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P32873

- BEM3_YEAST

UniProt

P32873 - BEM3_YEAST

Protein

GTPase-activating protein BEM3

Gene

BEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase.2 Publications

    GO - Molecular functioni

    1. phosphatidylinositol-3-phosphate binding Source: SGD
    2. Rho GTPase activator activity Source: SGD

    GO - Biological processi

    1. establishment of cell polarity Source: SGD
    2. negative regulation of Rho protein signal transduction Source: SGD
    3. positive regulation of Rho GTPase activity Source: GOC
    4. septin ring organization Source: SGD
    5. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34015-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase-activating protein BEM3
    Alternative name(s):
    Bud emergence protein 3
    Gene namesi
    Name:BEM3
    Ordered Locus Names:YPL115C
    ORF Names:LPH12C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL115c.
    SGDiS000006036. BEM3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: SGD
    4. incipient cellular bud site Source: SGD
    5. mating projection tip Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11281128GTPase-activating protein BEM3PRO_0000056728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei254 – 2541Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32873.
    PaxDbiP32873.
    PeptideAtlasiP32873.

    Expressioni

    Gene expression databases

    GenevestigatoriP32873.

    Interactioni

    Protein-protein interaction databases

    BioGridi36066. 79 interactions.
    DIPiDIP-2575N.
    IntActiP32873. 7 interactions.
    MINTiMINT-423650.
    STRINGi4932.YPL115C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32873.
    SMRiP32873. Positions 900-1089.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini634 – 741108PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini913 – 1128216Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG295787.
    HOGENOMiHOG000095196.
    OMAiPLFVQPE.
    OrthoDBiEOG7DC2CS.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001683. Phox.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF00787. PX. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00312. PX. 1 hit.
    SM00324. RhoGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32873-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD     50
    EIFTENIKLK LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR 100
    DSYVNDQSFV LPPRSAERKA HIKSLNLPIP TLSPPLQQGS DVALETSVTP 150
    TVPQIGVTSN TSISRKHLQN MILNDEIEAN SSFSSPKIIN RSVSSPTKIH 200
    SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN PNRMTAVINN 250
    HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP 300
    TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT 350
    DSKDPLSIKS TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL 400
    NKKDDNFGAQ SAKNLKKPVL TSSLPNLSTK LSTTSQNASL PPNPPVESSS 450
    KQKQLGETAS IHSTNTLNTF SSTPQGSLKT LRRPHASSVS TVKSVAQSLK 500
    SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR KSGKEMFKFS 550
    KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY 600
    TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT 650
    GNSTWRVRYG ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR 700
    NGFLITEHKK SGLSTSTKYY ICTETSKERE LWLSAFSDYI DPSQSLSLSS 750
    SRNANDTDSA SHLSAGTHHS KFGNATISAT DTPSYVTDLT QEYNNNNNIS 800
    NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK MRSLFPFKKL 850
    TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV 900
    STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR 950
    LSGSSTVIKT LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG 1000
    LLKLYLRKLP HLLFGDEQFL SFKRVVDENH NNPVQISLGF KELIESGLVP 1050
    HANLSLMYAL FELLVRINEN SKFNKMNLRN LCIVFSPTLN IPISMLQPFI 1100
    TDFACIFQGG EPVKEEEREK VDIHIPQV 1128
    Length:1,128
    Mass (Da):124,913
    Last modified:November 1, 1997 - v3
    Checksum:i4BF03EAD6EF10283
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14558 Genomic DNA. Translation: AAA34453.1.
    U43503 Genomic DNA. Translation: AAB68247.1.
    BK006949 Genomic DNA. Translation: DAA11319.1.
    PIRiA49960.
    RefSeqiNP_015210.1. NM_001183929.1.

    Genome annotation databases

    EnsemblFungiiYPL115C; YPL115C; YPL115C.
    GeneIDi855988.
    KEGGisce:YPL115C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14558 Genomic DNA. Translation: AAA34453.1 .
    U43503 Genomic DNA. Translation: AAB68247.1 .
    BK006949 Genomic DNA. Translation: DAA11319.1 .
    PIRi A49960.
    RefSeqi NP_015210.1. NM_001183929.1.

    3D structure databases

    ProteinModelPortali P32873.
    SMRi P32873. Positions 900-1089.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36066. 79 interactions.
    DIPi DIP-2575N.
    IntActi P32873. 7 interactions.
    MINTi MINT-423650.
    STRINGi 4932.YPL115C.

    Proteomic databases

    MaxQBi P32873.
    PaxDbi P32873.
    PeptideAtlasi P32873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL115C ; YPL115C ; YPL115C .
    GeneIDi 855988.
    KEGGi sce:YPL115C.

    Organism-specific databases

    CYGDi YPL115c.
    SGDi S000006036. BEM3.

    Phylogenomic databases

    eggNOGi NOG295787.
    HOGENOMi HOG000095196.
    OMAi PLFVQPE.
    OrthoDBi EOG7DC2CS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34015-MONOMER.

    Miscellaneous databases

    NextBioi 980837.

    Gene expression databases

    Genevestigatori P32873.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001683. Phox.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF00787. PX. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00312. PX. 1 hit.
    SM00324. RhoGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3."
      Zheng Y., Cerione R., Bender A.
      J. Biol. Chem. 269:2369-2372(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain."
      Zheng Y., Hart M.J., Shinjo K., Evans T., Bender A., Cerione R.A.
      J. Biol. Chem. 268:24629-24634(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBEM3_YEAST
    AccessioniPrimary (citable) accession number: P32873
    Secondary accession number(s): D6W3Q3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 752 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3