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Protein

Mitochondrial presequence protease

Gene

CYM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-independent protease that degrades mitochondrial transit peptides after their cleavage both in intermembrane space and in matrix. Also degrades other unstructured peptides (By similarity).By similarity1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Zinc; catalytic
Active sitei87 – 871Proton acceptor
Metal bindingi88 – 881Zinc; catalytic
Metal bindingi185 – 1851Zinc; catalyticBy similarity

GO - Molecular functioni

  • metalloendopeptidase activity Source: SGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • protein processing Source: GO_Central
  • proteolysis Source: SGD
  • proteolysis involved in cellular protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29969-MONOMER.

Protein family/group databases

MEROPSiM16.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial presequence protease (EC:3.4.24.-)
Alternative name(s):
Cytosolic metalloprotease 1
Metalloprotease of 112 kDa
Gene namesi
Name:CYM1
Synonyms:MOP112
Ordered Locus Names:YDR430C
ORF Names:D9461.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR430C.
SGDiS000002838. CYM1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841H → Y: Loss of function. 1 Publication
Mutagenesisi87 – 871E → Q: Loss of function. 1 Publication
Mutagenesisi88 – 881H → Y: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 989989Mitochondrial presequence proteasePRO_0000178012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei920 – 9201PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32898.

PTM databases

iPTMnetiP32898.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32488. 34 interactions.
DIPiDIP-6735N.
IntActiP32898. 7 interactions.
MINTiMINT-626244.

Structurei

3D structure databases

ProteinModelPortaliP32898.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family. PreP subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018381.
HOGENOMiHOG000008829.
InParanoidiP32898.
KOiK06972.
OMAiLYAMQNA.
OrthoDBiEOG092C0AF6.

Family and domain databases

Gene3Di3.30.830.10. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR007863. Peptidase_M16_C.
IPR013578. Peptidase_M16C_assoc.
[Graphical view]
PfamiPF08367. M16C_assoc. 1 hit.
PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 2 hits.
[Graphical view]
SMARTiSM01264. M16C_associated. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 4 hits.

Sequencei

Sequence statusi: Complete.

P32898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFQRFASS YAQAQAVRKY PVGGIFHGYE VRRILPVPEL RLTAVDLVHS
60 70 80 90 100
QTGAEHLHID RDDKNNVFSI AFKTNPPDST GVPHILEHTT LCGSVKYPVR
110 120 130 140 150
DPFFKMLNKS LANFMNAMTG PDYTFFPFST TNPQDFANLR GVYLDSTLNP
160 170 180 190 200
LLKQEDFDQE GWRLEHKNIT DPESNIVFKG VVYNEMKGQI SNANYYFWSK
210 220 230 240 250
FQQSIYPSLN NSGGDPMKIT DLRYGDLLDF HHKNYHPSNA KTFTYGNLPL
260 270 280 290 300
VDTLKQLNEQ FSGYGKRARK DKLLMPIDLK KDIDVKLLGQ IDTMLPPEKQ
310 320 330 340 350
TKASMTWICG APQDTYDTFL LKVLGNLLMD GHSSVMYQKL IESGIGLEFS
360 370 380 390 400
VNSGVEPTTA VNLLTVGIQG VSDIEIFKDT VNNIFQNLLE TEHPFDRKRI
410 420 430 440 450
DAIIEQLELS KKDQKADFGL QLLYSILPGW TNKIDPFESL LFEDVLQRFR
460 470 480 490 500
GDLETKGDTL FQDLIRKYIV HKPCFTFSIQ GSEEFSKSLD DEEQTRLREK
510 520 530 540 550
ITALDEQDKK NIFKRGILLQ EKQNEKEDLS CLPTLQIKDI PRAGDKYSIE
560 570 580 590 600
QKNNTMSRIT DTNGITYVRG KRLLNDIIPF ELFPYLPLFA ESLTNLGTTT
610 620 630 640 650
ESFSEIEDQI KLHTGGISTH VEVTSDPNTT EPRLIFGFDG WSLNSKTDHI
660 670 680 690 700
FEFWSKILLE TDFHKNSDKL KVLIRLLASS NTSSVADAGH AFARGYSAAH
710 720 730 740 750
YRSSGAINET LNGIEQLQFI NRLHSLLDNE ETFQREVVDK LTELQKYIVD
760 770 780 790 800
TNNMNFFITS DSDVQAKTVE SQISKFMERL PHGSCLPNGP KTSDYPLIGS
810 820 830 840 850
KCKHTLIKFP FQVHYTSQAL LGVPYTHKDG SALQVMSNML TFKHLHREVR
860 870 880 890 900
EKGGAYGGGA SYSALAGIFS FYSYRDPQPL KSLETFKNSG RYILNDAKWG
910 920 930 940 950
VTDLDEAKLT IFQQVDAPKS PKGEGVTYFM SGVTDDMKQA RREQLLDVSL
960 970 980
LDVHRVAEKY LLNKEGVSTV IGPGIEGKTV SPNWEVKEL
Length:989
Mass (Da):112,180
Last modified:December 15, 1998 - v2
Checksum:i6CAD2EE5A6080D9C
GO

Sequence cautioni

The sequence AAB64877 differs from that shown. Reason: Frameshift at position 18. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70951 Genomic DNA. Translation: CAA50290.1.
U33007 Genomic DNA. Translation: AAB64877.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12269.1.
PIRiS69711.
RefSeqiNP_010718.1. NM_001180738.1.

Genome annotation databases

EnsemblFungiiYDR430C; YDR430C; YDR430C.
GeneIDi852041.
KEGGisce:YDR430C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70951 Genomic DNA. Translation: CAA50290.1.
U33007 Genomic DNA. Translation: AAB64877.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12269.1.
PIRiS69711.
RefSeqiNP_010718.1. NM_001180738.1.

3D structure databases

ProteinModelPortaliP32898.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32488. 34 interactions.
DIPiDIP-6735N.
IntActiP32898. 7 interactions.
MINTiMINT-626244.

Protein family/group databases

MEROPSiM16.013.

PTM databases

iPTMnetiP32898.

Proteomic databases

MaxQBiP32898.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR430C; YDR430C; YDR430C.
GeneIDi852041.
KEGGisce:YDR430C.

Organism-specific databases

EuPathDBiFungiDB:YDR430C.
SGDiS000002838. CYM1.

Phylogenomic databases

GeneTreeiENSGT00390000018381.
HOGENOMiHOG000008829.
InParanoidiP32898.
KOiK06972.
OMAiLYAMQNA.
OrthoDBiEOG092C0AF6.

Enzyme and pathway databases

BioCyciYEAST:G3O-29969-MONOMER.

Miscellaneous databases

PROiP32898.

Family and domain databases

Gene3Di3.30.830.10. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR007863. Peptidase_M16_C.
IPR013578. Peptidase_M16C_assoc.
[Graphical view]
PfamiPF08367. M16C_assoc. 1 hit.
PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 2 hits.
[Graphical view]
SMARTiSM01264. M16C_associated. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCYM1_YEAST
AccessioniPrimary (citable) accession number: P32898
Secondary accession number(s): D6VT59, Q04068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.