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P32893

- TRS65_YEAST

UniProt

P32893 - TRS65_YEAST

Protein

Trafficking protein particle complex II-specific subunit 65

Gene

TRS65

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchanger (GEF) for the Golgi YPT1 GTPase. TRS65 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS120 and TRS130. Involved in cell wall (1-->6)-beta-glucan synthesis.3 Publications

    Pathwayi

    GO - Biological processi

    1. beta-glucan biosynthetic process Source: UniProtKB-UniPathway
    2. intra-Golgi vesicle-mediated transport Source: SGD
    3. positive regulation of Rab GTPase activity Source: GOC
    4. protein complex assembly Source: SGD

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30864-MONOMER.
    UniPathwayiUPA00636.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking protein particle complex II-specific subunit 65
    Short name:
    TRAPP II-specific subunit 65
    Alternative name(s):
    Beta-glucan synthesis-associated protein TRS65
    Killer toxin-resistance protein 11
    Transport protein particle 65 kDa subunit
    Gene namesi
    Name:TRS65
    Synonyms:KRE11
    Ordered Locus Names:YGR166W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR166w.
    SGDiS000003398. TRS65.

    Subcellular locationi

    GO - Cellular componenti

    1. trans-Golgi network Source: SGD
    2. TRAPPII protein complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Trafficking protein particle complex II-specific subunit 65PRO_0000084328Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei393 – 3931Phosphoserine2 Publications
    Modified residuei398 – 3981Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32893.
    PaxDbiP32893.
    PeptideAtlasiP32893.

    Expressioni

    Gene expression databases

    GenevestigatoriP32893.

    Interactioni

    Subunit structurei

    Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts directly with TRS120 and TRS130.4 Publications

    Protein-protein interaction databases

    BioGridi33418. 141 interactions.
    DIPiDIP-5104N.
    IntActiP32893. 8 interactions.
    MINTiMINT-528179.
    STRINGi4932.YGR166W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32893.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG46088.
    OMAiNVVMNNG.
    OrthoDBiEOG7JX3G7.

    Family and domain databases

    InterProiIPR024662. TRAPP_II_complex_Trs65.
    [Graphical view]
    PfamiPF12735. Trs65. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32893-1 [UniParc]FASTAAdd to Basket

    « Hide

    MECFVPLRCD LDGSNIEQLR QSHLSRKFII FDEQLNLWLW FQGNSQENKR    50
    FVLQNMIILI NEAQVTRTST IDDYFTQVEN NENLWRLKND CCSKILFKSN 100
    VVMNNGYNNQ IKFVFEYKSV DANFNNQDSL QDPQAKYTLD KYSSEEILPS 150
    FEPVYSWSSA ATKSSKNTNN HLEKNNRATH RVSSKNSEVH EADVSRNPNT 200
    FTLKLQYPIF SLLNMRLRNI SLKSEHCILS SLDFQTSKAS EQLTKKFIYP 250
    QEHNSFLKLN FQEISYKLID GTSQIELDPI CPLKVPLTAF SYDSISATFK 300
    LVLLPKSTQP HRVKITLAYE LELHPNLKLP VRTSWETEVT LKRSMPISST 350
    SSQYSSNNNN TNHSASFNGA ANNVNSGGLA NLRLGGVSSS RFSLGAASTT 400
    SLVNSKLSNV KFKFINSNIK VIKGEKFTMR LQIINSSSSP LDLVVYYNNT 450
    INPIPSANNV RNSNGINNCG MNNGTIPNSP LTLENQYQLH NKYRKIAEGI 500
    ILLSNDYKIP VVPPRETYFA DLRFIGIMSG YYGTLSGLKV LDLNTNELIE 550
    VGNGASVLIQ 560
    Length:560
    Mass (Da):63,368
    Last modified:October 1, 1993 - v1
    Checksum:i9039A2CEBB1F8316
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10667 Genomic DNA. Translation: AAA34727.1.
    Z72951 Genomic DNA. Translation: CAA97191.1.
    BK006941 Genomic DNA. Translation: DAA08260.1.
    PIRiS42158.
    RefSeqiNP_011682.1. NM_001181295.1.

    Genome annotation databases

    EnsemblFungiiYGR166W; YGR166W; YGR166W.
    GeneIDi853076.
    KEGGisce:YGR166W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10667 Genomic DNA. Translation: AAA34727.1 .
    Z72951 Genomic DNA. Translation: CAA97191.1 .
    BK006941 Genomic DNA. Translation: DAA08260.1 .
    PIRi S42158.
    RefSeqi NP_011682.1. NM_001181295.1.

    3D structure databases

    ProteinModelPortali P32893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33418. 141 interactions.
    DIPi DIP-5104N.
    IntActi P32893. 8 interactions.
    MINTi MINT-528179.
    STRINGi 4932.YGR166W.

    Proteomic databases

    MaxQBi P32893.
    PaxDbi P32893.
    PeptideAtlasi P32893.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR166W ; YGR166W ; YGR166W .
    GeneIDi 853076.
    KEGGi sce:YGR166W.

    Organism-specific databases

    CYGDi YGR166w.
    SGDi S000003398. TRS65.

    Phylogenomic databases

    eggNOGi NOG46088.
    OMAi NVVMNNG.
    OrthoDBi EOG7JX3G7.

    Enzyme and pathway databases

    UniPathwayi UPA00636 .
    BioCyci YEAST:G3O-30864-MONOMER.

    Miscellaneous databases

    NextBioi 973035.

    Gene expression databases

    Genevestigatori P32893.

    Family and domain databases

    InterProi IPR024662. TRAPP_II_complex_Trs65.
    [Graphical view ]
    Pfami PF12735. Trs65. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis."
      Brown J.L., Kossaczka Z., Jiang B., Bussey H.
      Genetics 133:837-849(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
      Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
      EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
    6. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
      Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
      Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
      Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
      Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TRS120 AND TRS130.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-398, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
      Yip C.K., Berscheminski J., Walz T.
      Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.

    Entry informationi

    Entry nameiTRS65_YEAST
    AccessioniPrimary (citable) accession number: P32893
    Secondary accession number(s): D6VUU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1660 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3