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Protein

NifU-like protein, mitochondrial

Gene

NFU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in iron homeostasis within the mitochondrion where it is involved in the assembly of iron-sulfur proteins (PubMed:10468587, PubMed:27532773). Together with BOL3, required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein (PubMed:27532773). Required for protecting iron sulfur clusters from oxidative damage (PubMed:27532773).2 Publications

Miscellaneous

Present with 11300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • intracellular sequestering of iron ion Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • protein maturation by iron-sulfur cluster transfer Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-31841-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NifU-like protein, mitochondrial
Gene namesi
Name:NFU1
Synonyms:NUB1
Ordered Locus Names:YKL040C
ORF Names:YKL253
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL040C.
SGDiS000001523. NFU1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Cells are impaired in growth on synthetic complete medium with acetate as a carbon source due to defects in mitochondrial [4Fe-4S] cluster formation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194G → C: Dominant negative mutant; cells show a severe synthetic sick phenotype on glycerol/lactate medium. 1 Publication1
Mutagenesisi196 – 199CTSC → ATSA: Loss of function. Abolished homodimerization. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000019702? – 256NifU-like protein, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP32860.
PRIDEiP32860.

PTM databases

iPTMnetiP32860.

Interactioni

Subunit structurei

Homodimer; in absence of BOL3, probably bridged by an iron-sulfure cluster (PubMed:27532773). Interacts with BOL3 (PubMed:27532773). Interacts with apo-target proteins, such as ACO1, LYS4, ACO2 and SDH2 (PubMed:27532773).1 Publication

Protein-protein interaction databases

BioGridi34092. 203 interactors.
IntActiP32860. 2 interactors.
MINTiMINT-2787395.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 27Combined sources4
Beta strandi32 – 39Combined sources8
Beta strandi53 – 58Combined sources6
Helixi60 – 65Combined sources6
Helixi67 – 75Combined sources9
Beta strandi79 – 84Combined sources6
Beta strandi86 – 93Combined sources8
Helixi99 – 116Combined sources18
Beta strandi118 – 122Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LTLNMR-A17-124[»]
ProteinModelPortaliP32860.
SMRiP32860.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi196 – 199CxxC motif1 Publication4

Domaini

The CxxC motif may bind a [4Fe-4S] cluster.1 Publication

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000011296.
HOGENOMiHOG000023557.
InParanoidiP32860.
OMAiQCPGVES.
OrthoDBiEOG092C4DND.

Family and domain databases

Gene3Di3.30.1370.70. 1 hit.
InterProiView protein in InterPro
IPR014824. Nfu/NifU_N.
IPR001075. NIF_FeS_clus_asmbl_NifU_C.
PfamiView protein in Pfam
PF08712. Nfu_N. 1 hit.
PF01106. NifU. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit.
SMARTiView protein in SMART
SM00932. Nfu_N. 1 hit.
SUPFAMiSSF110836. SSF110836. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKSVAKLGK SPIFYLNSQR LIHIKTLTTP NENALKFLST DGEMLQTRGS
60 70 80 90 100
KSIVIKNTDE NLINHSKLAQ QIFLQCPGVE SLMIGDDFLT INKDRMVHWN
110 120 130 140 150
SIKPEIIDLL TKQLAYGEDV ISKEFHAVQE EEGEGGYKIN MPKFELTEED
160 170 180 190 200
EEVSELIEEL IDTRIRPAIL EDGGDIDYRG WDPKTGTVYL RLQGACTSCS
210 220 230 240 250
SSEVTLKYGI ESMLKHYVDE VKEVIQIMDP EQEIALKEFD KLEKKLESSK

NTSHEK
Length:256
Mass (Da):29,174
Last modified:June 1, 1994 - v2
Checksum:i4EF11EFD9C438BB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28040 Genomic DNA. Translation: CAA81875.1.
AY557904 Genomic DNA. Translation: AAS56230.1.
X69584 Genomic DNA. Translation: CAA49299.1.
BK006944 Genomic DNA. Translation: DAA09116.1.
PIRiS37861.
RefSeqiNP_012884.3. NM_001179606.3.

Genome annotation databases

EnsemblFungiiYKL040C; YKL040C; YKL040C.
GeneIDi853826.
KEGGisce:YKL040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28040 Genomic DNA. Translation: CAA81875.1.
AY557904 Genomic DNA. Translation: AAS56230.1.
X69584 Genomic DNA. Translation: CAA49299.1.
BK006944 Genomic DNA. Translation: DAA09116.1.
PIRiS37861.
RefSeqiNP_012884.3. NM_001179606.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LTLNMR-A17-124[»]
ProteinModelPortaliP32860.
SMRiP32860.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34092. 203 interactors.
IntActiP32860. 2 interactors.
MINTiMINT-2787395.

PTM databases

iPTMnetiP32860.

Proteomic databases

MaxQBiP32860.
PRIDEiP32860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL040C; YKL040C; YKL040C.
GeneIDi853826.
KEGGisce:YKL040C.

Organism-specific databases

EuPathDBiFungiDB:YKL040C.
SGDiS000001523. NFU1.

Phylogenomic databases

GeneTreeiENSGT00390000011296.
HOGENOMiHOG000023557.
InParanoidiP32860.
OMAiQCPGVES.
OrthoDBiEOG092C4DND.

Enzyme and pathway databases

BioCyciYEAST:G3O-31841-MONOMER.

Miscellaneous databases

PROiPR:P32860.

Family and domain databases

Gene3Di3.30.1370.70. 1 hit.
InterProiView protein in InterPro
IPR014824. Nfu/NifU_N.
IPR001075. NIF_FeS_clus_asmbl_NifU_C.
PfamiView protein in Pfam
PF08712. Nfu_N. 1 hit.
PF01106. NifU. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002830. NIF_FeS_clus_asmbl_NifU_C. 1 hit.
SMARTiView protein in SMART
SM00932. Nfu_N. 1 hit.
SUPFAMiSSF110836. SSF110836. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNFU1_YEAST
AccessioniPrimary (citable) accession number: P32860
Secondary accession number(s): D6VXP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: March 15, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.