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P32908

- SMC1_YEAST

UniProt

P32908 - SMC1_YEAST

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Protein
Structural maintenance of chromosomes protein 1
Gene
SMC1, CHL10, YFL008W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 408ATP Reviewed prediction

GO - Molecular functioni

  1. AT DNA binding Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. ATPase activity Source: SGD
  4. DNA secondary structure binding Source: SGD
  5. chromatin binding Source: InterPro
  6. double-stranded DNA binding Source: SGD
  7. identical protein binding Source: IntAct
  8. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. double-strand break repair Source: SGD
  3. mitotic sister chromatid cohesion Source: SGD
  4. mitotic sister chromatid segregation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30448-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Structural maintenance of chromosomes protein 1
Alternative name(s):
DA-box protein SMC1
Gene namesi
Name:SMC1
Synonyms:CHL10
Ordered Locus Names:YFL008W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL008w.
SGDiS000001886. SMC1.

Subcellular locationi

Nucleus. Chromosome
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

GO - Cellular componenti

  1. nuclear mitotic cohesin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1731S → L in temperature-sensitive mutant SMC1-2.
Mutagenesisi458 – 4581N → D in temperature-sensitive mutant SMC1-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12251225Structural maintenance of chromosomes protein 1
PRO_0000119011Add
BLAST

Proteomic databases

MaxQBiP32908.
PaxDbiP32908.
PeptideAtlasiP32908.

Expressioni

Gene expression databases

GenevestigatoriP32908.

Interactioni

Subunit structurei

Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1/SCC1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself8EBI-17402,EBI-17402
DYN1P360223EBI-17402,EBI-6230
IRR1P405412EBI-17402,EBI-16667
KAR3P171194EBI-17402,EBI-9499
MCD1Q121588EBI-17402,EBI-16655
NNF1P471496EBI-17402,EBI-12098
NNF2P532534EBI-17402,EBI-23229
PDS5Q042642EBI-17402,EBI-13077
SMC2P389893EBI-17402,EBI-17412
SMC3P4703720EBI-17402,EBI-17423
SMC3Q9UQE74EBI-17402,EBI-80718From a different organism.
SPC42P360943EBI-17402,EBI-17777

Protein-protein interaction databases

BioGridi31139. 159 interactions.
DIPiDIP-2982N.
IntActiP32908. 26 interactions.
MINTiMINT-434606.
STRINGi4932.YFL008W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118
Beta strandi17 – 226
Beta strandi27 – 326
Helixi39 – 4911
Helixi63 – 653
Beta strandi91 – 10010
Beta strandi103 – 11210
Beta strandi117 – 1215
Beta strandi124 – 1263
Helixi128 – 13710
Turni142 – 1443
Helixi156 – 1594
Helixi162 – 1676
Helixi1068 – 108114
Beta strandi1096 – 10994
Beta strandi1114 – 11174
Helixi1126 – 11283
Helixi1131 – 114616
Beta strandi1152 – 11587
Turni1159 – 11624
Helixi1165 – 117814
Beta strandi1183 – 11886
Helixi1192 – 11954
Beta strandi1199 – 12079
Turni1208 – 12114
Beta strandi1212 – 12198
Helixi1220 – 12223

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W1WX-ray2.90A/B/C/D1-214[»]
A/B/C/D1024-1225[»]
ProteinModelPortaliP32908.
SMRiP32908. Positions 2-205, 1048-1223.

Miscellaneous databases

EvolutionaryTraceiP32908.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni490 – 678189Flexible hinge
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili173 – 489317 Reviewed prediction
Add
BLAST
Coiled coili679 – 1063385 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1057 – 10615Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1129 – 116436Ala/Asp-rich (DA-box)
Add
BLAST

Domaini

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable MCD1 protein, forming a ring structure.

Sequence similaritiesi

Belongs to the SMC family. SMC1 subfamily.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG1196.
GeneTreeiENSGT00580000081569.
HOGENOMiHOG000195481.
KOiK06636.
OMAiFKSYRGH.
OrthoDBiEOG7M0P0T.

Family and domain databases

Gene3Di3.40.50.300. 5 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR028468. Smc1.
IPR010935. SMC_hinge.
[Graphical view]
PANTHERiPTHR18937:SF12. PTHR18937:SF12. 1 hit.
PfamiPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005719. SMC. 1 hit.
SMARTiSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.

Sequencei

Sequence statusi: Complete.

