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P32917 (STE5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein STE5
Gene names
Name:STE5
Synonyms:NUL3
Ordered Locus Names:YDR103W
ORF Names:YD8557.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length917 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone.

Subcellular location

Cytoplasm.

Post-translational modification

May be regulated at the phosphorylation level, and by the mating type of the cell and depends on an intact pheromone-response pathway.

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.

Sequence similarities

To yeast FAR1.

Ontologies

Keywords
   Biological processPheromone response
   Cellular componentCytoplasm
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processinvasive growth in response to glucose limitation

Inferred from mutant phenotype PubMed 15192700. Source: SGD

negative regulation of MAPK cascade

Inferred from mutant phenotype PubMed 16424299. Source: SGD

pheromone-dependent signal transduction involved in conjugation with cellular fusion

Inferred from direct assay PubMed 8062390. Source: SGD

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 19303851. Source: SGD

positive regulation of signal transduction

Inferred from physical interaction PubMed 7851759PubMed 8062390. Source: GOC

regulation of transposition, RNA-mediated

Inferred from mutant phenotype PubMed 9566871. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 11781566. Source: SGD

mating projection tip

Inferred from direct assay PubMed 11781566PubMed 17952059. Source: SGD

nucleus

Inferred from direct assay PubMed 9732267. Source: SGD

plasma membrane

Inferred from direct assay PubMed 9732267. Source: SGD

   Molecular_functionMAP-kinase scaffold activity

Inferred from physical interaction PubMed 7851759PubMed 8062390. Source: SGD

phosphatidylinositol-4,5-bisphosphate binding

Inferred from direct assay PubMed 16847350. Source: SGD

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CAGL0J04290gQ6FPF22EBI-18373,EBI-8783845From a different organism.
CEK1Q5A1D32EBI-18373,EBI-8783371From a different organism.
CEK2Q5A2812EBI-18373,EBI-8785242From a different organism.
FUS1P117102EBI-18373,EBI-7179
FUS3P1689210EBI-18373,EBI-7193
KLLA0E10539gQ6CNR32EBI-18373,EBI-8785667From a different organism.
mpkBC8V7D12EBI-18373,EBI-8783425From a different organism.
STE11P235617EBI-18373,EBI-18259
STE4P188512EBI-18373,EBI-7390
STE7P067848EBI-18373,EBI-18389

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 917917Protein STE5
PRO_0000072266

Regions

Compositional bias775 – 876102Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue3291Phosphoserine Ref.8

Experimental info

Sequence conflict331 – 3322LG → W in AAA35108. Ref.2
Sequence conflict341 – 3433NSI → TLS in AAA35108. Ref.2
Sequence conflict8211A → R in BAA02301. Ref.1

Secondary structure

........................................... 917
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32917 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 0435BDA0196B2D6F

FASTA917102,727
        10         20         30         40         50         60 
MMETPTDNIV SPFHNFGSST QYSGTLSRTP NQIIELEKPS TLSPLSRGKK WTEKLARFQR 

        70         80         90        100        110        120 
SSAKKKRFSP SPISSSTFSF SPKSRVTSSN SSGNEDGNLM NTPSTVSTDY LPQHPHRTSS 

       130        140        150        160        170        180 
LPRPNSNLFH ASNSNLSRAN EPPRAENLSD NIPPKVAPFG YPIQRTSIKK SFLNASCTLC 

       190        200        210        220        230        240 
DEPISNRRKG EKIIELACGH LSHQECLIIS FGTTSKADVR ALFPFCTKCK KDTNKAVQCI 

       250        260        270        280        290        300 
PENDELKDIL ISDFLIHKIP DSELSITPQS RFPPYSPLLP PFGLSYTPVE RQTIYSQAPS 

       310        320        330        340        350        360 
LNPNLILAAP PKERNQIPQK KSNYTFLHSP LGHRRIPSGA NSILADTSVA LSANDSISAV 

       370        380        390        400        410        420 
SNSVRAKDDE TKTTLPLLRS YFIQILLNNF QEELQDWRID GDYGLLRLVD KLMISKDGQR 

