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Reviewed, UniProtKB/Swiss-Prot P32861 (UGPA1_YEAST)

Last modified November 24, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UTP--glucose-1-phosphate uridylyltransferase
    EC=2.7.7.9
Alternative name(s):
    UDP-glucose pyrophosphorylase
      Short name=UGPase
      Short name=UDPGP
Gene names
Name: UGP1
Ordered Locus Names: YKL035W
ORF Names: YKL248
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plays a central role as a glucosyl donor in cellular metabolic pathways. Ref.3

Catalytic activity

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the UDPGP type 1 family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-19987,EBI-19987
TEM1P389871EBI-19987,EBI-19113

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499UTP--glucose-1-phosphate uridylyltransferase
PRO_0000185765

Amino acid modifications

Modified residue91Phosphothreonine Ref.7 Ref.8 Ref.9
Modified residue111Phosphoserine Ref.7 Ref.9 Ref.4 Ref.6
Modified residue121Phosphothreonine Ref.7 Ref.9
Modified residue131Phosphotyrosine Ref.9
Modified residue171Phosphoserine Ref.7
Modified residue191Phosphothreonine Ref.7
Modified residue211Phosphoserine Ref.7
Modified residue791Phosphoserine Ref.9
Modified residue1021Phosphoserine Ref.9
Modified residue1161Phosphoserine Ref.9
Modified residue1831Phosphoserine Ref.8
Modified residue3911Phosphoserine Ref.9

Secondary structure

1 ............................................................................................ 499
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P32861-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 9B9F4BED885E004D

FASTA49955,988
        10         20         30         40         50         60 
MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN ELDSFFTLFR 

        70         80         90        100        110        120 
RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN LSKLAVLKLN GGLGTSMGCV 

       130        140        150        160        170        180 
GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD SDVPLLLMNS FNTDKDTEHL IKKYSANRIR 

       190        200        210        220        230        240 
IRSFNQSRFP RVYKDSLLPV PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL 

       250        260        270        280        290        300 
FVSNGDNLGA TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP 

       310        320        330        340        350        360 
KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR DGHEINVLQL 

       370        380        390        400        410        420 
ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL FRLEHGSLKL DPSRFGPNPL 

       430        440        450        460        470        480 
IKLGSHFKKV SGFNARIPHI PKIVELDHLT ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP 

       490 
NGSILENVVV TGNLQILEH

« Hide

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References

« Hide 'large scale' references
[1]"The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato."
Purnelle B., Skala J., van Dyck L., Goffeau A.
Yeast 8:977-986(1992) [PubMed: 1481573] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
Daran J.M., Dallies N., Thines-Sempoux D., Paquet V., Francois J.
Eur. J. Biochem. 233:520-530(1995) [PubMed: 7588797] [Abstract]
Cited for: FUNCTION.
[4]"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae."
Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M.
Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-9; SER-11; THR-12; SER-17; THR-19 AND SER-21, MASS SPECTROMETRY.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-9 AND SER-183, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-9; SER-11; THR-12; TYR-13; SER-79; SER-102; SER-116 AND SER-391, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X69584 Genomic DNA. Translation: CAA49303.1.
Z28035 Genomic DNA. Translation: CAA81872.1.
PIRS30007.
RefSeqNP_012889.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2I5KX-ray3.10A/B12-499[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4534N.
IntActP32861. 14 interactions.
STRINGP32861.

Proteomic databases

PeptideAtlasP32861.
PRIDEP32861.

Genome annotation databases

EnsemblYKL035W; YKL035W; YKL035W; Saccharomyces cerevisiae. [Genome view]
GeneID853830.
KEGGsce:YKL035W.
NMPDRfig|4932.3.peg.3874.

Organism-specific databases

CYGDYKL035w.
SGDS000001518. UGP1.

Phylogenomic databases

HOGENOMP32861.
OMAAEYIMEL
OrthoDBEOG9DJMD8

Enzyme and pathway databases

BRENDA2.7.7.9. 250.

Gene expression databases

ArrayExpressP32861.
GenevestigatorP32861.
GermOnlineYKL035W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002618. UDPGP_trans.
IPR016267. UDPGP_trans_sub.
[Graphical view]
PANTHERPTHR11952. UDPGP_trans. 1 hit.
PfamPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFPIRSF000806. UDPGP. 1 hit.
ProtoNetSearch...

Other Resources

NextBio975029.

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Entry information

Entry nameUGPA1_YEAST
AccessionPrimary (citable) accession number: P32861
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents