Reviewed, UniProtKB/Swiss-Prot P32902 (RT04_YEAST)

Names and origin

Protein names

Recommended name:
    37S ribosomal protein MRP4, mitochondrial

Gene names

Name:	MRP4
Synonyms:	MRP4A
Ordered Locus Names:	YHL004W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).
Subcellular location	Mitochondrion. Ref.6 Ref.8
Miscellaneous	Present with 6890 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the ribosomal protein S2P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial translation Ref.4 Inferred by curator. Source: SGD
Cellular component	mitochondrial small ribosomal subunit Ref.4 Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Ref.4 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG17	Q06410	1	EBI-16248,EBI-30856
BBP1	Q12365	1	EBI-16248,EBI-3448
GET3	Q12154	1	EBI-16248,EBI-2989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Potential
	Chain	26 – 394	369	37S ribosomal protein MRP4, mitochondrial		PRO_0000134345

Sequences

	Sequence		Length	Mass (Da)
	P32902-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 81A1CB33134BC559	FASTA	394	44,152
	10 20 30 40 50 60 MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS 70 80 90 100 110 120 LSQVPEDEIL PELKKSLEQD KLSDKEQQLE AELSDFFRNY ALLNKLFDSK TLDGQSSTTT 130 140 150 160 170 180 AAATPTKPYP NLIPSANDKP YSSQELFLRQ LNHSMRTAKL GATISKVYYP HKDIFYPPLP 190 200 210 220 230 240 ENITVESLMS AGVHLGQSTS LWRSSTQSYI YGEYKGIHII DLNQTLSYLK RAAKVVEGVS 250 260 270 280 290 300 ESGGIILFLG TRQGQKRGLE EAAKKTHGYY VSTRWIPGTL TNSTEISGIW EKQEIDSNDN 310 320 330 340 350 360 PTERALSPNE TSKQVKPDLL VVLNPTENRN ALLEAIKSRV PTIAIIDTDS EPSLVTYPIP 370 380 390 GNDDSLRSVN FLLGVLARAG QRGLQNRLAR NNEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a yeast mitochondrial ribosomal protein structurally related to the mammalian 68-kDa high affinity laminin receptor." Davis S.C., Tzagoloff A., Ellis S.R. J. Biol. Chem. 267:5508-5514(1992) [PubMed: 1531984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues." Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A., Jacquier A. J. Biol. Chem. 276:15861-15867(2001) [PubMed: 11278769] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[5]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed: 12392552] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M82841 Genomic DNA. Translation: AAA34793.1. U10555 Genomic DNA. Translation: AAB68428.1. AY558385 Genomic DNA. Translation: AAS56711.1.
PIR	A42115.
RefSeq	NP_011859.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5035N.
IntAct	P32902. 72 interactions.
Proteomic databases
PeptideAtlas	P32902.
PRIDE	P32902.
Genome annotation databases
Ensembl	YHL004W. Saccharomyces cerevisiae. [Contig view]
GeneID	856384.
GenomeReviews	Gene locus YHL004W in contig U00093_GR.
KEGG	sce:YHL004W.
NMPDR	fig|4932.3.peg.3002.
Organism-specific databases
CYGD	YHL004w.
SGD	S000000996. MRP4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32902.
OMA	P32902. LNPTENR.
Gene expression databases
ArrayExpress	P32902.
GermOnline	YHL004W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001865. Ribosomal_S2. IPR005706. Ribosomal_S2_bac-type. IPR018130. Ribosomal_S2_CS. [Graphical view]
PANTHER	PTHR12534. Ribosom_S2_bac. 1 hit.
Pfam	PF00318. Ribosomal_S2. 1 hit. [Graphical view]
PRINTS	PR00395. RIBOSOMALS2.
TIGRFAMs	TIGR01011. rpsB_bact. 1 hit.
PROSITE	PS00962. RIBOSOMAL_S2_1. 1 hit. PS00963. RIBOSOMAL_S2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981887.

Entry information

Entry name

RT04_YEAST

Accession

Primary (citable) accession number: P32902

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)