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P32893

- TRS65_YEAST

UniProt

P32893 - TRS65_YEAST

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Protein
Trafficking protein particle complex II-specific subunit 65
Gene
TRS65, KRE11, YGR166W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchanger (GEF) for the Golgi YPT1 GTPase. TRS65 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS120 and TRS130. Involved in cell wall (1-->6)-beta-glucan synthesis.3 Publications

Pathwayi

GO - Biological processi

  1. beta-glucan biosynthetic process Source: UniProtKB-UniPathway
  2. intra-Golgi vesicle-mediated transport Source: SGD
  3. protein complex assembly Source: SGD
  4. regulation of Rab GTPase activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30864-MONOMER.
UniPathwayiUPA00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex II-specific subunit 65
Short name:
TRAPP II-specific subunit 65
Alternative name(s):
Beta-glucan synthesis-associated protein TRS65
Killer toxin-resistance protein 11
Transport protein particle 65 kDa subunit
Gene namesi
Name:TRS65
Synonyms:KRE11
Ordered Locus Names:YGR166W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR166w.
SGDiS000003398. TRS65.

Subcellular locationi

Cytoplasm. Golgi apparatuscis-Golgi network 2 Publications

GO - Cellular componenti

  1. TRAPPII protein complex Source: SGD
  2. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Trafficking protein particle complex II-specific subunit 65
PRO_0000084328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei393 – 3931Phosphoserine2 Publications
Modified residuei398 – 3981Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32893.
PaxDbiP32893.
PeptideAtlasiP32893.

Expressioni

Gene expression databases

GenevestigatoriP32893.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts directly with TRS120 and TRS130.4 Publications

Protein-protein interaction databases

BioGridi33418. 141 interactions.
DIPiDIP-5104N.
IntActiP32893. 8 interactions.
MINTiMINT-528179.
STRINGi4932.YGR166W.

Structurei

3D structure databases

ProteinModelPortaliP32893.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG46088.
OMAiNVVMNNG.
OrthoDBiEOG7JX3G7.

Family and domain databases

InterProiIPR024662. TRAPP_II_complex_Trs65.
[Graphical view]
PfamiPF12735. Trs65. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32893-1 [UniParc]FASTAAdd to Basket

« Hide

MECFVPLRCD LDGSNIEQLR QSHLSRKFII FDEQLNLWLW FQGNSQENKR    50
FVLQNMIILI NEAQVTRTST IDDYFTQVEN NENLWRLKND CCSKILFKSN 100
VVMNNGYNNQ IKFVFEYKSV DANFNNQDSL QDPQAKYTLD KYSSEEILPS 150
FEPVYSWSSA ATKSSKNTNN HLEKNNRATH RVSSKNSEVH EADVSRNPNT 200
FTLKLQYPIF SLLNMRLRNI SLKSEHCILS SLDFQTSKAS EQLTKKFIYP 250
QEHNSFLKLN FQEISYKLID GTSQIELDPI CPLKVPLTAF SYDSISATFK 300
LVLLPKSTQP HRVKITLAYE LELHPNLKLP VRTSWETEVT LKRSMPISST 350
SSQYSSNNNN TNHSASFNGA ANNVNSGGLA NLRLGGVSSS RFSLGAASTT 400
SLVNSKLSNV KFKFINSNIK VIKGEKFTMR LQIINSSSSP LDLVVYYNNT 450
INPIPSANNV RNSNGINNCG MNNGTIPNSP LTLENQYQLH NKYRKIAEGI 500
ILLSNDYKIP VVPPRETYFA DLRFIGIMSG YYGTLSGLKV LDLNTNELIE 550
VGNGASVLIQ 560
Length:560
Mass (Da):63,368
Last modified:October 1, 1993 - v1
Checksum:i9039A2CEBB1F8316
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10667 Genomic DNA. Translation: AAA34727.1.
Z72951 Genomic DNA. Translation: CAA97191.1.
BK006941 Genomic DNA. Translation: DAA08260.1.
PIRiS42158.
RefSeqiNP_011682.1. NM_001181295.1.

Genome annotation databases

EnsemblFungiiYGR166W; YGR166W; YGR166W.
GeneIDi853076.
KEGGisce:YGR166W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10667 Genomic DNA. Translation: AAA34727.1 .
Z72951 Genomic DNA. Translation: CAA97191.1 .
BK006941 Genomic DNA. Translation: DAA08260.1 .
PIRi S42158.
RefSeqi NP_011682.1. NM_001181295.1.

3D structure databases

ProteinModelPortali P32893.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33418. 141 interactions.
DIPi DIP-5104N.
IntActi P32893. 8 interactions.
MINTi MINT-528179.
STRINGi 4932.YGR166W.

Proteomic databases

MaxQBi P32893.
PaxDbi P32893.
PeptideAtlasi P32893.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR166W ; YGR166W ; YGR166W .
GeneIDi 853076.
KEGGi sce:YGR166W.

Organism-specific databases

CYGDi YGR166w.
SGDi S000003398. TRS65.

Phylogenomic databases

eggNOGi NOG46088.
OMAi NVVMNNG.
OrthoDBi EOG7JX3G7.

Enzyme and pathway databases

UniPathwayi UPA00636 .
BioCyci YEAST:G3O-30864-MONOMER.

Miscellaneous databases

NextBioi 973035.

Gene expression databases

Genevestigatori P32893.

Family and domain databases

InterProi IPR024662. TRAPP_II_complex_Trs65.
[Graphical view ]
Pfami PF12735. Trs65. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis."
    Brown J.L., Kossaczka Z., Jiang B., Bussey H.
    Genetics 133:837-849(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
    Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
    EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
  6. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
    Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
    Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
    Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
    Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TRS120 AND TRS130.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-398, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
    Yip C.K., Berscheminski J., Walz T.
    Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.

Entry informationi

Entry nameiTRS65_YEAST
AccessioniPrimary (citable) accession number: P32893
Secondary accession number(s): D6VUU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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