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P32857 (PTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Membrane protein PTM1
Gene names
Name:PTM1
Ordered Locus Names:YKL039W
ORF Names:YKL252
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Note: Copurifies with the late Golgi SNARE TLG2. Ref.6

Sequence similarities

Belongs to the LU7TM family.

Sequence caution

The sequence CAA49300.1 differs from that shown. Reason: Frameshift at position 513.

The sequence CAA81874.1 differs from that shown. Reason: Frameshift at position 513.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 523497Membrane protein PTM1
PRO_0000022180

Regions

Topological domain27 – 197171Lumenal Potential
Transmembrane198 – 21821Helical; Name=1; Potential
Topological domain219 – 23012Cytoplasmic Potential
Transmembrane231 – 25121Helical; Name=2; Potential
Topological domain252 – 26514Lumenal Potential
Transmembrane266 – 28621Helical; Name=3; Potential
Topological domain287 – 30418Cytoplasmic Potential
Transmembrane305 – 32521Helical; Name=4; Potential
Topological domain326 – 3338Lumenal Potential
Transmembrane334 – 35421Helical; Name=5; Potential
Topological domain355 – 38127Cytoplasmic Potential
Transmembrane382 – 40221Helical; Name=6; Potential
Topological domain403 – 41715Lumenal Potential
Transmembrane418 – 43821Helical; Name=7; Potential
Topological domain439 – 52385Cytoplasmic Potential

Amino acid modifications

Modified residue4801Phosphoserine Ref.7
Modified residue4831Phosphothreonine Ref.7
Modified residue4981Phosphothreonine Ref.7
Glycosylation1321N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P32857 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: 86E7A339FE192DEC

FASTA52360,018
        10         20         30         40         50         60 
MRVYQFCRPF QLFTCFLCYL LVFVKANKEK ISQKNYQVCA GMYSKEDWKG KIDPFISFNL 

        70         80         90        100        110        120 
KKISGLSDES DPGLVVAIYD FQDFEHLGVQ LPDEEMYYIC DDYAIDIGIC EEENRDEFIV 

       130        140        150        160        170        180 
QDVVYDPYTS TNRSLANPIM TFSQNEVGLH DTRYPIKETG FYCVTAFRSS TSTKFNAVVN 

       190        200        210        220        230        240 
FRNAYGQLAG TEINKLPLYG LLAVAYVVAM ALYSFAFWKH KHELLPLQKY LLAFFVFLTA 

       250        260        270        280        290        300 
ETIFVWAYYD LKNEKGDTAG IKVYMVFLSI LTAGKVTFSF FLLLIIALGY GIVYPKLNKT 

       310        320        330        340        350        360 
LMRRCQMYGA LTYAICIGFL IQSYLTDMEA PSPLILITLI PMALALIIFY YMIIRSMTKT 

       370        380        390        400        410        420 
VIYLKEQRQI VKLNMYKKLL YIIYASFLSV LAGSIVSSFI YVGMNTIDMI EKNWRSRFFV 

       430        440        450        460        470        480 
TDFWPTLVYF IVFVTIAFLW RPTDTSYMLA ASQQLPTDPE NVADFDLGDL QSFDDQDDAS 

       490        500        510        520 
IITGERGIDE DDLNLNFTDD EEGHDNVNNH SQGHGPVSPS PTK 

« Hide

References

« Hide 'large scale' references
[1]"PTM1, a putative transmembrane protein, enhances recovery from transformation of a profilin deletion strain."
Haarer B.K., Petzold A.S., Brown S.S.
Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato."
Purnelle B., Skala J., van Dyck L., Goffeau A.
Yeast 8:977-986(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
Inadome H., Noda Y., Adachi H., Yoda K.
Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480; THR-483 AND THR-498, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11895 Genomic DNA. Translation: AAA34921.1.
X69584 Genomic DNA. Translation: CAA49300.1. Frameshift.
Z28039 Genomic DNA. Translation: CAA81874.1. Frameshift.
BK006944 Genomic DNA. Translation: DAA09117.1.
PIRS30011.
RefSeqNP_012885.2. NM_001179605.1.

3D structure databases

ProteinModelPortalP32857.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34093. 35 interactions.
DIPDIP-1244N.
IntActP32857. 14 interactions.
MINTMINT-404939.
STRING4932.YKL039W.

Proteomic databases

MaxQBP32857.
PaxDbP32857.
PeptideAtlasP32857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL039W; YKL039W; YKL039W.
GeneID853827.
KEGGsce:YKL039W.

Organism-specific databases

SGDS000001522. PTM1.

Phylogenomic databases

eggNOGNOG302490.
GeneTreeENSGT00390000015417.
HOGENOMHOG000176489.
OMAFAMSDEI.
OrthoDBEOG70ZZWZ.

Enzyme and pathway databases

BioCycYEAST:G3O-31840-MONOMER.

Gene expression databases

GenevestigatorP32857.

Family and domain databases

InterProIPR009637. TM_rcpt_euk.
[Graphical view]
PANTHERPTHR21229. PTHR21229. 1 hit.
PfamPF06814. Lung_7-TM_R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975020.

Entry information

Entry namePTM1_YEAST
AccessionPrimary (citable) accession number: P32857
Secondary accession number(s): D6VXP7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families