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P32875

- LIPA_YEAST

UniProt

P32875 - LIPA_YEAST

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Protein

Lipoyl synthase, mitochondrial

Gene
LIP5, YOR196C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives Inferred.2 Publications

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi150 – 1501Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi155 – 1551Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi161 – 1611Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi181 – 1811Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi185 – 1851Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi188 – 1881Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:YOR196C-MONOMER.
YEAST:YOR196C-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP5
Ordered Locus Names:YOR196C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR196c.
SGDiS000005722. LIP5.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818Mitochondrion Reviewed predictionAdd
BLAST
Chaini19 – 414396Lipoyl synthase, mitochondrialUniRule annotationPRO_0000017726Add
BLAST

Proteomic databases

MaxQBiP32875.
PaxDbiP32875.
PeptideAtlasiP32875.
PRIDEiP32875.

Expressioni

Gene expression databases

GenevestigatoriP32875.

Interactioni

Protein-protein interaction databases

BioGridi34594. 141 interactions.
DIPiDIP-3931N.
IntActiP32875. 1 interaction.
MINTiMINT-522178.
STRINGi4932.YOR196C.

Structurei

3D structure databases

ProteinModelPortaliP32875.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
GeneTreeiENSGT00390000006234.
KOiK03644.
OMAiEWLRRPI.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32875-1 [UniParc]FASTAAdd to Basket

« Hide

MYRRSVGVLF VGRNTRWISS TIRCGTSATR PIRSNALNTD SDNASVRVPV    50
GNSTEVENAT SQLTGTSGKR RKGNRKRITE FKDALNLGPS FADFVSGKAS 100
KMILDPLEKA RQNTEEAKKL PRWLKVPIPK GTNYHKLKGD VKELGLSTVC 150
EEARCPNIGE CWGGKDKSKA TATIMLLGDT CTRGCRFCSV KTNRTPSKPD 200
PMEPENTAEA IKRWGLGYVV LTTVDRDDLV DGGANHLAET VRKIKQKAPN 250
TLVETLSGDF RGDLKMVDIM AQCGLDVYAH NLETVESLTP HVRDRRATYR 300
QSLSVLERAK ATVPSLITKT SIMLGLGETD EQITQTLKDL RNIQCDVVTF 350
GQYMRPTKRH MKVVEYVKPE KFDYWKERAL EMGFLYCASG PLVRSSYKAG 400
EAFIENVLKK RNMK 414
Length:414
Mass (Da):46,247
Last modified:October 1, 1993 - v1
Checksum:i962BC3DF9857C3BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11999 Genomic DNA. Translation: AAA34745.1.
Z75104 Genomic DNA. Translation: CAA99409.1.
AY558046 Genomic DNA. Translation: AAS56372.1.
BK006948 Genomic DNA. Translation: DAA10971.1.
PIRiS42159.
RefSeqiNP_014839.1. NM_001183615.1.

Genome annotation databases

EnsemblFungiiYOR196C; YOR196C; YOR196C.
GeneIDi854371.
KEGGisce:YOR196C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11999 Genomic DNA. Translation: AAA34745.1 .
Z75104 Genomic DNA. Translation: CAA99409.1 .
AY558046 Genomic DNA. Translation: AAS56372.1 .
BK006948 Genomic DNA. Translation: DAA10971.1 .
PIRi S42159.
RefSeqi NP_014839.1. NM_001183615.1.

3D structure databases

ProteinModelPortali P32875.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34594. 141 interactions.
DIPi DIP-3931N.
IntActi P32875. 1 interaction.
MINTi MINT-522178.
STRINGi 4932.YOR196C.

Proteomic databases

MaxQBi P32875.
PaxDbi P32875.
PeptideAtlasi P32875.
PRIDEi P32875.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR196C ; YOR196C ; YOR196C .
GeneIDi 854371.
KEGGi sce:YOR196C.

Organism-specific databases

CYGDi YOR196c.
SGDi S000005722. LIP5.

Phylogenomic databases

eggNOGi COG0320.
GeneTreei ENSGT00390000006234.
KOi K03644.
OMAi EWLRRPI.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MetaCyc:YOR196C-MONOMER.
YEAST:YOR196C-MONOMER.

Miscellaneous databases

NextBioi 976499.
PROi P32875.

Gene expression databases

Genevestigatori P32875.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of LIP5. A lipoate biosynthetic locus of Saccharomyces cerevisiae."
    Sulo P., Martin N.C.
    J. Biol. Chem. 268:17634-17639(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: DBY745.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION.

Entry informationi

Entry nameiLIPA_YEAST
AccessioniPrimary (citable) accession number: P32875
Secondary accession number(s): D6W2Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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