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P32883

- RASK_MOUSE

UniProt

P32883 - RASK_MOUSE

Protein

GTPase KRas

Gene

Kras

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTP
    Nucleotide bindingi57 – 615GTP
    Nucleotide bindingi116 – 1194GTP

    GO - Molecular functioni

    1. GDP binding Source: Ensembl
    2. GMP binding Source: Ensembl
    3. GTPase activity Source: MGI
    4. GTP binding Source: UniProtKB-KW
    5. protein binding Source: IntAct
    6. protein complex binding Source: MGI

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. cytokine-mediated signaling pathway Source: Ensembl
    3. epithelial tube branching involved in lung morphogenesis Source: MGI
    4. negative regulation of cell differentiation Source: MGI
    5. negative regulation of neuron apoptotic process Source: MGI
    6. positive regulation of cell proliferation Source: MGI
    7. positive regulation of gene expression Source: Ensembl
    8. positive regulation of MAP kinase activity Source: Ensembl
    9. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
    10. positive regulation of nitric-oxide synthase activity Source: Ensembl
    11. positive regulation of Rac protein signal transduction Source: MGI
    12. Ras protein signal transduction Source: MGI
    13. regulation of long-term neuronal synaptic plasticity Source: MGI
    14. regulation of synaptic transmission, GABAergic Source: MGI
    15. response to glucocorticoid Source: Ensembl
    16. response to mineralocorticoid Source: Ensembl
    17. social behavior Source: Ensembl
    18. striated muscle cell differentiation Source: MGI
    19. visual learning Source: MGI

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase KRas
    Alternative name(s):
    K-Ras 2
    Ki-Ras
    c-K-ras
    c-Ki-ras
    Cleaved into the following chain:
    Gene namesi
    Name:Kras
    Synonyms:Kras2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:96680. Kras.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: MGI
    2. membrane raft Source: Ensembl
    3. mitochondrion Source: Ensembl
    4. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi12 – 121G → D: Activates oncogene.
    Mutagenesisi164 – 1641R → A: Loss of GTP-binding activity.

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 186186GTPase KRasPRO_0000082643Add
    BLAST
    Initiator methioninei1 – 11Removed; alternateBy similarity
    Chaini2 – 186185GTPase KRas, N-terminally processedPRO_0000326482Add
    BLAST
    Propeptidei187 – 1893Removed in mature formBy similarityPRO_0000281293

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei2 – 21N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity
    Modified residuei104 – 1041N6-acetyllysineBy similarity
    Lipidationi180 – 1801S-palmitoyl cysteineBy similarity
    Modified residuei186 – 1861Cysteine methyl ester; in isoform 2ABy similarity
    Lipidationi186 – 1861S-farnesyl cysteine; in isoform 2ABy similarity

    Post-translational modificationi

    Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    MaxQBiP32883.
    PaxDbiP32883.
    PRIDEiP32883.

    PTM databases

    PhosphoSiteiP32883.

    Expressioni

    Gene expression databases

    ArrayExpressiP32883.
    BgeeiP32883.
    GenevestigatoriP32883.

    Interactioni

    Subunit structurei

    Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EgfrQ012793EBI-644267,EBI-6296235
    Raf1Q99N573EBI-644285,EBI-397757

    Protein-protein interaction databases

    BioGridi201012. 1 interaction.
    DIPiDIP-29362N.
    IntActiP32883. 7 interactions.
    MINTiMINT-216577.

    Structurei

    3D structure databases

    ProteinModelPortaliP32883.
    SMRiP32883. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 18520Hypervariable regionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector region

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233973.
    HOVERGENiHBG009351.
    InParanoidiP32883.
    KOiK07827.
    OMAiKKSRTRC.
    OrthoDBiEOG7QVM41.
    PhylomeDBiP32883.
    TreeFamiTF312796.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Isoforms differ in the C-terminal region which is encoded by two alternative exons (IVA and IVB).

