Vacuolar morphogenesis protein 7 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P32912 (VAM7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar morphogenesis protein 7

Gene names

Name:	VAM7
Ordered Locus Names:	YGL212W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.
Subunit structure	Possibly multimeric. Associates with VAM3.
Subcellular location	Vacuole.
Miscellaneous	Present with 2360 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 PX (phox homology) domain. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro piecemeal microautophagy of nucleus Inferred from mutant phenotype PubMed 18701704. Source: SGD vacuole fusion, non-autophagic Inferred from direct assay PubMed 17400548. Source: SGD vesicle fusion Inferred from direct assay PubMed 11001046. Source: SGD
Cellular_component	SNARE complex Inferred from mutant phenotype PubMed 9628864. Source: SGD fungal-type vacuole membrane Inferred from direct assay PubMed 11433291. Source: SGD
Molecular_function	SNAP receptor activity Inferred from direct assay PubMed 19805279. Source: SGD phosphatidylinositol-3-phosphate binding Inferred from direct assay PubMed 11557775. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
RBD2	Q12270	3	EBI-20232,EBI-31471
VAM3	Q12241	4	EBI-20232,EBI-20227
YIF1	P53845	3	EBI-20232,EBI-28230

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 316

316

Vacuolar morphogenesis protein 7

PRO_0000065760

Regions

Domain

1 – 124

124

Domain

250 – 312

t-SNARE coiled-coil homology

Coiled coil

168 – 186

Potential

Secondary structure

316

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32912 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 2F992AEC0ACBC8FE
Show »

FASTA

316

36,711

        10         20         30         40         50         60 
MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT RLERDVGSTI 

        70         80         90        100        110        120 
PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY NDRFDSRWRD TKIAQDFLQL 

       130        140        150        160        170        180 
SKPNVSQEKS QQHLETADEV GWDEMIRDIK LDLDKESDGT PSVRGALRAR TKLHKLRERL 

       190        200        210        220        230        240 
EQDVQKKSLP STEVTRRAAL LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR 

       250        260        270        280        290        300 
TNNDLQQGQM QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR 

       310 
RLQIANKKAR HFNNSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles." Wada Y., Anraku Y. J. Biol. Chem. 267:18671-18675(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YWH019-7A.
[2]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames." Kail M., Juettner E., Vaux D. Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking." Sato T.K., Darsow T., Emr S.D. Mol. Cell. Biol. 18:5308-5319(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D11379 Genomic DNA. Translation: BAA01977.1.
U33754 Genomic DNA. Translation: AAC49494.1.
Z72734 Genomic DNA. Translation: CAA96928.1.
BK006941 Genomic DNA. Translation: DAA07904.1.

PIR

S31263.

RefSeq

NP_011303.1. NM_001181077.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KMD	NMR	-	A	8-124	[»]

ProteinModelPortal

P32912.

SMR

P32912. Positions 8-124.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1722N.

IntAct

P32912. 28 interactions.

MINT

MINT-384943.

STRING

4932.YGL212W.

Proteomic databases

PaxDb

P32912.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL212W; YGL212W; YGL212W.

GeneID

852660.

KEGG

sce:YGL212W.

Organism-specific databases

CYGD

YGL212w.

SGD

S000003180. VAM7.

Phylogenomic databases

eggNOG

NOG259218.

K08502.

OMA

PEMIDER.

OrthoDB

EOG4TQQJK.

Gene expression databases

Genevestigator

P32912.

GermOnline

YGL212W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.1520.10. 1 hit.

InterPro

IPR001683. Phox.
IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF00787. PX. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view]

SMART

SM00312. PX. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF64268. PX. 1 hit.

PROSITE

PS50195. PX. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32912.

NextBio

971942.

Entry information

Entry name

VAM7_YEAST

Accession

Primary (citable) accession number: P32912
Secondary accession number(s): D6VTU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents