P32857 (PTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane protein PTM1

Gene names

Name:	PTM1
Ordered Locus Names:	YKL039W
ORF Names:	YKL252

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Note: Copurifies with the late Golgi SNARE TLG2. Ref.6
Sequence similarities	Belongs to the LU7TM family.
Sequence caution	The sequence CAA49300.1 differs from that shown. Reason: Frameshift at position 513. The sequence CAA81874.1 differs from that shown. Reason: Frameshift at position 513.

Ontologies

Keywords
Cellular component	Endosome Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 523	497	Membrane protein PTM1		PRO_0000022180
Regions
	Topological domain	27 – 197	171	Lumenal Potential
	Transmembrane	198 – 218	21	Helical; Name=1; Potential
	Topological domain	219 – 230	12	Cytoplasmic Potential
	Transmembrane	231 – 251	21	Helical; Name=2; Potential
	Topological domain	252 – 265	14	Lumenal Potential
	Transmembrane	266 – 286	21	Helical; Name=3; Potential
	Topological domain	287 – 304	18	Cytoplasmic Potential
	Transmembrane	305 – 325	21	Helical; Name=4; Potential
	Topological domain	326 – 333	8	Lumenal Potential
	Transmembrane	334 – 354	21	Helical; Name=5; Potential
	Topological domain	355 – 381	27	Cytoplasmic Potential
	Transmembrane	382 – 402	21	Helical; Name=6; Potential
	Topological domain	403 – 417	15	Lumenal Potential
	Transmembrane	418 – 438	21	Helical; Name=7; Potential
	Topological domain	439 – 523	85	Cytoplasmic Potential
Amino acid modifications
	Modified residue	480	1	Phosphoserine Ref.7
	Modified residue	483	1	Phosphothreonine Ref.7
	Modified residue	498	1	Phosphothreonine Ref.7
	Glycosylation	132	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P32857 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: 86E7A339FE192DEC	FASTA	523	60,018
	10 20 30 40 50 60 MRVYQFCRPF QLFTCFLCYL LVFVKANKEK ISQKNYQVCA GMYSKEDWKG KIDPFISFNL 70 80 90 100 110 120 KKISGLSDES DPGLVVAIYD FQDFEHLGVQ LPDEEMYYIC DDYAIDIGIC EEENRDEFIV 130 140 150 160 170 180 QDVVYDPYTS TNRSLANPIM TFSQNEVGLH DTRYPIKETG FYCVTAFRSS TSTKFNAVVN 190 200 210 220 230 240 FRNAYGQLAG TEINKLPLYG LLAVAYVVAM ALYSFAFWKH KHELLPLQKY LLAFFVFLTA 250 260 270 280 290 300 ETIFVWAYYD LKNEKGDTAG IKVYMVFLSI LTAGKVTFSF FLLLIIALGY GIVYPKLNKT 310 320 330 340 350 360 LMRRCQMYGA LTYAICIGFL IQSYLTDMEA PSPLILITLI PMALALIIFY YMIIRSMTKT 370 380 390 400 410 420 VIYLKEQRQI VKLNMYKKLL YIIYASFLSV LAGSIVSSFI YVGMNTIDMI EKNWRSRFFV 430 440 450 460 470 480 TDFWPTLVYF IVFVTIAFLW RPTDTSYMLA ASQQLPTDPE NVADFDLGDL QSFDDQDDAS 490 500 510 520 IITGERGIDE DDLNLNFTDD EEGHDNVNNH SQGHGPVSPS PTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PTM1, a putative transmembrane protein, enhances recovery from transformation of a profilin deletion strain." Haarer B.K., Petzold A.S., Brown S.S. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato." Purnelle B., Skala J., van Dyck L., Goffeau A. Yeast 8:977-986(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480; THR-483 AND THR-498, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11895 Genomic DNA. Translation: AAA34921.1. X69584 Genomic DNA. Translation: CAA49300.1. Frameshift. Z28039 Genomic DNA. Translation: CAA81874.1. Frameshift. BK006944 Genomic DNA. Translation: DAA09117.1.
PIR	S30011.
RefSeq	NP_012885.2. NM_001179605.1.
3D structure databases
ProteinModelPortal	P32857.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1244N.
IntAct	P32857. 14 interactions.
MINT	MINT-404939.
STRING	4932.YKL039W.
Proteomic databases
PaxDb	P32857.
PeptideAtlas	P32857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL039W; YKL039W; YKL039W.
GeneID	853827.
KEGG	sce:YKL039W.
Organism-specific databases
SGD	S000001522. PTM1.
Phylogenomic databases
eggNOG	NOG302490.
GeneTree	ENSGT00390000015417.
HOGENOM	HOG000176489.
OMA	PLQKYLL.
OrthoDB	EOG43XZCR.
Gene expression databases
Genevestigator	P32857.
GermOnline	YKL039W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009637. TM_rcpt_euk. [Graphical view]
PANTHER	PTHR21229. PTHR21229. 1 hit.
Pfam	PF06814. Lung_7-TM_R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975020.

Entry information

Entry name

PTM1_YEAST

Accession

Primary (citable) accession number: P32857
Secondary accession number(s): D6VXP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 1, 2008
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program