P32936 (IAAB_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase/trypsin inhibitor CMb

Alternative name(s):
Chloroform/methanol-soluble protein CMb

Gene names

Name:

IAT2

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a complex with inhibitory activity, but CMb is inactive as a separate subunit.
Subunit structure	Heterotetramer of one CMa, one CMb and two CMd chains.
Subcellular location	Secreted.
Tissue specificity	Endosperm.
Post-translational modification	Five disulfide bonds are present Probable, which are essential for the inhibitor activity. Exists both in a glycosylated and in a unglycosylated form. The glycosylated form is a potent allergen.
Allergenic properties	Causes an allergic reaction in human. Involved in baker's asthma.
Sequence similarities	Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Alpha-amylase inhibitor Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.2 Ref.3
	Chain	25 – 149	125	Alpha-amylase/trypsin inhibitor CMb		PRO_0000014351
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	30	1	C → D AA sequence Ref.3
	Sequence conflict	33	1	W → P AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P32936 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: A33E42B146A42847	FASTA	149	16,526
	10 20 30 40 50 60 MASKSSCDLL LAAVLVSIFA AVAAVGSEDC TPWTATPITP LPSCRDYVEQ QACRIETPGP 70 80 90 100 110 120 PYLAKQQCCG ELANIPQQCR CQALRFFMGR KSRPDQSGLM ELPGCPREVQ MDFVRILVTP 130 140 GFCNLTTVHN TPYCLAMDEW QWNRQFCSS

References

[1]	"Cloning of cDNA, expression, and chromosomal location of genes encoding the three types of subunits of the barley tetrameric inhibitor of insect alpha-amylase." Medina-Alcazar J., Hueros G., Carbonero P. Plant Mol. Biol. 23:535-542(1993) [PubMed: 8219088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Abyssinian. Tissue: Endosperm.
[2]	"New alpha-amylase and trypsin inhibitors among the CM-proteins of barley (Hordeum vulgare)." Barber D., Sanchez-Monge R., Mendez E., Lazaro A., Garcia-Olmedo F., Salcedo G. Biochim. Biophys. Acta 869:115-118(1986) [PubMed: 3484638] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-53.
[3]	"Separation of acidic barley endosperm proteins by two-dimensional electrophoresis." Flengsrud R. Electrophoresis 14:1060-1066(1993) [PubMed: 8125056] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-33. Strain: cv. H354-295-2-5. Tissue: Starchy endosperm.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69938 mRNA. Translation: CAA49556.1.
PIR	S78524.
3D structure databases
ProteinModelPortal	P32936.
ModBase	Search...
Protein family/group databases
Allergome	418. Hor v 15.
MEROPS	I06.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P32936.
Gene expression databases
Genevestigator	P32936.
Family and domain databases
InterPro	IPR006106. Allergen/soft/tryp_amyl_inhib. IPR006105. Allergen/tryp_amyl_inhib_CS. IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00808. AMLASEINHBTR.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00426. CEREAL_TRYP_AMYL_INH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAAB_HORVU

Accession

Primary (citable) accession number: P32936

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program