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P32908

- SMC1_YEAST

UniProt

P32908 - SMC1_YEAST

Protein

Structural maintenance of chromosomes protein 1

Gene

SMC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 408ATPSequence Analysis

    GO - Molecular functioni

    1. AT DNA binding Source: SGD
    2. ATPase activity Source: SGD
    3. ATP binding Source: UniProtKB-KW
    4. chromatin binding Source: InterPro
    5. DNA secondary structure binding Source: SGD
    6. double-stranded DNA binding Source: SGD
    7. identical protein binding Source: IntAct
    8. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. double-strand break repair Source: SGD
    3. mitotic sister chromatid cohesion Source: SGD
    4. mitotic sister chromatid segregation Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30448-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Structural maintenance of chromosomes protein 1
    Alternative name(s):
    DA-box protein SMC1
    Gene namesi
    Name:SMC1
    Synonyms:CHL10
    Ordered Locus Names:YFL008W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL008w.
    SGDiS000001886. SMC1.

    Subcellular locationi

    Nucleus. Chromosome
    Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

    GO - Cellular componenti

    1. nuclear mitotic cohesin complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi173 – 1731S → L in temperature-sensitive mutant SMC1-2.
    Mutagenesisi458 – 4581N → D in temperature-sensitive mutant SMC1-1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12251225Structural maintenance of chromosomes protein 1PRO_0000119011Add
    BLAST

    Proteomic databases

    MaxQBiP32908.
    PaxDbiP32908.
    PeptideAtlasiP32908.

    Expressioni

    Gene expression databases

    GenevestigatoriP32908.

    Interactioni

    Subunit structurei

    Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1/SCC1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself8EBI-17402,EBI-17402
    DYN1P360223EBI-17402,EBI-6230
    IRR1P405412EBI-17402,EBI-16667
    KAR3P171194EBI-17402,EBI-9499
    MCD1Q121588EBI-17402,EBI-16655
    NNF1P471496EBI-17402,EBI-12098
    NNF2P532534EBI-17402,EBI-23229
    PDS5Q042642EBI-17402,EBI-13077
    SMC2P389893EBI-17402,EBI-17412
    SMC3P4703720EBI-17402,EBI-17423
    SMC3Q9UQE74EBI-17402,EBI-80718From a different organism.
    SPC42P360943EBI-17402,EBI-17777

    Protein-protein interaction databases

    BioGridi31139. 159 interactions.
    DIPiDIP-2982N.
    IntActiP32908. 26 interactions.
    MINTiMINT-434606.
    STRINGi4932.YFL008W.

    Structurei

    Secondary structure

    1
    1225
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 118
    Beta strandi17 – 226
    Beta strandi27 – 326
    Helixi39 – 4911
    Helixi63 – 653
    Beta strandi91 – 10010
    Beta strandi103 – 11210
    Beta strandi117 – 1215
    Beta strandi124 – 1263
    Helixi128 – 13710
    Turni142 – 1443
    Helixi156 – 1594
    Helixi162 – 1676
    Helixi1068 – 108114
    Beta strandi1096 – 10994
    Beta strandi1114 – 11174
    Helixi1126 – 11283
    Helixi1131 – 114616
    Beta strandi1152 – 11587
    Turni1159 – 11624
    Helixi1165 – 117814
    Beta strandi1183 – 11886
    Helixi1192 – 11954
    Beta strandi1199 – 12079
    Turni1208 – 12114
    Beta strandi1212 – 12198
    Helixi1220 – 12223

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W1WX-ray2.90A/B/C/D1-214[»]
    A/B/C/D1024-1225[»]
    ProteinModelPortaliP32908.
    SMRiP32908. Positions 2-205, 1048-1223.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32908.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni490 – 678189Flexible hingeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili173 – 489317Sequence AnalysisAdd
    BLAST
    Coiled coili679 – 1063385Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1057 – 10615Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1129 – 116436Ala/Asp-rich (DA-box)Add
    BLAST

    Domaini

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable MCD1 protein, forming a ring structure.

    Sequence similaritiesi

    Belongs to the SMC family. SMC1 subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG1196.
    GeneTreeiENSGT00580000081569.
    HOGENOMiHOG000195481.
    KOiK06636.
    OMAiFKSYRGH.
    OrthoDBiEOG7M0P0T.

