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Protein

Transcription elongation factor SPT4

Gene

SPT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. The complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. Structural and functional component of the centromeric and heterochromatic loci linking chromatin structure with kinetochore function and gene silencing.7 Publications

Miscellaneous

Present with 4490 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in the transcription initiation step.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 27C4-typeSequence analysisAdd BLAST21

GO - Molecular functioni

  • DNA binding transcription factor activity Source: GO_Central
  • protein-containing complex binding Source: SGD
  • rDNA binding Source: SGD
  • RNA polymerase II core binding Source: SGD
  • transcription factor activity, core RNA polymerase I binding Source: SGD
  • transcription factor activity, core RNA polymerase II binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: SGD
  • chromatin organization Source: SGD
  • chromatin silencing Source: SGD
  • chromosome segregation Source: SGD
  • intracellular mRNA localization Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • negative regulation of transcription elongation by RNA polymerase I Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription elongation from RNA polymerase I promoter Source: SGD
  • regulation of rRNA processing Source: SGD
  • regulation of transcription, DNA-templated Source: SGD
  • regulation of transcription-coupled nucleotide-excision repair Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processmRNA processing, Transcription
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30778-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT4
Alternative name(s):
Chromatin elongation factor SPT4
Gene namesi
Name:SPT4
Ordered Locus Names:YGR063C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR063C
SGDiS000003295 SPT4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10C → Y: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103381 – 102Transcription elongation factor SPT4Add BLAST102

Proteomic databases

MaxQBiP32914
PaxDbiP32914
PRIDEiP32914

Interactioni

Subunit structurei

Component of the SPT4-SPT5 complex. Interacts with SHE2.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi33308, 241 interactors
DIPiDIP-2078N
IntActiP32914, 13 interactors
MINTiP32914
STRINGi4932.YGR063C

Structurei

Secondary structure

1102
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Turni8 – 10Combined sources3
Beta strandi12 – 15Combined sources4
Helixi16 – 22Combined sources7
Turni25 – 27Combined sources3
Helixi28 – 34Combined sources7
Helixi39 – 41Combined sources3
Beta strandi42 – 44Combined sources3
Beta strandi46 – 53Combined sources8
Turni55 – 57Combined sources3
Helixi59 – 63Combined sources5
Beta strandi71 – 78Combined sources8
Helixi84 – 87Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EXUX-ray2.23A1-99[»]
ProteinModelPortaliP32914
SMRiP32914
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32914

Family & Domainsi

Sequence similaritiesi

Belongs to the SPT4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 27C4-typeSequence analysisAdd BLAST21

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000018559
HOGENOMiHOG000172685
InParanoidiP32914
KOiK15171
OMAiVRGMYAV
OrthoDBiEOG092C5JQL

Family and domain databases

CDDicd07973 Spt4, 1 hit
Gene3Di3.30.40.210, 1 hit
InterProiView protein in InterPro
IPR029040 RPABC4/Spt4
IPR009287 Spt4
IPR022800 Spt4/RpoE2_Znf
IPR038510 Spt4_sf
PANTHERiPTHR12882 PTHR12882, 1 hit
PfamiView protein in Pfam
PF06093 Spt4, 1 hit
PIRSFiPIRSF025023 Spt4, 1 hit
SMARTiView protein in SMART
SM01389 Spt4, 1 hit
SUPFAMiSSF63393 SSF63393, 1 hit

Sequencei

Sequence statusi: Complete.

P32914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSERACMLC GIVQTTNEFN RDGCPNCQGI FEEAGVSTME CTSPSFEGLV
60 70 80 90 100
GMCKPTKSWV AKWLSVDHSI AGMYAIKVDG RLPAEVVELL PHYKPRDGSQ

VE
Length:102
Mass (Da):11,158
Last modified:October 1, 1993 - v1
Checksum:i59C164193ABA883B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83672 Genomic DNA Translation: AAA35084.1
DQ115391 Genomic DNA Translation: AAZ22476.1
AM296426 Genomic DNA Translation: CAL35887.1
AM296427 Genomic DNA Translation: CAL35886.1
AM296428 Genomic DNA Translation: CAL35885.1
AM296429 Genomic DNA Translation: CAL36074.1
AM296430 Genomic DNA Translation: CAL35884.1
AM296431 Genomic DNA Translation: CAL35883.1
AM296432 Genomic DNA Translation: CAL35882.1
AM296433 Genomic DNA Translation: CAL35881.1
AM296434 Genomic DNA Translation: CAL35880.1
AM296435 Genomic DNA Translation: CAL35879.1
AM296436 Genomic DNA Translation: CAL35878.1
AM296437 Genomic DNA Translation: CAL36075.1
AM296438 Genomic DNA Translation: CAL35877.1
Z72848 Genomic DNA Translation: CAA97065.1
AY693090 Genomic DNA Translation: AAT93109.1
BK006941 Genomic DNA Translation: DAA08159.1
PIRiS31176
RefSeqiNP_011577.3, NM_001181192.3

Genome annotation databases

EnsemblFungiiYGR063C; YGR063C; YGR063C
GeneIDi852955
KEGGisce:YGR063C

Similar proteinsi

Entry informationi

Entry nameiSPT4_YEAST
AccessioniPrimary (citable) accession number: P32914
Secondary accession number(s): D6VUJ8, Q0P6T7, Q45U23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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