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Protein

D-lactate dehydrogenase [cytochrome] 1, mitochondrial

Gene

DLD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific oxidation of D-lactate to pyruvate.

Miscellaneous

Present with 10800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

Cofactori

Protein has several cofactor binding sites:
  • FADNote: Binds 2 FAD.
  • Zn2+Note: Binds 4 to 6 Zn2+ ions.

GO - Molecular functioni

  • D-lactate dehydrogenase (cytochrome) activity Source: SGD
  • flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  • lactate catabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, Zinc

Enzyme and pathway databases

BioCyciYEAST:YDL174C-MONOMER
ReactomeiR-SCE-9033241 Peroxisomal protein import

Names & Taxonomyi

Protein namesi
Recommended name:
D-lactate dehydrogenase [cytochrome] 1, mitochondrial (EC:1.1.2.4)
Alternative name(s):
D-lactate ferricytochrome C oxidoreductase
Short name:
D-LCR
Gene namesi
Name:DLD1
Synonyms:DLD
Ordered Locus Names:YDL174C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL174C
SGDiS000002333 DLD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020428? – 587D-lactate dehydrogenase [cytochrome] 1, mitochondrial
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiP32891
PaxDbiP32891
PRIDEiP32891

2D gel databases

UCD-2DPAGEiP32891

Expressioni

Inductioni

By D-lactate. Induced during respiratory adaptation.

Interactioni

Protein-protein interaction databases

BioGridi31892, 73 interactors
IntActiP32891, 15 interactors
STRINGi4932.YDL174C

Structurei

3D structure databases

ProteinModelPortaliP32891
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 327FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST182

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063515
HOGENOMiHOG000230995
InParanoidiP32891
KOiK00102
OMAiTPRTCGE
OrthoDBiEOG092C1K74

Family and domain databases

Gene3Di1.10.45.10, 1 hit
InterProiView protein in InterPro
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR016164 FAD-linked_Oxase-like_C
IPR004113 FAD-linked_oxidase_C
IPR006094 Oxid_FAD_bind_N
IPR016171 Vanillyl_alc_oxidase_C-sub2
PfamiView protein in Pfam
PF02913 FAD-oxidase_C, 1 hit
PF01565 FAD_binding_4, 1 hit
SUPFAMiSSF55103 SSF55103, 1 hit
SSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWKRTCTRL IKPIAQPRGR LVRRSCYRYA STGTGSTDSS SQWLKYSVIA
60 70 80 90 100
SSATLFGYLF AKNLYSRETK EDLIEKLEMV KKIDPVNSTL KLSSLDSPDY
110 120 130 140 150
LHDPVKIDKV VEDLKQVLGN KPENYSDAKS DLDAHSDTYF NTHHPSPEQR
160 170 180 190 200
PRIILFPHTT EEVSKILKIC HDNNMPVVPF SGGTSLEGHF LPTRIGDTIT
210 220 230 240 250
VDLSKFMNNV VKFDKLDLDI TVQAGLPWED LNDYLSDHGL MFGCDPGPGA
260 270 280 290 300
QIGGCIANSC SGTNAYRYGT MKENIINMTI VLPDGTIVKT KKRPRKSSAG
310 320 330 340 350
YNLNGLFVGS EGTLGIVTEA TVKCHVKPKA ETVAVVSFDT IKDAAACASN
360 370 380 390 400
LTQSGIHLNA MELLDENMMK LINASESTDR CDWVEKPTMF FKIGGRSPNI
410 420 430 440 450
VNALVDEVKA VAQLNHCNSF QFAKDDDEKL ELWEARKVAL WSVLDADKSK
460 470 480 490 500
DKSAKIWTTD VAVPVSQFDK VIHETKKDMQ ASKLINAIVG HAGDGNFHAF
510 520 530 540 550
IVYRTPEEHE TCSQLVDRMV KRALNAEGTC TGEHGVGIGK REYLLEELGE
560 570 580
APVDLMRKIK LAIDPKRIMN PDKIFKTDPN EPANDYR
Length:587
Mass (Da):65,293
Last modified:November 1, 1997 - v2
Checksum:i07183BEAEEB2EB19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti439A → RR in CAA46852 (PubMed:8492799).Curated1
Sequence conflicti572 – 587DKIFK…ANDYR → GQNL in CAA46852 (PubMed:8492799).CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66052 Genomic DNA Translation: CAA46852.1
Z67750 Genomic DNA Translation: CAA91571.1
Z74222 Genomic DNA Translation: CAA98748.1
BK006938 Genomic DNA Translation: DAA11688.1
PIRiS61038
RefSeqiNP_010107.1, NM_001180234.1

Genome annotation databases

EnsemblFungiiYDL174C; YDL174C; YDL174C
GeneIDi851380
KEGGisce:YDL174C

Similar proteinsi

Entry informationi

Entry nameiDLD1_YEAST
AccessioniPrimary (citable) accession number: P32891
Secondary accession number(s): D6VRH8, Q12360
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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