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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

Wars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).By similarity

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Angiogenesis, Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.2. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Cleaved into the following 2 chains:
Gene namesi
Name:Wars
Synonyms:Wrs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:104630. Wars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Tryptophan--tRNA ligase, cytoplasmicPRO_0000136739Add
BLAST
Chaini75 – 481407T1-TrpRSBy similarityPRO_0000386463Add
BLAST
Chaini98 – 481384T2-TrpRSBy similarityPRO_0000386464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei158 – 1581N6-succinyllysineCombined sources
Modified residuei355 – 3551PhosphoserineBy similarity

Post-translational modificationi

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32921.
PaxDbiP32921.
PeptideAtlasiP32921.
PRIDEiP32921.

PTM databases

iPTMnetiP32921.
PhosphoSiteiP32921.
SwissPalmiP32921.

Expressioni

Tissue specificityi

Isoform 2 is widely expressed, isoform 1 is found only in embryonic stem cells.

Gene expression databases

BgeeiENSMUSG00000021266.
CleanExiMM_WARS.
ExpressionAtlasiP32921. baseline and differential.
GenevisibleiP32921. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with oxidized form of GAPDH (By similarity).By similarity

Protein-protein interaction databases

BioGridi204543. 2 interactions.
IntActiP32921. 2 interactions.
MINTiMINT-4128539.
STRINGi10090.ENSMUSP00000124625.

Structurei

3D structure databases

ProteinModelPortaliP32921.
SMRiP32921. Positions 100-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 6857WHEP-TRSPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi168 – 17710"HIGH" region
Motifi353 – 3575"KMSKS" region

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
HOVERGENiHBG002325.
InParanoidiP32921.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG091G09U9.
PhylomeDBiP32921.
TreeFamiTF105669.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR002306. Trp-tRNA-ligase.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P32921-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADMPSGESC TSPLELFNSI ATQGELVRSL KAGNAPKDEI DSAVKMLLSL
60 70 80 90 100
KMSYKAAMGE EYKAGCPPGN PTAGRNCDSD ATKASEDFVD PWTVRTSSAK
110 120 130 140 150
GIDYDKLIVQ FGSSKIDKEL INRIERATGQ RPHRFLRRGI FFSHRDMNQI
160 170 180 190 200
LDAYENKKPF YLYTGRGPSS EAMHLGHLVP FIFTKWLQDV FNVPLVIQMS
210 220 230 240 250
DDEKYLWKDL TLEQAYSYTV ENAKDIIACG FDINKTFIFS DLEYMGQSPG
260 270 280 290 300
FYRNVVKIQK HVTFNQVKGI FGFTDSDCIG KISFPAVQAA PSFSNSFPKI
310 320 330 340 350
FRDRTDIQCL IPCAIDQDPY FRMTRDVAPR IGHPKPALLH STFFPALQGA
360 370 380 390 400
QTKMSASDPN SSIFLTDTAK QIKSKVNKHA FSGGRDTVEE HRQFGGNCEV
410 420 430 440 450
DVSFMYLTFF LEDDDRLEQI RKDYTSGAML TGELKKTLID VLQPLIAEHQ
460 470 480
ARRKAVTEET VKEFMTPRQL SFHFQCFCFD T
Length:481
Mass (Da):54,358
Last modified:August 31, 2004 - v2
Checksum:i39B55989AE895346
GO
Isoform 2 (identifier: P32921-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     476-481: Missing.

