Structural maintenance of chromosomes protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32908 (SMC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Structural maintenance of chromosomes protein 1

Alternative name(s):
DA-box protein SMC1

Gene names

Name:	SMC1
Synonyms:	CHL10
Ordered Locus Names:	YFL008W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.
Subunit structure	Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1/SCC1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes. Ref.4
Subcellular location	Nucleus. Chromosome. Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
Domain	The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable MCD1 protein, forming a ring structure.
Miscellaneous	Present with 5710 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SMC family. SMC1 subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Chromosome Nucleus
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from electronic annotation. Source: InterPro double-strand break repair Inferred from mutant phenotype. Source: SGD mitotic sister chromatid cohesion Inferred from genetic interaction. Source: SGD
Cellular component	nuclear mitotic cohesin complex Inferred from direct assay Ref.4. Source: SGD
Molecular function	AT DNA binding Inferred from direct assay. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA secondary structure binding Inferred from direct assay. Source: SGD double-stranded DNA binding Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		8	EBI-17402,EBI-17402
DYN1	P36022	3	EBI-17402,EBI-6230
KAR3	P17119	4	EBI-17402,EBI-9499
MCD1	Q12158	7	EBI-17402,EBI-16655
NNF1	P47149	6	EBI-17402,EBI-12098
NNF2	P53253	4	EBI-17402,EBI-23229
SMC2	P38989	3	EBI-17402,EBI-17412
SMC3	P47037	13	EBI-17402,EBI-17423
SMC3	Q9UQE7	4	EBI-17402,EBI-80718	From a different organism.
SPC42	P36094	3	EBI-17402,EBI-17777

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1225

1225

Structural maintenance of chromosomes protein 1

PRO_0000119011

Regions

Nucleotide binding

33 – 40

ATP Potential

Region

490 – 678

189

Flexible hinge

Coiled coil

173 – 489

317

Potential

Coiled coil

679 – 1063

385

Potential

Motif

1057 – 1061

Nuclear localization signal Potential

Compositional bias

1129 – 1164

Ala/Asp-rich (DA-box)

Amino acid modifications

Modified residue

327

Phosphoserine Ref.7

Experimental info

Mutagenesis

173

S → L in temperature-sensitive mutant SMC1-2.

Mutagenesis

458

N → D in temperature-sensitive mutant SMC1-1.

Secondary structure

1225

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32908 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: B504017AA0ECCA8C
Show »

FASTA

1,225

141,280

        10         20         30         40         50         60 
MGRLVGLELS NFKSYRGVTK VGFGESNFTS IIGPNGSGKS NMMDAISFVL GVRSNHLRSN 

        70         80         90        100        110        120 
ILKDLIYRGV LNDENSDDYD NEGAASSNPQ SAYVKAFYQK GNKLVELMRI ISRNGDTSYK 

       130        140        150        160        170        180 
IDGKTVSYKD YSIFLENENI LIKAKNFLVF QGDVEQIAAQ SPVELSRMFE EVSGSIQYKK 

       190        200        210        220        230        240 
EYEELKEKIE KLSKSATESI KNRRRIHGEL KTYKEGINKN EEYRKQLDKK NELQKFQALW 

       250        260        270        280        290        300 
QLYHLEQQKE ELTDKLSALN SEISSLKGKI NNEMKSLQRS KSSFVKESAV ISKQKSKLDY 

       310        320        330        340        350        360 
IFKDKEKLVS DLRLIKVPQQ AAGKRISHIE KRIESLQKDL QRQKTYVERF ETQLKVVTRS 

       370        380        390        400        410        420 
KEAFEEEIKQ SARNYDKFKL NENDLKTYNC LHEKYLTEGG SILEEKIAVL NNDKREIQEE 

       430        440        450        460        470        480 
LERFNKRADI SKRRITEELS ITGEKLDTQL NDLRVSLNEK NALHTERLHE LKKLQSDIES 

       490        500        510        520        530        540 
ANNQEYDLNF KLRETLVKID DLSANQRETM KERKLRENIA MLKRFFPGVK GLVHDLCHPK 

       550        560        570        580        590        600 
KEKYGLAVST ILGKNFDSVI VENLTVAQEC IAFLKKQRAG TASFIPLDTI ETELPTLSLP 

