Reviewed, UniProtKB/Swiss-Prot P32897 (TIM23_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM23
Alternative name(s):
    Mitochondrial protein import protein 3
    Mitochondrial protein import protein MAS6
    Membrane import machinery protein MIM23

Gene names

Name:	TIM23
Synonyms:	MAS6, MIM23, MPI3
Ordered Locus Names:	YNR017W
ORF Names:	N3180

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Subunit structure	Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein. Note: Around 20 amino acids of the N-terminus are believed to span the mitochondrial outer membrane. This association is dynamic and depends on the translocation activity of the TIM23 complex. However, this topology seems not to be critical for formation of a TOM-TIM supercomplex and preprotein import. Ref.8
Miscellaneous	Present with 1440 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the Tim17/Tim22/Tim23 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial matrix Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane presequence translocase complex Inferred from physical interaction. Source: SGD
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro mitochondrion targeting sequence binding Inferred from direct assay. Source: SGD protein channel activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TIM21	P53220	2	EBI-9136,EBI-23128
TIM50	Q02776	1	EBI-9136,EBI-30302

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Mitochondrial import inner membrane translocase subunit TIM23		PRO_0000210307
Regions
	Transmembrane	1 – ?		Outer membrane Potential
	Topological domain	? – 96		Mitochondrial intermembrane Potential
	Transmembrane	97 – 118	22	Inner membrane Potential
	Topological domain	119 – 144	26	Mitochondrial matrix Potential
	Transmembrane	145 – 166	22	Inner membrane Potential
	Topological domain	167 – 174	8	Mitochondrial intermembrane Potential
	Transmembrane	175 – 189	15	Inner membrane Potential
	Topological domain	190 – 196	7	Mitochondrial matrix Potential
	Transmembrane	197 – 215	19	Inner membrane Potential
	Topological domain	216 – 222	7	Mitochondrial intermembrane Potential

Sequences

	Sequence		Length	Mass (Da)
	P32897-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 08E5AB4F400F675B	FASTA	222	23,244
	10 20 30 40 50 60 MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG MHVDTARLHP 70 80 90 100 110 120 LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG TGAVYLLGLG IGGFSGMMQG 130 140 150 160 170 180 LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN NAGILALSYN IINSTIDALR GKHDTAGSIG 190 200 210 220 AGALTGALFK SSKGLKPMGY SSAMVAAACA VWCSVKKRLL EK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway." Emtage J.L.T., Jensen R.E. J. Cell Biol. 122:1003-1012(1993) [PubMed: 8354690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane." Dekker P.J.T., Keil P., Rassow J., Maarse A.C., Pfanner N., Meijer M. FEBS Lett. 330:66-70(1993) [PubMed: 8370462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site." Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N. FEBS Lett. 349:222-228(1994) [PubMed: 8050570] [Abstract] Cited for: CHARACTERIZATION, TOPOLOGY.
[5]	"Tim23 links the inner and outer mitochondrial membranes." Donzeau M., Kaldi K., Adam A., Paschen S., Wanner G., Guiard B., Bauer M.F., Neupert W., Brunner M. Cell 101:401-412(2000) [PubMed: 10830167] [Abstract] Cited for: TOPOLOGY.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17." Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P. Cell 120:817-829(2005) [PubMed: 15797382] [Abstract] Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
[8]	"Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria." Popov-Celeketic D., Mapa K., Neupert W., Mokranjac D. EMBO J. 27:1469-1480(2008) [PubMed: 18418384] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71633 Genomic DNA. Translation: CAA50640.1. X74161 Genomic DNA. Translation: CAA52274.1. Z71632 Genomic DNA. Translation: CAA96296.1.
PIR	S36139.
RefSeq	NP_014414.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2451N.
IntAct	P32897. 8 interactions.
STRING	P32897.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PeptideAtlas	P32897.
Genome annotation databases
Ensembl	YNR017W; YNR017W; YNR017W; Saccharomyces cerevisiae. [Genome view]
GeneID	855751.
KEGG	sce:YNR017W.
NMPDR	fig|4932.3.peg.5494.
Organism-specific databases
CYGD	YNR017w.
SGD	S000005300. MAS6.
Phylogenomic databases
HOGENOM	HBG396465.
OMA	GPFLGNS.
OrthoDB	EOG9869VV.
Gene expression databases
ArrayExpress	P32897.
Genevestigator	P32897.
GermOnline	YNR017W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003397. Tim17/Tim22. IPR005681. Tim23. [Graphical view]
Pfam	PF02466. Tim17. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980166.

Entry information

Entry name

TIM23_YEAST

Accession

Primary (citable) accession number: P32897

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 15, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)