Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32865

- GPRK1_DROME

UniProt

P32865 - GPRK1_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

G protein-coupled receptor kinase 1

Gene

Gprk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPPROSITE-ProRule annotation
Active sitei318 – 3181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: FlyBase
  2. G-protein coupled receptor binding Source: FlyBase
  3. G-protein coupled receptor kinase activity Source: FlyBase
  4. phosphatidylinositol binding Source: FlyBase
  5. protein serine/threonine kinase activity Source: FlyBase
  6. rhodopsin kinase activity Source: FlyBase

GO - Biological processi

  1. imaginal disc-derived wing vein specification Source: FlyBase
  2. metarhodopsin inactivation Source: FlyBase
  3. phototransduction, visible light Source: FlyBase
  4. protein phosphorylation Source: FlyBase
  5. signal transduction Source: FlyBase
  6. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14. 1994.
ReactomeiREACT_209150. Calmodulin induced events.
SignaLinkiP32865.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 1 (EC:2.7.11.16)
Gene namesi
Name:Gprk1
Synonyms:Gprk-1
ORF Names:CG40129
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0260798. Gprk1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700G protein-coupled receptor kinase 1PRO_0000085965Add
BLAST

Proteomic databases

PaxDbiP32865.

Expressioni

Gene expression databases

BgeeiP32865.

Interactioni

Protein-protein interaction databases

BioGridi78138. 1 interaction.
IntActiP32865. 1 interaction.
MINTiMINT-1700409.

Structurei

3D structure databases

ProteinModelPortaliP32865.
SMRiP32865. Positions 30-672.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
BLAST
Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini455 – 52268AGC-kinase C-terminalAdd
BLAST
Domaini557 – 657101PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190N-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118793.
InParanoidiP32865.
KOiK00910.
OMAiKGEQCIV.
OrthoDBiEOG7FV3PP.
PhylomeDBiP32865.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32865-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE
60 70 80 90 100
GELNFHKIFN EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE
110 120 130 140 150
RKKMARDIYD NFIMEEMLSH TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL
160 170 180 190 200
EEIFTQLKGK PFKKFLESDK FTRFCQWKNL ELNIQLTMND FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER NMLQAVSTGI
260 270 280 290 300
DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA
310 320 330 340 350
EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA
360 370 380 390 400
SVGTHGYMAP EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK
410 420 430 440 450
LEIDKMTLTM NVELPESFSL ELKNLLEMLL QRDVSKRLGC MGNGADEVKM
460 470 480 490 500
HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA ADAFDIGSFD EEDTKGIKLN
510 520 530 540 550
DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK RKLKQKQHFD
560 570 580 590 600
ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL
610 620 630 640 650
IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS
660 670 680 690 700
SLRSAHKISQ DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN
Length:700
Mass (Da):80,566
Last modified:April 14, 2009 - v2
Checksum:i44C6F3988A6A91CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581I → N in AAA28588. (PubMed:1662381)Curated
Sequence conflicti620 – 6201N → I in ACH92540. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80493 mRNA. Translation: AAA28588.1.
AE013599 Genomic DNA. Translation: EAA46156.2.
BT022770 mRNA. Translation: AAY55186.1.
BT022801 mRNA. Translation: AAY55217.1.
BT044475 mRNA. Translation: ACH92540.1.
PIRiA41615.
RefSeqiNP_001036438.1. NM_001042973.3.
NP_001163051.1. NM_001169580.2.
UniGeneiDm.1704.

Genome annotation databases

EnsemblMetazoaiFBtr0111121; FBpp0110413; FBgn0260798.
FBtr0302242; FBpp0291451; FBgn0260798.
GeneIDi3355013.
KEGGidme:Dmel_CG40129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80493 mRNA. Translation: AAA28588.1 .
AE013599 Genomic DNA. Translation: EAA46156.2 .
BT022770 mRNA. Translation: AAY55186.1 .
BT022801 mRNA. Translation: AAY55217.1 .
BT044475 mRNA. Translation: ACH92540.1 .
PIRi A41615.
RefSeqi NP_001036438.1. NM_001042973.3.
NP_001163051.1. NM_001169580.2.
UniGenei Dm.1704.

3D structure databases

ProteinModelPortali P32865.
SMRi P32865. Positions 30-672.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 78138. 1 interaction.
IntActi P32865. 1 interaction.
MINTi MINT-1700409.

Proteomic databases

PaxDbi P32865.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0111121 ; FBpp0110413 ; FBgn0260798 .
FBtr0302242 ; FBpp0291451 ; FBgn0260798 .
GeneIDi 3355013.
KEGGi dme:Dmel_CG40129.

Organism-specific databases

CTDi 3355013.
FlyBasei FBgn0260798. Gprk1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118793.
InParanoidi P32865.
KOi K00910.
OMAi KGEQCIV.
OrthoDBi EOG7FV3PP.
PhylomeDBi P32865.

Enzyme and pathway databases

BRENDAi 2.7.11.14. 1994.
Reactomei REACT_209150. Calmodulin induced events.
SignaLinki P32865.

Miscellaneous databases

GenomeRNAii 3355013.
NextBioi 850121.
PROi P32865.

Gene expression databases

Bgeei P32865.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of Drosophila genes encoding G protein-coupled receptor kinases."
    Cassill J.A., Whitney M., Joazeiro C.A., Becker A., Zuker C.S.
    Proc. Natl. Acad. Sci. U.S.A. 88:11067-11070(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiGPRK1_DROME
AccessioniPrimary (citable) accession number: P32865
Secondary accession number(s): B5RJK1, Q4V555, Q7PLS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 14, 2009
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3