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Protein

ATP-dependent RNA helicase DRS1

Gene

DRS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in ribosome assembly.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi275 – 2828ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • ribosomal large subunit assembly Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32113-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DRS1 (EC:3.6.4.13)
Alternative name(s):
Deficiency of ribosomal subunits protein 1
Gene namesi
Name:DRS1
Ordered Locus Names:YLL008W
ORF Names:L1345
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL008W.
SGDiS000003931. DRS1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: UniProtKB-SubCell
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi191 – 1911M → T: No growth at 13 degrees Celsius; when associated with Q-431 and G-472. 1 Publication
Mutagenesisi260 – 2601A → V: No growth at 13 degrees Celsius; when associated with P-564. 1 Publication
Mutagenesisi273 – 2731A → T: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi288 – 2881P → L: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi291 – 2911E → V: No growth at 13 degrees Celsius; when associated with G-637. 1 Publication
Mutagenesisi305 – 3051V → D: No growth at 13 degrees Celsius; when associated with V-306. 1 Publication
Mutagenesisi306 – 3061I → V: No growth at 13 degrees Celsius; when associated with D-305. 1 Publication
Mutagenesisi414 – 4141L → S: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi429 – 4291L → P: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi431 – 4311L → Q: No growth at 13 degrees Celsius; when associated with T-191 and G-472. 1 Publication
Mutagenesisi472 – 4721R → G: No growth at 13 degrees Celsius; when associated with T-191 and Q-431. 1 Publication
Mutagenesisi509 – 5091L → S: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi563 – 5631Y → C: No growth at 13 degrees Celsius. 1 Publication
Mutagenesisi564 – 5641L → P: No growth at 13 degrees Celsius; when associated with V-260. 1 Publication
Mutagenesisi637 – 6371D → G: No growth at 13 degrees Celsius; when associated with V-291. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752ATP-dependent RNA helicase DRS1PRO_0000055045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei208 – 2081PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32892.

PTM databases

iPTMnetiP32892.

Interactioni

Subunit structurei

Interacts with RRP1 and associates with pre-ribosomal particles.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DBP10Q123894EBI-6170,EBI-5644
ERB1Q046604EBI-6170,EBI-28098
LOC1P435864EBI-6170,EBI-22906
NOP4P378385EBI-6170,EBI-12122
RLP7P406933EBI-6170,EBI-15415

Protein-protein interaction databases

BioGridi31243. 82 interactions.
DIPiDIP-6471N.
IntActiP32892. 27 interactions.
MINTiMINT-648259.

Structurei

3D structure databases

ProteinModelPortaliP32892.
SMRiP32892. Positions 233-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 437176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini448 – 639192Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili621 – 66747Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi231 – 25929Q motifAdd
BLAST
Motifi385 – 3884DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi170 – 19021Poly-GluAdd
BLAST

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074997.
HOGENOMiHOG000265456.
InParanoidiP32892.
KOiK13181.
OMAiDEELAMD.
OrthoDBiEOG092C1VLP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVGTKKYSN LDFVPTISDS EDDVPILDSS DDEKVEAKKT TKKRKGKNNK
60 70 80 90 100
KKVSEGDNLD EDVHEDLDAG FKFDLDADDT TSNFQGWNFL AEGESNKDDA
110 120 130 140 150
EAFVKKDVDL DKIIRRKGGL VKMAHIDSKQ EEETEKEKVE KENDSDDEEL
160 170 180 190 200
AMDGFGMGAP MNNGDENQSE EEEEEEEKEE EEEEEEEQEE MTLEKGGKDD
210 220 230 240 250
EIDEEDDSEE AKADFYAPET EGDEAKKQMY ENFNSLSLSR PVLKGLASLG
260 270 280 290 300
YVKPSPIQSA TIPIALLGKD IIAGAVTGSG KTAAFMIPII ERLLYKPAKI
310 320 330 340 350
ASTRVIVLLP TRELAIQVAD VGKQIARFVS GITFGLAVGG LNLRQQEQML
360 370 380 390 400
KSRPDIVIAT PGRFIDHIRN SASFNVDSVE ILVMDEADRM LEEGFQDELN
410 420 430 440 450
EIMGLLPSNR QNLLFSATMN SKIKSLVSLS LKKPVRIMID PPKKAATKLT
460 470 480 490 500
QEFVRIRKRD HLKPALLFNL IRKLDPTGQK RIVVFVARKE TAHRLRIIMG
510 520 530 540 550
LLGMSVGELH GSLTQEQRLD SVNKFKNLEV PVLICTDLAS RGLDIPKIEV
560 570 580 590 600
VINYDMPKSY EIYLHRVGRT ARAGREGRSV TFVGESSQDR SIVRAAIKSV
610 620 630 640 650
EENKSLTQGK ALGRNVDWVQ IEETNKLVES MNDTIEDILV EEKEEKEILR
660 670 680 690 700
AEMQLRKGEN MLKHKKEIQA RPRRTWFQSE SDKKNSKVLG ALSRNKKVTN
710 720 730 740 750
SKKRKREEAK ADGNGARSYR KTKTDRIADQ ERTFKKQKST NSNKKKGFKS

RR
Length:752
Mass (Da):84,843
Last modified:October 1, 1996 - v2
Checksum:i60747607A6E5E4A8
GO

Sequence cautioni

The sequence AAA34666 differs from that shown. Reason: Frameshift at positions 12 and 69. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00683 Genomic DNA. Translation: AAA34666.1. Frameshift.
X91488 Genomic DNA. Translation: CAA62783.1.
Z73113 Genomic DNA. Translation: CAA97452.1.
BK006945 Genomic DNA. Translation: DAA09310.1.
PIRiS64750.
RefSeqiNP_013093.1. NM_001181828.1.

Genome annotation databases

EnsemblFungiiYLL008W; YLL008W; YLL008W.
GeneIDi850652.
KEGGisce:YLL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00683 Genomic DNA. Translation: AAA34666.1. Frameshift.
X91488 Genomic DNA. Translation: CAA62783.1.
Z73113 Genomic DNA. Translation: CAA97452.1.
BK006945 Genomic DNA. Translation: DAA09310.1.
PIRiS64750.
RefSeqiNP_013093.1. NM_001181828.1.

3D structure databases

ProteinModelPortaliP32892.
SMRiP32892. Positions 233-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31243. 82 interactions.
DIPiDIP-6471N.
IntActiP32892. 27 interactions.
MINTiMINT-648259.

PTM databases

iPTMnetiP32892.

Proteomic databases

MaxQBiP32892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL008W; YLL008W; YLL008W.
GeneIDi850652.
KEGGisce:YLL008W.

Organism-specific databases

EuPathDBiFungiDB:YLL008W.
SGDiS000003931. DRS1.

Phylogenomic databases

GeneTreeiENSGT00550000074997.
HOGENOMiHOG000265456.
InParanoidiP32892.
KOiK13181.
OMAiDEELAMD.
OrthoDBiEOG092C1VLP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32113-MONOMER.

Miscellaneous databases

PROiP32892.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRS1_YEAST
AccessioniPrimary (citable) accession number: P32892
Secondary accession number(s): D6VXZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.