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P32905

- RSSA1_YEAST

UniProt

P32905 - RSSA1_YEAST

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Protein

40S ribosomal protein S0-A

Gene

RPS0A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.2 PublicationsUniRule annotation

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  3. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. ribosomal small subunit assembly Source: SGD
  5. rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30896-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S0-AUniRule annotation
Alternative name(s):
Nucleic acid-binding protein NAB1A
Gene namesi
Name:RPS0AUniRule annotation
Synonyms:NAB1, NAB1A, YST1
Ordered Locus Names:YGR214W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003446. RPS0A.

Subcellular locationi

Cytoplasm 2 PublicationsUniRule annotation

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 PublicationsUniRule annotation
Chaini2 – 25225140S ribosomal protein S0-APRO_0000134370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 PublicationsUniRule annotation

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP32905.
PeptideAtlasiP32905.

2D gel databases

SWISS-2DPAGEP32905.

Expressioni

Gene expression databases

GenevestigatoriP32905.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

Protein-protein interaction databases

BioGridi33467. 102 interactions.
DIPiDIP-5553N.
IntActiP32905. 155 interactions.
MINTiMINT-562708.
STRINGi4932.YGR214W.

Structurei

Secondary structure

1
252
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2010
Turni21 – 233
Helixi31 – 366
Beta strandi37 – 415
Turni42 – 443
Beta strandi45 – 484
Helixi50 – 6415
Beta strandi67 – 693
Helixi70 – 723
Beta strandi73 – 775
Helixi80 – 9213
Beta strandi96 – 1005
Beta strandi106 – 1083
Beta strandi119 – 1246
Turni126 – 1294
Helixi130 – 1378
Turni138 – 1403
Beta strandi143 – 1475
Beta strandi157 – 1615
Helixi169 – 18214
Beta strandi183 – 1875
Beta strandi190 – 1923
Helixi199 – 2013

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-B14-198[»]
1S1Helectron microscopy11.70B14-198[»]
1VW9electron microscopy6.10B1-252[»]
1VWVelectron microscopy6.10B1-252[»]
1VXWelectron microscopy6.20A1-252[»]
1VXZelectron microscopy6.30A1-252[»]
3IZBelectron microscopy-A1-252[»]
3O2ZX-ray4.00A1-252[»]
3O30X-ray4.00A1-252[»]
3U5CX-ray3.00A1-252[»]
3U5GX-ray3.00A1-252[»]
4BYLelectron microscopy4.30A1-252[»]
4BYTelectron microscopy6.60A1-252[»]
4CUYelectron microscopy3.70A2-207[»]
ProteinModelPortaliP32905.
SMRiP32905. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32905.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S2P family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015036.
HOGENOMiHOG000232073.
InParanoidiP32905.
KOiK02998.
OMAiCHYQMER.
OrthoDBiEOG741ZD0.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
InterProiIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32905-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV
60 70 80 90 100
GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKFA AHTGATPIAG
110 120 130 140 150
RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD
160 170 180 190 200
SPSEFVDVAI PCNNRGKHSI GLIWYLLARE VLRLRGALVD RTQPWSIMPD
210 220 230 240 250
LYFYRDPEEV EQQVAEEATT EEAGEEEAKE EVTEEQAEAT EWAEENADNV

EW
Length:252
Mass (Da):28,024
Last modified:January 23, 2007 - v3
Checksum:iD6F263456282C771
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M88277 Genomic DNA. Translation: AAB05643.1.
Z72999 Genomic DNA. Translation: CAA97241.1.
BK006941 Genomic DNA. Translation: DAA08308.1.
PIRiS42143.
RefSeqiNP_011730.1. NM_001181343.1.

Genome annotation databases

EnsemblFungiiYGR214W; YGR214W; YGR214W.
GeneIDi853128.
KEGGisce:YGR214W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M88277 Genomic DNA. Translation: AAB05643.1 .
Z72999 Genomic DNA. Translation: CAA97241.1 .
BK006941 Genomic DNA. Translation: DAA08308.1 .
PIRi S42143.
RefSeqi NP_011730.1. NM_001181343.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5X model - B 14-198 [» ]
1S1H electron microscopy 11.70 B 14-198 [» ]
1VW9 electron microscopy 6.10 B 1-252 [» ]
1VWV electron microscopy 6.10 B 1-252 [» ]
1VXW electron microscopy 6.20 A 1-252 [» ]
1VXZ electron microscopy 6.30 A 1-252 [» ]
3IZB electron microscopy - A 1-252 [» ]
3O2Z X-ray 4.00 A 1-252 [» ]
3O30 X-ray 4.00 A 1-252 [» ]
3U5C X-ray 3.00 A 1-252 [» ]
3U5G X-ray 3.00 A 1-252 [» ]
4BYL electron microscopy 4.30 A 1-252 [» ]
4BYT electron microscopy 6.60 A 1-252 [» ]
4CUY electron microscopy 3.70 A 2-207 [» ]
ProteinModelPortali P32905.
SMRi P32905. Positions 2-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33467. 102 interactions.
DIPi DIP-5553N.
IntActi P32905. 155 interactions.
MINTi MINT-562708.
STRINGi 4932.YGR214W.

2D gel databases

SWISS-2DPAGE P32905.

Proteomic databases

MaxQBi P32905.
PeptideAtlasi P32905.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR214W ; YGR214W ; YGR214W .
GeneIDi 853128.
KEGGi sce:YGR214W.

Organism-specific databases

SGDi S000003446. RPS0A.

Phylogenomic databases

GeneTreei ENSGT00390000015036.
HOGENOMi HOG000232073.
InParanoidi P32905.
KOi K02998.
OMAi CHYQMER.
OrthoDBi EOG741ZD0.

Enzyme and pathway databases

BioCyci YEAST:G3O-30896-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

EvolutionaryTracei P32905.
NextBioi 973178.

Gene expression databases

Genevestigatori P32905.

Family and domain databases

HAMAPi MF_03015. Ribosomal_S2_euk.
InterProi IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view ]
PANTHERi PTHR11489. PTHR11489. 1 hit.
Pfami PF00318. Ribosomal_S2. 2 hits.
[Graphical view ]
PRINTSi PR00395. RIBOSOMALS2.
SUPFAMi SSF52313. SSF52313. 1 hit.
TIGRFAMsi TIGR01012. Sa_S2_E_A. 1 hit.
PROSITEi PS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
    Demianova M.A., Formosa T.G., Ellis S.R.
    J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: PROTEIN SEQUENCE OF 118-133 AND 231-248.
    Strain: ATCC 204508 / S288c.
  6. "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
    Norbeck J., Blomberg A.
    J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-36 AND 135-146.
    Strain: ATCC 44827 / SKQ2N.
  7. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.
  8. "The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
    Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
    Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE EVOLUTION.
  9. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  10. "Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
    Ford C.L., Randal-Whitis L., Ellis S.R.
    Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  11. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
    Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
    Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  15. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
  16. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.

Entry informationi

Entry nameiRSSA1_YEAST
AccessioniPrimary (citable) accession number: P32905
Secondary accession number(s): D6VUZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 93700 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S0 in yeast.
This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3