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Protein

Glucose transport transcription regulator RGT1

Gene

RGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose. In the absence of glucose, it functions as a transcriptional repressor, whereas high concentrations of glucose cause it to function as a transcriptional activator. In cells growing on low levels of glucose, has a neutral role, neither repressing nor activating transcription. Binds the consensus binding site sequence 5'-CGGANNA-3', of which multiple copies are present in all HXT promoters regulated by RGT1.3 Publications

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi47 – 76Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Source: GO_Central
  • regulation of glucose import Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31839-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose transport transcription regulator RGT1
Alternative name(s):
Restores glucose transport protein 1
Gene namesi
Name:RGT1
Ordered Locus Names:YKL038W
ORF Names:YKL251
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL038W
SGDiS000001521 RGT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001150011 – 1170Glucose transport transcription regulator RGT1Add BLAST1170

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei414PhosphoserineCombined sources1
Modified residuei1130PhosphoserineCombined sources1

Post-translational modificationi

Glucose-induced phosphorylation regulates the DNA-binding activity. Hyperphosphorylation in cells growing on high levels of glucose does prevents DNA-binding and dephosphorylation restores DNA-binding ability.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32862
PaxDbiP32862
PRIDEiP32862

PTM databases

iPTMnetiP32862

Interactioni

Protein-protein interaction databases

BioGridi34094, 112 interactors
DIPiDIP-4177N
IntActiP32862, 6 interactors
STRINGi4932.YKL038W

Structurei

3D structure databases

ProteinModelPortaliP32862
SMRiP32862
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EDS1/RGT1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000067048
InParanoidiP32862
KOiK21641
OMAiWFRNSLE
OrthoDBiEOG092C0CBX

Family and domain databases

CDDicd00067 GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNELNTVSTN SSDSTKNGGT SNSPDDMDSA AAASHAIKKR TKASRACDQC
60 70 80 90 100
RKKKIKCDYK DEKGVCSNCQ RNGDRCSFDR VPLKRGPSKG YTRSTSHPRT
110 120 130 140 150
NEIQDHNNSR SYNTFDNSNN TLNNNTGNSG DNGINSNTVP STPSRSNSVL
160 170 180 190 200
LPPLTQYIPQ AGGIPPSFQN PAIQSTMPAG NIGQQQFWKV PYHEFQHQRK
210 220 230 240 250
GSIDSLQSDI SVRTLNPNEQ LSYNTVQQSP ITNKHTNDSG NANGSVTGSG
260 270 280 290 300
SASGSGGYWS FIRTSGLLAP TDDHNGEQTR RSSSIPSLLR NTSNSLLLGG
310 320 330 340 350
QPQLPPPQQQ SQPQAHQQKL QQGQNLYSYS QFSQQQPYNP SISSFGQFAA
360 370 380 390 400
NGFHSRQGSV ASEAMSPSAP AMFTSTSTNP VNVAQQTQRP QGQQVPQFSS
410 420 430 440 450
ELDGNKRRQS APVSVTLSTD RLNGNENNNG EINNNNGSNN SGSSKDTSQH
460 470 480 490 500
SQESVTTPAA LEASSPGSTP QRSTKKRRKS YVSKKTKPKR DSSISITSKD
510 520 530 540 550
SAHPMTTSST IAYGQISDVD LIDTYYEFIH VGFPIIPLNK TTLTSDLLLV
560 570 580 590 600
NTQPISNIHE VNSYVILWFR NSLELLVRVA LKQKPGGKFF DNIVGVALSP
610 620 630 640 650
SNDNNKAGFT TATARDDAEK TRRDSHNEVQ DTLEVQSVFI AALNECFQKI
660 670 680 690 700
VDIHPKFREN NDQISPKIKV IYLSTFILLN YILAFVGYDN SFVLGMSVTI
710 720 730 740 750
FNEFKLYKLL LFPEPDINDV KPPVDEEVST GNGNTKTSEF EIGSESAGHM
760 770 780 790 800
NPSNSPNSMD ENISHYSVLF KRLYVLLSVF DSLQSCAFGG PKLLNISIQG
810 820 830 840 850
STERFFSNDL GSKWCLEQSQ LRLKSVLQSL KLGELMSELT RNRISMNGNR
860 870 880 890 900
KPGFDITNSS SLLSEYVETQ PLSVAQLFCK LLIGKHNFIN CLLSLYDSEA
910 920 930 940 950
GVYSDLTLDL SSKIADSLCS LISIILQVLT LILRLNPTNS IDFNYRPPNP
960 970 980 990 1000
PANNPTVQEG PSAMGSSPVA GNLSAAPPSE GNPDFYKKLL GLKQDTGTIL
1010 1020 1030 1040 1050
SDLCRGIISP FAIAILHEVY NITELVKQMP TSLISIMMTA TTTQNTQDTK
1060 1070 1080 1090 1100
KSQDLVMKLS NSMNEVVQIT SVLTMIKPFK IFEHELNKPI MSLTGGLSST
1110 1120 1130 1140 1150
TRNDVMWPKS GQGLRESSVM KTLLDERRTS GTQPTTAPVA AEEPRLENVA
1160 1170
LENFVSIGWK LLDDSELGWY
Length:1,170
Mass (Da):128,240
Last modified:October 1, 1993 - v1
Checksum:i2063178C29F8128C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69584 Genomic DNA Translation: CAA49301.1
Z28038 Genomic DNA Translation: CAA81873.1
BK006944 Genomic DNA Translation: DAA09118.1
PIRiS30010
RefSeqiNP_012886.3, NM_001179604.3

Genome annotation databases

EnsemblFungiiYKL038W; YKL038W; YKL038W
GeneIDi853828
KEGGisce:YKL038W

Similar proteinsi

Entry informationi

Entry nameiRGT1_YEAST
AccessioniPrimary (citable) accession number: P32862
Secondary accession number(s): D6VXP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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