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P32861

- UGPA1_YEAST

UniProt

P32861 - UGPA1_YEAST

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Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

UGP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a central role as a glucosyl donor in cellular metabolic pathways.1 Publication

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231MagnesiumCurated
Metal bindingi246 – 2461MagnesiumCurated
Active sitei388 – 3881Curated

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. uridylyltransferase activity Source: SGD
  3. UTP:glucose-1-phosphate uridylyltransferase activity Source: SGD

GO - Biological processi

  1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
  2. glycogen biosynthetic process Source: SGD
  3. trehalose biosynthetic process Source: SGD
  4. UDP-glucose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17138.
YEAST:YKL035W-MONOMER.
ReactomeiREACT_191292. Glycogen synthesis.
REACT_241495. Formation of the active cofactor, UDP-glucuronate.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Short name:
UGPase
Gene namesi
Name:UGP1
Ordered Locus Names:YKL035W
ORF Names:YKL248
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL035w.
SGDiS000001518. UGP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 499498UTP--glucose-1-phosphate uridylyltransferasePRO_0000185765Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei19 – 191Phosphothreonine1 Publication
Modified residuei21 – 211Phosphoserine1 Publication
Modified residuei79 – 791Phosphoserine1 Publication
Modified residuei369 – 3691Omega-N-methylarginine1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP32861.
PaxDbiP32861.
PeptideAtlasiP32861.
PRIDEiP32861.

Expressioni

Gene expression databases

GenevestigatoriP32861.

Interactioni

Subunit structurei

Homooctamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PSK2Q082173EBI-19987,EBI-9839

Protein-protein interaction databases

BioGridi34096. 61 interactions.
DIPiDIP-4534N.
IntActiP32861. 17 interactions.
MINTiMINT-488090.
STRINGi4932.YKL035W.

Structurei

Secondary structure

1
499
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 308Combined sources
Helixi49 – 6719Combined sources
Helixi74 – 763Combined sources
Turni82 – 843Combined sources
Beta strandi85 – 873Combined sources
Helixi88 – 925Combined sources
Helixi101 – 1033Combined sources
Beta strandi104 – 1096Combined sources
Helixi115 – 1173Combined sources
Beta strandi121 – 1233Combined sources
Helixi134 – 14916Combined sources
Beta strandi154 – 1585Combined sources
Turni161 – 1633Combined sources
Helixi164 – 1718Combined sources
Helixi172 – 1743Combined sources
Beta strandi176 – 1783Combined sources
Beta strandi180 – 1845Combined sources
Turni194 – 1963Combined sources
Beta strandi202 – 2065Combined sources
Beta strandi210 – 2123Combined sources
Helixi216 – 2183Combined sources
Helixi219 – 2268Combined sources
Helixi228 – 2347Combined sources
Beta strandi239 – 2435Combined sources
Beta strandi247 – 2493Combined sources
Helixi254 – 2629Combined sources
Beta strandi266 – 2738Combined sources
Helixi276 – 2783Combined sources
Beta strandi279 – 2813Combined sources
Beta strandi283 – 2875Combined sources
Beta strandi290 – 2945Combined sources
Helixi296 – 2983Combined sources
Helixi304 – 3074Combined sources
Turni310 – 3123Combined sources
Beta strandi315 – 32410Combined sources
Helixi325 – 3339Combined sources
Helixi364 – 3707Combined sources
Beta strandi371 – 3733Combined sources
Beta strandi375 – 3795Combined sources
Helixi381 – 3833Combined sources
Helixi390 – 3967Combined sources
Beta strandi401 – 4044Combined sources
Beta strandi407 – 4104Combined sources
Beta strandi420 – 4234Combined sources
Helixi425 – 4273Combined sources
Helixi430 – 4367Combined sources
Beta strandi446 – 45712Combined sources
Beta strandi462 – 4709Combined sources
Beta strandi477 – 4793Combined sources
Beta strandi484 – 49815Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I5KX-ray3.10A/B12-499[»]
ProteinModelPortaliP32861.
SMRiP32861. Positions 20-499.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32861.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni448 – 49952OligomerizationAdd
BLAST

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiCOG4284.
GeneTreeiENSGT00390000003174.
HOGENOMiHOG000113618.
InParanoidiP32861.
KOiK00963.
OMAiPPNGKRC.
OrthoDBiEOG7KDFKX.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PTHR11952:SF1. PTHR11952:SF1. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32861-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN
60 70 80 90 100
ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN
110 120 130 140 150
LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD
160 170 180 190 200
SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV
210 220 230 240 250
PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA
260 270 280 290 300
TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP
310 320 330 340 350
KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR
360 370 380 390 400
DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL
410 420 430 440 450
FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT
460 470 480 490
ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH
Length:499
Mass (Da):55,988
Last modified:October 1, 1993 - v1
Checksum:i9B9F4BED885E004D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1.
Z28035 Genomic DNA. Translation: CAA81872.1.
BK006944 Genomic DNA. Translation: DAA09120.1.
PIRiS30007.
RefSeqiNP_012889.3. NM_001179601.3.

Genome annotation databases

EnsemblFungiiYKL035W; YKL035W; YKL035W.
GeneIDi853830.
KEGGisce:YKL035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1 .
Z28035 Genomic DNA. Translation: CAA81872.1 .
BK006944 Genomic DNA. Translation: DAA09120.1 .
PIRi S30007.
RefSeqi NP_012889.3. NM_001179601.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I5K X-ray 3.10 A/B 12-499 [» ]
ProteinModelPortali P32861.
SMRi P32861. Positions 20-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34096. 61 interactions.
DIPi DIP-4534N.
IntActi P32861. 17 interactions.
MINTi MINT-488090.
STRINGi 4932.YKL035W.

Proteomic databases

MaxQBi P32861.
PaxDbi P32861.
PeptideAtlasi P32861.
PRIDEi P32861.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL035W ; YKL035W ; YKL035W .
GeneIDi 853830.
KEGGi sce:YKL035W.

Organism-specific databases

CYGDi YKL035w.
SGDi S000001518. UGP1.

Phylogenomic databases

eggNOGi COG4284.
GeneTreei ENSGT00390000003174.
HOGENOMi HOG000113618.
InParanoidi P32861.
KOi K00963.
OMAi PPNGKRC.
OrthoDBi EOG7KDFKX.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17138.
YEAST:YKL035W-MONOMER.
Reactomei REACT_191292. Glycogen synthesis.
REACT_241495. Formation of the active cofactor, UDP-glucuronate.

Miscellaneous databases

EvolutionaryTracei P32861.
NextBioi 975029.
PROi P32861.

Gene expression databases

Genevestigatori P32861.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view ]
PANTHERi PTHR11952. PTHR11952. 1 hit.
PTHR11952:SF1. PTHR11952:SF1. 1 hit.
Pfami PF01704. UDPGP. 1 hit.
[Graphical view ]
PIRSFi PIRSF000806. UDPGP. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato."
    Purnelle B., Skala J., van Dyck L., Goffeau A.
    Yeast 8:977-986(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
    Daran J.M., Dallies N., Thines-Sempoux D., Paquet V., Francois J.
    Eur. J. Biochem. 233:520-530(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; THR-19 AND SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "Analysis of the proteome of Saccharomyces cerevisiae for methylarginine."
    Low J.K., Hart-Smith G., Erce M.A., Wilkins M.R.
    J. Proteome Res. 12:3884-3899(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ARG-369.
  12. "Structural basis for subunit assembly in UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae."
    Roeben A., Plitzko J.M., Korner R., Bottcher U.M., Siegers K., Hayer-Hartl M., Bracher A.
    J. Mol. Biol. 364:551-560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-499, SUBUNIT, OLIGOMERIZATION REGION, PROBABLE MAGNESIUM-BINDING SITES.

Entry informationi

Entry nameiUGPA1_YEAST
AccessioniPrimary (citable) accession number: P32861
Secondary accession number(s): D6VXQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3