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P32873 (BEM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase-activating protein BEM3
Alternative name(s):
Bud emergence protein 3
Gene names
Name:BEM3
Ordered Locus Names:YPL115C
ORF Names:LPH12C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase. Ref.1 Ref.4

Subcellular location

Cytoplasm.

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11281128GTPase-activating protein BEM3
PRO_0000056728

Regions

Domain634 – 741108PH
Domain913 – 1128216Rho-GAP

Amino acid modifications

Modified residue2541Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P32873 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 4BF03EAD6EF10283

FASTA1,128124,913
        10         20         30         40         50         60 
MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD EIFTENIKLK 

        70         80         90        100        110        120 
LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR DSYVNDQSFV LPPRSAERKA 

       130        140        150        160        170        180 
HIKSLNLPIP TLSPPLQQGS DVALETSVTP TVPQIGVTSN TSISRKHLQN MILNDEIEAN 

       190        200        210        220        230        240 
SSFSSPKIIN RSVSSPTKIH SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN 

       250        260        270        280        290        300 
PNRMTAVINN HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP 

       310        320        330        340        350        360 
TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT DSKDPLSIKS 

       370        380        390        400        410        420 
TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL NKKDDNFGAQ SAKNLKKPVL 

       430        440        450        460        470        480 
TSSLPNLSTK LSTTSQNASL PPNPPVESSS KQKQLGETAS IHSTNTLNTF SSTPQGSLKT 

       490        500        510        520        530        540 
LRRPHASSVS TVKSVAQSLK SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR 

       550        560        570        580        590        600 
KSGKEMFKFS KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY 

       610        620        630        640        650        660 
TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT GNSTWRVRYG 

       670        680        690        700        710        720 
ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR NGFLITEHKK SGLSTSTKYY 

       730        740        750        760        770        780 
ICTETSKERE LWLSAFSDYI DPSQSLSLSS SRNANDTDSA SHLSAGTHHS KFGNATISAT 

       790        800        810        820        830        840 
DTPSYVTDLT QEYNNNNNIS NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK 

       850        860        870        880        890        900 
MRSLFPFKKL TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV 

       910        920        930        940        950        960 
STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR LSGSSTVIKT 

       970        980        990       1000       1010       1020 
LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG LLKLYLRKLP HLLFGDEQFL 

      1030       1040       1050       1060       1070       1080 
SFKRVVDENH NNPVQISLGF KELIESGLVP HANLSLMYAL FELLVRINEN SKFNKMNLRN 

      1090       1100       1110       1120 
LCIVFSPTLN IPISMLQPFI TDFACIFQGG EPVKEEEREK VDIHIPQV 

« Hide

References

« Hide 'large scale' references
[1]"Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3."
Zheng Y., Cerione R., Bender A.
J. Biol. Chem. 269:2369-2372(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain."
Zheng Y., Hart M.J., Shinjo K., Evans T., Bender A., Cerione R.A.
J. Biol. Chem. 268:24629-24634(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L14558 Genomic DNA. Translation: AAA34453.1.
U43503 Genomic DNA. Translation: AAB68247.1.
BK006949 Genomic DNA. Translation: DAA11319.1.
PIRA49960.
RefSeqNP_015210.1. NM_001183929.1.

3D structure databases

ProteinModelPortalP32873.
SMRP32873. Positions 636-738, 870-1107.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36066. 79 interactions.
DIPDIP-2575N.
IntActP32873. 7 interactions.
MINTMINT-423650.
STRING4932.YPL115C.

Proteomic databases

PaxDbP32873.
PeptideAtlasP32873.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL115C; YPL115C; YPL115C.
GeneID855988.
KEGGsce:YPL115C.

Organism-specific databases

CYGDYPL115c.
SGDS000006036. BEM3.

Phylogenomic databases

eggNOGNOG295787.
HOGENOMHOG000095196.
OMAPLFVQPE.
OrthoDBEOG7DC2CS.

Enzyme and pathway databases

BioCycYEAST:G3O-34015-MONOMER.

Gene expression databases

GenevestigatorP32873.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001683. Phox.
IPR001849. Pleckstrin_homology.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF00787. PX. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00312. PX. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980837.

Entry information

Entry nameBEM3_YEAST
AccessionPrimary (citable) accession number: P32873
Secondary accession number(s): D6W3Q3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families