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Protein

Protein STE5

Gene

STE5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone.

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: SGD

GO - Biological processi

  • invasive growth in response to glucose limitation Source: SGD
  • negative regulation of MAPK cascade Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • positive regulation of protein phosphorylation Source: SGD
  • regulation of transposition, RNA-mediated Source: SGD

Keywordsi

Biological processPheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-29705-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STE5
Gene namesi
Name:STE5
Synonyms:NUL3
Ordered Locus Names:YDR103W
ORF Names:YD8557.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR103W.
SGDiS000002510. STE5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722661 – 917Protein STE5Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei329PhosphoserineCombined sources1

Post-translational modificationi

May be regulated at the phosphorylation level, and by the mating type of the cell and depends on an intact pheromone-response pathway.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32917.
PRIDEiP32917.

PTM databases

iPTMnetiP32917.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi32160. 52 interactors.
DIPiDIP-858N.
IntActiP32917. 23 interactors.
MINTiMINT-1174440.
STRINGi4932.YDR103W.

Structurei

Secondary structure

1917
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 48Combined sources4
Helixi49 – 51Combined sources3
Turni52 – 54Combined sources3
Helixi55 – 58Combined sources4
Helixi59 – 63Combined sources5
Turni64 – 66Combined sources3
Helixi593 – 604Combined sources12
Beta strandi608 – 616Combined sources9
Helixi618 – 620Combined sources3
Beta strandi622 – 624Combined sources3
Helixi625 – 640Combined sources16
Beta strandi645 – 650Combined sources6
Beta strandi653 – 660Combined sources8
Helixi661 – 664Combined sources4
Helixi668 – 672Combined sources5
Helixi673 – 676Combined sources4
Helixi685 – 692Combined sources8
Beta strandi701 – 707Combined sources7
Turni713 – 715Combined sources3
Helixi717 – 719Combined sources3
Helixi723 – 725Combined sources3
Beta strandi735 – 741Combined sources7
Turni748 – 750Combined sources3
Beta strandi755 – 758Combined sources4
Helixi760 – 770Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KGNNMR-A44-67[»]
2L4UNMR-A44-67[»]
3FZEX-ray1.60A593-786[»]
4F2HX-ray3.19A583-787[»]
ProteinModelPortaliP32917.
SMRiP32917.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32917.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi775 – 876Asp/Glu-rich (acidic)Add BLAST102

Sequence similaritiesi

To yeast FAR1.Curated

Phylogenomic databases

HOGENOMiHOG000065959.
InParanoidiP32917.
KOiK11239.
OMAiTLCDEPI.
OrthoDBiEOG092C0N3E.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR021651. Ste5_Fus-binding.
IPR021106. Ste5_Fus3-bd_dom.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF11610. Ste5. 1 hit.
PF12194. Ste5_C. 1 hit.

Sequencei

Sequence statusi: Complete.

P32917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMETPTDNIV SPFHNFGSST QYSGTLSRTP NQIIELEKPS TLSPLSRGKK
60 70 80 90 100
WTEKLARFQR SSAKKKRFSP SPISSSTFSF SPKSRVTSSN SSGNEDGNLM
110 120 130 140 150
NTPSTVSTDY LPQHPHRTSS LPRPNSNLFH ASNSNLSRAN EPPRAENLSD
160 170 180 190 200
NIPPKVAPFG YPIQRTSIKK SFLNASCTLC DEPISNRRKG EKIIELACGH
210 220 230 240 250
LSHQECLIIS FGTTSKADVR ALFPFCTKCK KDTNKAVQCI PENDELKDIL
260 270 280 290 300
ISDFLIHKIP DSELSITPQS RFPPYSPLLP PFGLSYTPVE RQTIYSQAPS
310 320 330 340 350
LNPNLILAAP PKERNQIPQK KSNYTFLHSP LGHRRIPSGA NSILADTSVA
360 370 380 390 400
LSANDSISAV SNSVRAKDDE TKTTLPLLRS YFIQILLNNF QEELQDWRID
410 420 430 440 450
GDYGLLRLVD KLMISKDGQR YIQCWCFLFE DAFVIAEVDN DVDVLEIRLK
460 470 480 490 500
NLEVFTPIAN LRMTTLEASV LKCTLNKQHC ADLSDLYIVQ NINSDESTTV
510 520 530 540 550
QKWISGILNQ DFVFNEDNIT STLPILPIIK NFSKDVGNGR HETSTFLGLI
560 570 580 590 600
NPNKVVEVGN VHDNDTVIIR RGFTLNSGEC SRQSTVDSIQ SVLTTISSIL
610 620 630 640 650
SLKREKPDNL AIILQIDFTK LKEEDSLIVV YNSLKALTIK FARLQFCFVD
660 670 680 690 700
RNNYVLDYGS VLHKIDSLDS ISNLKSKSSS TQFSPIWLKN TLYPENIHEH
710 720 730 740 750
LGIVAVSNSN MEAKKSILFQ DYRCFTSFGR RRPNELKIKV GYLNVDYSDK
760 770 780 790 800
IDELVEASSW TFVLETLCYS FGLSFDEHDD DDEEDNDDST DNELDNSSGS
810 820 830 840 850
LSDAESTTTI HIDSPFDNEN ATANMVNDRN LLTEGEHSNI ENLETVASSV
860 870 880 890 900
QPALIPNIRF SLHSEEEGTN ENENENDMPV LLLSDMDKGI DGITRRSSFS
910
SLIESGNNNC PLHMDYI
Length:917
Mass (Da):102,727
Last modified:February 1, 1994 - v2
Checksum:i0435BDA0196B2D6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331 – 332LG → W in AAA35108 (PubMed:8516289).Curated2
Sequence conflicti341 – 343NSI → TLS in AAA35108 (PubMed:8516289).Curated3
Sequence conflicti821A → R in BAA02301 (PubMed:8455598).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12917 Genomic DNA. Translation: BAA02301.1.
L01620 Genomic DNA. Translation: AAA35108.1.
L23856 Genomic DNA. Translation: AAA35115.1.
L07865 Unassigned DNA. Translation: AAA16896.1.
Z47746 Genomic DNA. Translation: CAA87679.1.
BK006938 Genomic DNA. Translation: DAA11949.1.
PIRiS51254.
RefSeqiNP_010388.1. NM_001180411.1.

Genome annotation databases

EnsemblFungiiYDR103W; YDR103W; YDR103W.
GeneIDi851680.
KEGGisce:YDR103W.

Similar proteinsi

Entry informationi

Entry nameiSTE5_YEAST
AccessioniPrimary (citable) accession number: P32917
Secondary accession number(s): D6VS89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: September 27, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names