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Protein

GTP-binding protein YPT7

Gene

YPT7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi64 – 68GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • CVT pathway Source: SGD
  • endocytosis Source: SGD
  • macroautophagy Source: SGD
  • phagosome-lysosome fusion Source: GO_Central
  • piecemeal microautophagy of nucleus Source: SGD
  • protein localization to vacuole Source: SGD
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • retrograde transport, endosome to Golgi Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
  • vacuole inheritance Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT7
Gene namesi
Name:YPT7
Synonyms:VAM4
Ordered Locus Names:YML001W
ORF Names:YM8270.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML001W.
SGDiS000004460. YPT7.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • late endosome Source: GO_Central
  • lysosome Source: GO_Central
  • phagocytic vesicle Source: GO_Central
  • vacuole-mitochondrion membrane contact site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001213201 – 208GTP-binding protein YPT7Add BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Lipidationi206S-geranylgeranyl cysteineBy similarity1
Modified residuei208Cysteine methyl esterBy similarity1
Lipidationi208S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Methylation, Prenylation, Ubl conjugation

Proteomic databases

MaxQBiP32939.
PRIDEiP32939.
TopDownProteomicsiP32939.

PTM databases

iPTMnetiP32939.

Interactioni

Subunit structurei

Interacts with IVY1, YIF1, YIP4 and YIP5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IVY1Q049343EBI-29509,EBI-35255
YIF1P538452EBI-29509,EBI-28230
YIP4P530932EBI-29509,EBI-24124

Protein-protein interaction databases

BioGridi35170. 249 interactors.
DIPiDIP-1735N.
IntActiP32939. 17 interactors.
MINTiMINT-385354.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi21 – 30Combined sources10
Beta strandi46 – 50Combined sources5
Beta strandi53 – 55Combined sources3
Beta strandi58 – 63Combined sources6
Helixi69 – 71Combined sources3
Turni73 – 76Combined sources4
Helixi77 – 79Combined sources3
Beta strandi84 – 90Combined sources7
Helixi94 – 98Combined sources5
Helixi100 – 111Combined sources12
Turni116 – 118Combined sources3
Beta strandi121 – 126Combined sources6
Helixi132 – 134Combined sources3
Helixi139 – 148Combined sources10
Beta strandi154 – 158Combined sources5
Turni159 – 162Combined sources4
Helixi165 – 180Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939.
SMRiP32939.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32939.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
InParanoidiP32939.
KOiK07897.
OMAiAFEDDYN.
OrthoDBiEOG092C5MDH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV
60 70 80 90 100
TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI
110 120 130 140 150
KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG
160 170 180 190 200
DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD

GENNSCSC
Length:208
Mass (Da):23,043
Last modified:October 1, 1993 - v1
Checksum:i0E2B72BDA7F7CEE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRiA44334.
RefSeqiNP_013713.1. NM_001182356.1.

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W.
GeneIDi855012.
KEGGisce:YML001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRiA44334.
RefSeqiNP_013713.1. NM_001182356.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939.
SMRiP32939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35170. 249 interactors.
DIPiDIP-1735N.
IntActiP32939. 17 interactors.
MINTiMINT-385354.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

PTM databases

iPTMnetiP32939.

Proteomic databases

MaxQBiP32939.
PRIDEiP32939.
TopDownProteomicsiP32939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W.
GeneIDi855012.
KEGGisce:YML001W.

Organism-specific databases

EuPathDBiFungiDB:YML001W.
SGDiS000004460. YPT7.

Phylogenomic databases

GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
InParanoidiP32939.
KOiK07897.
OMAiAFEDDYN.
OrthoDBiEOG092C5MDH.

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP32939.
PROiP32939.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPT7_YEAST
AccessioniPrimary (citable) accession number: P32939
Secondary accession number(s): D6VZH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5530 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.