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P32905

- RSSA1_YEAST

UniProt

P32905 - RSSA1_YEAST

Protein

40S ribosomal protein S0-A

Gene

RPS0A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.2 PublicationsUniRule annotation

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. ribosomal small subunit assembly Source: SGD
    5. rRNA export from nucleus Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30896-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S0-AUniRule annotation
    Alternative name(s):
    Nucleic acid-binding protein NAB1A
    Gene namesi
    Name:RPS0AUniRule annotation
    Synonyms:NAB1, NAB1A, YST1
    Ordered Locus Names:YGR214W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000003446. RPS0A.

    Subcellular locationi

    Cytoplasm 2 PublicationsUniRule annotation

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 PublicationsUniRule annotation
    Chaini2 – 25225140S ribosomal protein S0-APRO_0000134370Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 PublicationsUniRule annotation

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP32905.
    PeptideAtlasiP32905.

    2D gel databases

    SWISS-2DPAGEP32905.

    Expressioni

    Gene expression databases

    GenevestigatoriP32905.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

    Protein-protein interaction databases

    BioGridi33467. 100 interactions.
    DIPiDIP-5553N.
    IntActiP32905. 155 interactions.
    MINTiMINT-562708.
    STRINGi4932.YGR214W.

    Structurei

    Secondary structure

    1
    252
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 2010
    Turni21 – 233
    Helixi31 – 366
    Beta strandi37 – 415
    Turni42 – 443
    Beta strandi45 – 484
    Helixi50 – 6415
    Beta strandi67 – 693
    Helixi70 – 723
    Beta strandi73 – 775
    Helixi80 – 9213
    Beta strandi96 – 1005
    Beta strandi106 – 1083
    Beta strandi119 – 1246
    Turni126 – 1294
    Helixi130 – 1378
    Turni138 – 1403
    Beta strandi143 – 1475
    Beta strandi157 – 1615
    Helixi169 – 18214
    Beta strandi183 – 1875
    Beta strandi190 – 1923
    Helixi199 – 2013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Xmodel-B14-198[»]
    1S1Helectron microscopy11.70B14-198[»]
    1VW9electron microscopy6.10B1-252[»]
    1VWVelectron microscopy6.10B1-252[»]
    3IZBelectron microscopy-A1-252[»]
    3O2ZX-ray4.00A1-252[»]
    3O30X-ray4.00A1-252[»]
    3U5CX-ray3.00A1-252[»]
    3U5GX-ray3.00A1-252[»]
    4BYLelectron microscopy4.30A1-252[»]
    4BYTelectron microscopy6.60A1-252[»]
    4CUYelectron microscopy3.70A2-207[»]
    ProteinModelPortaliP32905.
    SMRiP32905. Positions 2-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32905.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S2P family.UniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00390000015036.
    HOGENOMiHOG000232073.
    KOiK02998.
    OMAiCHYQMER.
    OrthoDBiEOG741ZD0.

    Family and domain databases

    HAMAPiMF_03015. Ribosomal_S2_euk.
    InterProiIPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view]
    PANTHERiPTHR11489. PTHR11489. 1 hit.
    PfamiPF00318. Ribosomal_S2. 2 hits.
    [Graphical view]
    PRINTSiPR00395. RIBOSOMALS2.
    SUPFAMiSSF52313. SSF52313. 1 hit.
    TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32905-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV    50
    GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKFA AHTGATPIAG 100
    RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD 150
    SPSEFVDVAI PCNNRGKHSI GLIWYLLARE VLRLRGALVD RTQPWSIMPD 200
    LYFYRDPEEV EQQVAEEATT EEAGEEEAKE EVTEEQAEAT EWAEENADNV 250
    EW 252
    Length:252
    Mass (Da):28,024
    Last modified:January 23, 2007 - v3
    Checksum:iD6F263456282C771
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88277 Genomic DNA. Translation: AAB05643.1.
    Z72999 Genomic DNA. Translation: CAA97241.1.
    BK006941 Genomic DNA. Translation: DAA08308.1.
    PIRiS42143.
    RefSeqiNP_011730.1. NM_001181343.1.

    Genome annotation databases

    EnsemblFungiiYGR214W; YGR214W; YGR214W.
    GeneIDi853128.
    KEGGisce:YGR214W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88277 Genomic DNA. Translation: AAB05643.1 .
    Z72999 Genomic DNA. Translation: CAA97241.1 .
    BK006941 Genomic DNA. Translation: DAA08308.1 .
    PIRi S42143.
    RefSeqi NP_011730.1. NM_001181343.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5X model - B 14-198 [» ]
    1S1H electron microscopy 11.70 B 14-198 [» ]
    1VW9 electron microscopy 6.10 B 1-252 [» ]
    1VWV electron microscopy 6.10 B 1-252 [» ]
    3IZB electron microscopy - A 1-252 [» ]
    3O2Z X-ray 4.00 A 1-252 [» ]
    3O30 X-ray 4.00 A 1-252 [» ]
    3U5C X-ray 3.00 A 1-252 [» ]
    3U5G X-ray 3.00 A 1-252 [» ]
    4BYL electron microscopy 4.30 A 1-252 [» ]
    4BYT electron microscopy 6.60 A 1-252 [» ]
    4CUY electron microscopy 3.70 A 2-207 [» ]
    ProteinModelPortali P32905.
    SMRi P32905. Positions 2-207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33467. 100 interactions.
    DIPi DIP-5553N.
    IntActi P32905. 155 interactions.
    MINTi MINT-562708.
    STRINGi 4932.YGR214W.

    2D gel databases

    SWISS-2DPAGE P32905.

    Proteomic databases

    MaxQBi P32905.
    PeptideAtlasi P32905.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR214W ; YGR214W ; YGR214W .
    GeneIDi 853128.
    KEGGi sce:YGR214W.

    Organism-specific databases

    SGDi S000003446. RPS0A.

    Phylogenomic databases

    GeneTreei ENSGT00390000015036.
    HOGENOMi HOG000232073.
    KOi K02998.
    OMAi CHYQMER.
    OrthoDBi EOG741ZD0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30896-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P32905.
    NextBioi 973178.

    Gene expression databases

    Genevestigatori P32905.

    Family and domain databases

    HAMAPi MF_03015. Ribosomal_S2_euk.
    InterProi IPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view ]
    PANTHERi PTHR11489. PTHR11489. 1 hit.
    Pfami PF00318. Ribosomal_S2. 2 hits.
    [Graphical view ]
    PRINTSi PR00395. RIBOSOMALS2.
    SUPFAMi SSF52313. SSF52313. 1 hit.
    TIGRFAMsi TIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEi PS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
      Demianova M.A., Formosa T.G., Ellis S.R.
      J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: PROTEIN SEQUENCE OF 118-133 AND 231-248.
      Strain: ATCC 204508 / S288c.
    6. "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
      Norbeck J., Blomberg A.
      J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-36 AND 135-146.
      Strain: ATCC 44827 / SKQ2N.
    7. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
      Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
      Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT SER-2.
    8. "The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
      Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
      Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE EVOLUTION.
    9. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    10. "Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
      Ford C.L., Randal-Whitis L., Ellis S.R.
      Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    11. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
      Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
      Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    15. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
    16. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiRSSA1_YEAST
    AccessioniPrimary (citable) accession number: P32905
    Secondary accession number(s): D6VUZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 93700 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S0 in yeast.
    This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3