SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32897

- TIM23_YEAST

UniProt

P32897 - TIM23_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Mitochondrial import inner membrane translocase subunit TIM23
Gene
TIM23, MAS6, MIM23, MPI3, YNR017W, N3180
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

GO - Molecular functioni

  1. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  2. mitochondrion targeting sequence binding Source: SGD
  3. protein binding Source: IntAct
  4. protein channel activity Source: SGD

GO - Biological processi

  1. protein import into mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33332-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM23
Alternative name(s):
Membrane import machinery protein MIM23
Mitochondrial protein import protein 3
Mitochondrial protein import protein MAS6
Gene namesi
Name:TIM23
Synonyms:MAS6, MIM23, MPI3
Ordered Locus Names:YNR017W
ORF Names:N3180
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR017w.
SGDiS000005300. TIM23.

Subcellular locationi

Mitochondrion inner membrane; Multi-pass membrane protein
Note: Around 20 amino acids of the N-terminus are believed to span the mitochondrial outer membrane. This association is dynamic and depends on the translocation activity of the TIM23 complex. However, this topology seems not to be critical for formation of a TOM-TIM supercomplex and preprotein import.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini? – 96Mitochondrial intermembrane Reviewed prediction
Transmembranei1 – ?Helical; Note=Outer membrane; Reviewed prediction
Transmembranei97 – 11822Helical; Note=Inner membrane; Reviewed prediction
Add
BLAST
Topological domaini119 – 14426Mitochondrial matrix Reviewed prediction
Add
BLAST
Transmembranei145 – 16622Helical; Note=Inner membrane; Reviewed prediction
Add
BLAST
Topological domaini167 – 1748Mitochondrial intermembrane Reviewed prediction
Transmembranei175 – 18915Helical; Note=Inner membrane; Reviewed prediction
Add
BLAST
Topological domaini190 – 1967Mitochondrial matrix Reviewed prediction
Transmembranei197 – 21519Helical; Note=Inner membrane; Reviewed prediction
Add
BLAST
Topological domaini216 – 2227Mitochondrial intermembrane Reviewed prediction

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: Reactome
  3. mitochondrial inner membrane presequence translocase complex Source: SGD
  4. mitochondrial intermembrane space Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Mitochondrial import inner membrane translocase subunit TIM23
PRO_0000210307Add
BLAST

Proteomic databases

MaxQBiP32897.
PaxDbiP32897.
PeptideAtlasiP32897.

Expressioni

Gene expression databases

GenevestigatoriP32897.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PAM16P429496EBI-9136,EBI-26019
TIM17P3951513EBI-9136,EBI-9127
TIM21P5322012EBI-9136,EBI-23128
TIM44Q018528EBI-9136,EBI-9141
TIM50Q027769EBI-9136,EBI-30302

Protein-protein interaction databases

BioGridi35842. 50 interactions.
DIPiDIP-2451N.
IntActiP32897. 15 interactions.
MINTiMINT-1359281.
STRINGi4932.YNR017W.

Structurei

3D structure databases

ProteinModelPortaliP32897.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim17/Tim22/Tim23 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5596.
HOGENOMiHOG000166435.
KOiK17794.
OMAiHITKRGP.
OrthoDBiEOG75MW7H.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
IPR005681. Tim23.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00983. 3a0801s02tim23. 1 hit.

Sequencei

Sequence statusi: Complete.

P32897-1 [UniParc]FASTAAdd to Basket

« Hide

MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG    50
MHVDTARLHP LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG 100
TGAVYLLGLG IGGFSGMMQG LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN 150
NAGILALSYN IINSTIDALR GKHDTAGSIG AGALTGALFK SSKGLKPMGY 200
SSAMVAAACA VWCSVKKRLL EK 222
Length:222
Mass (Da):23,244
Last modified:October 1, 1993 - v1
Checksum:i08E5AB4F400F675B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X71633 Genomic DNA. Translation: CAA50640.1.
X74161 Genomic DNA. Translation: CAA52274.1.
Z71632 Genomic DNA. Translation: CAA96296.1.
BK006947 Genomic DNA. Translation: DAA10558.1.
PIRiS36139.
RefSeqiNP_014414.3. NM_001183194.3.

Genome annotation databases

EnsemblFungiiYNR017W; YNR017W; YNR017W.
GeneIDi855751.
KEGGisce:YNR017W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X71633 Genomic DNA. Translation: CAA50640.1 .
X74161 Genomic DNA. Translation: CAA52274.1 .
Z71632 Genomic DNA. Translation: CAA96296.1 .
BK006947 Genomic DNA. Translation: DAA10558.1 .
PIRi S36139.
RefSeqi NP_014414.3. NM_001183194.3.

3D structure databases

ProteinModelPortali P32897.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35842. 50 interactions.
DIPi DIP-2451N.
IntActi P32897. 15 interactions.
MINTi MINT-1359281.
STRINGi 4932.YNR017W.

Chemistry

BindingDBi P32897.
ChEMBLi CHEMBL1741180.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi P32897.
PaxDbi P32897.
PeptideAtlasi P32897.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR017W ; YNR017W ; YNR017W .
GeneIDi 855751.
KEGGi sce:YNR017W.

Organism-specific databases

CYGDi YNR017w.
SGDi S000005300. TIM23.

Phylogenomic databases

eggNOGi COG5596.
HOGENOMi HOG000166435.
KOi K17794.
OMAi HITKRGP.
OrthoDBi EOG75MW7H.

Enzyme and pathway databases

BioCyci YEAST:G3O-33332-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 980166.
PROi P32897.

Gene expression databases

Genevestigatori P32897.

Family and domain databases

InterProi IPR003397. Tim17/Tim22/Tim23/PMP24.
IPR005681. Tim23.
[Graphical view ]
Pfami PF02466. Tim17. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00983. 3a0801s02tim23. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway."
    Emtage J.L.T., Jensen R.E.
    J. Cell Biol. 122:1003-1012(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane."
    Dekker P.J.T., Keil P., Rassow J., Maarse A.C., Pfanner N., Meijer M.
    FEBS Lett. 330:66-70(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
    Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
    FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, TOPOLOGY.
  6. Cited for: TOPOLOGY.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
    Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
    Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
  9. "Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria."
    Popov-Celeketic D., Mapa K., Neupert W., Mokranjac D.
    EMBO J. 27:1469-1480(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.

Entry informationi

Entry nameiTIM23_YEAST
AccessioniPrimary (citable) accession number: P32897
Secondary accession number(s): D6W1J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1440 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi