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Protein

DNA repair and recombination protein RAD54

Gene

RAD54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi335 – 3428ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • DNA translocase activity Source: SGD
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA geometric change Source: SGD
  • double-strand break repair via synthesis-dependent strand annealing Source: SGD
  • heteroduplex formation Source: SGD
  • positive regulation of endodeoxyribonuclease activity Source: SGD
  • telomere maintenance via recombination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30652-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD54 (EC:3.6.4.-)
Gene namesi
Name:RAD54
Ordered Locus Names:YGL163C
ORF Names:G1821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL163C.
SGDiS000003131. RAD54.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898DNA repair and recombination protein RAD54PRO_0000074344Add
BLAST

PTM databases

iPTMnetiP32863.

Expressioni

Inductioni

Expression increases in late G1 phase compared to other phases of the cell cycle.

Interactioni

Subunit structurei

Interacts with RAD51.1 Publication

Protein-protein interaction databases

BioGridi33090. 192 interactions.
DIPiDIP-509N.
IntActiP32863. 5 interactions.
MINTiMINT-605745.

Structurei

3D structure databases

ProteinModelPortaliP32863.
SMRiP32863. Positions 259-866.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini322 – 504183Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini659 – 812154Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 255Nuclear localization signalSequence analysis
Motifi41 – 455Nuclear localization signalSequence analysis
Motifi455 – 4584DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074619.
HOGENOMiHOG000204521.
InParanoidiP32863.
KOiK10875.
OMAiYLPCKYE.
OrthoDBiEOG7SXWC3.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR013967. Rad54_N.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF08658. Rad54_N. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRRLPDRP PNGIGAGERP RLVPRPINVQ DSVNRLTKPF RVPYKNTHIP
60 70 80 90 100
PAAGRIATGS DNIVGGRSLR KRSATVCYSG LDINADEAEY NSQDISFSQL
110 120 130 140 150
TKRRKDALSA QRLAKDPTRL SHIQYTLRRS FTVPIKGYVQ RHSLPLTLGM
160 170 180 190 200
KKKITPEPRP LHDPTDEFAI VLYDPSVDGE MIVHDTSMDN KEEESKKMIK
210 220 230 240 250
STQEKDNINK EKNSQEERPT QRIGRHPALM TNGVRNKPLR ELLGDSENSA
260 270 280 290 300
ENKKKFASVP VVIDPKLAKI LRPHQVEGVR FLYRCVTGLV MKDYLEAEAF
310 320 330 340 350
NTSSEDPLKS DEKALTESQK TEQNNRGAYG CIMADEMGLG KTLQCIALMW
360 370 380 390 400
TLLRQGPQGK RLIDKCIIVC PSSLVNNWAN ELIKWLGPNT LTPLAVDGKK
410 420 430 440 450
SSMGGGNTTV SQAIHAWAQA QGRNIVKPVL IISYETLRRN VDQLKNCNVG
460 470 480 490 500
LMLADEGHRL KNGDSLTFTA LDSISCPRRV ILSGTPIQND LSEYFALLSF
510 520 530 540 550
SNPGLLGSRA EFRKNFENPI LRGRDADATD KEITKGEAQL QKLSTIVSKF
560 570 580 590 600
IIRRTNDILA KYLPCKYEHV IFVNLKPLQN ELYNKLIKSR EVKKVVKGVG
610 620 630 640 650
GSQPLRAIGI LKKLCNHPNL LNFEDEFDDE DDLELPDDYN MPGSKARDVQ
660 670 680 690 700
TKYSAKFSIL ERFLHKIKTE SDDKIVLISN YTQTLDLIEK MCRYKHYSAV
710 720 730 740 750
RLDGTMSINK RQKLVDRFND PEGQEFIFLL SSKAGGCGIN LIGANRLILM
760 770 780 790 800
DPDWNPAADQ QALARVWRDG QKKDCFIYRF ISTGTIEEKI FQRQSMKMSL
810 820 830 840 850
SSCVVDAKED VERLFSSDNL RQLFQKNENT ICETHETYHC KRCNAQGKQL
860 870 880 890
KRAPAMLYGD ATTWNHLNHD ALEKTNDHLL KNEHHYNDIS FAFQYISH
Length:898
Mass (Da):101,754
Last modified:October 1, 1993 - v1
Checksum:i2EC0A9B7E3D66292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63232 Genomic DNA. Translation: AAA34949.1.
Z48618 Genomic DNA. Translation: CAA88534.1.
Z72685 Genomic DNA. Translation: CAA96875.1.
BK006941 Genomic DNA. Translation: DAA07949.1.
PIRiJH0440.
RefSeqiNP_011352.1. NM_001181028.1.

Genome annotation databases

EnsemblFungiiYGL163C; YGL163C; YGL163C.
GeneIDi852713.
KEGGisce:YGL163C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63232 Genomic DNA. Translation: AAA34949.1.
Z48618 Genomic DNA. Translation: CAA88534.1.
Z72685 Genomic DNA. Translation: CAA96875.1.
BK006941 Genomic DNA. Translation: DAA07949.1.
PIRiJH0440.
RefSeqiNP_011352.1. NM_001181028.1.

3D structure databases

ProteinModelPortaliP32863.
SMRiP32863. Positions 259-866.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33090. 192 interactions.
DIPiDIP-509N.
IntActiP32863. 5 interactions.
MINTiMINT-605745.

PTM databases

iPTMnetiP32863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL163C; YGL163C; YGL163C.
GeneIDi852713.
KEGGisce:YGL163C.

Organism-specific databases

EuPathDBiFungiDB:YGL163C.
SGDiS000003131. RAD54.

Phylogenomic databases

GeneTreeiENSGT00550000074619.
HOGENOMiHOG000204521.
InParanoidiP32863.
KOiK10875.
OMAiYLPCKYE.
OrthoDBiEOG7SXWC3.

Enzyme and pathway databases

BioCyciYEAST:G3O-30652-MONOMER.

Miscellaneous databases

NextBioi972079.
PROiP32863.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR013967. Rad54_N.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF08658. Rad54_N. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair."
    Emery H.S., Schild D., Kellogg D.E., Mortimer R.K.
    Gene 104:103-106(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43."
    James C.M., Indge K.J., Oliver S.G.
    Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The DNA repair genes RAD54 and UNG1 are cell cycle regulated in budding yeast but MCB promoter elements have no essential role in the DNA damage response."
    Johnston L.H., Johnson A.L.
    Nucleic Acids Res. 23:2147-2152(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CELL CYCLE REGULATION.
  6. "Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins."
    Petukhova G., Stratton S., Sung P.
    Nature 393:91-94(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51.
  7. "Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments."
    Solinger J.A., Kiianitsa K., Heyer W.-D.
    Mol. Cell 10:1175-1188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD54_YEAST
AccessioniPrimary (citable) accession number: P32863
Secondary accession number(s): D6VTY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 11, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.