P32881 (IFNA8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon alpha-8
Short name=IFN-alpha-8

Alternative name(s):
Interferon alpha-B
Short name=LeIF B
Interferon alpha-B2

Gene names

Name:

IFNA8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subcellular location	Secreted.
Sequence similarities	Belongs to the alpha/beta interferon family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW regulation of type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cytokine receptor binding Traceable author statement Ref.4. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.8
	Chain	24 – 189	166	Interferon alpha-8		PRO_0000016365
Amino acid modifications
	Disulfide bond	24 ↔ 122		By similarity
	Disulfide bond	52 ↔ 162		By similarity
Natural variations
	Natural variant	137	1	E → K. Corresponds to variant rs3739630 [ dbSNP | Ensembl ].		VAR_021975
Experimental info
	Sequence conflict	8	1	L → M in CAA23806. Ref.3
	Sequence conflict	8	1	L → M in CAA23811. Ref.4
	Sequence conflict	121 – 124	4	SCVM → VLCD in CAA23806. Ref.3
	Sequence conflict	121 – 124	4	SCVM → VLCD in CAA23811. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P32881 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 83128DA2B6DBB2C1	FASTA	189	21,989
	10 20 30 40 50 60 MALTFYLLVA LVVLSYKSFS SLGCDLPQTH SLGNRRALIL LAQMRRISPF SCLKDRHDFE 70 80 90 100 110 120 FPQEEFDDKQ FQKAQAISVL HEMIQQTFNL FSTKDSSAAL DETLLDEFYI ELDQQLNDLE 130 140 150 160 170 180 SCVMQEVGVI ESPLMYEDSI LAVRKYFQRI TLYLTEKKYS SCAWEVVRAE IMRSFSLSIN LQKRLKSKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural relationship of human interferon alpha genes and pseudogenes." Henco K., Brosius J., Fujisawa A., Fujisawa J., Haynes J.R., Hochstadt J., Kovacic T., Pasek M., Schamboeck A., Schmid J., Todokoro K., Waelchli M., Nagata S., Weissmann C. J. Mol. Biol. 185:227-260(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of eukaryotic genes in single-strand phage vectors: the human interferon genes." Bowden D.W., Mao J., Gill T., Hsiao K., Lillquist J.S., Testa D., Vovis G.F. Gene 27:87-99(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Bacterial synthesis of a novel human leukocyte interferon." Yelverton E., Leung D., Weck P., Gray P.W., Goeddel D.V. Nucleic Acids Res. 9:731-741(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The structure of eight distinct cloned human leukocyte interferon cDNAs." Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W. Nature 290:20-26(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes." Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N. Biochem. J. 329:295-302(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-53.
[9]	"Interferon-alpha 8b is the only variant of interferon-alpha 8 identified in a large human population." Hussain M., Gill D.S., Liao M.-J., Testa D. J. Interferon Cytokine Res. 16:523-529(1996) [PubMed] [Europe PMC] [Abstract] Cited for: ABSENCE OF POLYMORPHISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03125 Genomic DNA. Translation: CAA26903.1. K01900 mRNA. Translation: AAA52716.1. V00545 mRNA. Translation: CAA23806.1. V00550 mRNA. Translation: CAA23811.1. AL353732 Genomic DNA. Translation: CAH72909.1. CH471071 Genomic DNA. Translation: EAW58610.1. BC104828 mRNA. Translation: AAI04829.1. BC104830 mRNA. Translation: AAI04831.1.
IPI	IPI00031075.
PIR	IVHUA4. A93747. B61413. IVHUI8. D23753.
RefSeq	NP_002161.2. NM_002170.3.
UniGene	Hs.73890.
3D structure databases
ProteinModelPortal	P32881.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6017N.
STRING	9606.ENSP00000369553.
Polymorphism databases
DMDM	417188.
Proteomic databases
PaxDb	P32881.
PRIDE	P32881.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380205; ENSP00000369553; ENSG00000120242.
GeneID	3445.
KEGG	hsa:3445.
UCSC	uc003zpc.1. human.
Organism-specific databases
CTD	3445.
GeneCards	GC09P021409.
HGNC	HGNC:5429. IFNA8.
HPA	HPA047557.
MIM	147568. gene.
neXtProt	NX_P32881.
PharmGKB	PA29668. PA88.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG87565.
HOGENOM	HOG000230500.
HOVERGEN	HBG052086.
InParanoid	P32881.
KO	K05414.
OMA	VLSYKSF.
OrthoDB	EOG4JDH7V.
PhylomeDB	P32881.
Enzyme and pathway databases
Pathway_Interaction_DB	cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
Reactome	REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
CleanEx	HS_IFNA8.
Genevestigator	P32881.
GermOnline	ENSG00000120242. Homo sapiens.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR015589. Interferon_alpha. IPR000471. Interferon_alpha/beta/delta. [Graphical view]
PANTHER	PTHR11691. PTHR11691. 1 hit. PTHR11691:SF9. PTHR11691:SF9. 1 hit.
Pfam	PF00143. Interferon. 1 hit. [Graphical view]
PRINTS	PR00266. INTERFERONAB.
SMART	SM00076. IFabd. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00252. INTERFERON_A_B_D. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3445.
NextBio	13576.
SOURCE	Search...

Entry information

Entry name

IFNA8_HUMAN

Accession

Primary (citable) accession number: P32881
Secondary accession number(s): P01565 , P09236, Q5VWV7, Q5VYQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.