    Family and domain databases

    Gene3Di3.40.50.300. 5 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR024704. SMC.
    IPR028468. Smc1.
    IPR010935. SMC_hinge.
    [Graphical view]
    PANTHERiPTHR18937:SF12. PTHR18937:SF12. 1 hit.
    PfamiPF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005719. SMC. 1 hit.
    SMARTiSM00968. SMC_hinge. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32908-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRLVGLELS NFKSYRGVTK VGFGESNFTS IIGPNGSGKS NMMDAISFVL     50
    GVRSNHLRSN ILKDLIYRGV LNDENSDDYD NEGAASSNPQ SAYVKAFYQK 100
    GNKLVELMRI ISRNGDTSYK IDGKTVSYKD YSIFLENENI LIKAKNFLVF 150
    QGDVEQIAAQ SPVELSRMFE EVSGSIQYKK EYEELKEKIE KLSKSATESI 200
    KNRRRIHGEL KTYKEGINKN EEYRKQLDKK NELQKFQALW QLYHLEQQKE 250
    ELTDKLSALN SEISSLKGKI NNEMKSLQRS KSSFVKESAV ISKQKSKLDY 300
    IFKDKEKLVS DLRLIKVPQQ AAGKRISHIE KRIESLQKDL QRQKTYVERF 350
    ETQLKVVTRS KEAFEEEIKQ SARNYDKFKL NENDLKTYNC LHEKYLTEGG 400
    SILEEKIAVL NNDKREIQEE LERFNKRADI SKRRITEELS ITGEKLDTQL 450
    NDLRVSLNEK NALHTERLHE LKKLQSDIES ANNQEYDLNF KLRETLVKID 500
    DLSANQRETM KERKLRENIA MLKRFFPGVK GLVHDLCHPK KEKYGLAVST 550
    ILGKNFDSVI VENLTVAQEC IAFLKKQRAG TASFIPLDTI ETELPTLSLP 600
    DSQDYILSIN AIDYEPEYEK AMQYVCGDSI ICNTLNIAKD LKWKKGIRGK 650
    LVTIEGALIH KAGLMTGGIS GDANNRWDKE EYQSLMSLKD KLLIQIDELS 700
    NGQRSNSIRA REVENSVSLL NSDIANLRTQ VTQQKRSLDE NRLEIKYHND 750
    LIEKEIQPKI TELKKKLDDL ENTKDNLVKE KEALQNNIFK EFTSKIGFTI 800
    KEYENHSGEL MRQQSKELQQ LQKQILTVEN KLQFETDRLS TTQRRYEKAQ 850
    KDLENAQVEM KSLEEQEYAI EMKIGSIESK LEEHKNHLDE LQKKFVTKQS 900
    ELNSSEDILE DMNSNLQVLK RERDGIKEDI EKFDLERVTA LKNCKISNIN 950
    IPISSETTID DLPISSTDNE AITISNSIDI NYKGLPKKYK ENNTDSARKE 1000
    LEQKIHEVEE ILNELQPNAR ALERYDEAEG RFEVINNETE QLKAEEKKIL 1050
    NQFLKIKKKR KELFEKTFDY VSDHLDAIYR ELTKNPNSNV ELAGGNASLT 1100
    IEDEDEPFNA GIKYHATPPL KRFKDMEYLS GGEKTVAALA LLFAINSYQP 1150
    SPFFVLDEVD AALDITNVQR IAAYIRRHRN PDLQFIVISL KNTMFEKSDA 1200
    LVGVYRQQQE NSSKIITLDL SNYAE 1225
    Length:1,225
    Mass (Da):141,280
    Last modified:October 1, 1993 - v1
    Checksum:iB504017AA0ECCA8C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00602 Unassigned DNA. Translation: AAA16595.1.
    D50617 Genomic DNA. Translation: BAA09230.1.
    BK006940 Genomic DNA. Translation: DAA12432.1.
    PIRiA49464.
    RefSeqiNP_116647.1. NM_001179958.1.

    Genome annotation databases

    EnsemblFungiiYFL008W; YFL008W; YFL008W.
    GeneIDi850540.
    KEGGisce:YFL008W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00602 Unassigned DNA. Translation: AAA16595.1 .
    D50617 Genomic DNA. Translation: BAA09230.1 .
    BK006940 Genomic DNA. Translation: DAA12432.1 .
    PIRi A49464.
    RefSeqi NP_116647.1. NM_001179958.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W1W X-ray 2.90 A/B/C/D 1-214 [» ]
    A/B/C/D 1024-1225 [» ]
    ProteinModelPortali P32908.
    SMRi P32908. Positions 2-205, 1048-1223.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31139. 159 interactions.
    DIPi DIP-2982N.
    IntActi P32908. 26 interactions.
    MINTi MINT-434606.
    STRINGi 4932.YFL008W.

    Proteomic databases

    MaxQBi P32908.
    PaxDbi P32908.
    PeptideAtlasi P32908.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL008W ; YFL008W ; YFL008W .
    GeneIDi 850540.
    KEGGi sce:YFL008W.

    Organism-specific databases

    CYGDi YFL008w.
    SGDi S000001886. SMC1.

    Phylogenomic databases

    eggNOGi COG1196.
    GeneTreei ENSGT00580000081569.
    HOGENOMi HOG000195481.
    KOi K06636.
    OMAi FKSYRGH.
    OrthoDBi EOG7M0P0T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30448-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32908.
    NextBioi 966301.
    PROi P32908.

    Gene expression databases

    Genevestigatori P32908.

    Family and domain databases

    Gene3Di 3.40.50.300. 5 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR024704. SMC.
    IPR028468. Smc1.
    IPR010935. SMC_hinge.
    [Graphical view ]
    PANTHERi PTHR18937:SF12. PTHR18937:SF12. 1 hit.
    Pfami PF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005719. SMC. 1 hit.
    SMARTi SM00968. SMC_hinge. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family."
      Strunnikov A.V., Larionov V.L., Koshland D.
      J. Cell Biol. 123:1635-1648(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, MUTANTS SMC1-1 AND SMC1-2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
      Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
      Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; IRR1 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
    5. "Molecular architecture of SMC proteins and the yeast cohesin complex."
      Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
      Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; MCD1 AND IRR1, STRUCTURE.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSMC1_YEAST
    AccessioniPrimary (citable) accession number: P32908
    Secondary accession number(s): D6VTM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5710 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3