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P32865

- GPRK1_DROME

UniProt

P32865 - GPRK1_DROME

Protein

G protein-coupled receptor kinase 1

Gene

Gprk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Specifically phosphorylates the activated forms of G protein-coupled receptors.By similarity

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei318 – 3181Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: FlyBase
    2. G-protein coupled receptor binding Source: FlyBase
    3. G-protein coupled receptor kinase activity Source: FlyBase
    4. phosphatidylinositol binding Source: FlyBase
    5. protein serine/threonine kinase activity Source: FlyBase
    6. rhodopsin kinase activity Source: FlyBase

    GO - Biological processi

    1. imaginal disc-derived wing vein specification Source: FlyBase
    2. metarhodopsin inactivation Source: FlyBase
    3. phototransduction, visible light Source: FlyBase
    4. protein phosphorylation Source: FlyBase
    5. signal transduction Source: FlyBase
    6. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.14. 1994.
    ReactomeiREACT_209150. Calmodulin induced events.
    SignaLinkiP32865.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 1 (EC:2.7.11.16)
    Gene namesi
    Name:Gprk1
    Synonyms:Gprk-1
    ORF Names:CG40129
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0260798. Gprk1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 700700G protein-coupled receptor kinase 1PRO_0000085965Add
    BLAST

    Proteomic databases

    PaxDbiP32865.

    Expressioni

    Gene expression databases

    BgeeiP32865.

    Interactioni

    Protein-protein interaction databases

    BioGridi78138. 1 interaction.
    IntActiP32865. 1 interaction.
    MINTiMINT-1700409.

    Structurei

    3D structure databases

    ProteinModelPortaliP32865.
    SMRiP32865. Positions 30-672.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini455 – 52268AGC-kinase C-terminalAdd
    BLAST
    Domaini557 – 657101PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190N-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110751.
    InParanoidiP32865.
    KOiK00910.
    OMAiKGEQCIV.
    OrthoDBiEOG7FV3PP.
    PhylomeDBiP32865.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32865-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE    50
    GELNFHKIFN EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE 100
    RKKMARDIYD NFIMEEMLSH TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL 150
    EEIFTQLKGK PFKKFLESDK FTRFCQWKNL ELNIQLTMND FSVHRIIGRG 200
    GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER NMLQAVSTGI 250
    DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA 300
    EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA 350
    SVGTHGYMAP EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK 400
    LEIDKMTLTM NVELPESFSL ELKNLLEMLL QRDVSKRLGC MGNGADEVKM 450
    HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA ADAFDIGSFD EEDTKGIKLN 500
    DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK RKLKQKQHFD 550
    ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL 600
    IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS 650
    SLRSAHKISQ DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN 700
    Length:700
    Mass (Da):80,566
    Last modified:April 14, 2009 - v2
    Checksum:i44C6F3988A6A91CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581I → N in AAA28588. (PubMed:1662381)Curated
    Sequence conflicti620 – 6201N → I in ACH92540. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80493 mRNA. Translation: AAA28588.1.
    AE013599 Genomic DNA. Translation: EAA46156.2.
    BT022770 mRNA. Translation: AAY55186.1.
    BT022801 mRNA. Translation: AAY55217.1.
    BT044475 mRNA. Translation: ACH92540.1.
    PIRiA41615.
    RefSeqiNP_001036438.1. NM_001042973.3.
    NP_001163051.1. NM_001169580.2.
    UniGeneiDm.1704.

    Genome annotation databases

    EnsemblMetazoaiFBtr0111121; FBpp0110413; FBgn0260798.
    FBtr0302242; FBpp0291451; FBgn0260798.
    GeneIDi3355013.
    KEGGidme:Dmel_CG40129.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80493 mRNA. Translation: AAA28588.1 .
    AE013599 Genomic DNA. Translation: EAA46156.2 .
    BT022770 mRNA. Translation: AAY55186.1 .
    BT022801 mRNA. Translation: AAY55217.1 .
    BT044475 mRNA. Translation: ACH92540.1 .
    PIRi A41615.
    RefSeqi NP_001036438.1. NM_001042973.3.
    NP_001163051.1. NM_001169580.2.
    UniGenei Dm.1704.

    3D structure databases

    ProteinModelPortali P32865.
    SMRi P32865. Positions 30-672.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 78138. 1 interaction.
    IntActi P32865. 1 interaction.
    MINTi MINT-1700409.

    Proteomic databases

    PaxDbi P32865.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0111121 ; FBpp0110413 ; FBgn0260798 .
    FBtr0302242 ; FBpp0291451 ; FBgn0260798 .
    GeneIDi 3355013.
    KEGGi dme:Dmel_CG40129.

    Organism-specific databases

    CTDi 3355013.
    FlyBasei FBgn0260798. Gprk1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110751.
    InParanoidi P32865.
    KOi K00910.
    OMAi KGEQCIV.
    OrthoDBi EOG7FV3PP.
    PhylomeDBi P32865.

    Enzyme and pathway databases

    BRENDAi 2.7.11.14. 1994.
    Reactomei REACT_209150. Calmodulin induced events.
    SignaLinki P32865.

    Miscellaneous databases

    GenomeRNAii 3355013.
    NextBioi 850121.
    PROi P32865.

    Gene expression databases

    Bgeei P32865.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of Drosophila genes encoding G protein-coupled receptor kinases."
      Cassill J.A., Whitney M., Joazeiro C.A., Becker A., Zuker C.S.
      Proc. Natl. Acad. Sci. U.S.A. 88:11067-11070(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiGPRK1_DROME
    AccessioniPrimary (citable) accession number: P32865
    Secondary accession number(s): B5RJK1, Q4V555, Q7PLS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3