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P32884 (HN_NDVB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18
Gene names
Name:HN
OrganismNewcastle disease virus (strain Beaudette C/45) (NDV)
Taxonomic identifier11178 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Avulavirus ›
Virus hostGallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.

Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Hemagglutinin-neuraminidase
PRO_0000142607

Regions

Topological domain1 – 2626Intravirion Potential
Transmembrane27 – 4822Helical; Potential
Topological domain49 – 577529Virion surface Potential

Amino acid modifications

Modified residue11Blocked amino end (Met); by host
Glycosylation1191N-linked (GlcNAc...); by host Potential
Glycosylation3411N-linked (GlcNAc...); by host Potential
Glycosylation4331N-linked (GlcNAc...); by host Potential
Glycosylation4811N-linked (GlcNAc...); by host Potential
Glycosylation5381N-linked (GlcNAc...); by host Potential

Secondary structure

1 ................................................................................... 577
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32884 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 1D4C52B4887EC1A1

FASTA57763,142
        10         20         30         40         50         60 
MDRAVSQVAL ENDEREAKNT WRLIFRIAIL LLTVVTLATS VASLVYSMGA STPSDLVGIP 

        70         80         90        100        110        120 
TRISRAEEKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE TTIMNAITSL SYQINGAANN 

       130        140        150        160        170        180 
SGWGAPIHDP DFIGGIGKEL IVDDASDVTS FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM 

       190        200        210        220        230        240 
SATHYCYTHN VILSGCRDHS HSHQYLALGV LRTTATGRIF FSTLRSISLD DTQNRKSCSV 

       250        260        270        280        290        300 
SATPLGCDML CSKVTETEEE DYNSAVPTLM AHGRLGFDGQ YHEKDLDVTT LFEDWVANYP 

       310        320        330        340        350        360 
GVGGGSFIDG RVWFSVYGGL KPNSPSDTVQ EGKYVIYKRY NDTCPDEQDY QIRMAKSSYK 

       370        380        390        400        410        420 
PGRFGGKRIQ QAILSIKVST SLGEDPVLTV PPNTVTLMGA EGRILTVGTS HFLYQRGSSY 

       430        440        450        460        470        480 
FSPALLYPMT VSNKTATLHS PYTFNAFTRP GSIPCQASAR CPNSCVTGVY TDPYPLIFYR 

       490        500        510        520        530        540 
NHTLRGVFGT MLDSEQARLN PTSAVFDSTS RSRITRVSSS STKAAYTTST CFKVVKTNKT 

       550        560        570 
YCLSIAEISN TLFGEFRIVP LLVEILKNDG VREARSG

« Hide

References

[1]"Nucleotide sequence analysis of the haemagglutinin-neuraminidase gene of Newcastle disease virus."
Millar N.S., Chambers P., Emmerson P.T.
J. Gen. Virol. 67:1917-1927(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X04355 Genomic RNA. Translation: CAA27880.1.
M24710 Genomic RNA. Translation: AAA46660.1.
PIRF46328.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1USRX-ray2.00A/B124-577[»]
1USXX-ray2.70A/B/C124-577[»]
ProteinModelPortalP32884.
SMRP32884. Positions 124-572.
ModBaseSearch...

Protein family/group databases

CAZyGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamPF00423. HN. 1 hit.
[Graphical view]
PIRSFPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMSSF50939. Sialidase. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP32884.

Entry information

Entry nameHN_NDVB
AccessionPrimary (citable) accession number: P32884
Secondary accession number(s): P06158
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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