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Protein

Signal recognition particle receptor subunit alpha homolog

Gene

SRP101

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. GTP hydrolysis may enhance the fidelity of and provide unidirectionality to the targeting reaction. It is important but not essential for cell growth. May be directly involved in mitochondrial protein import.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi404 – 411GTPBy similarity8
Nucleotide bindingi510 – 514GTPBy similarity5
Nucleotide bindingi572 – 575GTPBy similarity4

GO - Molecular functioni

  • GTP binding Source: SGD
  • signal recognition particle binding Source: SGD

GO - Biological processi

  • protein targeting to ER Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29855-MONOMER.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle receptor subunit alpha homolog
Short name:
SR-alpha
Alternative name(s):
Docking protein alpha
Short name:
DP-alpha
Gene namesi
Name:SRP101
Ordered Locus Names:YDR292C
ORF Names:D9819.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR292C.
SGDiS000002700. SRP101.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Thought to be anchored in the membrane through an interaction with SR-beta, which contains a bona fide transmembrane domain.

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • signal recognition particle receptor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012161 – 621Signal recognition particle receptor subunit alpha homologAdd BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32916.
PRIDEiP32916.

PTM databases

iPTMnetiP32916.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SRP102P360575EBI-18098,EBI-18091

Protein-protein interaction databases

BioGridi32344. 132 interactors.
DIPiDIP-1672N.
IntActiP32916. 3 interactors.
MINTiMINT-364995.

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi13 – 19Combined sources7
Helixi27 – 39Combined sources13
Helixi44 – 46Combined sources3
Helixi51 – 53Combined sources3
Beta strandi58 – 62Combined sources5
Beta strandi74 – 80Combined sources7
Turni81 – 84Combined sources4
Beta strandi85 – 93Combined sources9
Helixi96 – 115Combined sources20
Helixi118 – 125Combined sources8
Beta strandi127 – 129Combined sources3
Turni140 – 144Combined sources5
Helixi145 – 152Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NRJX-ray1.70A1-158[»]
ProteinModelPortaliP32916.
SMRiP32916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32916.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 158SRXAdd BLAST158

Domaini

The SRX domain is sufficient for interaction with GTP-bound SR-beta.

Sequence similaritiesi

Belongs to the GTP-binding SRP family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074936.
HOGENOMiHOG000201671.
InParanoidiP32916.
KOiK13431.
OMAiGRRHNDP.
OrthoDBiEOG092C2S7B.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011012. Longin-like_dom.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR000897. SRP54_GTPase_dom.
IPR015284. SRX_dom.
[Graphical view]
PfamiPF00448. SRP54. 2 hits.
PF02881. SRP54_N. 1 hit.
PF09201. SRX. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDQLAVFTP QGQVLYQYNC LGKKFSEIQI NSFISQLITS PVTRKESVAN
60 70 80 90 100
ANTDGFDFNL LTINSEHKNS PSFNALFYLN KQPELYFVVT FAEQTLELNQ
110 120 130 140 150
ETQQTLALVL KLWNSLHLSE SILKNRQGQN EKNKHNYVDI LQGIEDDLKK
160 170 180 190 200
FEQYFRIKYE ESIKQDHINP DNFTKNGSVP QSHNKNTKKK LRDTKGKKQS
210 220 230 240 250
TGNVGSGRKW GRDGGMLDEM NHEDAAKLDF SSSNSHNSSQ VALDSTINKD
260 270 280 290 300
SFGDRTEGGD FLIKEIDDLL SSHKDEITSG NEAKNSGYVS TAFGFLQKHV
310 320 330 340 350
LGNKTINESD LKSVLEKLTQ QLITKNVAPE AADYLTQQVS HDLVGSKTAN
360 370 380 390 400
WTSVENTARE SLTKALTQIL TPGVSVDLLR EIQSKRSKKD EEGKCDPYVF
410 420 430 440 450
SIVGVNGVGK STNLSKLAFW LLQNNFKVLI VACDTFRSGA VEQLRVHVEN
460 470 480 490 500
LAQLMDDSHV RGSKNKRGKT GNDYVELFEA GYGGSDLVTK IAKQAIKYSR
510 520 530 540 550
DQNFDIVLMD TAGRRHNDPT LMSPLKSFAD QAKPDKIIMV GEALVGTDSV
560 570 580 590 600
QQAKNFNDAF GKGRNLDFFI ISKCDTVGEM LGTMVNMVYA TGIPILFVGV
610 620
GQTYTDLRTL SVKWAVNTLM S
Length:621
Mass (Da):69,278
Last modified:November 1, 1997 - v2
Checksum:i032400E5B3310C91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti499S → A in AAA35093 (PubMed:1327299).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77274 Genomic DNA. Translation: AAA35093.1.
U51031 Genomic DNA. Translation: AAB64468.1.
BK006938 Genomic DNA. Translation: DAA12131.1.
PIRiS70121.
RefSeqiNP_010578.3. NM_001180600.3.

Genome annotation databases

EnsemblFungiiYDR292C; YDR292C; YDR292C.
GeneIDi851886.
KEGGisce:YDR292C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77274 Genomic DNA. Translation: AAA35093.1.
U51031 Genomic DNA. Translation: AAB64468.1.
BK006938 Genomic DNA. Translation: DAA12131.1.
PIRiS70121.
RefSeqiNP_010578.3. NM_001180600.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NRJX-ray1.70A1-158[»]
ProteinModelPortaliP32916.
SMRiP32916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32344. 132 interactors.
DIPiDIP-1672N.
IntActiP32916. 3 interactors.
MINTiMINT-364995.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

PTM databases

iPTMnetiP32916.

Proteomic databases

MaxQBiP32916.
PRIDEiP32916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR292C; YDR292C; YDR292C.
GeneIDi851886.
KEGGisce:YDR292C.

Organism-specific databases

EuPathDBiFungiDB:YDR292C.
SGDiS000002700. SRP101.

Phylogenomic databases

GeneTreeiENSGT00550000074936.
HOGENOMiHOG000201671.
InParanoidiP32916.
KOiK13431.
OMAiGRRHNDP.
OrthoDBiEOG092C2S7B.

Enzyme and pathway databases

BioCyciYEAST:G3O-29855-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32916.
PROiP32916.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011012. Longin-like_dom.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR000897. SRP54_GTPase_dom.
IPR015284. SRX_dom.
[Graphical view]
PfamiPF00448. SRP54. 2 hits.
PF02881. SRP54_N. 1 hit.
PF09201. SRX. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRPR_YEAST
AccessioniPrimary (citable) accession number: P32916
Secondary accession number(s): D6VSS1, Q05553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.