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Reviewed, UniProtKB/Swiss-Prot P32906 (MNS1_YEAST)

Last modified June 16, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
    EC=3.2.1.113
Alternative name(s):
    ER alpha-1,2-mannosidase
    Man(9)-alpha-mannosidase
Gene names
Name: MNS1
Ordered Locus Names: YJR131W
ORF Names: J2110
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Trim a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2. The only product is the Man8GlcNAc2 isomer B, the form lacking the middle-arm terminal alpha 1,2-mannose. It may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Calcium.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Miscellaneous

Present with 8260 molecules/cell in log phase SD medium. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

YBR296C-AQ8TGU51EBI-11094,EBI-2042814

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
PRO_0000210319

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2420Signal-anchor for type II membrane protein Potential
Topological domain25 – 354330Lumenal Potential

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) Probable
Glycosylation1551N-linked (GlcNAc...) Probable
Glycosylation2241N-linked (GlcNAc...) Probable
Disulfide bond340 ↔ 385
Disulfide bond468 ↔ 471

Natural variations

Natural variant401D → Y
Natural variant3761D → L

Secondary structure

1 .................................................................................. 549
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P32906-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: B85FF351900A9BB2

FASTA54963,057
        10         20         30         40         50         60 
MKNSVGISIA TIVAIIAAIY YVPWYEHFER KSPGAGEMRD RIESMFLESW RDYSKHGWGY 

        70         80         90        100        110        120 
DVYGPIEHTS HNMPRGNQPL GWIIVDSVDT LMLMYNSSTL YKSEFEAEIQ RSEHWINDVL 

       130        140        150        160        170        180 
DFDIDAEVNV FETTIRMLGG LLSAYHLSDV LEVGNKTVYL NKAIDLGDRL ALAFLSTQTG 

       190        200        210        220        230        240 
IPYSSINLHS GQAVKNHADG GASSTAEFTT LQMEFKYLAY LTGNRTYWEL VERVYEPLYK 

       250        260        270        280        290        300 
NNDLLNTYDG LVPIYTFPDT GKFGASTIRF GSRGDSFYEY LLKQYLLTHE TLYYDLYRKS 

       310        320        330        340        350        360 
MEGMKKHLLA QSKPSSLWYI GEREQGLHGQ LSPKMDHLVC FMGGLLASGS TEGLSIHEAR 

       370        380        390        400        410        420 
RRPFFSLSLE RKSDWDLAKG ITDTCYQMYK QSSSGLAPEI VVFNDGNIKQ DGWWRSSVGD 

       430        440        450        460        470        480 
FFVKPLDRHN LQRPETVESI MFMYHLSHDH KYREWGAEIA TSFFENTCVD CNDPKLRRFT 

       490        500        510        520        530        540 
SLSDCITLPT KKSNNMESFW LAETLKYLYI LFLDEFDLTK VVFNTEAHPF PVLDEEILKS 


QSLTTGWSL

« Hide

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References

« Hide 'large scale' references
[1]"Glycoprotein biosynthesis in Saccharomyces cerevisiae. Isolation and characterization of the gene encoding a specific processing alpha-mannosidase."
Camirand A., Heysen A., Grondin B., Herscovics A.
J. Biol. Chem. 266:15120-15127(1991) [PubMed: 1714453] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Crystal structure of a class I alpha1,2-mannosidase involved in N-glycan processing and endoplasmic reticulum quality control."
Vallee F., Lipari F., Yip P., Sleno B., Herscovics A., Howell P.L.
EMBO J. 19:581-588(2000) [PubMed: 10675327] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 34-549.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M63598 Genomic DNA. Translation: AAA34799.1.
Z49631 Genomic DNA. Translation: CAA89662.1.
PIRA39345.
RefSeqNP_012665.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1DL2X-ray1.54A34-549[»]
1G6IX-ray1.59A1-549[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1441N.
IntActP32906. 10 interactions.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

Genome annotation databases

EnsemblYJR131W. Saccharomyces cerevisiae. [Contig view]
GeneID853595.
GenomeReviewsGene locus YJR131W in contig Y13136_GR.
KEGGsce:YJR131W.
NMPDRfig|4932.3.peg.3640.

Organism-specific databases

CYGDYJR131w.
SGDS000003892. MNS1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP32906.
OMAP32906. HRDFISV.

Enzyme and pathway databases

BRENDA3.2.1.113. 250.

Gene expression databases

ArrayExpressP32906.
GermOnlineYJR131W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001382. Glyco_hydro_47.
[Graphical view]
Gene3DG3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
PANTHERPTHR11742. Glyco_hydro_47. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
ProtoNetSearch...

Other Resources

NextBio974409.

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Entry information

Entry nameMNS1_YEAST
AccessionPrimary (citable) accession number: P32906
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents