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P32912

- VAM7_YEAST

UniProt

P32912 - VAM7_YEAST

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Protein

Vacuolar morphogenesis protein 7

Gene
VAM7, YGL212W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.

GO - Molecular functioni

  1. phosphatidylinositol-3-phosphate binding Source: SGD
  2. protein binding Source: IntAct
  3. SNAP receptor activity Source: SGD

GO - Biological processi

  1. piecemeal microautophagy of nucleus Source: SGD
  2. vacuole fusion, non-autophagic Source: SGD
  3. vesicle fusion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30689-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar morphogenesis protein 7
Gene namesi
Name:VAM7
Ordered Locus Names:YGL212W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL212w.
SGDiS000003180. VAM7.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Vacuolar morphogenesis protein 7PRO_0000065760Add
BLAST

Proteomic databases

MaxQBiP32912.
PaxDbiP32912.

Expressioni

Gene expression databases

GenevestigatoriP32912.

Interactioni

Subunit structurei

Possibly multimeric. Associates with VAM3.

Binary interactionsi

WithEntry#Exp.IntActNotes
RBD2Q122703EBI-20232,EBI-31471
VAM3Q122415EBI-20232,EBI-20227
YIF1P538453EBI-20232,EBI-28230

Protein-protein interaction databases

BioGridi33044. 258 interactions.
DIPiDIP-1722N.
IntActiP32912. 36 interactions.
MINTiMINT-384943.
STRINGi4932.YGL212W.

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 227
Beta strandi27 – 326
Beta strandi38 – 403
Helixi42 – 5615
Helixi82 – 898
Helixi92 – 1009
Helixi107 – 1104
Helixi112 – 1176

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMDNMR-A8-124[»]
ProteinModelPortaliP32912.
SMRiP32912. Positions 8-124.

Miscellaneous databases

EvolutionaryTraceiP32912.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 124124PXAdd
BLAST
Domaini250 – 31263t-SNARE coiled-coil homologyAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili168 – 18619 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG259218.
KOiK08502.
OMAiKLPLRSY.
OrthoDBiEOG7DRJF0.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00787. PX. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32912-1 [UniParc]FASTAAdd to Basket

« Hide

MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT    50
RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY 100
NDRFDSRWRD TKIAQDFLQL SKPNVSQEKS QQHLETADEV GWDEMIRDIK 150
LDLDKESDGT PSVRGALRAR TKLHKLRERL EQDVQKKSLP STEVTRRAAL 200
LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR TNNDLQQGQM 250
QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR 300
RLQIANKKAR HFNNSA 316
Length:316
Mass (Da):36,711
Last modified:October 1, 1993 - v1
Checksum:i2F992AEC0ACBC8FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11379 Genomic DNA. Translation: BAA01977.1.
U33754 Genomic DNA. Translation: AAC49494.1.
Z72734 Genomic DNA. Translation: CAA96928.1.
BK006941 Genomic DNA. Translation: DAA07904.1.
PIRiS31263.
RefSeqiNP_011303.1. NM_001181077.1.

Genome annotation databases

EnsemblFungiiYGL212W; YGL212W; YGL212W.
GeneIDi852660.
KEGGisce:YGL212W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11379 Genomic DNA. Translation: BAA01977.1 .
U33754 Genomic DNA. Translation: AAC49494.1 .
Z72734 Genomic DNA. Translation: CAA96928.1 .
BK006941 Genomic DNA. Translation: DAA07904.1 .
PIRi S31263.
RefSeqi NP_011303.1. NM_001181077.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KMD NMR - A 8-124 [» ]
ProteinModelPortali P32912.
SMRi P32912. Positions 8-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33044. 258 interactions.
DIPi DIP-1722N.
IntActi P32912. 36 interactions.
MINTi MINT-384943.
STRINGi 4932.YGL212W.

Proteomic databases

MaxQBi P32912.
PaxDbi P32912.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL212W ; YGL212W ; YGL212W .
GeneIDi 852660.
KEGGi sce:YGL212W.

Organism-specific databases

CYGDi YGL212w.
SGDi S000003180. VAM7.

Phylogenomic databases

eggNOGi NOG259218.
KOi K08502.
OMAi KLPLRSY.
OrthoDBi EOG7DRJF0.

Enzyme and pathway databases

BioCyci YEAST:G3O-30689-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32912.
NextBioi 971942.

Gene expression databases

Genevestigatori P32912.

Family and domain databases

Gene3Di 3.30.1520.10. 1 hit.
InterProi IPR001683. Phox.
IPR000727. T_SNARE_dom.
[Graphical view ]
Pfami PF00787. PX. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view ]
SMARTi SM00312. PX. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view ]
SUPFAMi SSF64268. SSF64268. 1 hit.
PROSITEi PS50195. PX. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles."
    Wada Y., Anraku Y.
    J. Biol. Chem. 267:18671-18675(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YWH019-7A.
  2. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
    Feuermann M., Simeonava L., Souciet J.-L., Potier S.
    Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
    Kail M., Juettner E., Vaux D.
    Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking."
    Sato T.K., Darsow T., Emr S.D.
    Mol. Cell. Biol. 18:5308-5319(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAM7_YEAST
AccessioniPrimary (citable) accession number: P32912
Secondary accession number(s): D6VTU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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