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P32874

- HFA1_YEAST

UniProt

P32874 - HFA1_YEAST

Protein

Acetyl-CoA carboxylase, mitochondrial

Gene

HFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting reaction in the mitochondrial fatty acid synthesis (FAS) type II pathway. Responsible for the production of the mitochondrial malonyl-CoA, used for the biosynthesis of the cofactor lipoic acid. This protein carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase.1 Publication

    Catalytic activityi

    ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.
    ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].

    Cofactori

    Biotin.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei459 – 4591By similarity
    Binding sitei1776 – 17761Coenzyme ABy similarity
    Binding sitei2080 – 20801Coenzyme ABy similarity
    Binding sitei2082 – 20821Coenzyme ABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi332 – 3376ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. acetyl-CoA carboxylase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. biotin carboxylase activity Source: UniProtKB-EC
    4. metal ion binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: SGD
    2. malonyl-CoA biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Biotin, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR207C-MONOMER.
    YEAST:YMR207C-MONOMER.
    ReactomeiREACT_188404. Defective HLCS causes multiple carboxylase deficiency.
    REACT_188774. Biotin transport and metabolism.
    UniPathwayiUPA00655; UER00711.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-CoA carboxylase, mitochondrial (EC:6.4.1.2)
    Short name:
    ACC
    Including the following 1 domains:
    Biotin carboxylase (EC:6.3.4.14)
    Gene namesi
    Name:HFA1
    Ordered Locus Names:YMR207C
    ORF Names:YM8261.01C, YM8325.08C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004820. HFA1.

    Subcellular locationi

    Mitochondrion 3 Publications

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 104104MitochondrionSequence AnalysisAdd
    BLAST
    Chaini105 – 22732169Acetyl-CoA carboxylase, mitochondrialPRO_0000146771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei804 – 8041N6-biotinyllysineBy similarity

    Proteomic databases

    MaxQBiP32874.
    PaxDbiP32874.

    Expressioni

    Gene expression databases

    GenevestigatoriP32874.

    Interactioni

    Protein-protein interaction databases

    BioGridi35385. 82 interactions.
    DIPiDIP-2568N.
    IntActiP32874. 2 interactions.
    MINTiMINT-423824.

    Structurei

    3D structure databases

    ProteinModelPortaliP32874.
    SMRiP32874. Positions 90-634, 1538-2236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 635502Biotin carboxylationAdd
    BLAST
    Domaini292 – 484193ATP-graspPROSITE-ProRule annotationAdd
    BLAST
    Domaini770 – 83667Biotinyl-bindingAdd
    BLAST
    Domaini1648 – 2147500CarboxyltransferaseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
    Contains 1 biotin carboxylation domain.Curated
    Contains 1 biotinyl-binding domain.Curated
    Contains 1 carboxyltransferase domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0511.
    HOGENOMiHOG000214115.
    OrthoDBiEOG74J9H5.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.226.10. 3 hits.
    InterProiIPR013537. AcCoA_COase_cen.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR000022. Carboxyl_trans.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR029045. ClpP/crotonase-like_dom.
    IPR011763. COA_CT_C.
    IPR011762. COA_CT_N.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PfamiPF08326. ACC_central. 1 hit.
    PF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF01039. Carboxyl_trans. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view]
    SMARTiSM00878. Biotin_carb_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52096. SSF52096. 2 hits.
    SSF52440. SSF52440. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS50989. COA_CT_CTER. 1 hit.
    PS50980. COA_CT_NTER. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32874-1 [UniParc]FASTAAdd to Basket

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    KGKTITHGQS WGARRIHSHF YITIFTITCI RIGQYKLALY LDPYRFYNIT     50
    GSQIVRLKGQ RPEYRKRIFA HSYRHSSRIG LNFPSRRRYS NYVDRGNIHK 100
    HTRLPPQFIG LNTVESAQPS ILRDFVDLRG GHTVISKILI ANNGIAAVKE 150
    MRSIRKWAYE TFNDEKIIQF VVMATPDDLH ANSEYIRMAD QYVQVPGGTN 200
    NNNYANIDLI LDVAEQTDVD AVWAGWGHAS ENPCLPELLA SSQRKILFIG 250
    PPGRAMRSLG DKISSTIVAQ SAKIPCIPWS GSHIDTIHID NKTNFVSVPD 300
    DVYVRGCCSS PEDALEKAKL IGFPVMIKAS EGGGGKGIRR VDNEDDFIAL 350
    YRQAVNETPG SPMFVMKVVT DARHLEVQLL ADQYGTNITL FGRDCSIQRR 400
    HQKIIEEAPV TITKPETFQR MERAAIRLGE LVGYVSAGTV EYLYSPKDDK 450
    FYFLELNPRL QVEHPTTEMI SGVNLPATQL QIAMGIPMHM ISDIRKLYGL 500
    DPTGTSYIDF KNLKRPSPKG HCISCRITSE DPNEGFKPST GKIHELNFRS 550
    SSNVWGYFSV GNNGAIHSFS DSQFGHIFAV GNDRQDAKQN MVLALKDFSI 600
    RGEFKTPIEY LIELLETRDF ESNNISTGWL DDLILKNLSS DSKLDPTLAI 650
    ICGAAMKAYV FTEKVRNKYL ELLRRGQVPP KDFLKTKFPV DFIFDNNRYL 700
    FNVAQSSEEQ FILSINKSQC EVNVQKLSSD CLLISVDGKC HTVYWKDDIR 750
    GTRLSIDSNT IFLEAELNPT QVISPTPGKL VKYLVRSGDH VFAGQQYAEI 800
    EIMKMQMPLV AKSDGVIELL RQPGSIIEAG DVIAKLTLDS PSKANESSLY 850
    RGELPVLGPP LIEGSRPNHK LRVLINRLEN ILNGYHENSG IETTLKELIK 900
    ILRDGRLPYS EWDSQISTVR NRLPRQLNEG LGNLVKKSVS FPAKELHKLM 950
    KRYLEENTND HVVYVALQPL LKISERYSEG LANHECEIFL KLIKKYYAVE 1000
    KIFENHDIHE ERNLLNLRRK DLTNLKKILC ISLSHANVVA KNKLVTAILH 1050
    EYEPLCQDSS KMSLKFRAVI HDLASLESKW AKEVAVKARS VLLRGIFPPI 1100
    KKRKEHIKTL LQLHIKDTGA ENIHSRNIYS CMRDFGNLIH SNLIQLQDLF 1150
    FFFGHQDTAL SSIASEIYAR YAYGNYQLKS IKIHKGAPDL LMSWQFSSLR 1200
    NYLVNSDGES DEFTKLSKPP STSGKSSANS FGLLVNMRAL ESLEKTLDEV 1250
    YEQIHIPEER LSSGENSLIV NILSPIRYRS ENDLIKTLKI KLHENERGLS 1300
    KLKVNRITFA FIAANAPAVK FYSFDGTTYD EISQIRNMDP SYEAPLELGK 1350
    MSNYKIRSLP TYDSSIRIFE GISKFTPLDK RFFVRKIINS FMYNDQKTTE 1400
    ENLKAEINAQ VVYMLEHLGA VDISNSDLNH IFLSFNTVLN IPVHRLEEIV 1450
    STILKTHETR LFQERITDVE ICISVECLET KKPAPLRLLI SNKSGYVVKI 1500
    ETYYEKIGKN GNLILEPCSE QSHYSQKSLS LPYSVKDWLQ PKRYKAQFMG 1550
    TTYVYDFPGL FHQAAIQQWK RYFPKHKLND SFFSWVELIE QNGNLIKVNR 1600
    EPGLNNIGMV AFEIMVQTPE YPEGRNMIVI SNDITYNIGS FGPREDLFFD 1650
    RVTNYARERG IPRIYLAANS GAKLGIAEEL IPLFRVAWND PSDPTKGFQY 1700
    LYLAPKDMQL LKDSGKGNSV VVEHKMVYGE ERYIIKAIVG FEEGLGVECL 1750
    QGSGLIAGAT SKAYRDIFTI TAVTCRSVGI GSYLVRLGQR TIQVEDKPII 1800
    LTGASAINKV LGTDIYTSNL QIGGTQIMYK NGIAHLTASN DMKAIEKIMT 1850
    WLSYVPAKRD MSPPLLETMD RWDRDVDFKP AKQVPYEARW LIEGKWDSNN 1900
    NFQSGLFDKD SFFETLSGWA KGVIVGRARL GGIPVGVIAV ETKTIEEIIP 1950
    ADPANLDSSE FSVKEAGQVW YPNSAFKTAQ TINDFNYGEQ LPLIILANWR 2000
    GFSGGQRDMY NEVLKYGSFI VDALVDYKQP ILIYIPPFGE LRGGSWVVID 2050
    PTINPEQMEM YADVESRGGV LEPDGVVSIK YRKEKMIETM IRLDSTYGHL 2100
    RRTLTEKKLS LEKQNDLTKR LKIRERQLIP IYNQISIQFA DLHDRSTRML 2150
    VKGVIRNELE WKKSRRFLYW RLRRRLNEGQ VIKRLQKKTC DNKTKMKYDD 2200
    LLKIVQSWYN DLDVNDDRAV VEFIERNSKK IDKNIEEFEI SLLIDELKKK 2250
    FEDRRGNIVL EELTRLVDSK RKR 2273
    Length:2,273
    Mass (Da):259,163
    Last modified:October 1, 1996 - v2
    Checksum:i08727A301549DA92
    GO

