40S ribosomal protein S0-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32905 (RSSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S0-A

Alternative name(s):
Nucleic acid-binding protein NAB1A

Gene names

Name:	RPS0A
Synonyms:	NAB1, NAB1A, YST1
Ordered Locus Names:	YGR214W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Ref.10 Ref.14
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.9 Ref.10
Subcellular location	Cytoplasm Ref.12 Ref.14.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Disruption phenotype	Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size. Ref.14
Miscellaneous	Present with 93700 molecules/cell in log phase SD medium. Ref.13 There are 2 genes for S0 in yeast. This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.
Sequence similarities	Belongs to the ribosomal protein S2P family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred from mutant phenotype Ref.1 Ref.10. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.14. Source: SGD endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.14. Source: SGD rRNA export from nucleus Inferred from genetic interaction. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay Ref.1. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.11

Chain

2 – 252

251

40S ribosomal protein S0-A

PRO_0000134370

Amino acid modifications

Modified residue

N-acetylserine Ref.7 Ref.11

Modified residue

103

Phosphothreonine Ref.16

Modified residue

112

Phosphothreonine Ref.15 Ref.16

Sequences

Sequence

Length

Mass (Da)

Tools

P32905 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: D6F263456282C771
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FASTA

252

28,024

        10         20         30         40         50         60 
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA 

        70         80         90        100        110        120 
ARIIAAIPNP EDVVAISSRT FGQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL 

       130        140        150        160        170        180 
VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI PCNNRGKHSI GLIWYLLARE 

       190        200        210        220        230        240 
VLRLRGALVD RTQPWSIMPD LYFYRDPEEV EQQVAEEATT EEAGEEEAKE EVTEEQAEAT 

       250 
EWAEENADNV EW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit." Demianova M.A., Formosa T.G., Ellis S.R. J. Biol. Chem. 271:11383-11391(1996) [PubMed: 8626693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Protein identifications for a Saccharomyces cerevisiae protein database." Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S. Electrophoresis 15:1466-1486(1994) [PubMed: 7895733] [Abstract] Cited for: PROTEIN SEQUENCE OF 118-133 AND 231-248. Strain: ATCC 204508 / S288c.
[6]	"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway." Norbeck J., Blomberg A. J. Biol. Chem. 272:5544-5554(1997) [PubMed: 9038161] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-36 AND 135-146. Strain: ATCC 44827 / SKQ2N.
[7]	"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2.
[8]	"The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution." Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S. Mol. Biol. Evol. 15:1017-1025(1998) [PubMed: 9718729] [Abstract] Cited for: GENE EVOLUTION.
[9]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[10]	"Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits." Ford C.L., Randal-Whitis L., Ellis S.R. Cancer Res. 59:704-710(1999) [PubMed: 9973221] [Abstract] Cited for: FUNCTION, SUBUNIT.
[11]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[12]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]	"Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA." Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R. Nucleic Acids Res. 31:6798-6805(2003) [PubMed: 14627813] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[15]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-112, MASS SPECTROMETRY. Strain: YAL6B.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-103 AND THR-112, MASS SPECTROMETRY.
[17]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
[18]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 14-198, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M88277 Genomic DNA. Translation: AAB05643.1.
Z72999 Genomic DNA. Translation: CAA97241.1.
BK006941 Genomic DNA. Translation: DAA08308.1.

PIR

S42143.

RefSeq

NP_011730.1. NM_001181343.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	B	14-198	[»]
1S1H	electron microscopy	11.70	B	14-198	[»]
3IZB	electron microscopy	-	A	1-252	[»]
3O2Z	X-ray	4.00	A	1-252	[»]
3O30	X-ray	4.00	A	1-252	[»]

ProteinModelPortal

P32905.

SMR

P32905. Positions 9-201.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5553N.

IntAct

P32905. 154 interactions.

MINT

MINT-562708.

STRING

P32905.

2D gel databases

SWISS-2DPAGE

P32905.

Proteomic databases

PeptideAtlas

P32905.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR214W; YGR214W; YGR214W.

GeneID

853128.

KEGG

sce:YGR214W.

NMPDR

fig|4932.3.peg.2858.

Organism-specific databases

SGD

S000003446. RPS0A.

Phylogenomic databases

eggNOG

fuNOG05580.

GeneTree

EFGT00050000005275.

HOGENOM

HBG736296.

OMA

MAVTRRV.

OrthoDB

EOG4KH646.

Gene expression databases

ArrayExpress

P32905.

Genevestigator

P32905.

GermOnline

YGR214W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]

K02998.

PANTHER

PTHR11489. Ribosomal_S2_e/a. 1 hit.

Pfam

PF00318. Ribosomal_S2. 2 hits.
[Graphical view]

PRINTS

PR00395. RIBOSOMALS2.

SUPFAM

SSF52313. Ribosomal_S2. 1 hit.

TIGRFAMs

TIGR01012. Sa_S2_E_A. 1 hit.

PROSITE

PS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

973178.

Entry information

Entry name

RSSA1_YEAST

Accession

Primary (citable) accession number: P32905
Secondary accession number(s): D6VUZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents