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P32861

- UGPA1_YEAST

UniProt

P32861 - UGPA1_YEAST

Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

UGP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Plays a central role as a glucosyl donor in cellular metabolic pathways.1 Publication

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi123 – 1231MagnesiumCurated
    Metal bindingi246 – 2461MagnesiumCurated
    Active sitei388 – 3881Curated

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. uridylyltransferase activity Source: SGD
    4. UTP:glucose-1-phosphate uridylyltransferase activity Source: SGD

    GO - Biological processi

    1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
    2. glycogen biosynthetic process Source: SGD
    3. trehalose biosynthetic process Source: SGD
    4. UDP-glucose metabolic process Source: SGD

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17138.
    YEAST:YKL035W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
    Alternative name(s):
    UDP-glucose pyrophosphorylase
    Short name:
    UDPGP
    Short name:
    UGPase
    Gene namesi
    Name:UGP1
    Ordered Locus Names:YKL035W
    ORF Names:YKL248
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL035w.
    SGDiS000001518. UGP1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 499498UTP--glucose-1-phosphate uridylyltransferasePRO_0000185765Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei17 – 171Phosphoserine1 Publication
    Modified residuei19 – 191Phosphothreonine1 Publication
    Modified residuei21 – 211Phosphoserine1 Publication
    Modified residuei79 – 791Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP32861.
    PaxDbiP32861.
    PeptideAtlasiP32861.
    PRIDEiP32861.

    Expressioni

    Gene expression databases

    GenevestigatoriP32861.

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PSK2Q082173EBI-19987,EBI-9839

    Protein-protein interaction databases

    BioGridi34096. 60 interactions.
    DIPiDIP-4534N.
    IntActiP32861. 17 interactions.
    MINTiMINT-488090.
    STRINGi4932.YKL035W.

    Structurei

    Secondary structure

    1
    499
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 308
    Helixi49 – 6719
    Helixi74 – 763
    Turni82 – 843
    Beta strandi85 – 873
    Helixi88 – 925
    Helixi101 – 1033
    Beta strandi104 – 1096
    Helixi115 – 1173
    Beta strandi121 – 1233
    Helixi134 – 14916
    Beta strandi154 – 1585
    Turni161 – 1633
    Helixi164 – 1718
    Helixi172 – 1743
    Beta strandi176 – 1783
    Beta strandi180 – 1845
    Turni194 – 1963
    Beta strandi202 – 2065
    Beta strandi210 – 2123
    Helixi216 – 2183
    Helixi219 – 2268
    Helixi228 – 2347
    Beta strandi239 – 2435
    Beta strandi247 – 2493
    Helixi254 – 2629
    Beta strandi266 – 2738
    Helixi276 – 2783
    Beta strandi279 – 2813
    Beta strandi283 – 2875
    Beta strandi290 – 2945
    Helixi296 – 2983
    Helixi304 – 3074
    Turni310 – 3123
    Beta strandi315 – 32410
    Helixi325 – 3339
    Helixi364 – 3707
    Beta strandi371 – 3733
    Beta strandi375 – 3795
    Helixi381 – 3833
    Helixi390 – 3967
    Beta strandi401 – 4044
    Beta strandi407 – 4104
    Beta strandi420 – 4234
    Helixi425 – 4273
    Helixi430 – 4367
    Beta strandi446 – 45712
    Beta strandi462 – 4709
    Beta strandi477 – 4793
    Beta strandi484 – 49815

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2I5KX-ray3.10A/B12-499[»]
    ProteinModelPortaliP32861.
    SMRiP32861. Positions 20-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32861.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni448 – 49952OligomerizationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the UDPGP type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG4284.
    GeneTreeiENSGT00390000003174.
    HOGENOMiHOG000113618.
    KOiK00963.
    OMAiPPNGKRC.
    OrthoDBiEOG7KDFKX.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PTHR11952:SF1. PTHR11952:SF1. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000806. UDPGP. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32861-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN    50
    ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN 100
    LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD 150
    SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV 200
    PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA 250
    TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP 300
    KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR 350
    DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL 400
    FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT 450
    ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH 499
    Length:499
    Mass (Da):55,988
    Last modified:October 1, 1993 - v1
    Checksum:i9B9F4BED885E004D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69584 Genomic DNA. Translation: CAA49303.1.
    Z28035 Genomic DNA. Translation: CAA81872.1.
    BK006944 Genomic DNA. Translation: DAA09120.1.
    PIRiS30007.
    RefSeqiNP_012889.3. NM_001179601.3.

    Genome annotation databases

    EnsemblFungiiYKL035W; YKL035W; YKL035W.
    GeneIDi853830.
    KEGGisce:YKL035W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69584 Genomic DNA. Translation: CAA49303.1 .
    Z28035 Genomic DNA. Translation: CAA81872.1 .
    BK006944 Genomic DNA. Translation: DAA09120.1 .
    PIRi S30007.
    RefSeqi NP_012889.3. NM_001179601.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2I5K X-ray 3.10 A/B 12-499 [» ]
    ProteinModelPortali P32861.
    SMRi P32861. Positions 20-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34096. 60 interactions.
    DIPi DIP-4534N.
    IntActi P32861. 17 interactions.
    MINTi MINT-488090.
    STRINGi 4932.YKL035W.

    Proteomic databases

    MaxQBi P32861.
    PaxDbi P32861.
    PeptideAtlasi P32861.
    PRIDEi P32861.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL035W ; YKL035W ; YKL035W .
    GeneIDi 853830.
    KEGGi sce:YKL035W.

    Organism-specific databases

    CYGDi YKL035w.
    SGDi S000001518. UGP1.

    Phylogenomic databases

    eggNOGi COG4284.
    GeneTreei ENSGT00390000003174.
    HOGENOMi HOG000113618.
    KOi K00963.
    OMAi PPNGKRC.
    OrthoDBi EOG7KDFKX.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17138.
    YEAST:YKL035W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32861.
    NextBioi 975029.
    PROi P32861.

    Gene expression databases

    Genevestigatori P32861.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view ]
    PANTHERi PTHR11952. PTHR11952. 1 hit.
    PTHR11952:SF1. PTHR11952:SF1. 1 hit.
    Pfami PF01704. UDPGP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000806. UDPGP. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato."
      Purnelle B., Skala J., van Dyck L., Goffeau A.
      Yeast 8:977-986(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
      Daran J.M., Dallies N., Thines-Sempoux D., Paquet V., Francois J.
      Eur. J. Biochem. 233:520-530(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; THR-19 AND SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    11. "Structural basis for subunit assembly in UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
      Roeben A., Plitzko J.M., Korner R., Bottcher U.M., Siegers K., Hayer-Hartl M., Bracher A.
      J. Mol. Biol. 364:551-560(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-499, SUBUNIT, OLIGOMERIZATION REGION, PROBABLE MAGNESIUM-BINDING SITES.

    Entry informationi

    Entry nameiUGPA1_YEAST
    AccessioniPrimary (citable) accession number: P32861
    Secondary accession number(s): D6VXQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 17200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3