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P32865 (GPRK1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 1

EC=2.7.11.16
Gene names
Name:Gprk1
Synonyms:Gprk-1
ORF Names:CG40129
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length700 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors By similarity.

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 700700G protein-coupled receptor kinase 1
PRO_0000085965

Regions

Domain54 – 175122RGS
Domain191 – 454264Protein kinase
Domain455 – 52268AGC-kinase C-terminal
Domain557 – 657101PH
Nucleotide binding197 – 2059ATP By similarity
Region1 – 190190N-terminal

Sites

Active site3181Proton acceptor By similarity
Binding site2201ATP By similarity

Experimental info

Sequence conflict581I → N in AAA28588. Ref.1
Sequence conflict6201N → I in ACH92540. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P32865 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 44C6F3988A6A91CF

FASTA70080,566
        10         20         30         40         50         60 
MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE GELNFHKIFN 

        70         80         90        100        110        120 
EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE RKKMARDIYD NFIMEEMLSH 

       130        140        150        160        170        180 
TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL EEIFTQLKGK PFKKFLESDK FTRFCQWKNL 

       190        200        210        220        230        240 
ELNIQLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER 

       250        260        270        280        290        300 
NMLQAVSTGI DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA 

       310        320        330        340        350        360 
EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA SVGTHGYMAP 

       370        380        390        400        410        420 
EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK LEIDKMTLTM NVELPESFSL 

       430        440        450        460        470        480 
ELKNLLEMLL QRDVSKRLGC MGNGADEVKM HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA 

       490        500        510        520        530        540 
ADAFDIGSFD EEDTKGIKLN DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK 

       550        560        570        580        590        600 
RKLKQKQHFD ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL 

       610        620        630        640        650        660 
IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS SLRSAHKISQ 

       670        680        690        700 
DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of Drosophila genes encoding G protein-coupled receptor kinases."
Cassill J.A., Whitney M., Joazeiro C.A., Becker A., Zuker C.S.
Proc. Natl. Acad. Sci. U.S.A. 88:11067-11070(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80493 mRNA. Translation: AAA28588.1.
AE013599 Genomic DNA. Translation: EAA46156.2.
BT022770 mRNA. Translation: AAY55186.1.
BT022801 mRNA. Translation: AAY55217.1.
BT044475 mRNA. Translation: ACH92540.1.
PIRA41615.
RefSeqNP_001036438.1. NM_001042973.3.
NP_001163051.1. NM_001169580.2.
UniGeneDm.1704.

3D structure databases

ProteinModelPortalP32865.
SMRP32865. Positions 30-672.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid78138. 1 interaction.
IntActP32865. 1 interaction.
MINTMINT-1700409.

Proteomic databases

PaxDbP32865.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0111121; FBpp0110413; FBgn0260798.
FBtr0302242; FBpp0291451; FBgn0260798.
GeneID3355013.
KEGGdme:Dmel_CG40129.

Organism-specific databases

CTD3355013.
FlyBaseFBgn0260798. Gprk1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110751.
InParanoidP32865.
KOK00910.
OMAKGEQCIV.
OrthoDBEOG7FV3PP.
PhylomeDBP32865.

Enzyme and pathway databases

BRENDA2.7.11.14. 1994.
SignaLinkP32865.

Gene expression databases

BgeeP32865.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi3355013.
NextBio850121.
PROP32865.

Entry information

Entry nameGPRK1_DROME
AccessionPrimary (citable) accession number: P32865
Secondary accession number(s): B5RJK1, Q4V555, Q7PLS9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 14, 2009
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase