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Protein

Protein transport protein SEC61

Gene

SEC61

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec61 complex, which is the major component of a channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2. In an initial step, the signal sequence seems to bind simultaneously to SEC61 and SEC62. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC61 mediates the association with the ribosome.

GO - Molecular functioni

  • peptide transporter activity Source: SGD
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: SGD
  • protein transporter activity Source: SGD
  • signal sequence binding Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • intracellular protein transmembrane import Source: SGD
  • misfolded protein transport Source: SGD
  • posttranslational protein targeting to membrane, translocation Source: SGD
  • retrograde protein transport, ER to cytosol Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32445-MONOMER.
ReactomeiR-SCE-381038. XBP1(S) activates chaperone genes.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC61
Alternative name(s):
Sec61 complex subunit SEC61
Sec61 complex subunit alpha
Gene namesi
Name:SEC61
Ordered Locus Names:YLR378C
ORF Names:L3502.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR378C.
SGDiS000004370. SEC61.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicCuratedAdd BLAST32
Transmembranei33 – 55Helical; Name=1CuratedAdd BLAST23
Topological domaini56 – 75LumenalCuratedAdd BLAST20
Transmembranei76 – 95Helical; Name=2CuratedAdd BLAST20
Topological domaini96 – 119CytoplasmicCuratedAdd BLAST24
Transmembranei120 – 141Helical; Name=3CuratedAdd BLAST22
Topological domaini142 – 146LumenalCurated5
Transmembranei147 – 167Helical; Name=4CuratedAdd BLAST21
Topological domaini168 – 212CytoplasmicCuratedAdd BLAST45
Transmembranei213 – 224Helical; Name=5CuratedAdd BLAST12
Topological domaini225 – 240LumenalCuratedAdd BLAST16
Transmembranei241 – 260Helical; Name=6CuratedAdd BLAST20
Topological domaini261 – 290CytoplasmicCuratedAdd BLAST30
Transmembranei291 – 311Helical; Name=7CuratedAdd BLAST21
Topological domaini312 – 361LumenalCuratedAdd BLAST50
Transmembranei362 – 381Helical; Name=8CuratedAdd BLAST20
Topological domaini382 – 416CytoplasmicCuratedAdd BLAST35
Transmembranei417 – 434Helical; Name=9CuratedAdd BLAST18
Topological domaini435 – 437LumenalCurated3
Transmembranei438 – 459Helical; Name=10CuratedAdd BLAST22
Topological domaini460 – 480CytoplasmicCuratedAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • Sec61 translocon complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273K → P or G: Severe growth defect. 1 Publication1
Mutagenesisi275R → D, G, P, Q or Y: Severe growth defect. 1 Publication1
Mutagenesisi275R → E, F or V: Severe growth defect; lowers SRP-dependent and SRP-independent translocation. 1 Publication1
Mutagenesisi276G → P: Severe growth defect. 1 Publication1
Mutagenesisi405K → D, E or P: Severe growth defect. 1 Publication1
Mutagenesisi406R → D: Severe growth defect; lowers SRP-dependent translocation. 1 Publication1
Mutagenesisi406R → E: Severe growth defect; lowers SRP-dependent and SRP-independent translocation. 1 Publication1
Mutagenesisi406R → H or W: Severe growth defect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001317891 – 480Protein transport protein SEC61Add BLAST480

Proteomic databases

MaxQBiP32915.
PRIDEiP32915.
TopDownProteomicsiP32915.

Interactioni

Subunit structurei

Component of the heterotrimeric Sec61 complex, which is composed of SEC61, SBH1 and SSS1. Presumably three to four Sec61 heterotrimers assemble into an oligomeric ring with a central aqueous pore. In cotranslational ER import, the pore diameter varies from 9-15 A in a ribosome-free resting state to 40-60 A in a functional state when associated with the ribosome. The Sec61 complex is part of a channel-forming translocon complex whose composition seem to change dependent upon different functional states. During post-translational ER import the Sec61 complex associates with the Sec62/63 complex to form the Sec complex. SEC61 interacts with STT3, OST1, OST2, OST4, SWP1 AND WBP1 components of the OT complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HRD3Q057872EBI-16400,EBI-31647
OST2P469642EBI-16400,EBI-12673
OST4Q993802EBI-16400,EBI-12689
PRC1P007294EBI-16400,EBI-4153
SEC62P2182510EBI-16400,EBI-16632
SEC66P337547EBI-16400,EBI-16647
SSS1P3517913EBI-16400,EBI-16406
STT3P390072EBI-16400,EBI-18447
SWP1Q027953EBI-16400,EBI-12666
WBP1P337672EBI-16400,EBI-12658

Protein-protein interaction databases

BioGridi31638. 182 interactors.
DIPiDIP-2433N.
IntActiP32915. 19 interactors.
MINTiMINT-547730.

Structurei

3D structure databases

ProteinModelPortaliP32915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003721.
HOGENOMiHOG000231249.
InParanoidiP32915.
KOiK10956.
OMAiWKAFAPT.
OrthoDBiEOG092C21L2.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
InterProiIPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNRVLDLF KPFESFLPEV IAPERKVPYN QKLIWTGVSL LIFLILGQIP
60 70 80 90 100
LYGIVSSETS DPLYWLRAML ASNRGTLLEL GVSPIITSSM IFQFLQGTQL
110 120 130 140 150
LQIRPESKQD RELFQIAQKV CAIILILGQA LVVVMTGNYG APSDLGLPIC
160 170 180 190 200
LLLIFQLMFA SLIVMLLDEL LSKGYGLGSG ISLFTATNIA EQIFWRAFAP
210 220 230 240 250
TTVNSGRGKE FEGAVIAFFH LLAVRKDKKR ALVEAFYRTN LPNMFQVLMT
260 270 280 290 300
VAIFLFVLYL QGFRYELPIR STKVRGQIGI YPIKLFYTSN TPIMLQSALT
310 320 330 340 350
SNIFLISQIL FQKYPTNPLI RLIGVWGIRP GTQGPQMALS GLAYYIQPLM
360 370 380 390 400
SLSEALLDPI KTIVYITFVL GSCAVFSKTW IEISGTSPRD IAKQFKDQGM
410 420 430 440 450
VINGKRETSI YRELKKIIPT AAAFGGATIG ALSVGSDLLG TLGSGASILM
460 470 480
ATTTIYGYYE AAAKEGGFTK NLVPGFSDLM
Length:480
Mass (Da):52,937
Last modified:October 1, 1993 - v1
Checksum:iAB34F12FB7B3CBD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62340 Genomic DNA. Translation: CAA44215.1.
U19104 Genomic DNA. Translation: AAB67276.1.
U19103 Genomic DNA. Translation: AAB67581.1.
BK006945 Genomic DNA. Translation: DAA09680.1.
PIRiA60043.
RefSeqiNP_013482.1. NM_001182267.1.

Genome annotation databases

EnsemblFungiiYLR378C; YLR378C; YLR378C.
GeneIDi851095.
KEGGisce:YLR378C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62340 Genomic DNA. Translation: CAA44215.1.
U19104 Genomic DNA. Translation: AAB67276.1.
U19103 Genomic DNA. Translation: AAB67581.1.
BK006945 Genomic DNA. Translation: DAA09680.1.
PIRiA60043.
RefSeqiNP_013482.1. NM_001182267.1.

3D structure databases

ProteinModelPortaliP32915.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31638. 182 interactors.
DIPiDIP-2433N.
IntActiP32915. 19 interactors.
MINTiMINT-547730.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

Proteomic databases

MaxQBiP32915.
PRIDEiP32915.
TopDownProteomicsiP32915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR378C; YLR378C; YLR378C.
GeneIDi851095.
KEGGisce:YLR378C.

Organism-specific databases

EuPathDBiFungiDB:YLR378C.
SGDiS000004370. SEC61.

Phylogenomic databases

GeneTreeiENSGT00390000003721.
HOGENOMiHOG000231249.
InParanoidiP32915.
KOiK10956.
OMAiWKAFAPT.
OrthoDBiEOG092C21L2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32445-MONOMER.
ReactomeiR-SCE-381038. XBP1(S) activates chaperone genes.

Miscellaneous databases

PROiP32915.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
InterProiIPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC61A_YEAST
AccessioniPrimary (citable) accession number: P32915
Secondary accession number(s): D6VZ14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 24800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.