       610        620        630        640        650        660 
DSQDYILSIN AIDYEPEYEK AMQYVCGDSI ICNTLNIAKD LKWKKGIRGK LVTIEGALIH 

       670        680        690        700        710        720 
KAGLMTGGIS GDANNRWDKE EYQSLMSLKD KLLIQIDELS NGQRSNSIRA REVENSVSLL 

       730        740        750        760        770        780 
NSDIANLRTQ VTQQKRSLDE NRLEIKYHND LIEKEIQPKI TELKKKLDDL ENTKDNLVKE 

       790        800        810        820        830        840 
KEALQNNIFK EFTSKIGFTI KEYENHSGEL MRQQSKELQQ LQKQILTVEN KLQFETDRLS 

       850        860        870        880        890        900 
TTQRRYEKAQ KDLENAQVEM KSLEEQEYAI EMKIGSIESK LEEHKNHLDE LQKKFVTKQS 

       910        920        930        940        950        960 
ELNSSEDILE DMNSNLQVLK RERDGIKEDI EKFDLERVTA LKNCKISNIN IPISSETTID 

       970        980        990       1000       1010       1020 
DLPISSTDNE AITISNSIDI NYKGLPKKYK ENNTDSARKE LEQKIHEVEE ILNELQPNAR 

      1030       1040       1050       1060       1070       1080 
ALERYDEAEG RFEVINNETE QLKAEEKKIL NQFLKIKKKR KELFEKTFDY VSDHLDAIYR 

      1090       1100       1110       1120       1130       1140 
ELTKNPNSNV ELAGGNASLT IEDEDEPFNA GIKYHATPPL KRFKDMEYLS GGEKTVAALA 

      1150       1160       1170       1180       1190       1200 
LLFAINSYQP SPFFVLDEVD AALDITNVQR IAAYIRRHRN PDLQFIVISL KNTMFEKSDA 

      1210       1220 
LVGVYRQQQE NSSKIITLDL SNYAE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family." Strunnikov A.V., Larionov V.L., Koshland D. J. Cell Biol. 123:1635-1648(1993) [PubMed: 8276886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, MUTANTS SMC1-1 AND SMC1-2.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication." Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K. Genes Dev. 13:320-333(1999) [PubMed: 9990856] [Abstract] Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; IRR1 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
[5]	"Molecular architecture of SMC proteins and the yeast cohesin complex." Haering C.H., Loewe J., Hochwagen A., Nasmyth K. Mol. Cell 9:773-788(2002) [PubMed: 11983169] [Abstract] Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; MCD1 AND IRR1, STRUCTURE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L00602 Unassigned DNA. Translation: AAA16595.1.
D50617 Genomic DNA. Translation: BAA09230.1.
BK006940 Genomic DNA. Translation: DAA12432.1.

PIR

A49464.

RefSeq

NP_116647.1. NM_001179958.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W1W	X-ray	2.90	A/B/C/D	1-1225	[»]

ProteinModelPortal

P32908.

SMR

P32908. Positions 2-205, 517-674, 1048-1223.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2982N.

IntAct

P32908. 27 interactions.

MINT

MINT-434606.

STRING

P32908.

Proteomic databases

PeptideAtlas

P32908.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL008W; YFL008W; YFL008W.

GeneID

850540.

KEGG

sce:YFL008W.

NMPDR

fig|4932.3.peg.2278.

Organism-specific databases

CYGD

YFL008w.

SGD

S000001886. SMC1.

Phylogenomic databases

eggNOG

fuNOG06246.

GeneTree

EFGT00050000000440.

HOGENOM

HBG559513.

OMA

YQPSPFF.

OrthoDB

EOG42VCQD.

Gene expression databases

ArrayExpress

P32908.

Genevestigator

P32908.

GermOnline

YFL008W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003395. RecF/RecN/SMC.
IPR024704. SMC.
IPR010935. SMC_hinge.
[Graphical view]

K06636.

Pfam

PF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]

PIRSF

PIRSF005719. SMC. 1 hit.

SMART

SM00968. SMC_hinge. 1 hit.
[Graphical view]

SUPFAM

SSF75553. SMC_hinge. 1 hit.

ProtoNet

Search...

Other

NextBio

966301.

Entry information

Entry name

SMC1_YEAST

Accession

Primary (citable) accession number: P32908
Secondary accession number(s): D6VTM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents