SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32861

- UGPA1_YEAST

UniProt

P32861 - UGPA1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
UTP--glucose-1-phosphate uridylyltransferase
Gene
UGP1, YKL035W, YKL248
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a central role as a glucosyl donor in cellular metabolic pathways.1 Publication

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231Magnesium Inferred
Metal bindingi246 – 2461Magnesium Inferred
Active sitei388 – 3881 Inferred

GO - Molecular functioni

  1. UTP:glucose-1-phosphate uridylyltransferase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. uridylyltransferase activity Source: SGD

GO - Biological processi

  1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
  2. UDP-glucose metabolic process Source: SGD
  3. glycogen biosynthetic process Source: SGD
  4. trehalose biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17138.
YEAST:YKL035W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Short name:
UGPase
Gene namesi
Name:UGP1
Ordered Locus Names:YKL035W
ORF Names:YKL248
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL035w.
SGDiS000001518. UGP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 499498UTP--glucose-1-phosphate uridylyltransferase
PRO_0000185765Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei19 – 191Phosphothreonine1 Publication
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei79 – 791Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32861.
PaxDbiP32861.
PeptideAtlasiP32861.
PRIDEiP32861.

Expressioni

Gene expression databases

GenevestigatoriP32861.

Interactioni

Subunit structurei

Homooctamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PSK2Q082173EBI-19987,EBI-9839

Protein-protein interaction databases

BioGridi34096. 60 interactions.
DIPiDIP-4534N.
IntActiP32861. 17 interactions.
MINTiMINT-488090.
STRINGi4932.YKL035W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 308
Helixi49 – 6719
Helixi74 – 763
Turni82 – 843
Beta strandi85 – 873
Helixi88 – 925
Helixi101 – 1033
Beta strandi104 – 1096
Helixi115 – 1173
Beta strandi121 – 1233
Helixi134 – 14916
Beta strandi154 – 1585
Turni161 – 1633
Helixi164 – 1718
Helixi172 – 1743
Beta strandi176 – 1783
Beta strandi180 – 1845
Turni194 – 1963
Beta strandi202 – 2065
Beta strandi210 – 2123
Helixi216 – 2183
Helixi219 – 2268
Helixi228 – 2347
Beta strandi239 – 2435
Beta strandi247 – 2493
Helixi254 – 2629
Beta strandi266 – 2738
Helixi276 – 2783
Beta strandi279 – 2813
Beta strandi283 – 2875
Beta strandi290 – 2945
Helixi296 – 2983
Helixi304 – 3074
Turni310 – 3123
Beta strandi315 – 32410
Helixi325 – 3339
Helixi364 – 3707
Beta strandi371 – 3733
Beta strandi375 – 3795
Helixi381 – 3833
Helixi390 – 3967
Beta strandi401 – 4044
Beta strandi407 – 4104
Beta strandi420 – 4234
Helixi425 – 4273
Helixi430 – 4367
Beta strandi446 – 45712
Beta strandi462 – 4709
Beta strandi477 – 4793
Beta strandi484 – 49815

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I5KX-ray3.10A/B12-499[»]
ProteinModelPortaliP32861.
SMRiP32861. Positions 20-499.

Miscellaneous databases

EvolutionaryTraceiP32861.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni448 – 49952Oligomerization
Add
BLAST

Sequence similaritiesi

Belongs to the UDPGP type 1 family.

Phylogenomic databases

eggNOGiCOG4284.
GeneTreeiENSGT00390000003174.
HOGENOMiHOG000113618.
KOiK00963.
OMAiPPNGKRC.
OrthoDBiEOG7KDFKX.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PTHR11952:SF1. PTHR11952:SF1. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32861-1 [UniParc]FASTAAdd to Basket

« Hide

MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN    50
ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN 100
LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD 150
SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV 200
PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA 250
TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP 300
KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR 350
DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL 400
FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT 450
ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH 499
Length:499
Mass (Da):55,988
Last modified:October 1, 1993 - v1
Checksum:i9B9F4BED885E004D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1.
Z28035 Genomic DNA. Translation: CAA81872.1.
BK006944 Genomic DNA. Translation: DAA09120.1.
PIRiS30007.
RefSeqiNP_012889.3. NM_001179601.3.

Genome annotation databases

EnsemblFungiiYKL035W; YKL035W; YKL035W.
GeneIDi853830.
KEGGisce:YKL035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1 .
Z28035 Genomic DNA. Translation: CAA81872.1 .
BK006944 Genomic DNA. Translation: DAA09120.1 .
PIRi S30007.
RefSeqi NP_012889.3. NM_001179601.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I5K X-ray 3.10 A/B 12-499 [» ]
ProteinModelPortali P32861.
SMRi P32861. Positions 20-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34096. 60 interactions.
DIPi DIP-4534N.
IntActi P32861. 17 interactions.
MINTi MINT-488090.
STRINGi 4932.YKL035W.

Proteomic databases

MaxQBi P32861.
PaxDbi P32861.
PeptideAtlasi P32861.
PRIDEi P32861.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL035W ; YKL035W ; YKL035W .
GeneIDi 853830.
KEGGi sce:YKL035W.

Organism-specific databases

CYGDi YKL035w.
SGDi S000001518. UGP1.

Phylogenomic databases

eggNOGi COG4284.
GeneTreei ENSGT00390000003174.
HOGENOMi HOG000113618.
KOi K00963.
OMAi PPNGKRC.
OrthoDBi EOG7KDFKX.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17138.
YEAST:YKL035W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32861.
NextBioi 975029.
PROi P32861.

Gene expression databases

Genevestigatori P32861.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view ]
PANTHERi PTHR11952. PTHR11952. 1 hit.
PTHR11952:SF1. PTHR11952:SF1. 1 hit.
Pfami PF01704. UDPGP. 1 hit.
[Graphical view ]
PIRSFi PIRSF000806. UDPGP. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato."
    Purnelle B., Skala J., van Dyck L., Goffeau A.
    Yeast 8:977-986(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
    Daran J.M., Dallies N., Thines-Sempoux D., Paquet V., Francois J.
    Eur. J. Biochem. 233:520-530(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; THR-19 AND SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "Structural basis for subunit assembly in UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
    Roeben A., Plitzko J.M., Korner R., Bottcher U.M., Siegers K., Hayer-Hartl M., Bracher A.
    J. Mol. Biol. 364:551-560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-499, SUBUNIT, OLIGOMERIZATION REGION, PROBABLE MAGNESIUM-BINDING SITES.

Entry informationi

Entry nameiUGPA1_YEAST
AccessioniPrimary (citable) accession number: P32861
Secondary accession number(s): D6VXQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi