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Protein

Mitochondrial import inner membrane translocase subunit TIM23

Gene

TIM23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Miscellaneous

Present with 1440 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mitochondrion targeting sequence binding Source: SGD
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  • protein channel activity Source: SGD

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
  • protein targeting to mitochondrion Source: Reactome

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33332-MONOMER.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM23
Alternative name(s):
Membrane import machinery protein MIM23
Mitochondrial protein import protein 3
Mitochondrial protein import protein MAS6
Gene namesi
Name:TIM23
Synonyms:MAS6, MIM23, MPI3
Ordered Locus Names:YNR017W
ORF Names:N3180
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR017W.
SGDiS000005300. TIM23.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 96Mitochondrial intermembraneSequence analysis
Transmembranei1 – ?Helical; Note=Outer membraneSequence analysis
Transmembranei97 – 118Helical; Note=Inner membraneSequence analysisAdd BLAST22
Topological domaini119 – 144Mitochondrial matrixSequence analysisAdd BLAST26
Transmembranei145 – 166Helical; Note=Inner membraneSequence analysisAdd BLAST22
Topological domaini167 – 174Mitochondrial intermembraneSequence analysis8
Transmembranei175 – 189Helical; Note=Inner membraneSequence analysisAdd BLAST15
Topological domaini190 – 196Mitochondrial matrixSequence analysis7
Transmembranei197 – 215Helical; Note=Inner membraneSequence analysisAdd BLAST19
Topological domaini216 – 222Mitochondrial intermembraneSequence analysis7

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103071 – 222Mitochondrial import inner membrane translocase subunit TIM23Add BLAST222

Proteomic databases

MaxQBiP32897.
PRIDEiP32897.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35842. 336 interactors.
DIPiDIP-2451N.
IntActiP32897. 16 interactors.
MINTiMINT-1359281.
STRINGi4932.YNR017W.

Chemistry databases

BindingDBiP32897.

Structurei

3D structure databases

ProteinModelPortaliP32897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim17/Tim22/Tim23 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000166435.
InParanoidiP32897.
KOiK17794.
OMAiSGMLFKS.
OrthoDBiEOG092C5C6F.

Family and domain databases

InterProiView protein in InterPro
IPR003397. Tim17/Tim22/Tim23/PMP24.
IPR005681. Tim23.
PfamiView protein in Pfam
PF02466. Tim17. 1 hit.
TIGRFAMsiTIGR00983. 3a0801s02tim23. 1 hit.

Sequencei

Sequence statusi: Complete.

P32897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG
60 70 80 90 100
MHVDTARLHP LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG
110 120 130 140 150
TGAVYLLGLG IGGFSGMMQG LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN
160 170 180 190 200
NAGILALSYN IINSTIDALR GKHDTAGSIG AGALTGALFK SSKGLKPMGY
210 220
SSAMVAAACA VWCSVKKRLL EK
Length:222
Mass (Da):23,244
Last modified:October 1, 1993 - v1
Checksum:i08E5AB4F400F675B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71633 Genomic DNA. Translation: CAA50640.1.
X74161 Genomic DNA. Translation: CAA52274.1.
Z71632 Genomic DNA. Translation: CAA96296.1.
BK006947 Genomic DNA. Translation: DAA10558.1.
PIRiS36139.
RefSeqiNP_014414.3. NM_001183194.3.

Genome annotation databases

EnsemblFungiiYNR017W; YNR017W; YNR017W.
GeneIDi855751.
KEGGisce:YNR017W.

Similar proteinsi

Entry informationi

Entry nameiTIM23_YEAST
AccessioniPrimary (citable) accession number: P32897
Secondary accession number(s): D6W1J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 22, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names