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Protein

GTPase-activating protein BEM3

Gene

BEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase.2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • establishment of cell polarity Source: SGD
  • negative regulation of Rho protein signal transduction Source: SGD
  • septin ring organization Source: SGD
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-34015-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein BEM3
Alternative name(s):
Bud emergence protein 3
Gene namesi
Name:BEM3
Ordered Locus Names:YPL115C
ORF Names:LPH12C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL115C.
SGDiS000006036. BEM3.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11281128GTPase-activating protein BEM3PRO_0000056728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei254 – 2541PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32873.

PTM databases

iPTMnetiP32873.

Interactioni

Protein-protein interaction databases

BioGridi36066. 83 interactions.
DIPiDIP-2575N.
IntActiP32873. 7 interactions.
MINTiMINT-423650.

Structurei

3D structure databases

ProteinModelPortaliP32873.
SMRiP32873. Positions 900-1089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini634 – 741108PHPROSITE-ProRule annotationAdd
BLAST
Domaini913 – 1128216Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000095196.
InParanoidiP32873.
KOiK19840.
OMAiRCLEYLY.
OrthoDBiEOG092C047S.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00787. PX. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00312. PX. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD
60 70 80 90 100
EIFTENIKLK LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR
110 120 130 140 150
DSYVNDQSFV LPPRSAERKA HIKSLNLPIP TLSPPLQQGS DVALETSVTP
160 170 180 190 200
TVPQIGVTSN TSISRKHLQN MILNDEIEAN SSFSSPKIIN RSVSSPTKIH
210 220 230 240 250
SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN PNRMTAVINN
260 270 280 290 300
HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP
310 320 330 340 350
TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT
360 370 380 390 400
DSKDPLSIKS TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL
410 420 430 440 450
NKKDDNFGAQ SAKNLKKPVL TSSLPNLSTK LSTTSQNASL PPNPPVESSS
460 470 480 490 500
KQKQLGETAS IHSTNTLNTF SSTPQGSLKT LRRPHASSVS TVKSVAQSLK
510 520 530 540 550
SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR KSGKEMFKFS
560 570 580 590 600
KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY
610 620 630 640 650
TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT
660 670 680 690 700
GNSTWRVRYG ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR
710 720 730 740 750
NGFLITEHKK SGLSTSTKYY ICTETSKERE LWLSAFSDYI DPSQSLSLSS
760 770 780 790 800
SRNANDTDSA SHLSAGTHHS KFGNATISAT DTPSYVTDLT QEYNNNNNIS
810 820 830 840 850
NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK MRSLFPFKKL
860 870 880 890 900
TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV
910 920 930 940 950
STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR
960 970 980 990 1000
LSGSSTVIKT LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG
1010 1020 1030 1040 1050
LLKLYLRKLP HLLFGDEQFL SFKRVVDENH NNPVQISLGF KELIESGLVP
1060 1070 1080 1090 1100
HANLSLMYAL FELLVRINEN SKFNKMNLRN LCIVFSPTLN IPISMLQPFI
1110 1120
TDFACIFQGG EPVKEEEREK VDIHIPQV
Length:1,128
Mass (Da):124,913
Last modified:November 1, 1997 - v3
Checksum:i4BF03EAD6EF10283
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14558 Genomic DNA. Translation: AAA34453.1.
U43503 Genomic DNA. Translation: AAB68247.1.
BK006949 Genomic DNA. Translation: DAA11319.1.
PIRiA49960.
RefSeqiNP_015210.1. NM_001183929.1.

Genome annotation databases

EnsemblFungiiYPL115C; YPL115C; YPL115C.
GeneIDi855988.
KEGGisce:YPL115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14558 Genomic DNA. Translation: AAA34453.1.
U43503 Genomic DNA. Translation: AAB68247.1.
BK006949 Genomic DNA. Translation: DAA11319.1.
PIRiA49960.
RefSeqiNP_015210.1. NM_001183929.1.

3D structure databases

ProteinModelPortaliP32873.
SMRiP32873. Positions 900-1089.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36066. 83 interactions.
DIPiDIP-2575N.
IntActiP32873. 7 interactions.
MINTiMINT-423650.

PTM databases

iPTMnetiP32873.

Proteomic databases

MaxQBiP32873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL115C; YPL115C; YPL115C.
GeneIDi855988.
KEGGisce:YPL115C.

Organism-specific databases

EuPathDBiFungiDB:YPL115C.
SGDiS000006036. BEM3.

Phylogenomic databases

HOGENOMiHOG000095196.
InParanoidiP32873.
KOiK19840.
OMAiRCLEYLY.
OrthoDBiEOG092C047S.

Enzyme and pathway databases

BioCyciYEAST:G3O-34015-MONOMER.

Miscellaneous databases

PROiP32873.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00787. PX. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00312. PX. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBEM3_YEAST
AccessioniPrimary (citable) accession number: P32873
Secondary accession number(s): D6W3Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.