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Protein

Nucleoprotein

Gene

N

Organism
Avian infectious bronchitis virus (strain Gray) (IBV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:6
OrganismiAvian infectious bronchitis virus (strain Gray) (IBV)
Taxonomic identifieri31624 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeGammacoronavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409NucleoproteinPRO_0000105981Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901Phosphoserine; by hostBy similarity
Modified residuei192 – 1921Phosphoserine; by hostBy similarity
Disulfide bondi320 ↔ 323By similarity
Modified residuei378 – 3781Phosphothreonine; by hostBy similarity
Modified residuei379 – 3791Phosphoserine; by hostBy similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein.

Structurei

Secondary structure

1
409
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 435Combined sources
Beta strandi51 – 533Combined sources
Helixi64 – 663Combined sources
Beta strandi68 – 7811Combined sources
Beta strandi81 – 833Combined sources
Beta strandi85 – 9511Combined sources
Turni100 – 1034Combined sources
Beta strandi113 – 1175Combined sources
Turni134 – 1363Combined sources
Helixi227 – 2293Combined sources
Helixi239 – 2424Combined sources
Beta strandi247 – 2493Combined sources
Helixi256 – 2616Combined sources
Helixi262 – 2643Combined sources
Helixi266 – 2727Combined sources
Helixi278 – 2847Combined sources
Beta strandi285 – 2928Combined sources
Beta strandi295 – 30713Combined sources
Helixi313 – 32311Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GE7X-ray2.00A/B226-333[»]
2GE8X-ray2.20A/B/C/D/F/G/I/J220-333[»]
2GECX-ray1.30A/B22-160[»]
ProteinModelPortaliP32923.
SMRiP32923. Positions 22-160, 220-332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32923.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 160132RNA-bindingAdd
BLAST
Regioni226 – 333108DimerizationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi165 – 19026Ser-richAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P32923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKATGKT DAPAPVIKLG GPRPPKVGSS GNASWFQAIK AKKLNSPQPK
60 70 80 90 100
FEGSGVPDNE NFKTSQQHGY WRRQARFKPG KGRRKPVPDA WYFYYTGTGP
110 120 130 140 150
AADLNWGDSQ DGIVWVAAKG ADVKSRSNQG TRDPDKFDQY PLRFSDGGPD
160 170 180 190 200
GNFRWDFIPL NRGRSGRSTA ASSAASSRPP SREGSRGRRS GSEDDLIARA
210 220 230 240 250
AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPGYKVDQ VFGPRTKGKE
260 270 280 290 300
GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQPDGLHLKF
310 320 330 340 350
EFTTVVPRDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRT
360 370 380 390 400
SSPAPRQQRL KKEKRPKKQD DEVDKALTSD EERNNAQLEF DDEPKVINWG

DSALGENEL
Length:409
Mass (Da):45,249
Last modified:June 1, 1994 - v2
Checksum:i5C15F07A0A60BE80
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 24321AHRRY…DQVFG → VIAGIASALFHLVIRLIKFL V (PubMed:1966426).CuratedAdd
BLAST
Sequence conflicti245 – 2451R → G (PubMed:1966426).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55229 mRNA. No translation available.
S48137 Genomic RNA. Translation: AAB24054.1.
M85245 Genomic RNA. Translation: AAA91856.1.
PIRiA43574. VHIHG1.
A48559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55229 mRNA. No translation available.
S48137 Genomic RNA. Translation: AAB24054.1.
M85245 Genomic RNA. Translation: AAA91856.1.
PIRiA43574. VHIHG1.
A48559.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GE7X-ray2.00A/B226-333[»]
2GE8X-ray2.20A/B/C/D/F/G/I/J220-333[»]
2GECX-ray1.30A/B22-160[»]
ProteinModelPortaliP32923.
SMRiP32923. Positions 22-160, 220-332.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP32923.

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence comparisons of the 3' end of the genomes of five strains of avian infectious bronchitis virus."
    Collisson E.W., Williams A.K., Vonder Haar R., Li W., Sneed L.W.
    Adv. Exp. Med. Biol. 276:373-377(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses."
    Williams A.K., Wang L., Sneed L.W., Collisson E.W.
    Virus Res. 25:213-222(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Nucleocapsid gene sequence analysis of several strains of infectious bronchitis virus describing the evolutionary relationship of IBV within the coronaviridae family."
    Williams A.K., Wang L., Sneed L.W., Collisson E.W.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "X-ray structures of the N- and C-terminal domains of a coronavirus nucleocapsid protein: implications for nucleocapsid formation."
    Jayaram H., Fan H., Bowman B.R., Ooi A., Jayaram J., Collisson E.W., Lescar J., Prasad B.V.V.
    J. Virol. 80:6612-6620(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 22-160, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 226-333, RNA-BINDING, DIMERIZATION DOMAIN.

Entry informationi

Entry nameiNCAP_IBVG
AccessioniPrimary (citable) accession number: P32923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.