P32908-1 [UniParc]FASTAAdd to Basket

« Hide

MGRLVGLELS NFKSYRGVTK VGFGESNFTS IIGPNGSGKS NMMDAISFVL     50
GVRSNHLRSN ILKDLIYRGV LNDENSDDYD NEGAASSNPQ SAYVKAFYQK 100
GNKLVELMRI ISRNGDTSYK IDGKTVSYKD YSIFLENENI LIKAKNFLVF 150
QGDVEQIAAQ SPVELSRMFE EVSGSIQYKK EYEELKEKIE KLSKSATESI 200
KNRRRIHGEL KTYKEGINKN EEYRKQLDKK NELQKFQALW QLYHLEQQKE 250
ELTDKLSALN SEISSLKGKI NNEMKSLQRS KSSFVKESAV ISKQKSKLDY 300
IFKDKEKLVS DLRLIKVPQQ AAGKRISHIE KRIESLQKDL QRQKTYVERF 350
ETQLKVVTRS KEAFEEEIKQ SARNYDKFKL NENDLKTYNC LHEKYLTEGG 400
SILEEKIAVL NNDKREIQEE LERFNKRADI SKRRITEELS ITGEKLDTQL 450
NDLRVSLNEK NALHTERLHE LKKLQSDIES ANNQEYDLNF KLRETLVKID 500
DLSANQRETM KERKLRENIA MLKRFFPGVK GLVHDLCHPK KEKYGLAVST 550
ILGKNFDSVI VENLTVAQEC IAFLKKQRAG TASFIPLDTI ETELPTLSLP 600
DSQDYILSIN AIDYEPEYEK AMQYVCGDSI ICNTLNIAKD LKWKKGIRGK 650
LVTIEGALIH KAGLMTGGIS GDANNRWDKE EYQSLMSLKD KLLIQIDELS 700
NGQRSNSIRA REVENSVSLL NSDIANLRTQ VTQQKRSLDE NRLEIKYHND 750
LIEKEIQPKI TELKKKLDDL ENTKDNLVKE KEALQNNIFK EFTSKIGFTI 800
KEYENHSGEL MRQQSKELQQ LQKQILTVEN KLQFETDRLS TTQRRYEKAQ 850
KDLENAQVEM KSLEEQEYAI EMKIGSIESK LEEHKNHLDE LQKKFVTKQS 900
ELNSSEDILE DMNSNLQVLK RERDGIKEDI EKFDLERVTA LKNCKISNIN 950
IPISSETTID DLPISSTDNE AITISNSIDI NYKGLPKKYK ENNTDSARKE 1000
LEQKIHEVEE ILNELQPNAR ALERYDEAEG RFEVINNETE QLKAEEKKIL 1050
NQFLKIKKKR KELFEKTFDY VSDHLDAIYR ELTKNPNSNV ELAGGNASLT 1100
IEDEDEPFNA GIKYHATPPL KRFKDMEYLS GGEKTVAALA LLFAINSYQP 1150
SPFFVLDEVD AALDITNVQR IAAYIRRHRN PDLQFIVISL KNTMFEKSDA 1200
LVGVYRQQQE NSSKIITLDL SNYAE 1225
Length:1,225
Mass (Da):141,280
Last modified:October 1, 1993 - v1
Checksum:iB504017AA0ECCA8C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00602 Unassigned DNA. Translation: AAA16595.1.
D50617 Genomic DNA. Translation: BAA09230.1.
BK006940 Genomic DNA. Translation: DAA12432.1.
PIRiA49464.
RefSeqiNP_116647.1. NM_001179958.1.

Genome annotation databases

EnsemblFungiiYFL008W; YFL008W; YFL008W.
GeneIDi850540.
KEGGisce:YFL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00602 Unassigned DNA. Translation: AAA16595.1 .
D50617 Genomic DNA. Translation: BAA09230.1 .
BK006940 Genomic DNA. Translation: DAA12432.1 .
PIRi A49464.
RefSeqi NP_116647.1. NM_001179958.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W1W X-ray 2.90 A/B/C/D 1-214 [» ]
A/B/C/D 1024-1225 [» ]
ProteinModelPortali P32908.
SMRi P32908. Positions 2-205, 1048-1223.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31139. 159 interactions.
DIPi DIP-2982N.
IntActi P32908. 26 interactions.
MINTi MINT-434606.
STRINGi 4932.YFL008W.

Proteomic databases

MaxQBi P32908.
PaxDbi P32908.
PeptideAtlasi P32908.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL008W ; YFL008W ; YFL008W .
GeneIDi 850540.
KEGGi sce:YFL008W.

Organism-specific databases

CYGDi YFL008w.
SGDi S000001886. SMC1.

Phylogenomic databases

eggNOGi COG1196.
GeneTreei ENSGT00580000081569.
HOGENOMi HOG000195481.
KOi K06636.
OMAi FKSYRGH.
OrthoDBi EOG7M0P0T.

Enzyme and pathway databases

BioCyci YEAST:G3O-30448-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32908.
NextBioi 966301.
PROi P32908.

Gene expression databases

Genevestigatori P32908.

Family and domain databases

Gene3Di 3.40.50.300. 5 hits.
InterProi IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR028468. Smc1.
IPR010935. SMC_hinge.
[Graphical view ]
PANTHERi PTHR18937:SF12. PTHR18937:SF12. 1 hit.
Pfami PF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005719. SMC. 1 hit.
SMARTi SM00968. SMC_hinge. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family."
    Strunnikov A.V., Larionov V.L., Koshland D.
    J. Cell Biol. 123:1635-1648(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, MUTANTS SMC1-1 AND SMC1-2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
    Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
    Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; IRR1 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
  5. "Molecular architecture of SMC proteins and the yeast cohesin complex."
    Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
    Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; MCD1 AND IRR1, STRUCTURE.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSMC1_YEAST
AccessioniPrimary (citable) accession number: P32908
Secondary accession number(s): D6VTM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5710 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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