Show »
Length:475
Mass (Da):53,641
Checksum:iC3467FE85521DE4C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521M → V in AAH03450 (PubMed:15489334).Curated
Sequence conflicti94 – 941V → A in AAH46232 (PubMed:15489334).Curated
Sequence conflicti111 – 1111F → P in CAA49347 (PubMed:7932716).Curated
Sequence conflicti111 – 1111F → P in CAA49348 (PubMed:7932716).Curated
Sequence conflicti188 – 1881Q → E in CAA49347 (PubMed:7932716).Curated
Sequence conflicti188 – 1881Q → E in CAA49348 (PubMed:7932716).Curated
Sequence conflicti282 – 2821I → S in CAA49347 (PubMed:7932716).Curated
Sequence conflicti282 – 2821I → S in CAA49348 (PubMed:7932716).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei476 – 4816Missing in isoform 2. 2 PublicationsVSP_006313

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69656 Genomic DNA. Translation: CAA49347.1.
X69657 Genomic DNA. Translation: CAA49348.1.
AK004541 mRNA. Translation: BAB23357.1.
BC003450 mRNA. Translation: AAH03450.1.
BC046232 mRNA. Translation: AAH46232.1.
CCDSiCCDS26166.1. [P32921-1]
CCDS49171.1. [P32921-2]
PIRiS50053.
RefSeqiNP_001157786.1. NM_001164314.1. [P32921-2]
NP_001157960.1. NM_001164488.1. [P32921-2]
NP_035840.3. NM_011710.3. [P32921-1]
XP_006515882.1. XM_006515819.1. [P32921-1]
UniGeneiMm.38433.

Genome annotation databases

EnsembliENSMUST00000109848; ENSMUSP00000105474; ENSMUSG00000021266. [P32921-2]
ENSMUST00000161154; ENSMUSP00000124625; ENSMUSG00000021266. [P32921-1]
ENSMUST00000161410; ENSMUSP00000125320; ENSMUSG00000021266. [P32921-2]
GeneIDi22375.
KEGGimmu:22375.
UCSCiuc007pag.2. mouse. [P32921-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69656 Genomic DNA. Translation: CAA49347.1.
X69657 Genomic DNA. Translation: CAA49348.1.
AK004541 mRNA. Translation: BAB23357.1.
BC003450 mRNA. Translation: AAH03450.1.
BC046232 mRNA. Translation: AAH46232.1.
CCDSiCCDS26166.1. [P32921-1]
CCDS49171.1. [P32921-2]
PIRiS50053.
RefSeqiNP_001157786.1. NM_001164314.1. [P32921-2]
NP_001157960.1. NM_001164488.1. [P32921-2]
NP_035840.3. NM_011710.3. [P32921-1]
XP_006515882.1. XM_006515819.1. [P32921-1]
UniGeneiMm.38433.

3D structure databases

ProteinModelPortaliP32921.
SMRiP32921. Positions 100-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204543. 2 interactions.
IntActiP32921. 2 interactions.
MINTiMINT-4128539.
STRINGi10090.ENSMUSP00000124625.

PTM databases

iPTMnetiP32921.
PhosphoSiteiP32921.
SwissPalmiP32921.

Proteomic databases

MaxQBiP32921.
PaxDbiP32921.
PeptideAtlasiP32921.
PRIDEiP32921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109848; ENSMUSP00000105474; ENSMUSG00000021266. [P32921-2]
ENSMUST00000161154; ENSMUSP00000124625; ENSMUSG00000021266. [P32921-1]
ENSMUST00000161410; ENSMUSP00000125320; ENSMUSG00000021266. [P32921-2]
GeneIDi22375.
KEGGimmu:22375.
UCSCiuc007pag.2. mouse. [P32921-1]

Organism-specific databases

CTDi7453.
MGIiMGI:104630. Wars.

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
HOVERGENiHBG002325.
InParanoidiP32921.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG091G09U9.
PhylomeDBiP32921.
TreeFamiTF105669.

Enzyme and pathway databases

BRENDAi6.1.1.2. 3474.

Miscellaneous databases

ChiTaRSiWars. mouse.
PROiP32921.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021266.
CleanExiMM_WARS.
ExpressionAtlasiP32921. baseline and differential.
GenevisibleiP32921. MM.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR002306. Trp-tRNA-ligase.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYWC_MOUSE
AccessioniPrimary (citable) accession number: P32921
Secondary accession number(s): Q80ZY4, Q99J58, Q9DC65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 31, 2004
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.