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Protein

G protein-coupled receptor kinase 1

Gene

Gprk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei318Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: FlyBase
  • G-protein coupled receptor binding Source: FlyBase
  • G-protein coupled receptor kinase activity Source: FlyBase
  • phosphatidylinositol binding Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • rhodopsin kinase activity Source: FlyBase

GO - Biological processi

  • imaginal disc-derived wing vein specification Source: FlyBase
  • metarhodopsin inactivation Source: FlyBase
  • phototransduction, visible light Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • signal transduction Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14. 1994.
ReactomeiR-DME-111933. Calmodulin induced events.
R-DME-416476. G alpha (q) signalling events.
R-DME-5635838. Activation of SMO.
SignaLinkiP32865.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 1 (EC:2.7.11.16)
Gene namesi
Name:Gprk1
Synonyms:Gprk-1
ORF Names:CG40129
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0260798. Gprk1.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859651 – 700G protein-coupled receptor kinase 1Add BLAST700

Proteomic databases

PaxDbiP32865.
PRIDEiP32865.

Expressioni

Gene expression databases

BgeeiFBgn0260798.
ExpressionAtlasiP32865. differential.
GenevisibleiP32865. DM.

Interactioni

GO - Molecular functioni

  • G-protein coupled receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi78138. 1 interactor.
IntActiP32865. 1 interactor.
MINTiMINT-1700409.
STRINGi7227.FBpp0110413.

Structurei

3D structure databases

ProteinModelPortaliP32865.
SMRiP32865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 175RGSPROSITE-ProRule annotationAdd BLAST122
Domaini191 – 454Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini455 – 522AGC-kinase C-terminalAdd BLAST68
Domaini557 – 657PHPROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190N-terminalAdd BLAST190

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
InParanoidiP32865.
KOiK00910.
OMAiPLCHRNS.
OrthoDBiEOG091G0AEI.
PhylomeDBiP32865.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
PRINTSiPR00717. GPCRKINASE.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.

Sequencei

Sequence statusi: Complete.

P32865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE
60 70 80 90 100
GELNFHKIFN EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE
110 120 130 140 150
RKKMARDIYD NFIMEEMLSH TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL
160 170 180 190 200
EEIFTQLKGK PFKKFLESDK FTRFCQWKNL ELNIQLTMND FSVHRIIGRG
210 220 230 240 250
GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER NMLQAVSTGI
260 270 280 290 300
DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA
310 320 330 340 350
EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA
360 370 380 390 400
SVGTHGYMAP EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK
410 420 430 440 450
LEIDKMTLTM NVELPESFSL ELKNLLEMLL QRDVSKRLGC MGNGADEVKM
460 470 480 490 500
HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA ADAFDIGSFD EEDTKGIKLN
510 520 530 540 550
DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK RKLKQKQHFD
560 570 580 590 600
ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL
610 620 630 640 650
IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS
660 670 680 690 700
SLRSAHKISQ DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN
Length:700
Mass (Da):80,566
Last modified:April 14, 2009 - v2
Checksum:i44C6F3988A6A91CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58I → N in AAA28588 (PubMed:1662381).Curated1
Sequence conflicti620N → I in ACH92540 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80493 mRNA. Translation: AAA28588.1.
AE013599 Genomic DNA. Translation: EAA46156.2.
BT022770 mRNA. Translation: AAY55186.1.
BT022801 mRNA. Translation: AAY55217.1.
BT044475 mRNA. Translation: ACH92540.1.
PIRiA41615.
RefSeqiNP_001036438.1. NM_001042973.3.
NP_001163051.1. NM_001169580.2.
NP_001286135.1. NM_001299206.1.
UniGeneiDm.1704.

Genome annotation databases

EnsemblMetazoaiFBtr0111121; FBpp0110413; FBgn0260798.
FBtr0302242; FBpp0291451; FBgn0260798.
FBtr0345363; FBpp0311517; FBgn0260798.
GeneIDi3355013.
KEGGidme:Dmel_CG40129.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGPRK1_DROME
AccessioniPrimary (citable) accession number: P32865
Secondary accession number(s): B5RJK1, Q4V555, Q7PLS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 14, 2009
Last modified: July 5, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families