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P32883 (RASK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase KRas
Alternative name(s):
K-Ras 2
Ki-Ras
c-K-ras
c-Ki-ras

Cleaved into the following chain:

  1. GTPase KRas, N-terminally processed
Gene names
Name:Kras
Synonyms:Kras2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Subunit structure

Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14 By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Post-translational modification

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs) By similarity.

Miscellaneous

This gene is amplified in the mouse adrenal tumor Y1 cells, and is also directly linked to lung tumor susceptibility.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DiseaseProto-oncogene
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Lipoprotein
Methylation
Palmitate
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRas protein signal transduction

Inferred from genetic interaction PubMed 15665300. Source: MGI

actin cytoskeleton organization

Inferred from genetic interaction PubMed 15665300. Source: MGI

negative regulation of neuron apoptotic process

Inferred from genetic interaction PubMed 10845775. Source: MGI

positive regulation of Rac protein signal transduction

Inferred from genetic interaction PubMed 15665300. Source: MGI

positive regulation of cell proliferation

Inferred from genetic interaction PubMed 15665300. Source: MGI

regulation of long-term neuronal synaptic plasticity

Inferred from genetic interaction PubMed 11793011. Source: MGI

regulation of synaptic transmission, GABAergic

Inferred from genetic interaction PubMed 11793011. Source: MGI

striated muscle cell differentiation

Inferred from genetic interaction PubMed 15542848. Source: MGI

visual learning

Inferred from genetic interaction PubMed 11793011. Source: MGI

   Cellular_componentmembrane

Inferred from direct assay PubMed 10085069. Source: MGI

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from genetic interaction PubMed 15665300. Source: MGI

protein complex binding

Inferred from sequence orthology PubMed 23209302. Source: MGI

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms differ in the C-terminal region which is encoded by two alternative exons (IVA and IVB).
Isoform 2A (identifier: P32883-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2B (identifier: P32883-2)

The sequence of this isoform differs from the canonical sequence as follows:
     151-153: RVE → GVD
     165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCTVM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 186186GTPase KRas
PRO_0000082643
Initiator methionine11Removed; alternate By similarity
Chain2 – 186185GTPase KRas, N-terminally processed
PRO_0000326482
Propeptide187 – 1893Removed in mature form By similarity
PRO_0000281293

Regions

Nucleotide binding10 – 178GTP
Nucleotide binding57 – 615GTP
Nucleotide binding116 – 1194GTP
Region166 – 18520Hypervariable region
Motif32 – 409Effector region

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue21N-acetylthreonine; in GTPase KRas, N-terminally processed By similarity
Modified residue1041N6-acetyllysine By similarity
Modified residue1861Cysteine methyl ester; in isoform 2A By similarity
Lipidation1801S-palmitoyl cysteine By similarity
Lipidation1861S-farnesyl cysteine; in isoform 2A By similarity

Natural variations

Alternative sequence151 – 1533RVE → GVD in isoform 2B.
VSP_011142
Alternative sequence165 – 18925QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CTVM in isoform 2B.
VSP_011143

Experimental info

Mutagenesis121G → D: Activates oncogene.
Mutagenesis1641R → A: Loss of GTP-binding activity.

Sequences

Sequence LengthMass (Da)Tools
Isoform 2A [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 97345422E01D2C81

FASTA18921,656
        10         20         30         40         50         60 
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 

        70         80         90        100        110        120 
QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL 

       130        140        150        160        170        180 
PSRTVDTKQA QELARSYGIP FIETSAKTRQ RVEDAFYTLV REIRQYRLKK ISKEEKTPGC 


VKIKKCVIM 

« Hide

Isoform 2B [UniParc] [UniParc].

Checksum: B0A542E8AD158F51
Show »

FASTA18821,483

References

« Hide 'large scale' references
[1]"Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells."
George D.L., Scott A.F., Trusko S., Glick B., Ford E., Dorney D.J.
EMBO J. 4:1199-1203(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 2A AND 2B).
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2B).
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"Activation of a c-K-ras oncogene by somatic mutation in mouse lymphomas induced by gamma radiation."
Guerrero I., Villasante A., Corces V., Pellicer A.
Science 225:1159-1162(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-37.
[4]"Parental bias of Ki-ras oncogenes detected in lung tumors from mouse hybrids."
You M., Wang Y., Stoner G., You L., Maronpot R., Reynolds S.H., Anderson M.
Proc. Natl. Acad. Sci. U.S.A. 89:5804-5808(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-37.
[5]"Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6."
George D.L., Scott A.F., de Martinville B., Francke U.
Nucleic Acids Res. 12:2731-2743(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-173 (ISOFORM 2B).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02452 expand/collapse EMBL AC list , X02453, X02454, X02456 Genomic DNA. Translation: CAA26295.1.
X02455 Genomic DNA. Translation: CAA26296.1.
BC004642 mRNA. Translation: AAH04642.1.
BC010202 mRNA. Translation: AAH10202.1.
K01927 Genomic DNA. Translation: AAA40037.1.
X00485 mRNA. Translation: CAA25160.1.
PIRTVMSK. A01365.
TVMS2K. B01365.
RefSeqNP_067259.4. NM_021284.6.
XP_006506981.1. XM_006506918.1.
XP_006536102.1. XM_006536039.1.
UniGeneMm.383182.

3D structure databases

ProteinModelPortalP32883.
SMRP32883. Positions 1-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201012. 1 interaction.
DIPDIP-29362N.
IntActP32883. 7 interactions.
MINTMINT-216577.

PTM databases

PhosphoSiteP32883.

Proteomic databases

PaxDbP32883.
PRIDEP32883.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265. [P32883-2]
ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265. [P32883-1]
GeneID16653.
KEGGmmu:16653.
UCSCuc009eri.2. mouse. [P32883-1]

Organism-specific databases

CTD3845.
MGIMGI:96680. Kras.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233973.
HOVERGENHBG009351.
InParanoidP32883.
KOK07827.
OMAKKSRTRC.
OrthoDBEOG7QVM41.
PhylomeDBP32883.
TreeFamTF312796.

Gene expression databases

ArrayExpressP32883.
BgeeP32883.
GenevestigatorP32883.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKRAS. mouse.
NextBio290345.
PROP32883.
SOURCESearch...

Entry information

Entry nameRASK_MOUSE
AccessionPrimary (citable) accession number: P32883
Secondary accession number(s): P04200, P08643
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot