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Protein

Signal recognition particle receptor subunit alpha homolog

Gene

SRP101

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. GTP hydrolysis may enhance the fidelity of and provide unidirectionality to the targeting reaction. It is important but not essential for cell growth. May be directly involved in mitochondrial protein import.1 Publication

Miscellaneous

Present with 2000 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi404 – 411GTPBy similarity8
Nucleotide bindingi510 – 514GTPBy similarity5
Nucleotide bindingi572 – 575GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: SGD
  • signal recognition particle binding Source: SGD

GO - Biological processi

  • protein targeting to ER Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane Source: SGD

Keywordsi

Molecular functionReceptor
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29855-MONOMER

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle receptor subunit alpha homolog
Short name:
SR-alpha
Alternative name(s):
Docking protein alpha
Short name:
DP-alpha
Gene namesi
Name:SRP101
Ordered Locus Names:YDR292C
ORF Names:D9819.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR292C
SGDiS000002700 SRP101

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012161 – 621Signal recognition particle receptor subunit alpha homologAdd BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32916
PaxDbiP32916
PRIDEiP32916

PTM databases

iPTMnetiP32916

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SRP102P360575EBI-18098,EBI-18091

Protein-protein interaction databases

BioGridi32344, 333 interactors
DIPiDIP-1672N
IntActiP32916, 6 interactors
MINTiP32916
STRINGi4932.YDR292C

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi13 – 19Combined sources7
Helixi27 – 39Combined sources13
Helixi44 – 46Combined sources3
Helixi51 – 53Combined sources3
Beta strandi58 – 62Combined sources5
Beta strandi74 – 80Combined sources7
Turni81 – 84Combined sources4
Beta strandi85 – 93Combined sources9
Helixi96 – 115Combined sources20
Helixi118 – 125Combined sources8
Beta strandi127 – 129Combined sources3
Turni140 – 144Combined sources5
Helixi145 – 152Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NRJX-ray1.70A1-158[»]
ProteinModelPortaliP32916
SMRiP32916
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32916

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 158SRXAdd BLAST158

Domaini

The SRX domain is sufficient for interaction with GTP-bound SR-beta.

Sequence similaritiesi

Belongs to the GTP-binding SRP family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074936
HOGENOMiHOG000201671
InParanoidiP32916
KOiK13431
OMAiQAIKYAK
OrthoDBiEOG092C2S7B

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011012 Longin-like_dom_sf
IPR027417 P-loop_NTPase
IPR007222 Sig_recog_particle_rcpt_asu_N
IPR013822 Signal_recog_particl_SRP54_hlx
IPR036225 SRP/SRP_N
IPR000897 SRP54_GTPase_dom
IPR015284 SRX_dom
PfamiView protein in Pfam
PF04086 SRP-alpha_N, 1 hit
PF00448 SRP54, 2 hits
PF02881 SRP54_N, 1 hit
PF09201 SRX, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47364 SSF47364, 1 hit
SSF52540 SSF52540, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

P32916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDQLAVFTP QGQVLYQYNC LGKKFSEIQI NSFISQLITS PVTRKESVAN
60 70 80 90 100
ANTDGFDFNL LTINSEHKNS PSFNALFYLN KQPELYFVVT FAEQTLELNQ
110 120 130 140 150
ETQQTLALVL KLWNSLHLSE SILKNRQGQN EKNKHNYVDI LQGIEDDLKK
160 170 180 190 200
FEQYFRIKYE ESIKQDHINP DNFTKNGSVP QSHNKNTKKK LRDTKGKKQS
210 220 230 240 250
TGNVGSGRKW GRDGGMLDEM NHEDAAKLDF SSSNSHNSSQ VALDSTINKD
260 270 280 290 300
SFGDRTEGGD FLIKEIDDLL SSHKDEITSG NEAKNSGYVS TAFGFLQKHV
310 320 330 340 350
LGNKTINESD LKSVLEKLTQ QLITKNVAPE AADYLTQQVS HDLVGSKTAN
360 370 380 390 400
WTSVENTARE SLTKALTQIL TPGVSVDLLR EIQSKRSKKD EEGKCDPYVF
410 420 430 440 450
SIVGVNGVGK STNLSKLAFW LLQNNFKVLI VACDTFRSGA VEQLRVHVEN
460 470 480 490 500
LAQLMDDSHV RGSKNKRGKT GNDYVELFEA GYGGSDLVTK IAKQAIKYSR
510 520 530 540 550
DQNFDIVLMD TAGRRHNDPT LMSPLKSFAD QAKPDKIIMV GEALVGTDSV
560 570 580 590 600
QQAKNFNDAF GKGRNLDFFI ISKCDTVGEM LGTMVNMVYA TGIPILFVGV
610 620
GQTYTDLRTL SVKWAVNTLM S
Length:621
Mass (Da):69,278
Last modified:November 1, 1997 - v2
Checksum:i032400E5B3310C91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti499S → A in AAA35093 (PubMed:1327299).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77274 Genomic DNA Translation: AAA35093.1
U51031 Genomic DNA Translation: AAB64468.1
BK006938 Genomic DNA Translation: DAA12131.1
PIRiS70121
RefSeqiNP_010578.3, NM_001180600.3

Genome annotation databases

EnsemblFungiiYDR292C; YDR292C; YDR292C
GeneIDi851886
KEGGisce:YDR292C

Similar proteinsi

Entry informationi

Entry nameiSRPR_YEAST
AccessioniPrimary (citable) accession number: P32916
Secondary accession number(s): D6VSS1, Q05553
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health