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P32905 (RSSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S0-A
Alternative name(s):
Nucleic acid-binding protein NAB1A
Gene names
Name:RPS0A
Synonyms:NAB1, NAB1A, YST1
Ordered Locus Names:YGR214W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Ref.10 Ref.14

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.9 Ref.10

Subcellular location

Cytoplasm Ref.12 Ref.14.

Post-translational modification

N-terminally acetylated by acetyltransferase NatA. Ref.7 Ref.11

Disruption phenotype

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size. Ref.14

Miscellaneous

Present with 93700 molecules/cell in log phase SD medium.

There are 2 genes for S0 in yeast.

This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

Sequence similarities

Belongs to the ribosomal protein S2P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 25225140S ribosomal protein S0-A HAMAP-Rule MF_03015
PRO_0000134370

Amino acid modifications

Modified residue21N-acetylserine Ref.7 Ref.11

Secondary structure

...................................... 252
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32905 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: D6F263456282C771

FASTA25228,024
        10         20         30         40         50         60 
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA 

        70         80         90        100        110        120 
ARIIAAIPNP EDVVAISSRT FGQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL 

       130        140        150        160        170        180 
VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI PCNNRGKHSI GLIWYLLARE 

       190        200        210        220        230        240 
VLRLRGALVD RTQPWSIMPD LYFYRDPEEV EQQVAEEATT EEAGEEEAKE EVTEEQAEAT 

       250 
EWAEENADNV EW 

« Hide

References

« Hide 'large scale' references
[1]"Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
Demianova M.A., Formosa T.G., Ellis S.R.
J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Protein identifications for a Saccharomyces cerevisiae protein database."
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
Electrophoresis 15:1466-1486(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 118-133 AND 231-248.
Strain: ATCC 204508 / S288c.
[6]"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
Norbeck J., Blomberg A.
J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-36 AND 135-146.
Strain: ATCC 44827 / SKQ2N.
[7]"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT SER-2.
[8]"The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE EVOLUTION.
[9]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[10]"Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
Ford C.L., Randal-Whitis L., Ellis S.R.
Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[11]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[12]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[15]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
[16]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M88277 Genomic DNA. Translation: AAB05643.1.
Z72999 Genomic DNA. Translation: CAA97241.1.
BK006941 Genomic DNA. Translation: DAA08308.1.
PIRS42143.
RefSeqNP_011730.1. NM_001181343.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-B14-198[»]
1S1Helectron microscopy11.70B14-198[»]
3IZBelectron microscopy-A1-252[»]
3O2ZX-ray4.00A1-252[»]
3O30X-ray4.00A1-252[»]
3U5CX-ray3.00A1-252[»]
3U5GX-ray3.00A1-252[»]
4BYLelectron microscopy4.30A1-252[»]
4BYTelectron microscopy6.60A1-252[»]
ProteinModelPortalP32905.
SMRP32905. Positions 2-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33467. 100 interactions.
DIPDIP-5553N.
IntActP32905. 155 interactions.
MINTMINT-562708.
STRING4932.YGR214W.

2D gel databases

SWISS-2DPAGEP32905.

Proteomic databases

PeptideAtlasP32905.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR214W; YGR214W; YGR214W.
GeneID853128.
KEGGsce:YGR214W.

Organism-specific databases

SGDS000003446. RPS0A.

Phylogenomic databases

GeneTreeENSGT00390000015036.
HOGENOMHOG000232073.
KOK02998.
OMATQQDIEM.
OrthoDBEOG741ZD0.

Enzyme and pathway databases

BioCycYEAST:G3O-30896-MONOMER.

Gene expression databases

GenevestigatorP32905.

Family and domain databases

HAMAPMF_03015. Ribosomal_S2_euk.
InterProIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERPTHR11489. PTHR11489. 1 hit.
PfamPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSPR00395. RIBOSOMALS2.
SUPFAMSSF52313. SSF52313. 1 hit.
TIGRFAMsTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32905.
NextBio973178.

Entry information

Entry nameRSSA1_YEAST
AccessionPrimary (citable) accession number: P32905
Secondary accession number(s): D6VUZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references