P32873 (BEM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase-activating protein BEM3

Alternative name(s):
Bud emergence protein 3

Gene names

Name:	BEM3
Ordered Locus Names:	YPL115C
ORF Names:	LPH12C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1128 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase. Ref.1 Ref.4
Subcellular location	Cytoplasm.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 PH domain. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of cell polarity Traceable author statement PubMed 9891811. Source: SGD negative regulation of Rho protein signal transduction Inferred from mutant phenotype PubMed 17914457. Source: SGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cell cortex Inferred from direct assay PubMed 17914457. Source: SGD cellular bud tip Inferred from direct assay PubMed 17914457. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 17914457. Source: SGD mating projection tip Inferred from direct assay PubMed 17914457PubMed 19053807. Source: SGD
Molecular_function	Rho GTPase activator activity Inferred from direct assay PubMed 12455995Ref.4. Source: SGD phosphatidylinositol-3-phosphate binding Inferred from direct assay PubMed 11557775. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1128	1128	GTPase-activating protein BEM3		PRO_0000056728
Regions
	Domain	634 – 741	108	PH
	Domain	913 – 1128	216	Rho-GAP
Amino acid modifications
	Modified residue	194	1	Phosphoserine Ref.6
	Modified residue	324	1	Phosphoserine Ref.6 Ref.8
	Modified residue	423	1	Phosphoserine Ref.7 Ref.8
	Modified residue	488	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P32873 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 4BF03EAD6EF10283	FASTA	1,128	124,913
	10 20 30 40 50 60 MTDNLTTTHG GSTTLELLAQ YNDHRSKKDK SIEHIEKGTC SGKERNPSYD EIFTENIKLK 70 80 90 100 110 120 LQVQEYETEI ESLEKVIDML QKNREASLEV VLEQVQNDSR DSYVNDQSFV LPPRSAERKA 130 140 150 160 170 180 HIKSLNLPIP TLSPPLQQGS DVALETSVTP TVPQIGVTSN TSISRKHLQN MILNDEIEAN 190 200 210 220 230 240 SSFSSPKIIN RSVSSPTKIH SEQLASPAAS VTYTTSRITI KSPNKGSKSP LQERLRSPQN 250 260 270 280 290 300 PNRMTAVINN HLHSPLKAST SNNLDELTES KSQQLTNDAI QKNDRVYSSI TSSAYTTGTP 310 320 330 340 350 360 TSAAKSPSSL LEVKEGENKA LGFSPASKEK LDDFTQLLDS SFGEEDLVNT DSKDPLSIKS 370 380 390 400 410 420 TINESLPPPP APPTFFSPTS SGNIKNSTPL SSHLASPVIL NKKDDNFGAQ SAKNLKKPVL 430 440 450 460 470 480 TSSLPNLSTK LSTTSQNASL PPNPPVESSS KQKQLGETAS IHSTNTLNTF SSTPQGSLKT 490 500 510 520 530 540 LRRPHASSVS TVKSVAQSLK SDIPLFVQPE DFGTIQIEVL STLYRDNEDD LSILIAIIDR 550 560 570 580 590 600 KSGKEMFKFS KSIHKVRELD VYMKSHVPDL PLPTLPDRQL FQTLSPTKVD TRKNILNQYY 610 620 630 640 650 660 TSIFSVPEFP KNVGLKIAQF ISTDTVMTPP MMDDNVKDGS LLLRRPKTLT GNSTWRVRYG 670 680 690 700 710 720 ILRDDVLQLF DKNQLTETIK LRQSSIELIP NLPEDRFGTR NGFLITEHKK SGLSTSTKYY 730 740 750 760 770 780 ICTETSKERE LWLSAFSDYI DPSQSLSLSS SRNANDTDSA SHLSAGTHHS KFGNATISAT 790 800 810 820 830 840 DTPSYVTDLT QEYNNNNNIS NSSNNIANSD GIDSNPSSHS NFLASSSGNA EEEKDSRRAK 850 860 870 880 890 900 MRSLFPFKKL TGPASAMNHI GITISNDSDS PTSPDSIIKS PSKKLMEVSS SSNSSTGPHV 910 920 930 940 950 960 STAIFGSSLE TCLRLSSHKY QNVYDLPSVV YRCLEYLYKN RGIQEEGIFR LSGSSTVIKT 970 980 990 1000 1010 1020 LQERFDKEYD VDLCRYNESI EAKDDEASPS LYIGVNTVSG LLKLYLRKLP HLLFGDEQFL 1030 1040 1050 1060 1070 1080 SFKRVVDENH NNPVQISLGF KELIESGLVP HANLSLMYAL FELLVRINEN SKFNKMNLRN 1090 1100 1110 1120 LCIVFSPTLN IPISMLQPFI TDFACIFQGG EPVKEEEREK VDIHIPQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3." Zheng Y., Cerione R., Bender A. J. Biol. Chem. 269:2369-2372(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain." Zheng Y., Hart M.J., Shinjo K., Evans T., Bender A., Cerione R.A. J. Biol. Chem. 268:24629-24634(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194 AND SER-324, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324; SER-423 AND SER-488, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14558 Genomic DNA. Translation: AAA34453.1. U43503 Genomic DNA. Translation: AAB68247.1. BK006949 Genomic DNA. Translation: DAA11319.1.
PIR	A49960.
RefSeq	NP_015210.1. NM_001183929.1.
3D structure databases
ProteinModelPortal	P32873.
SMR	P32873. Positions 633-738, 900-1089.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2575N.
IntAct	P32873. 8 interactions.
MINT	MINT-423650.
STRING	4932.YPL115C.
Proteomic databases
PaxDb	P32873.
PeptideAtlas	P32873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL115C; YPL115C; YPL115C.
GeneID	855988.
KEGG	sce:YPL115C.
Organism-specific databases
CYGD	YPL115c.
SGD	S000006036. BEM3.
Phylogenomic databases
eggNOG	NOG295787.
GeneTree	ENSGT00700000105843.
HOGENOM	HOG000095196.
OMA	PLFVQPE.
OrthoDB	EOG4DNJDC.
Gene expression databases
Genevestigator	P32873.
GermOnline	YPL115C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.555.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001683. Phox. IPR001849. Pleckstrin_homology. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF00787. PX. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00312. PX. 1 hit. SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980837.

Entry information

Entry name

BEM3_YEAST

Accession

Primary (citable) accession number: P32873
Secondary accession number(s): D6W3Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program