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Protein

Nucleoprotein

Gene

N

Organism
Avian infectious bronchitis virus (strain Gray) (IBV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:6
OrganismiAvian infectious bronchitis virus (strain Gray) (IBV)
Taxonomic identifieri31624 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeGammacoronavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059811 – 409NucleoproteinAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Phosphoserine; by hostBy similarity1
Modified residuei192Phosphoserine; by hostBy similarity1
Disulfide bondi320 ↔ 323By similarity
Modified residuei378Phosphothreonine; by hostBy similarity1
Modified residuei379Phosphoserine; by hostBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein.

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 43Combined sources5
Beta strandi51 – 53Combined sources3
Helixi64 – 66Combined sources3
Beta strandi68 – 78Combined sources11
Beta strandi81 – 83Combined sources3
Beta strandi85 – 95Combined sources11
Turni100 – 103Combined sources4
Beta strandi113 – 117Combined sources5
Turni134 – 136Combined sources3
Helixi227 – 229Combined sources3
Helixi239 – 242Combined sources4
Beta strandi247 – 249Combined sources3
Helixi256 – 261Combined sources6
Helixi262 – 264Combined sources3
Helixi266 – 272Combined sources7
Helixi278 – 284Combined sources7
Beta strandi285 – 292Combined sources8
Beta strandi295 – 307Combined sources13
Helixi313 – 323Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GE7X-ray2.00A/B226-333[»]
2GE8X-ray2.20A/B/C/D/F/G/I/J220-333[»]
2GECX-ray1.30A/B22-160[»]
ProteinModelPortaliP32923.
SMRiP32923.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32923.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 160RNA-bindingAdd BLAST132
Regioni226 – 333DimerizationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi165 – 190Ser-richAdd BLAST26

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P32923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKATGKT DAPAPVIKLG GPRPPKVGSS GNASWFQAIK AKKLNSPQPK
60 70 80 90 100
FEGSGVPDNE NFKTSQQHGY WRRQARFKPG KGRRKPVPDA WYFYYTGTGP
110 120 130 140 150
AADLNWGDSQ DGIVWVAAKG ADVKSRSNQG TRDPDKFDQY PLRFSDGGPD
160 170 180 190 200
GNFRWDFIPL NRGRSGRSTA ASSAASSRPP SREGSRGRRS GSEDDLIARA
210 220 230 240 250
AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPGYKVDQ VFGPRTKGKE
260 270 280 290 300
GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQPDGLHLKF
310 320 330 340 350
EFTTVVPRDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRT
360 370 380 390 400
SSPAPRQQRL KKEKRPKKQD DEVDKALTSD EERNNAQLEF DDEPKVINWG

DSALGENEL
Length:409
Mass (Da):45,249
Last modified:June 1, 1994 - v2
Checksum:i5C15F07A0A60BE80
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223 – 243AHRRY…DQVFG → VIAGIASALFHLVIRLIKFL V (PubMed:1966426).CuratedAdd BLAST21
Sequence conflicti245R → G (PubMed:1966426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55229 mRNA. No translation available.
S48137 Genomic RNA. Translation: AAB24054.1.
M85245 Genomic RNA. Translation: AAA91856.1.
PIRiA43574. VHIHG1.
A48559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55229 mRNA. No translation available.
S48137 Genomic RNA. Translation: AAB24054.1.
M85245 Genomic RNA. Translation: AAA91856.1.
PIRiA43574. VHIHG1.
A48559.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GE7X-ray2.00A/B226-333[»]
2GE8X-ray2.20A/B/C/D/F/G/I/J220-333[»]
2GECX-ray1.30A/B22-160[»]
ProteinModelPortaliP32923.
SMRiP32923.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP32923.

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_IBVG
AccessioniPrimary (citable) accession number: P32923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.