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P32912

- VAM7_YEAST

UniProt

P32912 - VAM7_YEAST

Protein

Vacuolar morphogenesis protein 7

Gene

VAM7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.

    GO - Molecular functioni

    1. phosphatidylinositol-3-phosphate binding Source: SGD
    2. protein binding Source: IntAct
    3. SNAP receptor activity Source: SGD

    GO - Biological processi

    1. piecemeal microautophagy of nucleus Source: SGD
    2. vacuole fusion, non-autophagic Source: SGD
    3. vesicle fusion Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30689-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar morphogenesis protein 7
    Gene namesi
    Name:VAM7
    Ordered Locus Names:YGL212W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL212w.
    SGDiS000003180. VAM7.

    Subcellular locationi

    GO - Cellular componenti

    1. fungal-type vacuole membrane Source: SGD
    2. SNARE complex Source: SGD

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Vacuolar morphogenesis protein 7PRO_0000065760Add
    BLAST

    Proteomic databases

    MaxQBiP32912.
    PaxDbiP32912.

    Expressioni

    Gene expression databases

    GenevestigatoriP32912.

    Interactioni

    Subunit structurei

    Possibly multimeric. Associates with VAM3.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RBD2Q122703EBI-20232,EBI-31471
    VAM3Q122415EBI-20232,EBI-20227
    YIF1P538453EBI-20232,EBI-28230

    Protein-protein interaction databases

    BioGridi33044. 258 interactions.
    DIPiDIP-1722N.
    IntActiP32912. 36 interactions.
    MINTiMINT-384943.
    STRINGi4932.YGL212W.

    Structurei

    Secondary structure

    1
    316
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 227
    Beta strandi27 – 326
    Beta strandi38 – 403
    Helixi42 – 5615
    Helixi82 – 898
    Helixi92 – 1009
    Helixi107 – 1104
    Helixi112 – 1176

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KMDNMR-A8-124[»]
    ProteinModelPortaliP32912.
    SMRiP32912. Positions 8-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32912.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 124124PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini250 – 31263t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili168 – 18619Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG259218.
    KOiK08502.
    OMAiKLPLRSY.
    OrthoDBiEOG7DRJF0.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF00787. PX. 1 hit.
    PF05739. SNARE. 1 hit.
    [Graphical view]
    SMARTiSM00312. PX. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF64268. SSF64268. 1 hit.
    PROSITEiPS50195. PX. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT    50
    RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY 100
    NDRFDSRWRD TKIAQDFLQL SKPNVSQEKS QQHLETADEV GWDEMIRDIK 150
    LDLDKESDGT PSVRGALRAR TKLHKLRERL EQDVQKKSLP STEVTRRAAL 200
    LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR TNNDLQQGQM 250
    QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR 300
    RLQIANKKAR HFNNSA 316
    Length:316
    Mass (Da):36,711
    Last modified:October 1, 1993 - v1
    Checksum:i2F992AEC0ACBC8FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11379 Genomic DNA. Translation: BAA01977.1.
    U33754 Genomic DNA. Translation: AAC49494.1.
    Z72734 Genomic DNA. Translation: CAA96928.1.
    BK006941 Genomic DNA. Translation: DAA07904.1.
    PIRiS31263.
    RefSeqiNP_011303.1. NM_001181077.1.

    Genome annotation databases

    EnsemblFungiiYGL212W; YGL212W; YGL212W.
    GeneIDi852660.
    KEGGisce:YGL212W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11379 Genomic DNA. Translation: BAA01977.1 .
    U33754 Genomic DNA. Translation: AAC49494.1 .
    Z72734 Genomic DNA. Translation: CAA96928.1 .
    BK006941 Genomic DNA. Translation: DAA07904.1 .
    PIRi S31263.
    RefSeqi NP_011303.1. NM_001181077.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KMD NMR - A 8-124 [» ]
    ProteinModelPortali P32912.
    SMRi P32912. Positions 8-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33044. 258 interactions.
    DIPi DIP-1722N.
    IntActi P32912. 36 interactions.
    MINTi MINT-384943.
    STRINGi 4932.YGL212W.

    Proteomic databases

    MaxQBi P32912.
    PaxDbi P32912.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL212W ; YGL212W ; YGL212W .
    GeneIDi 852660.
    KEGGi sce:YGL212W.

    Organism-specific databases

    CYGDi YGL212w.
    SGDi S000003180. VAM7.

    Phylogenomic databases

    eggNOGi NOG259218.
    KOi K08502.
    OMAi KLPLRSY.
    OrthoDBi EOG7DRJF0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30689-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32912.
    NextBioi 971942.

    Gene expression databases

    Genevestigatori P32912.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF00787. PX. 1 hit.
    PF05739. SNARE. 1 hit.
    [Graphical view ]
    SMARTi SM00312. PX. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64268. SSF64268. 1 hit.
    PROSITEi PS50195. PX. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles."
      Wada Y., Anraku Y.
      J. Biol. Chem. 267:18671-18675(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YWH019-7A.
    2. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
      Feuermann M., Simeonava L., Souciet J.-L., Potier S.
      Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
      Kail M., Juettner E., Vaux D.
      Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking."
      Sato T.K., Darsow T., Emr S.D.
      Mol. Cell. Biol. 18:5308-5319(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVAM7_YEAST
    AccessioniPrimary (citable) accession number: P32912
    Secondary accession number(s): D6VTU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2360 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3