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P32939

- YPT7_YEAST

UniProt

P32939 - YPT7_YEAST

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Protein
GTP-binding protein YPT7
Gene
YPT7, VAM4, YML001W, YM8270.02
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Needed for homotypic vacuole fusion, the last step in the vacuole inheritance process.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTP By similarity
Nucleotide bindingi64 – 685GTP By similarity
Nucleotide bindingi126 – 1294GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. Golgi to vacuole transport Source: SGD
  3. endocytosis Source: SGD
  4. piecemeal microautophagy of nucleus Source: SGD
  5. protein localization to vacuole Source: SGD
  6. protein transport Source: UniProtKB-KW
  7. regulation of vacuole fusion, non-autophagic Source: SGD
  8. retrograde transport, endosome to Golgi Source: SGD
  9. small GTPase mediated signal transduction Source: InterPro
  10. vacuole inheritance Source: SGD
  11. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32607-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT7
Gene namesi
Name:YPT7
Synonyms:VAM4
Ordered Locus Names:YML001W
ORF Names:YM8270.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML001w.
SGDiS000004460. YPT7.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208GTP-binding protein YPT7
PRO_0000121320Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi206 – 2061S-geranylgeranyl cysteine By similarity
Modified residuei208 – 2081Cysteine methyl ester By similarity
Lipidationi208 – 2081S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP32939.
PaxDbiP32939.
PeptideAtlasiP32939.
PRIDEiP32939.

Expressioni

Gene expression databases

GenevestigatoriP32939.

Interactioni

Subunit structurei

Interacts with IVY1, YIF1, YIP4 and YIP5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IVY1Q049343EBI-29509,EBI-35255
YIF1P538452EBI-29509,EBI-28230
YIP4P530932EBI-29509,EBI-24124

Protein-protein interaction databases

BioGridi35170. 199 interactions.
DIPiDIP-1735N.
IntActiP32939. 17 interactions.
MINTiMINT-385354.
STRINGi4932.YML001W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147
Helixi21 – 3010
Beta strandi46 – 505
Beta strandi53 – 553
Beta strandi58 – 636
Helixi69 – 713
Turni73 – 764
Helixi77 – 793
Beta strandi84 – 907
Helixi94 – 985
Helixi100 – 11112
Turni116 – 1183
Beta strandi121 – 1266
Helixi132 – 1343
Helixi139 – 14810
Beta strandi154 – 1585
Turni159 – 1624
Helixi165 – 18016

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KY2X-ray1.60A1-182[»]
1KY3X-ray1.35A1-182[»]
4PHFX-ray1.95A1-182[»]
4PHGX-ray1.90A1-182[»]
4PHHX-ray2.35A/B/C/D1-182[»]
ProteinModelPortaliP32939.
SMRiP32939. Positions 3-182.

Miscellaneous databases

EvolutionaryTraceiP32939.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector region Inferred

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00700000104345.
HOGENOMiHOG000233968.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG7KSXMF.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32939-1 [UniParc]FASTAAdd to Basket

« Hide

MSSRKKNILK VIILGDSGVG KTSLMHRYVN DKYSQQYKAT IGADFLTKEV    50
TVDGDKVATM QVWDTAGQER FQSLGVAFYR GADCCVLVYD VTNASSFENI 100
KSWRDEFLVH ANVNSPETFP FVILGNKIDA EESKKIVSEK SAQELAKSLG 150
DIPLFLTSAK NAINVDTAFE EIARSALQQN QADTEAFEDD YNDAINIRLD 200
GENNSCSC 208
Length:208
Mass (Da):23,043
Last modified:October 1, 1993 - v1
Checksum:i0E2B72BDA7F7CEE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1.
D64114 Genomic DNA. Translation: BAA10973.1.
Z48613 Genomic DNA. Translation: CAA88515.1.
BK006946 Genomic DNA. Translation: DAA09898.1.
PIRiA44334.
RefSeqiNP_013713.1. NM_001182356.1.

Genome annotation databases

EnsemblFungiiYML001W; YML001W; YML001W.
GeneIDi855012.
KEGGisce:YML001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68144 Genomic DNA. Translation: CAA48244.1 .
D64114 Genomic DNA. Translation: BAA10973.1 .
Z48613 Genomic DNA. Translation: CAA88515.1 .
BK006946 Genomic DNA. Translation: DAA09898.1 .
PIRi A44334.
RefSeqi NP_013713.1. NM_001182356.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KY2 X-ray 1.60 A 1-182 [» ]
1KY3 X-ray 1.35 A 1-182 [» ]
4PHF X-ray 1.95 A 1-182 [» ]
4PHG X-ray 1.90 A 1-182 [» ]
4PHH X-ray 2.35 A/B/C/D 1-182 [» ]
ProteinModelPortali P32939.
SMRi P32939. Positions 3-182.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35170. 199 interactions.
DIPi DIP-1735N.
IntActi P32939. 17 interactions.
MINTi MINT-385354.
STRINGi 4932.YML001W.

Proteomic databases

MaxQBi P32939.
PaxDbi P32939.
PeptideAtlasi P32939.
PRIDEi P32939.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML001W ; YML001W ; YML001W .
GeneIDi 855012.
KEGGi sce:YML001W.

Organism-specific databases

CYGDi YML001w.
SGDi S000004460. YPT7.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00700000104345.
HOGENOMi HOG000233968.
KOi K07897.
OMAi DYPDPIK.
OrthoDBi EOG7KSXMF.

Enzyme and pathway databases

BioCyci YEAST:G3O-32607-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32939.
NextBioi 978183.
PROi P32939.

Gene expression databases

Genevestigatori P32939.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)."
    Wichmann H., Hengst L., Gallwitz D.
    Cell 71:1131-1142(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Wada Y., Ohsumi M., Kawai A.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance."
    Haas A., Schegelmann D., Lazar T., Gallwitz D., Wickner W.
    EMBO J. 14:5258-5270(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
    Lazar T., Scheglmann D., Gallwitz D.
    Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IVY1.
  7. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYPT7_YEAST
AccessioniPrimary (citable) accession number: P32939
Secondary accession number(s): D6VZH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5530 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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