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P32939

- YPT7_YEAST

UniProt

P32939 - YPT7_YEAST

Protein

GTP-binding protein YPT7

Gene

YPT7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi64 – 685GTPBy similarity
    Nucleotide bindingi126 – 1294GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. endocytosis Source: SGD
    2. Golgi to vacuole transport Source: SGD
    3. GTP catabolic process Source: GOC
    4. piecemeal microautophagy of nucleus Source: SGD
    5. protein localization to vacuole Source: SGD
    6. protein transport Source: UniProtKB-KW
    7. regulation of vacuole fusion, non-autophagic Source: SGD
    8. retrograde transport, endosome to Golgi Source: SGD
    9. small GTPase mediated signal transduction Source: InterPro
    10. vacuole inheritance Source: SGD
    11. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32607-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein YPT7
    Gene namesi
    Name:YPT7
    Synonyms:VAM4
    Ordered Locus Names:YML001W
    ORF Names:YM8270.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML001w.
    SGDiS000004460. YPT7.

    Subcellular locationi

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD
    2. fungal-type vacuole membrane Source: SGD

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 208208GTP-binding protein YPT7PRO_0000121320Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi206 – 2061S-geranylgeranyl cysteineBy similarity
    Modified residuei208 – 2081Cysteine methyl esterBy similarity
    Lipidationi208 – 2081S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP32939.
    PaxDbiP32939.
    PeptideAtlasiP32939.
    PRIDEiP32939.

    Expressioni

    Gene expression databases

    GenevestigatoriP32939.

    Interactioni

    Subunit structurei

    Interacts with IVY1, YIF1, YIP4 and YIP5.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IVY1Q049343EBI-29509,EBI-35255
    YIF1P538452EBI-29509,EBI-28230
    YIP4P530932EBI-29509,EBI-24124

    Protein-protein interaction databases

    BioGridi35170. 199 interactions.
    DIPiDIP-1735N.
    IntActiP32939. 17 interactions.
    MINTiMINT-385354.
    STRINGi4932.YML001W.

    Structurei

    Secondary structure

    1
    208
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 147
    Helixi21 – 3010
    Beta strandi46 – 505
    Beta strandi53 – 553
    Beta strandi58 – 636
    Helixi69 – 713
    Turni73 – 764
    Helixi77 – 793
    Beta strandi84 – 907
    Helixi94 – 985
    Helixi100 – 11112
    Turni116 – 1183
    Beta strandi121 – 1266
    Helixi132 – 1343
    Helixi139 – 14810
    Beta strandi154 – 1585
    Turni159 – 1624
    Helixi165 – 18016

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KY2X-ray1.60A1-182[»]
    1KY3X-ray1.35A1-182[»]
    4PHFX-ray1.95A1-182[»]
    4PHGX-ray1.90A1-182[»]
    4PHHX-ray2.35A/B/C/D1-182[»]
    ProteinModelPortaliP32939.
    SMRiP32939. Positions 3-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32939.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionCurated

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00700000104345.
    HOGENOMiHOG000233968.
    KOiK07897.
    OMAiDYPDPIK.
    OrthoDBiEOG7KSXMF.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32939-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV    50
    TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI 100
    KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG 150
    DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD 200
    GENNSCSC 208
    Length:208
    Mass (Da):23,043
    Last modified:October 1, 1993 - v1
    Checksum:i0E2B72BDA7F7CEE5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68144 Genomic DNA. Translation: CAA48244.1.
    D64114 Genomic DNA. Translation: BAA10973.1.
    Z48613 Genomic DNA. Translation: CAA88515.1.
    BK006946 Genomic DNA. Translation: DAA09898.1.
    PIRiA44334.
    RefSeqiNP_013713.1. NM_001182356.1.

    Genome annotation databases

    EnsemblFungiiYML001W; YML001W; YML001W.
    GeneIDi855012.
    KEGGisce:YML001W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68144 Genomic DNA. Translation: CAA48244.1 .
    D64114 Genomic DNA. Translation: BAA10973.1 .
    Z48613 Genomic DNA. Translation: CAA88515.1 .
    BK006946 Genomic DNA. Translation: DAA09898.1 .
    PIRi A44334.
    RefSeqi NP_013713.1. NM_001182356.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KY2 X-ray 1.60 A 1-182 [» ]
    1KY3 X-ray 1.35 A 1-182 [» ]
    4PHF X-ray 1.95 A 1-182 [» ]
    4PHG X-ray 1.90 A 1-182 [» ]
    4PHH X-ray 2.35 A/B/C/D 1-182 [» ]
    ProteinModelPortali P32939.
    SMRi P32939. Positions 3-182.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35170. 199 interactions.
    DIPi DIP-1735N.
    IntActi P32939. 17 interactions.
    MINTi MINT-385354.
    STRINGi 4932.YML001W.

    Proteomic databases

    MaxQBi P32939.
    PaxDbi P32939.
    PeptideAtlasi P32939.
    PRIDEi P32939.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML001W ; YML001W ; YML001W .
    GeneIDi 855012.
    KEGGi sce:YML001W.

    Organism-specific databases

    CYGDi YML001w.
    SGDi S000004460. YPT7.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00700000104345.
    HOGENOMi HOG000233968.
    KOi K07897.
    OMAi DYPDPIK.
    OrthoDBi EOG7KSXMF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32607-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32939.
    NextBioi 978183.
    PROi P32939.

    Gene expression databases

    Genevestigatori P32939.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)."
      Wichmann H., Hengst L., Gallwitz D.
      Cell 71:1131-1142(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Wada Y., Ohsumi M., Kawai A.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26786 / X2180-1A.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance."
      Haas A., Schegelmann D., Lazar T., Gallwitz D., Wickner W.
      EMBO J. 14:5258-5270(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
      Lazar T., Scheglmann D., Gallwitz D.
      Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IVY1.
    7. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYPT7_YEAST
    AccessioniPrimary (citable) accession number: P32939
    Secondary accession number(s): D6VZH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5530 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3