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Reviewed, UniProtKB/Swiss-Prot P32871 (PK3CA_BOVIN)

Last modified November 24, 2009. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
    EC=2.7.1.153
Alternative name(s):
    PI3-kinase p110 subunit alpha
    PtdIns-3-kinase p110
      Short name=PI3K
Gene names
Name: PIK3CA
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length1068 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.

Subunit structure

Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 By similarity.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 domain.

Contains 1 PI3K/PI4K domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

PIK3R1P237272EBI-1373130,EBI-520244
PIK3R1P279861EBI-1373130,EBI-79464From a different organism.
PIK3R2P237262EBI-1373130,EBI-1555978

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10681068Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
PRO_0000088784

Regions

Domain319 – 428110C2
Domain797 – 1068272PI3K/PI4K

Secondary structure

1 .................... 1068
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P32871-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C753DCC2F39FDDF0

FASTA1,068124,328
        10         20         30         40         50         60 
MPPRPSSGEL WGIHLMPPRI LVECLLPNGM IVTLECLREA TLITIKHELF KEARKYPLHQ 

        70         80         90        100        110        120 
LLQDESSYIF VSVTQEAERE EFFDETRRLC DLRLFQPFLK VIEPVGNREE KILNREIGFA 

       130        140        150        160        170        180 
IGMPVCEFDM VKDPEVQDFR RNILNVCKEA VDLRDLNSPH SRAMYVYPPN VESSPELPKH 

       190        200        210        220        230        240 
IYNKLDKGQI IVVIWVIVSP NNDKQKYTLK INHDCVPEQV IAEAIRKKTR SMLLSSEQLK 

       250        260        270        280        290        300 
LCVLEYQGKY ILKVCGCDEY FLEKYPLSQY KYIRSCIMLG RMPNLMLMAK ESLYSQLPMD 

       310        320        330        340        350        360 
CFTMPSYSRR ISTATPYMNG ETSTKSLWVI NSALRIKILC ATYVNVNIRD IDKIYVRTGI 

       370        380        390        400        410        420 
YHGGEPLCDN VNTQRVPCSN PRWNEWLNYD IYIPDLPRAA RLCLSICSVK GRKGAKEEHC 

       430        440        450        460        470        480 
PLAWGNINLF DYTDTLVSGK MALNLWPVPH GLEDLLNPIG VTGSNPNKET PCLELEFDWF 

       490        500        510        520        530        540 
SSVVKFPDMS VIEEHANWSV SREAGFSYSH AGLSNRLARD NELRENDKEQ LRAICTRDPL 

       550        560        570        580        590        600 
SEITEQEKDF LWSHRHYCVT IPEILPKLLL SVKWNSRDEV AQMYCLVKDW PPIKPEQAME 

       610        620        630        640        650        660 
LLDCNYPDPM VRGFAVRCLE KYLTDDKLSQ YLIQLVQVLK YEQYLDNLLV RFLLKKALTN 

       670        680        690        700        710        720 
QRIGHFFFWH LKSEMHNKTV SQRFGLLLES YCRACGMYLK HLNRQVEAME KLINLTDILK 

       730        740        750        760        770        780 
QEKKDETQKV QMKFLVEQMR RPDFMDALQG FLSPLNPAHQ LGNLRLEECR IMSSAKRPLW 

       790        800        810        820        830        840 
LNWENPDIMS ELLFQNNEII FKNGDDLRQD MLTLQIIRIM ENIWQNQGLD LRMLPYGCLS 

       850        860        870        880        890        900 
IGDCVGLIEV VRNSHTIMQI QCKGGLKGAL QFNSHTLHQW LKDKNKGEIY DAAIDLFTRS 

       910        920        930        940        950        960 
CAGYCVATFI LGIGDRHNSN IMVKDDGQLF HIDFGHFLDH KKKKFGYKRE RVPFVLTQDF 

       970        980        990       1000       1010       1020 
LIVISKGAQE CTKTREFERF QEMCYKAYLA IRQHANLFIN LFSMMLGSGM PELQSFDDIA 

      1030       1040       1050       1060 
YIRKTLALDK TEQEALEYFM KQMNDAHHGG WTTKMDWIFH TIKQHALN

« Hide

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References

[1]"Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit."
Hiles I.D., Otsu M., Volinia S., Fry M.J., Gout I., Dhand R., Panayotou G., Ruiz-Larrea F., Thompson A., Totty N.F., Hsuan J.J., Courtneidge S.A., Parker P.J., Waterfield M.D.
Cell 70:419-429(1992) [PubMed: 1322797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M93252 mRNA. Translation: AAA30698.1.
IPIIPI00686923.
PIRA43322.
RefSeqNP_776999.1.
UniGeneBt.447

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2V1YX-ray2.40A1-108[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP32871. 4 interactions.
STRINGP32871.

Proteomic databases

PRIDEP32871.

Genome annotation databases

EnsemblENSBTAT00000012168; ENSBTAP00000012168; ENSBTAG00000009232; Bos taurus. [Genome view]
GeneID282306.
KEGGbta:282306.

Organism-specific databases

CTD282306.

Phylogenomic databases

HOVERGENP32871.

Enzyme and pathway databases

BRENDA2.7.1.137. 251.
2.7.1.153. 251.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_reg_CaLB.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2.
IPR003113. PI3K_p85_bd.
IPR000341. PI3K_ras_bd.
IPR001263. PI3Ka.
IPR015433. PI_Kinase.
[Graphical view]
Gene3DG3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHERPTHR10048. PI_Kinase. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF02192. PI3K_p85B. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00143. PI3K_p85B. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
PROSITEPS50004. C2. False negative.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

BindingDBP32871.

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Entry information

Entry namePK3CA_BOVIN
AccessionPrimary (citable) accession number: P32871
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 24, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents