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Protein

G protein-coupled receptor kinase 1

Gene

Gprk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei318Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: FlyBase
  • G-protein coupled receptor binding Source: FlyBase
  • G-protein coupled receptor kinase activity Source: FlyBase
  • phosphatidylinositol binding Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • rhodopsin kinase activity Source: FlyBase

GO - Biological processi

  • imaginal disc-derived wing vein specification Source: FlyBase
  • metarhodopsin inactivation Source: FlyBase
  • phototransduction, visible light Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • signal transduction Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14 1994
ReactomeiR-DME-111933 Calmodulin induced events
R-DME-416476 G alpha (q) signalling events
R-DME-418555 G alpha (s) signalling events
R-DME-5635838 Activation of SMO
SignaLinkiP32865

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 1 (EC:2.7.11.16)
Gene namesi
Name:Gprk1
Synonyms:Gprk-1
ORF Names:CG40129
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0260798 Gprk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859651 – 700G protein-coupled receptor kinase 1Add BLAST700

Proteomic databases

PaxDbiP32865
PRIDEiP32865

Expressioni

Gene expression databases

BgeeiFBgn0260798
ExpressionAtlasiP32865 differential
GenevisibleiP32865 DM

Interactioni

GO - Molecular functioni

  • G-protein coupled receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi78138, 1 interactor
IntActiP32865, 1 interactor
STRINGi7227.FBpp0110413

Structurei

3D structure databases

ProteinModelPortaliP32865
SMRiP32865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 175RGSPROSITE-ProRule annotationAdd BLAST122
Domaini191 – 454Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini455 – 522AGC-kinase C-terminalAdd BLAST68
Domaini557 – 657PHPROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190N-terminalAdd BLAST190

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
InParanoidiP32865
KOiK00910
OMAiPLCHRNS
OrthoDBiEOG091G0AEI
PhylomeDBiP32865

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequencei

Sequence statusi: Complete.

P32865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE
60 70 80 90 100
GELNFHKIFN EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE
110 120 130 140 150
RKKMARDIYD NFIMEEMLSH TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL
160 170 180 190 200
EEIFTQLKGK PFKKFLESDK FTRFCQWKNL ELNIQLTMND FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER NMLQAVSTGI
260 270 280 290 300
DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA
310 320 330 340 350
EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA
360 370 380 390 400
SVGTHGYMAP EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK
410 420 430 440 450
LEIDKMTLTM NVELPESFSL ELKNLLEMLL QRDVSKRLGC MGNGADEVKM
460 470 480 490 500
HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA ADAFDIGSFD EEDTKGIKLN
510 520 530 540 550
DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK RKLKQKQHFD
560 570 580 590 600
ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL
610 620 630 640 650
IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS
660 670 680 690 700
SLRSAHKISQ DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN
Length:700
Mass (Da):80,566
Last modified:April 14, 2009 - v2
Checksum:i44C6F3988A6A91CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58I → N in AAA28588 (PubMed:1662381).Curated1
Sequence conflicti620N → I in ACH92540 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80493 mRNA Translation: AAA28588.1
AE013599 Genomic DNA Translation: EAA46156.2
BT022770 mRNA Translation: AAY55186.1
BT022801 mRNA Translation: AAY55217.1
BT044475 mRNA Translation: ACH92540.1
PIRiA41615
RefSeqiNP_001036438.1, NM_001042973.3
NP_001163051.1, NM_001169580.2
NP_001286135.1, NM_001299206.1
UniGeneiDm.1704

Genome annotation databases

EnsemblMetazoaiFBtr0111121; FBpp0110413; FBgn0260798
FBtr0302242; FBpp0291451; FBgn0260798
FBtr0345363; FBpp0311517; FBgn0260798
GeneIDi3355013
KEGGidme:Dmel_CG40129

Similar proteinsi

Entry informationi

Entry nameiGPRK1_DROME
AccessioniPrimary (citable) accession number: P32865
Secondary accession number(s): B5RJK1, Q4V555, Q7PLS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 14, 2009
Last modified: March 28, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health