       430        440        450        460        470        480 
YIQCWCFLFE DAFVIAEVDN DVDVLEIRLK NLEVFTPIAN LRMTTLEASV LKCTLNKQHC 

       490        500        510        520        530        540 
ADLSDLYIVQ NINSDESTTV QKWISGILNQ DFVFNEDNIT STLPILPIIK NFSKDVGNGR 

       550        560        570        580        590        600 
HETSTFLGLI NPNKVVEVGN VHDNDTVIIR RGFTLNSGEC SRQSTVDSIQ SVLTTISSIL 

       610        620        630        640        650        660 
SLKREKPDNL AIILQIDFTK LKEEDSLIVV YNSLKALTIK FARLQFCFVD RNNYVLDYGS 

       670        680        690        700        710        720 
VLHKIDSLDS ISNLKSKSSS TQFSPIWLKN TLYPENIHEH LGIVAVSNSN MEAKKSILFQ 

       730        740        750        760        770        780 
DYRCFTSFGR RRPNELKIKV GYLNVDYSDK IDELVEASSW TFVLETLCYS FGLSFDEHDD 

       790        800        810        820        830        840 
DDEEDNDDST DNELDNSSGS LSDAESTTTI HIDSPFDNEN ATANMVNDRN LLTEGEHSNI 

       850        860        870        880        890        900 
ENLETVASSV QPALIPNIRF SLHSEEEGTN ENENENDMPV LLLSDMDKGI DGITRRSSFS 

       910 
SLIESGNNNC PLHMDYI 

« Hide

References

« Hide 'large scale' references
[1]"Function of the ste signal transduction pathway for mating pheromones sustains MAT alpha 1 transcription in Saccharomyces cerevisiae."
Mukai Y., Harashima S., Oshima Y.
Mol. Cell. Biol. 13:2050-2060(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants."
Perlman R., Yablonski D., Simchen G., Levitzki A.
Proc. Natl. Acad. Sci. U.S.A. 90:5474-5478(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence and characterization of the STE5 gene required for signal transduction and mating in Saccharomyces cerevisiae."
Mackay V.L., Mathewes S., Bell L., O'Hara P.J.
Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[4]"Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1."
Leberer E., Dignard D., Harcus D., Hougan L., Whiteway M., Thomas D.Y.
Mol. Gen. Genet. 241:241-254(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBY939.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12917 Genomic DNA. Translation: BAA02301.1.
L01620 Genomic DNA. Translation: AAA35108.1.
L23856 Genomic DNA. Translation: AAA35115.1.
L07865 Unassigned DNA. Translation: AAA16896.1.
Z47746 Genomic DNA. Translation: CAA87679.1.
BK006938 Genomic DNA. Translation: DAA11949.1.
PIRS51254.
RefSeqNP_010388.1. NM_001180411.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KGNNMR-A44-67[»]
2L4UNMR-A44-67[»]
3FZEX-ray1.60A593-786[»]
4F2HX-ray3.19A583-787[»]
ProteinModelPortalP32917.
SMRP32917. Positions 584-774.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32160. 47 interactions.
DIPDIP-858N.
IntActP32917. 20 interactions.
MINTMINT-1174440.
STRING4932.YDR103W.

Proteomic databases

PaxDbP32917.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR103W; YDR103W; YDR103W.
GeneID851680.
KEGGsce:YDR103W.

Organism-specific databases

CYGDYDR103w.
SGDS000002510. STE5.

Phylogenomic databases

eggNOGNOG44001.
HOGENOMHOG000065959.
KOK11239.
OMATLCDEPI.
OrthoDBEOG7712HK.

Enzyme and pathway databases

BioCycYEAST:G3O-29705-MONOMER.

Gene expression databases

GenevestigatorP32917.

Family and domain databases

InterProIPR021651. Scaffold_Ste5_Fus5-binding.
IPR021106. Ste5_Fus3-bd.
IPR001841. Znf_RING.
[Graphical view]
PfamPF11610. Ste5. 1 hit.
PF12194. Ste5_C. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32917.
NextBio969315.

Entry information

Entry nameSTE5_YEAST
AccessionPrimary (citable) accession number: P32917
Secondary accession number(s): D6VS89
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references