Reviewed, UniProtKB/Swiss-Prot P32870 (CYA1_DROME)

Names and origin

Protein names

Recommended name:
    Ca(2+)/calmodulin-responsive adenylate cyclase
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase
    Protein rutabaga

Gene names

Name:	rut
ORF Names:	CG9533

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a membrane-bound, calmodulin-sensitive adenylyl cyclase. Inactivation of this cyclase leads to a learning and memory defect.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Activated by calcium/calmodulin and G protein.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Mushroom bodies of the fly brain.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Traceable author statement. Source: FlyBase axon extension Inferred from mutant phenotype. Source: FlyBase behavioral response to ethanol Inferred from mutant phenotype. Source: FlyBase cAMP biosynthetic process Traceable author statement. Source: FlyBase cAMP-mediated signaling Non-traceable author statement. Source: FlyBase courtship behavior Traceable author statement. Source: FlyBase determination of adult lifespan Inferred from mutant phenotype. Source: FlyBase grooming behavior Non-traceable author statement. Source: FlyBase memory Inferred from mutant phenotype. Source: FlyBase molting cycle, chitin-based cuticle Inferred from genetic interaction. Source: FlyBase neuromuscular synaptic transmission Inferred from mutant phenotype. Source: FlyBase olfactory learning Traceable author statement. Source: FlyBase response to heat Inferred from mutant phenotype. Source: FlyBase response to oxidative stress Inferred from mutant phenotype. Source: FlyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium- and calmodulin-responsive adenylate cyclase activity Traceable author statement. Source: FlyBase calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2248	2248	Ca(2+)/calmodulin-responsive adenylate cyclase		PRO_0000195716
Regions
	Topological domain	1 – 41	41	Cytoplasmic Potential
	Transmembrane	42 – 60	19	Potential
	Transmembrane	65 – 84	20	Potential
	Transmembrane	101 – 115	15	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	152 – 174	23	Potential
	Transmembrane	186 – 206	21	Potential
	Topological domain	207 – 705	499	Cytoplasmic Potential
	Transmembrane	706 – 726	21	Potential
	Transmembrane	730 – 750	21	Potential
	Transmembrane	770 – 791	22	Potential
	Topological domain	792 – 813	22	Extracellular Potential
	Transmembrane	814 – 834	21	Potential
	Transmembrane	842 – 867	26	Potential
	Transmembrane	868 – 888	21	Potential
	Topological domain	889 – 2248	1360	Cytoplasmic Potential
	Domain	275 – 402	128	Guanylate cyclase 1
	Domain	963 – 1107	145	Guanylate cyclase 2
	Compositional bias	515 – 530	16	Gly-rich
	Compositional bias	569 – 602	34	Gly-rich
	Compositional bias	1278 – 1297	20	Gln-rich
	Compositional bias	1767 – 1810	44	Gly/Ser-rich
	Compositional bias	2025 – 2040	16	Asp/Glu-rich (acidic)
	Compositional bias	2200 – 2241	42	Gln-rich
Sites
	Metal binding	280	1	Magnesium 1 By similarity
	Metal binding	280	1	Magnesium 2 By similarity
	Metal binding	281	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	324	1	Magnesium 1 By similarity
	Metal binding	324	1	Magnesium 2 By similarity
Amino acid modifications
	Glycosylation	800	1	N-linked (GlcNAc...) Potential
	Glycosylation	807	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	1026	1	G → R: Abolishes catalytic activity.
	Sequence conflict	64	1	A → V in AAA28844. Ref.1
	Sequence conflict	596	1	G → S in AAA28844. Ref.1
	Sequence conflict	1286 – 1287	2	QH → HQ Ref.1
	Sequence conflict	1291	1	Q → QH Ref.1
	Sequence conflict	1406	1	S → G in AAA28844. Ref.1
	Sequence conflict	1507	1	Q → H in AAA28844. Ref.1
	Sequence conflict	1511 – 1514	4	HQQP → QPQS in AAA28844. Ref.1
	Sequence conflict	1682	1	H → Q in AAA28844. Ref.1
	Sequence conflict	1696	1	A → T in AAA28844. Ref.1
	Sequence conflict	1771 – 1772	2	Missing in AAA28844. Ref.1
	Sequence conflict	1801	1	S → G in AAA28844. Ref.1
	Sequence conflict	1912	1	H → HH in AAA28844. Ref.1
	Sequence conflict	1950	1	H → N in AAA28844. Ref.1
	Sequence conflict	2218	1	H → Q in AAA28844. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32870-1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: F7E6B4656A2D073C	FASTA	2,248	248,824
	10 20 30 40 50 60 MDHAVKATRG RPLNTLRFEN DELECLYQRY TLKLQRFSVL GVVALVFVLC GVMAALSLTF 70 80 90 100 110 120 NNAATFHNIF NAIVCGLFAV VLVLLQCSVI KDHHLPTLCY GILLFTASIC VVSMPTLGSV 130 140 150 160 170 180 FPVDTKEVMA EGVWQIVFVV FLAYAMMPLQ IWEAVAFGIA LPSVHISLTV YKIFTDALRY 190 200 210 220 230 240 LEYNQLIANI VIFIGVNVAG LVVNIMMERA QRRTFLDTRN CIASRLEIQD ENEKLERLLL 250 260 270 280 290 300 SVLPQHVAMQ MKNDILSPVA GQFHRIYIQK HENVSILFAD IVGFTVLSSQ CSAQELVRLL 310 320 330 340 350 360 NELFGRFDQL AHDNHCLRIK ILGDCYYCVS GLPEPRKDHA KCAVEMGLDM IDAIATVVEA 370 380 390 400 410 420 TDVILNMRVG IHTGRVLCGV LGLRKWQFDV WSNDVTLANH MESGGEPGRV HVTRATLDSL 430 440 450 460 470 480 SGEYEVEAGH GDERSSYLRD HGVDTFFIVP PPHRRKPLML NTLGVRSAIG SRRKLSFRNV 490 500 510 520 530 540 SNVVMQLLHT IKFSEPVPFS NIATGSFPSA ASALGGGVSV GGGGGGGGGG VARGSTCEAN 550 560 570 580 590 600 SGNVQVSEKG SRKVIRLQKI LHATPPPHGM GYGSVVGSGG GVDSGISGGG GCVSGGIAGG 610 620 630 640 650 660 GGVQVTVGTN PNSTASTISR IHRHNHKNNK SQSKVADKFK RPFRKRHSVA AHHQPTNRVN 670 680 690 700 710 720 RFLSQAINAR SVDCDKSEHV DRLTLRFRQS DMEREYHKDF DLGFTTAMGC SLLLLILGAA 730 740 750 760 770 780 LQVTALPRTL ILLLLFLFAF IWVSAILMLL LAVRLKWIIW DISESFSLRM AITIFTVILI 790 800 810 820 830 840 YSVGQVNVFT CVSDHPCSGN GTTSFQNDSH RKCSLPQYVS LSAAFAFLSV SVFLRLPIIF 850 860 870 880 890 900 KSLLVLGMGT IYGLFIELSH QNIFECYDNR VNASIPLHLI SLARIAIFMI AILVHGRLVE 910 920 930 940 950 960 GTARLDFLWQ LQASQEKKEM DVLQESNKRI LHNLLPAHVA AHFLDAQFRN NMELYHQSYA 970 980 990 1000 1010 1020 KVGVIFASVP NFNEFYTEMD GSDQGLECLR LLNEIIADFD ELLKEDRFRG IDKIKTVGST 1030 1040 1050 1060 1070 1080 YMAVVGLIPE YKIQPNDPNS VRRHMTALIE YVKAMRHSLQ EINSHSYNNF MLRVGINIGP 1090 1100 1110 1120 1130 1140 VVAGVIGARK PQYDIWGNTV NVASRMDSTG VPGYSQVTQE VVDSLVGSHF EFRCRGTIKV 1150 1160 1170 1180 1190 1200 KGKGDMVTYF LCDSGNKSLN GEVRNAMSLP QSLHAPDYYM KVSQFPENRV NTDTYSKKEN 1210 1220 1230 1240 1250 1260 GHLYAGNGVE EQQLLLQHQH KQHDPLPLPA PPPPVHHHLH QQQQQRLNSK LQKQPIFMAN 1270 1280 1290 1300 1310 1320 GGLPNIRENG NGHNGEHQQQ QQQQQQHQQQ QQQQQQHGGF MVATTTPPAA VAVPLQPQHH 1330 1340 1350 1360 1370 1380 QLQFQHPHQH PLPSAVSVPV QHQILLHHQL QLQHQPVPSV MLREFNIIEN PTSGGRHQQM 1390 1400 1410 1420 1430 1440 EQLPPHHGSL DLSGMGMGVG AGVLGSDCFM MPRRDRERTY VPPLNQHGHH PPHHLHSNLN 1450 1460 1470 1480 1490 1500 LNQSQHPPSF TSLGYGQCRE SEPLLHASSV APVAKIMPMQ HAPKYEPPRY TSPHTMLSQQ 1510 1520 1530 1540 1550 1560 HQQQQQQQHQ HQQPQSQSAQ DQQTHPAQDP HPLQRQYAMY SQQPQLPPKP VLRTYMKPLP 1570 1580 1590 1600 1610 1620 KLPTDLEESR DMSSTDDLSS RPHSPSMSSS DESYSKTTEG EGEGDEDSPR MVNGGHLHHR 1630 1640 1650 1660 1670 1680 NGYHLPAGGL VNPLQWLYPC DIQVDPTSPV VDMAHLHDFE LSSTTESQGH HTNSNTTSNT 1690 1700 1710 1720 1730 1740 QHKGDSCNSF DFQKAAVGTA AGAAIATKSP FERELQRLLN ESSRARCLAT ATTTAGAIST 1750 1760 1770 1780 1790 1800 TDQTASNGSR ELSYSLSNGK LSSANGHGVG GSGSGSGSGS GSGSAVGNGS GGSGSSNGNL 1810 1820 1830 1840 1850 1860 SGGSGSNSNS GNNNSSHHKT EQQQNMDHEH LAGGKLLGSN SFMIAKHPVG LEAIKEITRN 1870 1880 1890 1900 1910 1920 KNPSESSQMQ TSDTESCEIL HENRNQMHVL AMLEMHTAKE LNGSHAHHGQ HHQQPQRTHR 1930 1940 1950 1960 1970 1980 QRPRSKELQY SHESLDGLDG AVQSQSQQRH QRYHHHHHHQ QRQQQQQRYN HVQEQEERDD 1990 2000 2010 2020 2030 2040 TEDNLADEEF EDDEVGRDVR QKRLQKSELN HKRSEVATEA GNHHDDEVEE EDDDDDEEED 2050 2060 2070 2080 2090 2100 HRNGGREAAP LTNGSMRGLE ANVINDELKY GATHLNHQSM DSNPLESQSE WSDDDCREEA 2110 2120 2130 2140 2150 2160 TGGAESTGYI TDEPGLENIS LLNEAGLTDA EGALSDVNSL YNAPDVDDTS VSSRASSRLL 2170 2180 2190 2200 2210 2220 SLDSLSGLYD CDLDSKHELA IVNASHKISS KFGQPLSPAQ QQHQQQQQQQ QQQQQQHHQQ 2230 2240 QLQQNPQHTQ AQSHLAPVQF QSAEELRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila learning and memory gene rutabaga encodes a Ca2+/Calmodulin-responsive adenylyl cyclase." Levin L.R., Han P.-L., Hwang P.M., Feinstein P.G., Davis R.L., Reed R.R. Cell 68:479-489(1992) [PubMed: 1739965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Canton-S. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81887 mRNA. Translation: AAA28844.1. AE014298 Genomic DNA. Translation: AAF48388.1.
PIR	C42088. D42088.
RefSeq	NP_511156.2.
UniGene	Dm.2613
3D structure databases
HSSP	HSSP built from PDB template 1AZS based on UniProtKB P30803.
SMR	P32870. Positions 263-449.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32870. 1 interaction.
STRING	P32870.
Genome annotation databases
Ensembl	FBtr0073992; FBpp0073809; FBgn0003301; Drosophila melanogaster. [Genome view]
GeneID	32406.
KEGG	dme:Dmel_CG9533.
NMPDR	fig|7227.3.peg.17847.
UCSC	CG9533-RA. d. melanogaster.
Organism-specific databases
CTD	32406.
FlyBase	FBgn0003301. rut.
Phylogenomic databases
HOGENOM	P32870.
OMA	YEVEAGH.
Enzyme and pathway databases
BRENDA	4.6.1.1. 48.
Gene expression databases
Bgee	P32870.
GermOnline	CG9533. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR009398. Aden_cycl-like. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF06327. DUF1053. 1 hit. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 2 hits. [Graphical view]
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 2 hits. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	778293.

Entry information

Entry name

CYA1_DROME

Accession

Primary (citable) accession number: P32870
Secondary accession number(s): Q9TWA7, Q9VY17

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project