Vacuolar protein sorting-associated protein 17 - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P32913 (VPS17_YEAST)

Last modified November 24, 2009. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 17
Alternative name(s):
Carboxypeptidase Y-deficient protein 21

Gene names

Name:	VPS17
Synonyms:	PEP21
Ordered Locus Names:	YOR132W
ORF Names:	O3314, YOR3314W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for the sorting and delivery of a subset of soluble vacuolar hydrolases. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS5 and VPS17.
Subcellular location	Endomembrane system; Peripheral membrane protein; Cytoplasmic side. Note: Membrane-associated on the cytoplasmic side of either the Golgi complex or an intermediate in Golgi to vacuole transport.
Post-translational modification	Phosphorylated on one or more serine residues. Ref.7 Ref.8
Miscellaneous	Present with 7380 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the VPS17 family. Contains 1 PX (phox homology) domain.

Hide | Top

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Ref.5 Inferred from physical interaction. Source: SGD
Cellular component	endosome Ref.5 Inferred from physical interaction. Source: SGD retromer complex, outer shell Ref.5 Inferred from physical interaction. Source: SGD
Molecular function	phosphatidylinositol 3-phosphate binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct
	P38800	1	EBI-20366,EBI-24597
ATG27	P46989	1	EBI-20366,EBI-26164
MVP1	P40959	1	EBI-20366,EBI-11636
PEP1	P32319	1	EBI-20366,EBI-13123
SNX4	P47057	1	EBI-20366,EBI-17610
VPS26	P40335	1	EBI-20366,EBI-20373
VPS29	P38759	1	EBI-20366,EBI-13092
VPS35	P34110	1	EBI-20366,EBI-20415
VPS5	Q92331	3	EBI-20366,EBI-20483
YIF1	P53845	1	EBI-20366,EBI-28230
YIP1	P53039	1	EBI-20366,EBI-25295
YIP4	P53093	1	EBI-20366,EBI-24124

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 551

551

Vacuolar protein sorting-associated protein 17

PRO_0000065892

Regions

Domain

108 – 227

120

Coiled coil

359 – 385

Potential

Amino acid modifications

Modified residue

417

Phosphoserine Ref.8

Modified residue

544

Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict

D → G in AAA35213. Ref.1

Sequence conflict

A → T in AAA35213. Ref.1

Sequence conflict

I → V in AAA35213. Ref.1

Sequence conflict

E → G in AAA35213. Ref.1

Sequence conflict

P → S in AAA35213. Ref.1

Sequence conflict

402

A → T in AAA35213. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P32913-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C709968468144A50
Show »

FASTA

551

63,204

        10         20         30         40         50         60 
MTSAVPYDPY DDLDNNPFAE PQEEDSEPAA TTTDGSSSMS EERVGTEQTA ASVQDNGTAN 

        70         80         90        100        110        120 
NIQNGLGEEG NATRSKTSNE HNENQQPSQP SERVILPERS DEKKKYTLLA KVTGLERFGS 

       130        140        150        160        170        180 
ATGKKENPTI IFDCSTNLPT FRKQQYKNVK KSYEEFHQLF KYLNVAIQES FVPTLPSAYT 

       190        200        210        220        230        240 
TFGINSEEDR MKVTRNFQLW FNRLSQDPLI IRNEEVAFFI ESDFNTYTPI NKSKSLASGL 

       250        260        270        280        290        300 
KRKTLKQLAP PYDEITELAE FRPLVKSIYV VSQSLQEKLL RVSRNRKMMV QEENAFGQDF 

       310        320        330        340        350        360 
VNLDEHNKLY RRYGKILTAV GDIDSIIATM DMATLYDGLE WIVRDAYAVK EALTNRHFIM 

       370        380        390        400        410        420 
RNLVQAQQNS KAKQEQARRF RSRRDINPMK IDEALRQLKA AAKNEQVLTL KLQRITSNMI 

       430        440        450        460        470        480 
IERKQWISWY EEWIRSSIKE FTLRKIEYER KKLTLLERVR SDIRKADENG GLSRLGRHAV 

       490        500        510        520        530        540 
SNNNSDTSQT LKGDSWTGES NRKSQIPINK IAHTEFDDEL FTEDDGYNSQ DSDTTSLNAR 

       550 
HAASLLGMST K

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast VPS17 gene encodes a membrane-associated protein required for the sorting of soluble vacuolar hydrolases." Koehrer K., Emr S.D. J. Biol. Chem. 268:559-569(1993) [PubMed: 8416961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417 AND SER-544, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	L02869 Genomic DNA. Translation: AAA35213.1. X94335 Genomic DNA. Translation: CAA64051.1. X90518 Genomic DNA. Translation: CAA62115.1. Z75040 Genomic DNA. Translation: CAA99331.1.
PIR	S60994.
RefSeq	NP_014775.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1739N.
IntAct	P32913. 19 interactions.
STRING	P32913.
Genome annotation databases
Ensembl	YOR132W; YOR132W; YOR132W; Saccharomyces cerevisiae. [Genome view]
GeneID	854300.
KEGG	sce:YOR132W.
NMPDR	fig\|4932.3.peg.5878.
Organism-specific databases
CYGD	YOR132w.
SGD	S000005658. VPS17.
Phylogenomic databases
HOGENOM	P32913.
OMA	EALTNRH
OrthoDB	EOG9GMWDF
Gene expression databases
ArrayExpress	P32913.
Genevestigator	P32913.
GermOnline	YOR132W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001683. PX. IPR014461. Vps17. [Graphical view]
Pfam	PF00787. PX. 1 hit. [Graphical view]
PIRSF	PIRSF011791. Vps17. 1 hit.
SMART	SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50195. PX. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976304.

Hide | Top

Entry information

Entry name

VPS17_YEAST

Accession

Primary (citable) accession number: P32913
Secondary accession number(s): Q12279

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents