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Protein

DNA repair and recombination protein RAD54

Gene

RAD54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • DNA translocase activity Source: SGD
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA geometric change Source: SGD
  • double-strand break repair via synthesis-dependent strand annealing Source: SGD
  • heteroduplex formation Source: SGD
  • positive regulation of endodeoxyribonuclease activity Source: SGD
  • telomere maintenance via recombination Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30652-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD54 (EC:3.6.4.-)
Gene namesi
Name:RAD54
Ordered Locus Names:YGL163C
ORF Names:G1821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL163C
SGDiS000003131 RAD54

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743441 – 898DNA repair and recombination protein RAD54Add BLAST898

Proteomic databases

PaxDbiP32863
PRIDEiP32863

PTM databases

iPTMnetiP32863

Expressioni

Inductioni

Expression increases in late G1 phase compared to other phases of the cell cycle.

Interactioni

Subunit structurei

Interacts with RAD51.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD51P254542EBI-14728,EBI-14709

Protein-protein interaction databases

BioGridi33090, 295 interactors
DIPiDIP-509N
IntActiP32863, 29 interactors
MINTiP32863
STRINGi4932.YGL163C

Structurei

3D structure databases

ProteinModelPortaliP32863
SMRiP32863
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini322 – 504Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST183
Domaini659 – 812Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 25Nuclear localization signalSequence analysis5
Motifi41 – 45Nuclear localization signalSequence analysis5
Motifi455 – 458DEGH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144164
HOGENOMiHOG000204521
InParanoidiP32863
KOiK10875
OMAiNILRPHQ
OrthoDBiEOG092C10RV

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR013967 Rad54_N
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF08658 Rad54_N, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P32863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRRLPDRP PNGIGAGERP RLVPRPINVQ DSVNRLTKPF RVPYKNTHIP
60 70 80 90 100
PAAGRIATGS DNIVGGRSLR KRSATVCYSG LDINADEAEY NSQDISFSQL
110 120 130 140 150
TKRRKDALSA QRLAKDPTRL SHIQYTLRRS FTVPIKGYVQ RHSLPLTLGM
160 170 180 190 200
KKKITPEPRP LHDPTDEFAI VLYDPSVDGE MIVHDTSMDN KEEESKKMIK
210 220 230 240 250
STQEKDNINK EKNSQEERPT QRIGRHPALM TNGVRNKPLR ELLGDSENSA
260 270 280 290 300
ENKKKFASVP VVIDPKLAKI LRPHQVEGVR FLYRCVTGLV MKDYLEAEAF
310 320 330 340 350
NTSSEDPLKS DEKALTESQK TEQNNRGAYG CIMADEMGLG KTLQCIALMW
360 370 380 390 400
TLLRQGPQGK RLIDKCIIVC PSSLVNNWAN ELIKWLGPNT LTPLAVDGKK
410 420 430 440 450
SSMGGGNTTV SQAIHAWAQA QGRNIVKPVL IISYETLRRN VDQLKNCNVG
460 470 480 490 500
LMLADEGHRL KNGDSLTFTA LDSISCPRRV ILSGTPIQND LSEYFALLSF
510 520 530 540 550
SNPGLLGSRA EFRKNFENPI LRGRDADATD KEITKGEAQL QKLSTIVSKF
560 570 580 590 600
IIRRTNDILA KYLPCKYEHV IFVNLKPLQN ELYNKLIKSR EVKKVVKGVG
610 620 630 640 650
GSQPLRAIGI LKKLCNHPNL LNFEDEFDDE DDLELPDDYN MPGSKARDVQ
660 670 680 690 700
TKYSAKFSIL ERFLHKIKTE SDDKIVLISN YTQTLDLIEK MCRYKHYSAV
710 720 730 740 750
RLDGTMSINK RQKLVDRFND PEGQEFIFLL SSKAGGCGIN LIGANRLILM
760 770 780 790 800
DPDWNPAADQ QALARVWRDG QKKDCFIYRF ISTGTIEEKI FQRQSMKMSL
810 820 830 840 850
SSCVVDAKED VERLFSSDNL RQLFQKNENT ICETHETYHC KRCNAQGKQL
860 870 880 890
KRAPAMLYGD ATTWNHLNHD ALEKTNDHLL KNEHHYNDIS FAFQYISH
Length:898
Mass (Da):101,754
Last modified:October 1, 1993 - v1
Checksum:i2EC0A9B7E3D66292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63232 Genomic DNA Translation: AAA34949.1
Z48618 Genomic DNA Translation: CAA88534.1
Z72685 Genomic DNA Translation: CAA96875.1
BK006941 Genomic DNA Translation: DAA07949.1
PIRiJH0440
RefSeqiNP_011352.1, NM_001181028.1

Genome annotation databases

EnsemblFungiiYGL163C; YGL163C; YGL163C
GeneIDi852713
KEGGisce:YGL163C

Similar proteinsi

Entry informationi

Entry nameiRAD54_YEAST
AccessioniPrimary (citable) accession number: P32863
Secondary accession number(s): D6VTY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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