    Isoform 2A (identifier: P32883-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET    50
    CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI 100
    KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ 150
    RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM 189
    Length:189
    Mass (Da):21,656
    Last modified:August 13, 1987 - v1
    Checksum:i97345422E01D2C81
    GO
    Isoform 2B (identifier: P32883-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         151-153: RVE → GVD
         165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCTVM

    Show »
    Length:188
    Mass (Da):21,483
    Checksum:iB0A542E8AD158F51
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei151 – 1533RVE → GVD in isoform 2B. 2 PublicationsVSP_011142
    Alternative sequencei165 – 18925QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CTVM in isoform 2B. 2 PublicationsVSP_011143Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02452
    , X02453, X02454, X02456 Genomic DNA. Translation: CAA26295.1.
    X02455 Genomic DNA. Translation: CAA26296.1.
    BC004642 mRNA. Translation: AAH04642.1.
    BC010202 mRNA. Translation: AAH10202.1.
    K01927 Genomic DNA. Translation: AAA40037.1.
    X00485 mRNA. Translation: CAA25160.1.
    CCDSiCCDS20693.1. [P32883-2]
    PIRiA01365. TVMSK.
    B01365. TVMS2K.
    RefSeqiNP_067259.4. NM_021284.6. [P32883-2]
    XP_006506981.1. XM_006506918.1. [P32883-1]
    XP_006536102.1. XM_006536039.1. [P32883-1]
    UniGeneiMm.383182.

    Genome annotation databases

    EnsembliENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265. [P32883-2]
    ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265. [P32883-1]
    GeneIDi16653.
    KEGGimmu:16653.
    UCSCiuc009eri.2. mouse. [P32883-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02452
    , X02453 , X02454 , X02456 Genomic DNA. Translation: CAA26295.1 .
    X02455 Genomic DNA. Translation: CAA26296.1 .
    BC004642 mRNA. Translation: AAH04642.1 .
    BC010202 mRNA. Translation: AAH10202.1 .
    K01927 Genomic DNA. Translation: AAA40037.1 .
    X00485 mRNA. Translation: CAA25160.1 .
    CCDSi CCDS20693.1. [P32883-2 ]
    PIRi A01365. TVMSK.
    B01365. TVMS2K.
    RefSeqi NP_067259.4. NM_021284.6. [P32883-2 ]
    XP_006506981.1. XM_006506918.1. [P32883-1 ]
    XP_006536102.1. XM_006536039.1. [P32883-1 ]
    UniGenei Mm.383182.

    3D structure databases

    ProteinModelPortali P32883.
    SMRi P32883. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201012. 1 interaction.
    DIPi DIP-29362N.
    IntActi P32883. 7 interactions.
    MINTi MINT-216577.

    PTM databases

    PhosphoSitei P32883.

    Proteomic databases

    MaxQBi P32883.
    PaxDbi P32883.
    PRIDEi P32883.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032399 ; ENSMUSP00000032399 ; ENSMUSG00000030265 . [P32883-2 ]
    ENSMUST00000111710 ; ENSMUSP00000107339 ; ENSMUSG00000030265 . [P32883-1 ]
    GeneIDi 16653.
    KEGGi mmu:16653.
    UCSCi uc009eri.2. mouse. [P32883-1 ]

    Organism-specific databases

    CTDi 3845.
    MGIi MGI:96680. Kras.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233973.
    HOVERGENi HBG009351.
    InParanoidi P32883.
    KOi K07827.
    OMAi KKSRTRC.
    OrthoDBi EOG7QVM41.
    PhylomeDBi P32883.
    TreeFami TF312796.

    Miscellaneous databases

    ChiTaRSi KRAS. mouse.
    NextBioi 290345.
    PROi P32883.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P32883.
    Bgeei P32883.
    Genevestigatori P32883.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells."
      George D.L., Scott A.F., Trusko S., Glick B., Ford E., Dorney D.J.
      EMBO J. 4:1199-1203(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 2A AND 2B).
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2B).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "Activation of a c-K-ras oncogene by somatic mutation in mouse lymphomas induced by gamma radiation."
      Guerrero I., Villasante A., Corces V., Pellicer A.
      Science 225:1159-1162(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-37.
    4. "Parental bias of Ki-ras oncogenes detected in lung tumors from mouse hybrids."
      You M., Wang Y., Stoner G., You L., Maronpot R., Reynolds S.H., Anderson M.
      Proc. Natl. Acad. Sci. U.S.A. 89:5804-5808(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-37.
    5. "Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6."
      George D.L., Scott A.F., de Martinville B., Francke U.
      Nucleic Acids Res. 12:2731-2743(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-173 (ISOFORM 2B).

    Entry informationi

    Entry nameiRASK_MOUSE
    AccessioniPrimary (citable) accession number: P32883
    Secondary accession number(s): P04200, P08643
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This gene is amplified in the mouse adrenal tumor Y1 cells, and is also directly linked to lung tumor susceptibility.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3