    Sequence cautioni

    The sequence DAA10106.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti661 – 6611F → L in CAA80280. (PubMed:7906156)Curated
    Sequence conflicti1027 – 10271K → E in BAA24410. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78165 Genomic DNA. Translation: BAA24410.1.
    Z49809 Genomic DNA. Translation: CAA89922.1.
    Z48755 Genomic DNA. Translation: CAA88647.1.
    Z22558 Genomic DNA. Translation: CAA80280.1.
    BK006946 Genomic DNA. Translation: DAA10106.1. Different initiation.
    PIRiS55089.
    RefSeqiNP_013934.1. NM_001182714.1.

    Genome annotation databases

    GeneIDi855247.
    KEGGisce:YMR207C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78165 Genomic DNA. Translation: BAA24410.1 .
    Z49809 Genomic DNA. Translation: CAA89922.1 .
    Z48755 Genomic DNA. Translation: CAA88647.1 .
    Z22558 Genomic DNA. Translation: CAA80280.1 .
    BK006946 Genomic DNA. Translation: DAA10106.1 . Different initiation.
    PIRi S55089.
    RefSeqi NP_013934.1. NM_001182714.1.

    3D structure databases

    ProteinModelPortali P32874.
    SMRi P32874. Positions 90-634, 1538-2236.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35385. 82 interactions.
    DIPi DIP-2568N.
    IntActi P32874. 2 interactions.
    MINTi MINT-423824.

    Proteomic databases

    MaxQBi P32874.
    PaxDbi P32874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 855247.
    KEGGi sce:YMR207C.

    Organism-specific databases

    SGDi S000004820. HFA1.

    Phylogenomic databases

    eggNOGi COG0511.
    HOGENOMi HOG000214115.
    OrthoDBi EOG74J9H5.

    Enzyme and pathway databases

    UniPathwayi UPA00655 ; UER00711 .
    BioCyci MetaCyc:YMR207C-MONOMER.
    YEAST:YMR207C-MONOMER.
    Reactomei REACT_188404. Defective HLCS causes multiple carboxylase deficiency.
    REACT_188774. Biotin transport and metabolism.

    Miscellaneous databases

    NextBioi 978813.

    Gene expression databases

    Genevestigatori P32874.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.226.10. 3 hits.
    InterProi IPR013537. AcCoA_COase_cen.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR000022. Carboxyl_trans.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR029045. ClpP/crotonase-like_dom.
    IPR011763. COA_CT_C.
    IPR011762. COA_CT_N.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    Pfami PF08326. ACC_central. 1 hit.
    PF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF01039. Carboxyl_trans. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view ]
    SMARTi SM00878. Biotin_carb_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52096. SSF52096. 2 hits.
    SSF52440. SSF52440. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS50989. COA_CT_CTER. 1 hit.
    PS50980. COA_CT_NTER. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Occurrence of an acetyl-CoA carboxylase-like gene in Saccharomyces serevisiae."
      Saito A., Kazuta Y., Kondo H., Tanabe T.
      Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SP1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Identification of a Saccharomyces cerevisiae gene closely related to FAS3 (acetyl-CoA carboxylase)."
      Kearsey S.E.
      DNA Seq. 4:69-70(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-949.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae."
      Hoja U., Marthol S., Hofmann J., Stegner S., Schulz R., Meier S., Greiner E., Schweizer E.
      J. Biol. Chem. 279:21779-21786(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiHFA1_YEAST
    AccessioniPrimary (citable) accession number: P32874
    Secondary accession number(s): D6W032, O42823
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 396 molecules/cell in log phase SD medium.1 Publication

    Caution

    The reading frame from which this protein is translated has no Met initiation codon near to the 5'-end. However, it is not a pseudogene. It has been shown (PubMed:14761959) that at least 72 residues upstream of the first in-frame start codon (Met-151) are required for function and proper subcellular location. May be translated by means of alternative initiation codon usage, programmed translational frame shifting, or mRNA editing.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3