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Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

UGP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role as a glucosyl donor in cellular metabolic pathways.1 Publication

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi123MagnesiumCurated1
Binding sitei123UTPBy similarity1
Binding sitei186UTPBy similarity1
Binding sitei215UTP; via amide nitrogenBy similarity1
Binding sitei216SubstrateBy similarity1
Metal bindingi246MagnesiumCurated1
Binding sitei246UTPBy similarity1
Active sitei388Curated1
Binding sitei388UTPBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • uridylyltransferase activity Source: SGD
  • UTP:glucose-1-phosphate uridylyltransferase activity Source: SGD

GO - Biological processi

  • (1->6)-beta-D-glucan biosynthetic process Source: SGD
  • glycogen biosynthetic process Source: SGD
  • trehalose biosynthetic process Source: SGD
  • UDP-glucose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YKL035W-MONOMER.
YEAST:YKL035W-MONOMER.
ReactomeiR-SCE-173599. Formation of the active cofactor, UDP-glucuronate.
R-SCE-3322077. Glycogen synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Short name:
UGPase
Gene namesi
Name:UGP1
Ordered Locus Names:YKL035W
ORF Names:YKL248
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL035W.
SGDiS000001518. UGP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001857652 – 499UTP--glucose-1-phosphate uridylyltransferaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei17PhosphoserineCombined sources1
Modified residuei19PhosphothreonineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei369Omega-N-methylarginine1 Publication1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP32861.
PRIDEiP32861.

PTM databases

iPTMnetiP32861.

Interactioni

Subunit structurei

Homooctamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PSK2Q082173EBI-19987,EBI-9839

Protein-protein interaction databases

BioGridi34096. 69 interactors.
DIPiDIP-4534N.
IntActiP32861. 17 interactors.
MINTiMINT-488090.

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 30Combined sources8
Helixi49 – 67Combined sources19
Helixi74 – 76Combined sources3
Turni82 – 84Combined sources3
Beta strandi85 – 87Combined sources3
Helixi88 – 92Combined sources5
Helixi101 – 103Combined sources3
Beta strandi104 – 109Combined sources6
Helixi115 – 117Combined sources3
Beta strandi121 – 123Combined sources3
Helixi134 – 149Combined sources16
Beta strandi154 – 158Combined sources5
Turni161 – 163Combined sources3
Helixi164 – 171Combined sources8
Helixi172 – 174Combined sources3
Beta strandi176 – 178Combined sources3
Beta strandi180 – 184Combined sources5
Turni194 – 196Combined sources3
Beta strandi202 – 206Combined sources5
Beta strandi210 – 212Combined sources3
Helixi216 – 218Combined sources3
Helixi219 – 226Combined sources8
Helixi228 – 234Combined sources7
Beta strandi239 – 243Combined sources5
Beta strandi247 – 249Combined sources3
Helixi254 – 262Combined sources9
Beta strandi266 – 273Combined sources8
Helixi276 – 278Combined sources3
Beta strandi279 – 281Combined sources3
Beta strandi283 – 287Combined sources5
Beta strandi290 – 294Combined sources5
Helixi296 – 298Combined sources3
Helixi304 – 307Combined sources4
Turni310 – 312Combined sources3
Beta strandi315 – 324Combined sources10
Helixi325 – 333Combined sources9
Helixi364 – 370Combined sources7
Beta strandi371 – 373Combined sources3
Beta strandi375 – 379Combined sources5
Helixi381 – 383Combined sources3
Helixi390 – 396Combined sources7
Beta strandi401 – 404Combined sources4
Beta strandi407 – 410Combined sources4
Beta strandi420 – 423Combined sources4
Helixi425 – 427Combined sources3
Helixi430 – 436Combined sources7
Beta strandi446 – 457Combined sources12
Beta strandi462 – 470Combined sources9
Beta strandi477 – 479Combined sources3
Beta strandi484 – 498Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I5KX-ray3.10A/B12-499[»]
ProteinModelPortaliP32861.
SMRiP32861.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32861.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 112UTP bindingBy similarity4
Regioni111 – 112Substrate bindingBy similarity2
Regioni244 – 246Substrate bindingBy similarity3
Regioni448 – 499OligomerizationAdd BLAST52

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003174.
HOGENOMiHOG000113618.
InParanoidiP32861.
KOiK00963.
OMAiAVDWDRI.
OrthoDBiEOG092C1XSD.

Family and domain databases

CDDicd00897. UGPase_euk. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32861-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN
60 70 80 90 100
ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN
110 120 130 140 150
LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD
160 170 180 190 200
SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV
210 220 230 240 250
PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA
260 270 280 290 300
TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP
310 320 330 340 350
KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR
360 370 380 390 400
DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL
410 420 430 440 450
FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT
460 470 480 490
ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH
Length:499
Mass (Da):55,988
Last modified:October 1, 1993 - v1
Checksum:i9B9F4BED885E004D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1.
Z28035 Genomic DNA. Translation: CAA81872.1.
BK006944 Genomic DNA. Translation: DAA09120.1.
PIRiS30007.
RefSeqiNP_012889.3. NM_001179601.3.

Genome annotation databases

EnsemblFungiiYKL035W; YKL035W; YKL035W.
GeneIDi853830.
KEGGisce:YKL035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69584 Genomic DNA. Translation: CAA49303.1.
Z28035 Genomic DNA. Translation: CAA81872.1.
BK006944 Genomic DNA. Translation: DAA09120.1.
PIRiS30007.
RefSeqiNP_012889.3. NM_001179601.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I5KX-ray3.10A/B12-499[»]
ProteinModelPortaliP32861.
SMRiP32861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34096. 69 interactors.
DIPiDIP-4534N.
IntActiP32861. 17 interactors.
MINTiMINT-488090.

PTM databases

iPTMnetiP32861.

Proteomic databases

MaxQBiP32861.
PRIDEiP32861.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL035W; YKL035W; YKL035W.
GeneIDi853830.
KEGGisce:YKL035W.

Organism-specific databases

EuPathDBiFungiDB:YKL035W.
SGDiS000001518. UGP1.

Phylogenomic databases

GeneTreeiENSGT00390000003174.
HOGENOMiHOG000113618.
InParanoidiP32861.
KOiK00963.
OMAiAVDWDRI.
OrthoDBiEOG092C1XSD.

Enzyme and pathway databases

BioCyciMetaCyc:YKL035W-MONOMER.
YEAST:YKL035W-MONOMER.
ReactomeiR-SCE-173599. Formation of the active cofactor, UDP-glucuronate.
R-SCE-3322077. Glycogen synthesis.

Miscellaneous databases

EvolutionaryTraceiP32861.
PROiP32861.

Family and domain databases

CDDicd00897. UGPase_euk. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUGPA1_YEAST
AccessioniPrimary (citable) accession number: P32861
Secondary accession number(s): D6VXQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.