        70         80         90        100        110        120 
QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI KSWRDEFLVH ANVNSPETFP 

       130        140        150        160        170        180 
FVILGNKIDA EESKKIVSEK SAQELAKSLG DIPLFLTSAK NAINVDTAFE EIARSALQQN 

       190        200 
QADTEAFEDD YNDAINIRLD GENNSCSC 

« Hide

References

« Hide 'large scale' references
[1]"Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)."
Wichmann H., Hengst L., Gallwitz D.
Cell 71:1131-1142(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Wada Y., Ohsumi M., Kawai A.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26786 / X2180-1A.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance."
Haas A., Schegelmann D., Lazar T., Gallwitz D., Wickner W.
EMBO J. 14:5258-5270(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
Lazar T., Scheglmann D., Gallwitz D.
Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IVY1.
[7]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRA44334.
RefSeqNP_013713.1. NM_001182356.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
ProteinModelPortalP32939.
SMRP32939. Positions 3-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35170. 199 interactions.
DIPDIP-1735N.
IntActP32939. 17 interactions.
MINTMINT-385354.
STRING4932.YML001W.

Proteomic databases

MaxQBP32939.
PaxDbP32939.
PeptideAtlasP32939.
PRIDEP32939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML001W; YML001W; YML001W.
GeneID855012.
KEGGsce:YML001W.

Organism-specific databases

CYGDYML001w.
SGDS000004460. YPT7.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00700000104345.
HOGENOMHOG000233968.
KOK07897.
OMADYPDPIK.
OrthoDBEOG7KSXMF.

Enzyme and pathway databases

BioCycYEAST:G3O-32607-MONOMER.

Gene expression databases

GenevestigatorP32939.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32939.
NextBio978183.
PROP32939.

Entry information

Entry nameYPT7_YEAST
AccessionPrimary (citable) accession number: P32939
Secondary accession number(s): D6VZH4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references