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Protein

Vacuolar morphogenesis protein 7

Gene

VAM7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD
  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • autophagosome-lysosome fusion Source: SGD
  • macroautophagy Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30689-MONOMER.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar morphogenesis protein 7
Gene namesi
Name:VAM7
Ordered Locus Names:YGL212W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL212W.
SGDiS000003180. VAM7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657601 – 316Vacuolar morphogenesis protein 7Add BLAST316

Proteomic databases

MaxQBiP32912.
PRIDEiP32912.

PTM databases

iPTMnetiP32912.

Interactioni

Subunit structurei

Possibly multimeric. Associates with VAM3.

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi33044. 608 interactors.
DIPiDIP-1722N.
IntActiP32912. 36 interactors.
MINTiMINT-384943.
STRINGi4932.YGL212W.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 22Combined sources7
Beta strandi27 – 32Combined sources6
Beta strandi38 – 40Combined sources3
Helixi42 – 56Combined sources15
Helixi82 – 89Combined sources8
Helixi92 – 100Combined sources9
Helixi107 – 110Combined sources4
Helixi112 – 117Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMDNMR-A8-124[»]
ProteinModelPortaliP32912.
SMRiP32912.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32912.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124PXPROSITE-ProRule annotationAdd BLAST124
Domaini250 – 312t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 186Sequence analysisAdd BLAST19

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP32912.
KOiK08502.
OMAiQMWRRYS.
OrthoDBiEOG092C4Z3K.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiView protein in InterPro
IPR001683. Phox.
IPR036871. PX_dom_sf.
IPR000727. T_SNARE_dom.
PfamiView protein in Pfam
PF00787. PX. 1 hit.
SMARTiView protein in SMART
SM00312. PX. 1 hit.
SM00397. t_SNARE. 1 hit.
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiView protein in PROSITE
PS50195. PX. 1 hit.
PS50192. T_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

P32912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT
60 70 80 90 100
RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY
110 120 130 140 150
NDRFDSRWRD TKIAQDFLQL SKPNVSQEKS QQHLETADEV GWDEMIRDIK
160 170 180 190 200
LDLDKESDGT PSVRGALRAR TKLHKLRERL EQDVQKKSLP STEVTRRAAL
210 220 230 240 250
LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR TNNDLQQGQM
260 270 280 290 300
QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR
310
RLQIANKKAR HFNNSA
Length:316
Mass (Da):36,711
Last modified:October 1, 1993 - v1
Checksum:i2F992AEC0ACBC8FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11379 Genomic DNA. Translation: BAA01977.1.
U33754 Genomic DNA. Translation: AAC49494.1.
Z72734 Genomic DNA. Translation: CAA96928.1.
BK006941 Genomic DNA. Translation: DAA07904.1.
PIRiS31263.
RefSeqiNP_011303.1. NM_001181077.1.

Genome annotation databases

EnsemblFungiiYGL212W; YGL212W; YGL212W.
GeneIDi852660.
KEGGisce:YGL212W.

Similar proteinsi

Entry informationi

Entry nameiVAM7_YEAST
AccessioniPrimary (citable) accession number: P32912
Secondary accession number(s): D6VTU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 25, 2017
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names