P32917 (STE5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein STE5

Gene names

Name:	STE5
Synonyms:	NUL3
Ordered Locus Names:	YDR103W
ORF Names:	YD8557.12

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone.
Subcellular location	Cytoplasm.
Post-translational modification	May be regulated at the phosphorylation level, and by the mating type of the cell and depends on an intact pheromone-response pathway.
Miscellaneous	Present with 1900 molecules/cell in log phase SD medium.
Sequence similarities	To yeast FAR1.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	invasive growth in response to glucose limitation Inferred from mutant phenotype PubMed 15192700. Source: SGD negative regulation of MAPK cascade Inferred from mutant phenotype PubMed 16424299. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from physical interaction PubMed 8062390. Source: SGD positive regulation of protein phosphorylation Inferred from direct assay PubMed 19303851. Source: SGD regulation of transposition, RNA-mediated Inferred from mutant phenotype PubMed 9566871. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11781566. Source: SGD mating projection tip Inferred from direct assay PubMed 11781566PubMed 17952059. Source: SGD nucleus Inferred from direct assay PubMed 9732267. Source: SGD plasma membrane Inferred from direct assay PubMed 9732267. Source: SGD
Molecular_function	phosphatidylinositol-4,5-bisphosphate binding Inferred from direct assay PubMed 16847350. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FUS3	P16892	7	EBI-18373,EBI-7193
STE11	P23561	7	EBI-18373,EBI-18259
STE4	P18851	2	EBI-18373,EBI-7390
STE7	P06784	8	EBI-18373,EBI-18389

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 917

917

Protein STE5

PRO_0000072266

Regions

Compositional bias

775 – 876

102

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

329

1

Phosphoserine Ref.8

Modified residue

898

1

Phosphoserine Ref.9

Experimental info

Sequence conflict

331 – 332

2

LG → W in AAA35108. Ref.2

Sequence conflict

341 – 343

3

NSI → TLS in AAA35108. Ref.2

Sequence conflict

821

1

A → R in BAA02301. Ref.1

Secondary structure

1

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917

Helix Strand Turn

Details...

Helix

45 – 48

4

Helix

49 – 51

3

Turn

52 – 54

3

Helix

55 – 58

4

Helix

59 – 63

5

Turn

64 – 66

3

Helix

593 – 604

12

Beta strand

608 – 616

9

Helix

618 – 620

3

Beta strand

622 – 624

3

Helix

625 – 640

16

Beta strand

645 – 650

6

Beta strand

653 – 660

8

Helix

661 – 664

4

Helix

668 – 672

5

Helix

673 – 676

4

Helix

685 – 692

8

Beta strand

701 – 707

7

Turn

713 – 715

3

Helix

717 – 719

3

Helix

723 – 725

3

Beta strand

735 – 741

7

Turn

748 – 750

3

Beta strand

755 – 758

4

Helix

760 – 770

11

Sequences

	Sequence		Length	Mass (Da)
	P32917 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 0435BDA0196B2D6F	FASTA	917	102,727
	10 20 30 40 50 60 MMETPTDNIV SPFHNFGSST QYSGTLSRTP NQIIELEKPS TLSPLSRGKK WTEKLARFQR 70 80 90 100 110 120 SSAKKKRFSP SPISSSTFSF SPKSRVTSSN SSGNEDGNLM NTPSTVSTDY LPQHPHRTSS 130 140 150 160 170 180 LPRPNSNLFH ASNSNLSRAN EPPRAENLSD NIPPKVAPFG YPIQRTSIKK SFLNASCTLC 190 200 210 220 230 240 DEPISNRRKG EKIIELACGH LSHQECLIIS FGTTSKADVR ALFPFCTKCK KDTNKAVQCI 250 260 270 280 290 300 PENDELKDIL ISDFLIHKIP DSELSITPQS RFPPYSPLLP PFGLSYTPVE RQTIYSQAPS 310 320 330 340 350 360 LNPNLILAAP PKERNQIPQK KSNYTFLHSP LGHRRIPSGA NSILADTSVA LSANDSISAV 370 380 390 400 410 420 SNSVRAKDDE TKTTLPLLRS YFIQILLNNF QEELQDWRID GDYGLLRLVD KLMISKDGQR 430 440 450 460 470 480 YIQCWCFLFE DAFVIAEVDN DVDVLEIRLK NLEVFTPIAN LRMTTLEASV LKCTLNKQHC 490 500 510 520 530 540 ADLSDLYIVQ NINSDESTTV QKWISGILNQ DFVFNEDNIT STLPILPIIK NFSKDVGNGR 550 560 570 580 590 600 HETSTFLGLI NPNKVVEVGN VHDNDTVIIR RGFTLNSGEC SRQSTVDSIQ SVLTTISSIL 610 620 630 640 650 660 SLKREKPDNL AIILQIDFTK LKEEDSLIVV YNSLKALTIK FARLQFCFVD RNNYVLDYGS 670 680 690 700 710 720 VLHKIDSLDS ISNLKSKSSS TQFSPIWLKN TLYPENIHEH LGIVAVSNSN MEAKKSILFQ 730 740 750 760 770 780 DYRCFTSFGR RRPNELKIKV GYLNVDYSDK IDELVEASSW TFVLETLCYS FGLSFDEHDD 790 800 810 820 830 840 DDEEDNDDST DNELDNSSGS LSDAESTTTI HIDSPFDNEN ATANMVNDRN LLTEGEHSNI 850 860 870 880 890 900 ENLETVASSV QPALIPNIRF SLHSEEEGTN ENENENDMPV LLLSDMDKGI DGITRRSSFS 910 SLIESGNNNC PLHMDYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Function of the ste signal transduction pathway for mating pheromones sustains MAT alpha 1 transcription in Saccharomyces cerevisiae." Mukai Y., Harashima S., Oshima Y. Mol. Cell. Biol. 13:2050-2060(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants." Perlman R., Yablonski D., Simchen G., Levitzki A. Proc. Natl. Acad. Sci. U.S.A. 90:5474-5478(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence and characterization of the STE5 gene required for signal transduction and mating in Saccharomyces cerevisiae." Mackay V.L., Mathewes S., Bell L., O'Hara P.J. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1." Leberer E., Dignard D., Harcus D., Hougan L., Whiteway M., Thomas D.Y. Mol. Gen. Genet. 241:241-254(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBY939.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-898, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D12917 Genomic DNA. Translation: BAA02301.1.
L01620 Genomic DNA. Translation: AAA35108.1.
L23856 Genomic DNA. Translation: AAA35115.1.
L07865 Unassigned DNA. Translation: AAA16896.1.
Z47746 Genomic DNA. Translation: CAA87679.1.
BK006938 Genomic DNA. Translation: DAA11949.1.

PIR

S51254.

RefSeq

NP_010388.1. NM_001180411.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KGN	NMR	-	A	44-67	[»]
2L4U	NMR	-	A	44-67	[»]
3FZE	X-ray	1.60	A	593-786	[»]
4F2H	X-ray	3.19	A	583-787	[»]

ProteinModelPortal

P32917.

SMR

P32917. Positions 584-774.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-858N.

IntAct

P32917. 10 interactions.

MINT

MINT-1174440.

STRING

4932.YDR103W.

Proteomic databases

PaxDb

P32917.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR103W; YDR103W; YDR103W.

GeneID

851680.

KEGG

sce:YDR103W.

Organism-specific databases

CYGD

YDR103w.

SGD

S000002510. STE5.

Phylogenomic databases

eggNOG

NOG44001.

HOGENOM

HOG000065959.

KO

K11239.

OMA

TLCDEPI.

OrthoDB

EOG4T1MWD.

Gene expression databases

Genevestigator

P32917.

GermOnline

YDR103W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR021651. Scaffold_Ste5_Fus5-binding.
IPR021106. Ste5_Fus3-bd.
IPR001841. Znf_RING.
[Graphical view]

Pfam

PF11610. Ste5. 1 hit.
PF12194. Ste5_C. 1 hit.
[Graphical view]

SMART

SM00184. RING. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32917.

NextBio

969315.

Entry information

Entry name

STE5_YEAST

Accession

Primary (citable) accession number: P32917
Secondary accession number(s): D6VS89

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program