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P32873

- BEM3_YEAST

UniProt

P32873 - BEM3_YEAST

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Protein

GTPase-activating protein BEM3

Gene

BEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase.2 Publications

GO - Molecular functioni

  1. phosphatidylinositol-3-phosphate binding Source: SGD
  2. Rho GTPase activator activity Source: SGD

GO - Biological processi

  1. establishment of cell polarity Source: SGD
  2. negative regulation of Rho protein signal transduction Source: SGD
  3. positive regulation of Rho GTPase activity Source: GOC
  4. septin ring organization Source: SGD
  5. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-34015-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein BEM3
Alternative name(s):
Bud emergence protein 3
Gene namesi
Name:BEM3
Ordered Locus Names:YPL115C
ORF Names:LPH12C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL115c.
SGDiS000006036. BEM3.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: SGD
  4. incipient cellular bud site Source: SGD
  5. mating projection tip Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11281128GTPase-activating protein BEM3PRO_0000056728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei254 – 2541Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32873.
PaxDbiP32873.
PeptideAtlasiP32873.

Expressioni

Gene expression databases

GenevestigatoriP32873.

Interactioni

Protein-protein interaction databases

BioGridi36066. 80 interactions.
DIPiDIP-2575N.
IntActiP32873. 7 interactions.
MINTiMINT-423650.
STRINGi4932.YPL115C.

Structurei

3D structure databases

ProteinModelPortaliP32873.
SMRiP32873. Positions 900-1089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini634 – 741108PHPROSITE-ProRule annotationAdd
BLAST
Domaini913 – 1128216Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG295787.
HOGENOMiHOG000095196.
InParanoidiP32873.
OMAiPLFVQPE.
OrthoDBiEOG7DC2CS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00787. PX. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00312. PX. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32873 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD
60 70 80 90 100
EIFTENIKLK LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR
110 120 130 140 150
DSYVNDQSFV LPPRSAERKA HIKSLNLPIP TLSPPLQQGS DVALETSVTP
160 170 180 190 200
TVPQIGVTSN TSISRKHLQN MILNDEIEAN SSFSSPKIIN RSVSSPTKIH
210 220 230 240 250
SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN PNRMTAVINN
260 270 280 290 300
HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP
310 320 330 340 350
TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT
360 370 380 390 400
DSKDPLSIKS TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL
410 420 430 440 450
NKKDDNFGAQ SAKNLKKPVL TSSLPNLSTK LSTTSQNASL PPNPPVESSS
460 470 480 490 500
KQKQLGETAS IHSTNTLNTF SSTPQGSLKT LRRPHASSVS TVKSVAQSLK
510 520 530 540 550
SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR KSGKEMFKFS
560 570 580 590 600
KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY
610 620 630 640 650
TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT
660 670 680 690 700
GNSTWRVRYG ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR
710 720 730 740 750
NGFLITEHKK SGLSTSTKYY ICTETSKERE LWLSAFSDYI DPSQSLSLSS
760 770 780 790 800
SRNANDTDSA SHLSAGTHHS KFGNATISAT DTPSYVTDLT QEYNNNNNIS
810 820 830 840 850
NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK MRSLFPFKKL
860 870 880 890 900
TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV
910 920 930 940 950
STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR
960 970 980 990 1000
LSGSSTVIKT LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG
1010 1020 1030 1040 1050
LLKLYLRKLP HLLFGDEQFL SFKRVVDENH NNPVQISLGF KELIESGLVP
1060 1070 1080 1090 1100
HANLSLMYAL FELLVRINEN SKFNKMNLRN LCIVFSPTLN IPISMLQPFI
1110 1120
TDFACIFQGG EPVKEEEREK VDIHIPQV
Length:1,128
Mass (Da):124,913
Last modified:November 1, 1997 - v3
Checksum:i4BF03EAD6EF10283
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14558 Genomic DNA. Translation: AAA34453.1.
U43503 Genomic DNA. Translation: AAB68247.1.
BK006949 Genomic DNA. Translation: DAA11319.1.
PIRiA49960.
RefSeqiNP_015210.1. NM_001183929.1.

Genome annotation databases

EnsemblFungiiYPL115C; YPL115C; YPL115C.
GeneIDi855988.
KEGGisce:YPL115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14558 Genomic DNA. Translation: AAA34453.1 .
U43503 Genomic DNA. Translation: AAB68247.1 .
BK006949 Genomic DNA. Translation: DAA11319.1 .
PIRi A49960.
RefSeqi NP_015210.1. NM_001183929.1.

3D structure databases

ProteinModelPortali P32873.
SMRi P32873. Positions 900-1089.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36066. 80 interactions.
DIPi DIP-2575N.
IntActi P32873. 7 interactions.
MINTi MINT-423650.
STRINGi 4932.YPL115C.

Proteomic databases

MaxQBi P32873.
PaxDbi P32873.
PeptideAtlasi P32873.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL115C ; YPL115C ; YPL115C .
GeneIDi 855988.
KEGGi sce:YPL115C.

Organism-specific databases

CYGDi YPL115c.
SGDi S000006036. BEM3.

Phylogenomic databases

eggNOGi NOG295787.
HOGENOMi HOG000095196.
InParanoidi P32873.
OMAi PLFVQPE.
OrthoDBi EOG7DC2CS.

Enzyme and pathway databases

BioCyci YEAST:G3O-34015-MONOMER.

Miscellaneous databases

NextBioi 980837.

Gene expression databases

Genevestigatori P32873.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
PF00787. PX. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00312. PX. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3."
    Zheng Y., Cerione R., Bender A.
    J. Biol. Chem. 269:2369-2372(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain."
    Zheng Y., Hart M.J., Shinjo K., Evans T., Bender A., Cerione R.A.
    J. Biol. Chem. 268:24629-24634(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBEM3_YEAST
AccessioniPrimary (citable) accession number: P32873
Secondary accession number(s): D6W3Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3