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P32893 (TRS65_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex II-specific subunit 65

Short name=TRAPP II-specific subunit 65
Alternative name(s):
Beta-glucan synthesis-associated protein TRS65
Killer toxin-resistance protein 11
Transport protein particle 65 kDa subunit
Gene names
Name:TRS65
Synonyms:KRE11
Ordered Locus Names:YGR166W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchanger (GEF) for the Golgi YPT1 GTPase. TRS65 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS120 and TRS130. Involved in cell wall (1-->6)-beta-glucan synthesis. Ref.6 Ref.10 Ref.13

Pathway

Glycan metabolism; beta-glucan biosynthesis.

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts directly with TRS120 and TRS130. Ref.5 Ref.6 Ref.10 Ref.13

Subcellular location

Cytoplasm. Golgi apparatuscis-Golgi network Ref.6 Ref.10.

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Trafficking protein particle complex II-specific subunit 65
PRO_0000084328

Amino acid modifications

Modified residue3931Phosphoserine Ref.11 Ref.12
Modified residue3981Phosphoserine Ref.12

Sequences

Sequence LengthMass (Da)Tools
P32893 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 9039A2CEBB1F8316

FASTA56063,368
        10         20         30         40         50         60 
MECFVPLRCD LDGSNIEQLR QSHLSRKFII FDEQLNLWLW FQGNSQENKR FVLQNMIILI 

        70         80         90        100        110        120 
NEAQVTRTST IDDYFTQVEN NENLWRLKND CCSKILFKSN VVMNNGYNNQ IKFVFEYKSV 

       130        140        150        160        170        180 
DANFNNQDSL QDPQAKYTLD KYSSEEILPS FEPVYSWSSA ATKSSKNTNN HLEKNNRATH 

       190        200        210        220        230        240 
RVSSKNSEVH EADVSRNPNT FTLKLQYPIF SLLNMRLRNI SLKSEHCILS SLDFQTSKAS 

       250        260        270        280        290        300 
EQLTKKFIYP QEHNSFLKLN FQEISYKLID GTSQIELDPI CPLKVPLTAF SYDSISATFK 

       310        320        330        340        350        360 
LVLLPKSTQP HRVKITLAYE LELHPNLKLP VRTSWETEVT LKRSMPISST SSQYSSNNNN 

       370        380        390        400        410        420 
TNHSASFNGA ANNVNSGGLA NLRLGGVSSS RFSLGAASTT SLVNSKLSNV KFKFINSNIK 

       430        440        450        460        470        480 
VIKGEKFTMR LQIINSSSSP LDLVVYYNNT INPIPSANNV RNSNGINNCG MNNGTIPNSP 

       490        500        510        520        530        540 
LTLENQYQLH NKYRKIAEGI ILLSNDYKIP VVPPRETYFA DLRFIGIMSG YYGTLSGLKV 

       550        560 
LDLNTNELIE VGNGASVLIQ 

« Hide

References

« Hide 'large scale' references
[1]"A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis."
Brown J.L., Kossaczka Z., Jiang B., Bussey H.
Genetics 133:837-849(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[6]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TRS120 AND TRS130.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-398, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10667 Genomic DNA. Translation: AAA34727.1.
Z72951 Genomic DNA. Translation: CAA97191.1.
BK006941 Genomic DNA. Translation: DAA08260.1.
PIRS42158.
RefSeqNP_011682.1. NM_001181295.1.

3D structure databases

ProteinModelPortalP32893.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33418. 141 interactions.
DIPDIP-5104N.
IntActP32893. 8 interactions.
MINTMINT-528179.
STRING4932.YGR166W.

Proteomic databases

MaxQBP32893.
PaxDbP32893.
PeptideAtlasP32893.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR166W; YGR166W; YGR166W.
GeneID853076.
KEGGsce:YGR166W.

Organism-specific databases

CYGDYGR166w.
SGDS000003398. TRS65.

Phylogenomic databases

eggNOGNOG46088.
OMANVVMNNG.
OrthoDBEOG7JX3G7.

Enzyme and pathway databases

BioCycYEAST:G3O-30864-MONOMER.
UniPathwayUPA00636.

Gene expression databases

GenevestigatorP32893.

Family and domain databases

InterProIPR024662. TRAPP_II_complex_Trs65.
[Graphical view]
PfamPF12735. Trs65. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973035.

Entry information

Entry nameTRS65_YEAST
AccessionPrimary (citable) accession number: P32893
Secondary accession number(s): D6VUU9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PATHWAY comments

Index of metabolic and biosynthesis pathways