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P32897

- TIM23_YEAST

UniProt

P32897 - TIM23_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM23

Gene

TIM23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

    GO - Molecular functioni

    1. mitochondrion targeting sequence binding Source: SGD
    2. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
    3. protein binding Source: IntAct
    4. protein channel activity Source: SGD

    GO - Biological processi

    1. protein import into mitochondrial matrix Source: SGD

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33332-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM23
    Alternative name(s):
    Membrane import machinery protein MIM23
    Mitochondrial protein import protein 3
    Mitochondrial protein import protein MAS6
    Gene namesi
    Name:TIM23
    Synonyms:MAS6, MIM23, MPI3
    Ordered Locus Names:YNR017W
    ORF Names:N3180
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR017w.
    SGDiS000005300. TIM23.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Around 20 amino acids of the N-terminus are believed to span the mitochondrial outer membrane. This association is dynamic and depends on the translocation activity of the TIM23 complex. However, this topology seems not to be critical for formation of a TOM-TIM supercomplex and preprotein import.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Reactome
    3. mitochondrial inner membrane presequence translocase complex Source: SGD
    4. mitochondrial intermembrane space Source: Reactome

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Mitochondrial import inner membrane translocase subunit TIM23PRO_0000210307Add
    BLAST

    Proteomic databases

    MaxQBiP32897.
    PaxDbiP32897.
    PeptideAtlasiP32897.

    Expressioni

    Gene expression databases

    GenevestigatoriP32897.

    Interactioni

    Subunit structurei

    Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAM16P429496EBI-9136,EBI-26019
    TIM17P3951513EBI-9136,EBI-9127
    TIM21P5322014EBI-9136,EBI-23128
    TIM44Q018528EBI-9136,EBI-9141
    TIM50Q0277612EBI-9136,EBI-30302

    Protein-protein interaction databases

    BioGridi35842. 50 interactions.
    DIPiDIP-2451N.
    IntActiP32897. 15 interactions.
    MINTiMINT-1359281.
    STRINGi4932.YNR017W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32897.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini? – 96Mitochondrial intermembraneSequence Analysis
    Topological domaini119 – 14426Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini167 – 1748Mitochondrial intermembraneSequence Analysis
    Topological domaini190 – 1967Mitochondrial matrixSequence Analysis
    Topological domaini216 – 2227Mitochondrial intermembraneSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – ?Helical; Note=Outer membraneSequence Analysis
    Transmembranei97 – 11822Helical; Note=Inner membraneSequence AnalysisAdd
    BLAST
    Transmembranei145 – 16622Helical; Note=Inner membraneSequence AnalysisAdd
    BLAST
    Transmembranei175 – 18915Helical; Note=Inner membraneSequence AnalysisAdd
    BLAST
    Transmembranei197 – 21519Helical; Note=Inner membraneSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Tim17/Tim22/Tim23 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5596.
    HOGENOMiHOG000166435.
    KOiK17794.
    OMAiHITKRGP.
    OrthoDBiEOG75MW7H.

    Family and domain databases

    InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
    IPR005681. Tim23.
    [Graphical view]
    PfamiPF02466. Tim17. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00983. 3a0801s02tim23. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32897-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG    50
    MHVDTARLHP LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG 100
    TGAVYLLGLG IGGFSGMMQG LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN 150
    NAGILALSYN IINSTIDALR GKHDTAGSIG AGALTGALFK SSKGLKPMGY 200
    SSAMVAAACA VWCSVKKRLL EK 222
    Length:222
    Mass (Da):23,244
    Last modified:October 1, 1993 - v1
    Checksum:i08E5AB4F400F675B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71633 Genomic DNA. Translation: CAA50640.1.
    X74161 Genomic DNA. Translation: CAA52274.1.
    Z71632 Genomic DNA. Translation: CAA96296.1.
    BK006947 Genomic DNA. Translation: DAA10558.1.
    PIRiS36139.
    RefSeqiNP_014414.3. NM_001183194.3.

    Genome annotation databases

    EnsemblFungiiYNR017W; YNR017W; YNR017W.
    GeneIDi855751.
    KEGGisce:YNR017W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71633 Genomic DNA. Translation: CAA50640.1 .
    X74161 Genomic DNA. Translation: CAA52274.1 .
    Z71632 Genomic DNA. Translation: CAA96296.1 .
    BK006947 Genomic DNA. Translation: DAA10558.1 .
    PIRi S36139.
    RefSeqi NP_014414.3. NM_001183194.3.

    3D structure databases

    ProteinModelPortali P32897.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35842. 50 interactions.
    DIPi DIP-2451N.
    IntActi P32897. 15 interactions.
    MINTi MINT-1359281.
    STRINGi 4932.YNR017W.

    Chemistry

    BindingDBi P32897.
    ChEMBLi CHEMBL1741180.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P32897.
    PaxDbi P32897.
    PeptideAtlasi P32897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR017W ; YNR017W ; YNR017W .
    GeneIDi 855751.
    KEGGi sce:YNR017W.

    Organism-specific databases

    CYGDi YNR017w.
    SGDi S000005300. TIM23.

    Phylogenomic databases

    eggNOGi COG5596.
    HOGENOMi HOG000166435.
    KOi K17794.
    OMAi HITKRGP.
    OrthoDBi EOG75MW7H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33332-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 980166.
    PROi P32897.

    Gene expression databases

    Genevestigatori P32897.

    Family and domain databases

    InterProi IPR003397. Tim17/Tim22/Tim23/PMP24.
    IPR005681. Tim23.
    [Graphical view ]
    Pfami PF02466. Tim17. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00983. 3a0801s02tim23. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway."
      Emtage J.L.T., Jensen R.E.
      J. Cell Biol. 122:1003-1012(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane."
      Dekker P.J.T., Keil P., Rassow J., Maarse A.C., Pfanner N., Meijer M.
      FEBS Lett. 330:66-70(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
      Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
      FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, TOPOLOGY.
    6. Cited for: TOPOLOGY.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
      Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
      Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
    9. "Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria."
      Popov-Celeketic D., Mapa K., Neupert W., Mokranjac D.
      EMBO J. 27:1469-1480(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TOPOLOGY.

    Entry informationi

    Entry nameiTIM23_YEAST
    AccessioniPrimary (citable) accession number: P32897
    Secondary accession number(s): D6W1J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1440 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3